{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T06:53:52Z","timestamp":1771916032241,"version":"3.50.1"},"reference-count":50,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T00:00:00Z","timestamp":1673827200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Funds Regional Development Fund (ERDF)","award":["1420-26"],"award-info":[{"award-number":["1420-26"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Artificial insemination (AI) with frozen semen in goats still presents serious difficulties, especially in certain goat breeds, in spite of technological progress. The aim of this work is the in vitro study of seminal extenders adapted from those used on other species to evaluate the response of goat sperm to several homeostatic conditions in order to achieve optimal post-thaw semen quality. Three different extenders based on different activity principles were used: (1) extender according to the methodology proposed for pigs, (2) skimmed-milk-based extender according to the methodology proposed for goats in France, and (3) a new egg-yolk-based extender replacing membrane-protective surfactants with sodium dodecyl sulfate (SDS) and named by our team as extender \u201cIMIDA\u201d. The freezing guidelines were those proposed for the freezing of porcine semen. The results obtained show that the egg-yolk-based extenders have good parameters of sperm motility at thawing, studied objectively using the computer-assisted sperm analysis (CASA) system and also subjectively. In particular, in the sperm resistance test after five hours, the thawed sperm containing SDS in their composition showed an optimal average on every evaluated parameter. The new IMIDA extender provided the highest sperm quality averages, so it could be a good extender to use in cryopreservation of semen in the caprine species.<\/jats:p>","DOI":"10.3390\/ani13020309","type":"journal-article","created":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T03:40:34Z","timestamp":1673840434000},"page":"309","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Post-Thaw Quality of Spermatozoa Frozen with Three Different Extenders in the Murciano Granadina Goat Breed"],"prefix":"10.3390","volume":"13","author":[{"given":"Sonia","family":"Gali\u00e1n","sequence":"first","affiliation":[{"name":"Murcian Institute of Agricultural and Environmental Research and Development, Calle Mayor s\/n, La Alberca, 30 150 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bego\u00f1a","family":"Peinado","sequence":"additional","affiliation":[{"name":"Murcian Institute of Agricultural and Environmental Research and Development, Calle Mayor s\/n, La Alberca, 30 150 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Almela","sequence":"additional","affiliation":[{"name":"Murcian Institute of Agricultural and Environmental Research and Development, Calle Mayor s\/n, La Alberca, 30 150 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1ngel","family":"Poto","sequence":"additional","affiliation":[{"name":"Murcian Institute of Agricultural and Environmental Research and Development, Calle Mayor s\/n, La Alberca, 30 150 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5713-485X","authenticated-orcid":false,"given":"Salvador","family":"Ruiz","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Veterinary Medicine, University of Murcia, 30 071 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,16]]},"reference":[{"key":"ref_1","unstructured":"V\u00e1zquez, I., Tapia, A., Herrera, J., Serrano, M.A., S\u00e1nchez, B., and Fischer, P. (2001). Effect of a frozen method on the viability results of goat spermatozoa (Murciano-Granadina). Congresos y Jornadas, JA, CAP."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0921-4488(99)00165-0","article-title":"Synchronization of oestrus in goats: Dose effect of progestagen","volume":"36","author":"Greyling","year":"2000","journal-title":"Small Rumin. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.smallrumres.2003.10.015","article-title":"Syncronization of estrus using CIDR, FGA or MAP intravaginal pessaries during the season breeding in Nubian goats","volume":"55","author":"Romano","year":"2004","journal-title":"Small Rumin. Res."