{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T22:23:54Z","timestamp":1775687034400,"version":"3.50.1"},"reference-count":60,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T00:00:00Z","timestamp":1674172800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deputyship for Research and Innovation, Ministry of Education, Saudi Arabia","award":["INST096"],"award-info":[{"award-number":["INST096"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study was proposed to highlight the impact of dietary Spirulina platensis (SP) supplementation in alleviating the deterioration effect of Escherichia coli (EC) on the growth performance, redox biomarkers, immune reaction, and hindgut microbial counts and acidosis in broiler chickens. Four hundred Cobb500, one-day-old, broiler chickens were deposited in battery cages (10 chicks per cage). The chicks were distributed into totally randomized 2 \u00d7 2 factorial treatments (10 replicate cages per treatment) from the day 22 to the day 42 of age. Birds of two of the groups were fed on a basal diet without SP supplementation (-SP groups), while birds of the other two groups were fed on a basal diet supplemented with 10 g\/kg SP (+SP groups). At day 36th of age, birds in one of the -SP and +SP groups were challenged by an intraperitoneal (i.p.) injection with 107 CFU\/bird EC (O157:H7 strain) in 0.5 mL sterilized saline (+EC groups), whereas the other non-challenged groups were i.p. injected with 0.5 mL saline only (-EC groups). The current study results indicated that the boilers challenged with EC had a significant (p &lt; 0.05) lower performance, poor antioxidant activity, immunosuppression, and higher numbers of pathogenic bacteria in the intestine when compared with the non-challenged birds. Dietary SP inclusion enhanced (p &lt; 0.05) broiler growth, antioxidant activity, immune response, and intestinal beneficial bacteria and acidosis. Moreover, SP alleviated the reduction in all these parameters after exposure to EC infection. Therefore, diets containing 10 g\/kg SP could be used as a promising approach to maximize broilers\u2019 production and support their health, particularly when challenged with EC infection.<\/jats:p>","DOI":"10.3390\/ani13030363","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T03:26:41Z","timestamp":1674444401000},"page":"363","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The Effects of Dietary Spirulina platensisis on Physiological Responses of Broiler Chickens Exposed to Endotoxin Stress"],"prefix":"10.3390","volume":"13","author":[{"given":"Abdulaziz A.","family":"Alaqil","sequence":"first","affiliation":[{"name":"Department of Animal and Fish Production, College of Agricultural and Food Sciences, King Faisal University, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed O.","family":"Abbas","sequence":"additional","affiliation":[{"name":"Department of Animal and Fish Production, College of Agricultural and Food Sciences, King Faisal University, Al-Ahsa 31982, Saudi Arabia"},{"name":"Department of Animal Production, Faculty of Agriculture, Cairo University, 7 Gamma St., Giza 12613, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.19080\/JOJCS.2018.07.555723","article-title":"Importance of Stress Factors in Poultry","volume":"7","author":"Elitok","year":"2018","journal-title":"Juniper Online J. Case Stud."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1986","DOI":"10.2527\/jas.2011-3949","article-title":"Acute Heat Stress Impairs Performance Parameters and Induces Mild Intestinal Enteritis in Broiler Chickens: Role of Acute Hypothalamic-Pituitary-Adrenal Axis Activation","volume":"90","author":"Rodrigues","year":"2012","journal-title":"J. Anim. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2949","DOI":"10.3382\/ps.2013-03366","article-title":"Effects of a Probiotic, Enterococcus Faecium, on Growth Performance, Intestinal Morphology, Immune Response, and Cecal Microflora in Broiler Chickens Challenged with Escherichia Coli K88","volume":"92","author":"Cao","year":"2013","journal-title":"Poult. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2589","DOI":"10.3382\/ps.2010-00904","article-title":"Efficacy of a Bacteriophage Isolated from Chickens as a Therapeutic Agent for Colibacillosis in Broiler Chickens","volume":"89","author":"Lau","year":"2010","journal-title":"Poult. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1080\/03079450802216652","article-title":"Colibacillosis in Poultry: Unravelling the Molecular Basis of Virulence of Avian Pathogenic Escherichia Coli in Their Natural Hosts","volume":"37","author":"Dziva","year":"2008","journal-title":"Avian. Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/1806-9061-2019-1101","article-title":"Effects of E. coli Infection on the Expressions of TGF-\u03b2\/Smads Signaling Pathway in Broiler Intestine","volume":"22","author":"Juang","year":"2020","journal-title":"Braz. J. Poult. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.14202\/vetworld.2021.2727-2734","article-title":"Interleukin Gene Expression in Broiler Chickens Infected by Different Escherichia Coli Serotypes","volume":"14","author":"Elnagar","year":"2021","journal-title":"Vet World"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"103861","DOI":"10.1016\/j.micpath.2019.103861","article-title":"Impact of Escherichia Coli Infection in Broiler Breeder Chicks: The Effect of Oxidative Stress on Weight Gain","volume":"139","author":"Alba","year":"2020","journal-title":"Microb. Pathog."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.vetpar.2011.03.029","article-title":"Disturbance of Oxidant\/Antioxidant Equilibrium in Horses Naturally Infected with Trypanosoma Evansi","volume":"180","author":"Ranjithkumar","year":"2011","journal-title":"Vet. Parasitol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"101629","DOI":"10.1016\/j.psj.2021.101629","article-title":"The Influence of Immune Stress Induced by Escherichia Coli Lipopolysaccharide on the Pharmacokinetics of Danofloxacin in Broilers","volume":"101","author":"Wang","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Abbas, A.O., Alaqil, A.A., El-Beltagi, H.S., Abd El-Atty, H.K., and Kamel, N.N. (2020). Modulating Laying Hens Productivity and Immune Performance in Response to Oxidative Stress Induced by E. coli Challenge Using Dietary Propolis Supplementation. Antioxidants, 9.","DOI":"10.3390\/antiox9090893"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"14176","DOI":"10.1038\/s41598-019-50511-7","article-title":"Antimicrobial Peptide, CLF36, Affects Performance and Intestinal Morphology, Microflora, Junctional Proteins, and Immune Cells in Broilers Challenged with E. coli","volume":"9","author":"Daneshmand","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_13","first-page":"1334","article-title":"Spirulina-The Nature\u2019s Wonder: A Review","volume":"2","author":"Mohan","year":"2014","journal-title":"Sch. J. Appl. Med. Sci. (SJAMS)"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1016\/j.ejmech.2012.06.025","article-title":"Synthesis, Antidepressant and Antimicrobial Activities of Some Novel Stearic Acid Analogues","volume":"54","author":"Jubie","year":"2012","journal-title":"Eur. J. Med. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2451","DOI":"10.3382\/ps\/pey093","article-title":"Effect of Dietary Spirulina (Arthrospira) Platensis on the Growth Performance, Antioxidant Enzyme Activity, Nutrient Digestibility, Cecal Microflora, Excreta Noxious Gas Emission, and Breast Meat Quality of Broiler Chickens","volume":"97","author":"Park","year":"2018","journal-title":"Poult. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"134","DOI":"10.25163\/microbbioacts.412117B0719110521","article-title":"Bioactive Properties of Spirulina: A Review","volume":"4","author":"Anvar","year":"2021","journal-title":"Microb. Bioact."},{"key":"ref_17","first-page":"7","article-title":"Efficacy of Spirulina platensis on Immune Functions in Cancer Mice with Systemic Candidiasis","volume":"1","author":"Shokri","year":"2014","journal-title":"J. Mycol. Res."},{"key":"ref_18","first-page":"133","article-title":"Determination of Some Haematological and Non-Specific Im-Mune Parameters in Nile Tilapia (Oreochromis Niloticus L., 1758) Fed with Spirulina (Spirulina platensis) Added Diets","volume":"1","year":"2015","journal-title":"J. Aquac. Eng. Fish. Res."},{"key":"ref_19","first-page":"235","article-title":"The Study of Biochemical and Histopathological Effects of Spirulina in Rats with TNBS-Induced Colitis","volume":"112","author":"Coskun","year":"2011","journal-title":"Bratisl. Lek. Listy"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"377","DOI":"10.7150\/ijbs.5.377","article-title":"Chemoprevention of Rat Liver Toxicity and Carcinogenesis by Spirulina","volume":"5","author":"Ismail","year":"2009","journal-title":"Int. J. Biol. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/j.nut.2010.04.002","article-title":"Antioxidant Activity and Hepatoprotective Effects of Whey Protein and Spirulina in Rats","volume":"27","author":"Gad","year":"2011","journal-title":"Nutrition"},{"key":"ref_22","first-page":"2650","article-title":"Dietary Administration of Spirulina platensis as Probiotics on Growth Performance and Histopathology in Broiler Chicks","volume":"6","author":"Shanmugapriya","year":"2015","journal-title":"Int. J. Recent Sci. Res."