},{"key":"ref_4","unstructured":"Lorenzo, M., Fresno, M., Darmain, N., Molina, A., and Ramos, R. (1997). Estudio del comportamiento reproductivo y calidad seminal de los machos y de la fertilidad mediante inseminaci\u00f3n artificial en la Agrupaci\u00f3n caprina Canaria. XXII Jornadas Cient\u00edficas SEOC. Avances en Alimentaci\u00f3n y Mejora Animal, Agricultura Ganader\u00eda y Pesca Gobierno de Canarias."},{"key":"ref_5","first-page":"4","article-title":"Inseminaci\u00f3n artificial con semen congelado en cabras de raza Angora","volume":"162","author":"Gibbons","year":"2002","journal-title":"Taurus"},{"key":"ref_6","first-page":"273","article-title":"Incidencia del tipo de servicio sobre par\u00e1metros reproductivos de cabras criollas en Los Llanos de La Rioja","volume":"24","author":"Vera","year":"2004","journal-title":"Rev. Argent. Prod. Anim."},{"key":"ref_7","unstructured":"Ni\u00f1o-Gonz\u00e1lez, T. (2021, March 03). Congelaci\u00f3n y Conservaci\u00f3n del Semen en la Especie Caprina Mediante la Utilizaci\u00f3n de Ultracongeladores de \u2212152 \u00b0C. Tasa de Fertilidad Tras Inseminaci\u00f3n Con Semen Congelado por Diferentes Protocolos de Crioconservaci\u00f3n Vector Plus. Available online: http:\/\/hdl.handle.net\/10553\/6639."},{"key":"ref_8","unstructured":"Tabarez, A. (2014). Optimizaci\u00f3n del Protocolo de Crioconservaci\u00f3n de Semen Caprino de la Raza Aut\u00f3ctona en Peligro de Extinci\u00f3n Blanca de Rasquera. [Ph.D. Thesis, Universidad Aut\u00f3noma de Barcelona]."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/s10561-008-9081-4","article-title":"Cryopreservation of domestic animal sperm cells","volume":"10","author":"Barbas","year":"2009","journal-title":"Cell Tissue Bank."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0378-4320(00)00155-X","article-title":"Storage of ram semen","volume":"62","author":"Salamon","year":"2000","journal-title":"Anim. Rep. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.smallrumres.2005.02.015","article-title":"A review on goat sperm cryopreservation","volume":"63","author":"Purdy","year":"2006","journal-title":"Small Rumin. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"291","DOI":"10.18597\/rcog.468","article-title":"Fundamentos de Crioconservaci\u00f3n","volume":"57","author":"Madero","year":"2006","journal-title":"Rev. Colomb. Obstet. Ginecol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3733","DOI":"10.1021\/jf053174e","article-title":"Hen egg yolk low-density lipoproteins film spreading at the air-water and oil-water interfaces","volume":"54","author":"Dauphas","year":"2006","journal-title":"J. Agric. Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1095\/biolreprod61.3.590","article-title":"Bovine seminal plasma phospholipid-binding proteins stimulate phospholipid efflux from epididymal sperm","volume":"61","author":"Moreau","year":"1999","journal-title":"Biol. Reprod."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1095\/biolreprod.103.022996","article-title":"Low-density lipoprotein fraction from hen\u2019s egg yolk decreases the binding of the major proteins of bovine seminal plasma to sperm and prevents lipid efflux from the sperm membrane","volume":"70","author":"Bergeron","year":"2004","journal-title":"Biol. Reprod."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1051\/rnd:19820503","article-title":"R\u00f4le du plasma s\u00e9minal dans la survie in vitro des spermatozo\u00efdes de bouc","volume":"22","author":"Nunes","year":"1982","journal-title":"Rep. Nutr. D\u00e9v."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0890-6238(95)00021-2","article-title":"The use of an in vitro sperm activation assay to detect chemically induced damage of human sperm nuclei","volume":"9","author":"Sawyer","year":"1995","journal-title":"Reprod. Toxicol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1111\/j.1439-0531.2005.00544.x","article-title":"The effect of removal of seminal plasma, egg yolk level and season on sperm freezability of canary buck (Capra hircus)","volume":"40","author":"Cabrera","year":"2005","journal-title":"Reprod. Domest. Anim."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1071\/BI9660871","article-title":"Effect of casein, lecithin, glycerol, and storage at 5 \u00b0C on diluted ram and bull semen","volume":"19","author":"Wales","year":"1966","journal-title":"Aust. J. Biol. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"856","DOI":"10.3168\/jds.S0022-0302(72)85582-6","article-title":"Non-dialyzable skim milk in diluents for ram and bull semen","volume":"55","author":"Jones","year":"1972","journal-title":"J. Dairy Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1095\/biolreprod57.5.1023","article-title":"Deterioration of goat sperm viability in milk extenders is due to a bulbourethral 60-kilodalton glycoprotein with triglyceride lipase activity","volume":"57","author":"Magallon","year":"1997","journal-title":"Biol. Reprod."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1530\/jrf.0.1120095","article-title":"Deterioration of goat spermatozoa in skimmed milk-based extenders as a result of oleic acid released by the bulbourethral lipase BUSgp60","volume":"112","author":"Combarnous","year":"1998","journal-title":"Reproduction"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"198","DOI":"10.2527\/jas1978.471198x","article-title":"Effect of Orvus Es Paste on acrosome morphology, motility and fertilizing capacity of frozen thawed boar sperm","volume":"47","author":"Pursel","year":"1978","journal-title":"J. Anim. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.3168\/jds.S0022-0302(87)80194-7","article-title":"Glycerolation and thawing effects on bull spermatozoa frozen in detergent-treated egg yolk and whole egg extenders","volume":"70","author":"Arriola","year":"1987","journal-title":"J. Dairy Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1016\/S0093-691X(00)00397-6","article-title":"Effects of Equex, one-or two-step dilution, and two freezing and thawing rates on post-thaw survival of dog spermatozoa","volume":"54","author":"Forsberg","year":"2000","journal-title":"Theriogenology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/0306-4565(76)90003-6","article-title":"The protection of ram and bull spermatozoa by the low density lipoprotein fraction of egg yolk during storage at 5 \u00b0C and deep freezing","volume":"1","author":"Watson","year":"1976","journal-title":"J. Therm. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1016\/S0093-691X(97)00066-6","article-title":"Effects of Equex STM paste on viability of frozen-thawed dog spermatozoa during in vitro incubation at 38 \u00b0C","volume":"47","author":"Rota","year":"1997","journal-title":"Theriogenology"},{"key":"ref_28","first-page":"468","article-title":"Estudio comparativo del efecto de tres diluyentes sobre la supervivencia de semen canino almacenado refrigerado a 4 \u00b0C","volume":"25","author":"Stornelli","year":"2001","journal-title":"Rev. Bras. Reprod. Anim."},{"key":"ref_29","first-page":"483","article-title":"Effects of two different temperatures and three different extenders on survival and longevity of chilled canine semen","volume":"57","author":"Stornelli","year":"2002","journal-title":"Theriogenology"},{"key":"ref_30","first-page":"457","article-title":"Freezing of boar sperm in 0.5 ml straw. Results in the field (Congelation du sperme de verrat en poillete de 0.5 mL. Resultats sur le terrain","volume":"141","author":"Thilmant","year":"1997","journal-title":"Ann. Med. Vet."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1051\/rnd:19740502","article-title":"Viabilit\u00e9 des spermatozoides de bouc conserv\u00e9s et congel\u00e9s avec ou sans leur plasma seminal: Effet du glucose","volume":"14","author":"Corteel","year":"1974","journal-title":"Ann. Biol. Anim. Biochim. Biophys."},{"key":"ref_32","unstructured":"Goat, P. (1981). Collection, Processing and Artificial Insemination of Goat Semen, Academic Press Inc."},{"key":"ref_33","unstructured":"Cabodevila, J., and Catena, M. (2020, October 16). Evaluaci\u00f3n de Semen Bovino Congelado. Available online: www.engormix.com\/ganaderia-carne\/articulos\/evaluacion-semen-bovino-congeleado-t29765.htm."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.cryobiol.2014.02.008","article-title":"In vitro assessment of soybean lecithin and egg yolk based diluents for cryopreservation of goat semen","volume":"68","author":"Salmani","year":"2014","journal-title":"Cryobiology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/0093-691X(74)90008-9","article-title":"Glutaraldehyde fixation of boar spermatozoa for acrosome evaluation","volume":"1","author":"Pursel","year":"1974","journal-title":"Theriogenology"},{"key":"ref_36","unstructured":"Hern\u00e1ndez-Corredor, L. (2021, March 03). Evaluaci\u00f3n de la Motilidad Esperm\u00e1tica de Semen Caprino Criopreservado Bajo Diferentes Medios Diluyentes a Trav\u00e9s del Sistema Casa. Available online: https:\/\/repository.unad.edu.co\/handle\/10596\/2518."},{"key":"ref_37","unstructured":"Borja-Melara, C.A. (1992). Congelaci\u00f3n de Semen Caprino en Nitr\u00f3geno L\u00edquido con Cinco Diluyentes Para uso en Inseminaci\u00f3n Artificial. [Ph.D. Thesis, University of El Salvador]. Available online: https:\/\/oatd.org\/oatd\/record?record=oai%5C%3Ari.ues.edu.sv%5C%3A17417."},{"key":"ref_38","unstructured":"Carpio-Chuchuca, S.V. (2015). Evaluaci\u00f3n de Dos Diluyentes Para la Crioconservaci\u00f3n de Semen Bovino: Yema de Huevo vs. Leche Descremada. [Bachelor\u2019s Thesis, Universidad Polit\u00e9cnica Salesiana]. Available online: http:\/\/dspace.ups.edu.ec\/handle\/123456789\/7955."},{"key":"ref_39","unstructured":"L\u00f3pez, H.A.C. (2021, February 01). Comparaci\u00f3n de Tres Diluyentes (Triladyl\u00ae, Andromed\u00ae y Leche Descremada) en la Motilidad Progresiva Post-descongelaci\u00f3n de Semen de Macho Caprino. Available online: https:\/\/www.academia.edu\/30936045\/Comparaci%C3%B3n_de_Tres_Diluyentes_Triladyl_Andromed_y_Leche_Descremada_en_la_Motilidad_Progresiva_Post_descongelaci%C3%B3n_de_Semen_de_Macho_Caprino."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.4321\/S0004-05922011000400024","article-title":"Relaci\u00f3n entre variables subjetivas e informatizadas del movimiento esperm\u00e1tico del morueco","volume":"60","author":"Bravo","year":"2011","journal-title":"Arch. Zootec."},{"key":"ref_41","unstructured":"Tom\u00e1s, C. (2007). Nuevos Protocolos Para la Crioconservaci\u00f3n de Espermatozoides de Macho Cabr\u00edo. [Master\u2019s Thesis, Universidad Polit\u00e9cnica de Valencia]."},{"key":"ref_42","first-page":"55","article-title":"Par\u00e1metros cin\u00e9ticos en eyaculados bovinos de toros de raza frisona y rubia gallega","volume":"102","year":"2006","journal-title":"ITEA"},{"key":"ref_43","unstructured":"World Health Organization (2010). WHO Laboratory Manual for the Examination and Processing of Human Semen, World Health Organization. [5th ed]."},{"key":"ref_44","first-page":"127","article-title":"Motilidad y da\u00f1o acrosomal del semen caprino congelado en pajillas de 0.25 ml y 0.5 ml y descongelado a dos diferentes ritmos de temperatura","volume":"25","author":"Valencia","year":"1994","journal-title":"Vet. M\u00e9x."},{"key":"ref_45","first-page":"116","article-title":"Effects of cryopreservation on sperm motility in white Andalusian Serrana goat","volume":"34","author":"Vallecillo","year":"2004","journal-title":"S. Afr. J. Anim. Sci."},{"key":"ref_46","unstructured":"Luzardo, A. (2010). Comparaci\u00f3n de dos Diluyentes de Base Tris (Hidroximetilaminometano) Sobre la Motilidad del Semen Caprino Congelado-Descongelado. [Licenciate Thesis, Universidad de Oriente]. Available online: http:\/\/ri2.bib.udo.edu.ve:8080\/jspui\/handle\/123456789\/2008."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.cryobiol.2013.06.001","article-title":"Optimizing conditions for treating goat semen with cholesterol-loaded cyclodextrins prior to freezing to improve cryosurvival","volume":"67","author":"Konyali","year":"2013","journal-title":"Cryobiology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/j.theriogenology.2003.12.003","article-title":"Effects of the supplementation of trehalose extender containing egg yolk with sodium dodecyl sulfate on the freezability of goat spermatozoa","volume":"62","author":"Aboagla","year":"2004","journal-title":"Theriogenology"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.anireprosci.2003.11.003","article-title":"The effect of Equex STM paste and sperm morphology on post-thaw survival of cat epididymal spermatozoa","volume":"84","author":"Hermansson","year":"2004","journal-title":"Anim. Rep. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1292\/jvms.68.1125","article-title":"Effects of addition of sodium lauryl sulfate on frozen-thawed canine spermatozoa","volume":"68","author":"Hori","year":"2006","journal-title":"J. Vet. Med. Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/2\/309\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:07:02Z","timestamp":1760119622000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/2\/309"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,16]]},"references-count":50,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,1]]}},"alternative-id":["ani13020309"],"URL":"https:\/\/doi.org\/10.3390\/ani13020309","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,16]]}}}