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Omar, A.E., Al-Khalaifah, H.S., Osman, A., Gouda, A., Shalaby, S.I., Roushdy, E.M., Abdo, S.A., Ali, S.A., Hassan, A.M., and Amer, S.A. (2022). Modulating the Growth, Antioxidant Activity, and Immunoexpression of Proinflammatory Cytokines and Apoptotic Proteins in Broiler Chickens by Adding Dietary Spirulina platensis Phycocyanin. Antioxidants, 11.","DOI":"10.3390\/antiox11050991"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"45578","DOI":"10.1007\/s11356-022-19115-z","article-title":"Potential Ameliorative Role of Spirulina platensis in Powdered or Extract Forms against Cyclic Heat Stress in Broiler Chickens","volume":"29","author":"Elbaz","year":"2022","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Moustafa, E.S., Alsanie, W.F., Gaber, A., Kamel, N.N., Alaqil, A.A., and Abbas, A.O. (2021). Blue-Green Algae (Spirulina platensis) Alleviates the Negative Impact of Heat Stress on Broiler Production Performance and Redox Status. Animals, 11.","DOI":"10.3390\/ani11051243"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"556","DOI":"10.5713\/ajas.17.0483","article-title":"Effects of Dietary Spirulina on Antioxidant Status, Lipid Profile, Immune Response and Performance Characteristics of Broiler Chickens Reared under High Ambient Temperature","volume":"31","author":"Mirzaie","year":"2018","journal-title":"Asian-Australas J. Anim. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1007\/s12011-021-02662-w","article-title":"Synergistic Effect of Spirulina platensis and Selenium Nanoparticles on Growth Performance, Serum Metabolites, Immune Responses, and Antioxidant Capacity of Heat-Stressed Broiler Chickens","volume":"200","author":"Shehata","year":"2022","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_28","first-page":"40","article-title":"Effect of Non-Antibiotic Feed Additives as an Alternative to Flavomycin on Productivity, Meat Quality and Blood Parameters in Broilers","volume":"75","author":"Attia","year":"2011","journal-title":"Arch. Fur. Geflugelkd."},{"key":"ref_29","unstructured":"AOAC Association of Official Analysis Chemists International (2005). Official Methods of Analysis of AOAC International, Association of Official Analysis Chemists International. [18th ed.]."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3707219","DOI":"10.1155\/2019\/3707219","article-title":"Functional Composition, Nutritional Properties, and Biological Activities of Moroccan Spirulina Microalga","volume":"2019","author":"Seghiri","year":"2019","journal-title":"J. Food Qual."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.toxrep.2015.02.007","article-title":"Free Radicals Quenching Potential, Protective Properties against Oxidative Mediated Ion Toxicity and HPLC Phenolic Profile of a Cameroonian Spice: Piper Guineensis","volume":"2","author":"Njimou","year":"2015","journal-title":"Toxicol. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"523","DOI":"10.14202\/vetworld.2021.523-536","article-title":"Preventive Antimicrobial Action and Tissue Architecture Ameliorations of Bacillus Subtilis in Challenged Broilers","volume":"14","author":"Soliman","year":"2021","journal-title":"Vet. World"},{"key":"ref_33","unstructured":"(2022, June 07). Cobb500 Broiler Performance & Nutrition Supplement. Available online: https:\/\/www.cobb-vantress.com\/en_US\/products\/cobb500\/."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Mehaisen, G.M.K., Eshak, M.G., el Sabry, M.I., and Abass, A.O. (2016). Expression of Inflammatory and Cell Death Program Genes and Comet DNA Damage Assay Induced by Escherichia Coli in Layer Hens. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0158314"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"103787","DOI":"10.1016\/j.micpath.2019.103787","article-title":"Impacts of Escherichia Coli Infection in Young Breeder Chicks on the Animal Behavior and Cerebral Activity of Purinergic and Cholinergic Enzymes Involved in the Regulation of Molecules with Neurotransmitter and Neuromodulator Function","volume":"138","author":"Alba","year":"2020","journal-title":"Microb. Pathog."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1080\/1745039X.2011.609753","article-title":"Effect of Copper on Antioxidant Ability and Nutrient Metabolism in Broiler Chickens Stimulated by Lipopolysaccharides","volume":"65","author":"Song","year":"2011","journal-title":"Arch. Anim. Nutr."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"193","DOI":"10.3923\/ijps.2008.193.201","article-title":"The Role of Light Program and Melatonin on Alleviation of Inflammation Induced by Lipopolysaccharide Injection in Broiler Chickens","volume":"7","author":"Gehad","year":"2008","journal-title":"Int. J. Poult. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2033","DOI":"10.3382\/ps.2009-00128","article-title":"Transport Stress in Broilers: I. Blood Metabolism, Glycolytic Potential, and Meat Quality","volume":"88","author":"Zhang","year":"2009","journal-title":"Poult. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Alaqil, A.A., Abbas, A.O., El-Beltagi, H.S., Abd El-Atty, H.K., Mehaisen, G.M.K., and Moustafa, E.S. (2020). Dietary Supplementation of Probiotic Lactobacillus Acidophilus Modulates Cholesterol Levels, Immune Response, and Productive Performance of Laying Hens. Animals, 10.","DOI":"10.3390\/ani10091588"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1080\/1547691X.2016.1264503","article-title":"Protective Role of Bentonite against Aflatoxin B1- and Ochratoxin A-Induced Immunotoxicity in Broilers","volume":"14","author":"Bhatti","year":"2017","journal-title":"J. Immunotoxicol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"928235","DOI":"10.3389\/fvets.2022.928235","article-title":"Effects From Dietary Addition of Sargassum Sp., Spirulina Sp., or Gracilaria Sp. Powder on Immune Status in Broiler Chickens","volume":"9","author":"Surrayai","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_42","unstructured":"(2022, October 10). IBM SPSS Statistics 22. Available online: https:\/\/www.ibm.com\/support\/pages\/downloading-ibm-spss-statistics-22."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.bbi.2007.06.002","article-title":"Inhibitory Effects of Lipopolysaccharide on Hypothalamic Nuclei Implicated in the Control of Food Intake","volume":"22","author":"Becskei","year":"2008","journal-title":"Brain Behav. Immun."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"905","DOI":"10.3923\/ijps.2006.905.911","article-title":"Factors That Affect Feed Intake of Meat Birds: A Review","volume":"5","author":"Ferket","year":"2006","journal-title":"Int. J. Poult. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1590\/S1516-35982004000600014","article-title":"Uso de Antibi\u00f3tico, de Probi\u00f3tico e de Homeopatia Em Frangos de Corte Criados Em Ambiente de Conforto, Inoculados Ou N\u00e3o Com Escherichia Coli","volume":"33","author":"Boratto","year":"2004","journal-title":"Rev. Bras. Zootec."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Surai, P.F., Kochish, I.I., Fisinin, V.I., and Kidd, M.T. (2019). Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update. Antioxidants, 8.","DOI":"10.3390\/antiox8070235"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Al-Otaibi, M.I.M., Abdellatif, H.A.M., Al-Huwail, A.K.A., Abbas, A.O., Mehaisen, G.M.K., and Moustafa, E.S. (2022). Hypocholesterolemic, Antioxidative, and Anti-Inflammatory Effects of Dietary Spirulina platensisis Supplementation on Laying Hens Exposed to Cyclic Heat Stress. Animals, 12.","DOI":"10.3390\/ani12202759"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1007\/s00204-016-1744-5","article-title":"The Antioxidant, Immunomodulatory, and Anti-Inflammatory Activities of Spirulina: An Overview","volume":"90","author":"Wu","year":"2016","journal-title":"Arch. Toxicol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1111\/asj.12729","article-title":"Influence of Procyanidin Supplementation on the Immune Responses of Broilers Challenged with Lipopolysaccharide","volume":"88","author":"Wu","year":"2017","journal-title":"Anim. Sci. J."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1016\/j.freeradbiomed.2011.06.012","article-title":"An Integrated Approach to Assessing Nitroso-Redox Balance in Systemic Inflammation","volume":"51","author":"Dyson","year":"2011","journal-title":"Free. Radic. Biol. Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/1297-9716-45-19","article-title":"Analysis of Immune Responses Induced by Avian Pathogenic Escherichia Coli Infection in Turkeys and Their Association with Resistance to Homologous Re-Challenge","volume":"45","author":"Sadeyen","year":"2014","journal-title":"Vet. Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/s13567-014-0132-5","article-title":"Immune Responses Associated with Homologous Protection Conferred by Commercial Vaccines for Control of Avian Pathogenic Escherichia Coli in Turkeys","volume":"46","author":"Sadeyen","year":"2015","journal-title":"Vet. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3247528","DOI":"10.1155\/2017\/3247528","article-title":"Antioxidant, Immunomodulating, and Microbial-Modulating Activities of the Sustainable and Ecofriendly Spirulina","volume":"2017","author":"Finamore","year":"2017","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"156","DOI":"10.21608\/jcvr.2022.240919","article-title":"Enhancement Effect of Spirulina platensis Extract on Broiler Chicks\u2019 Growth Performance and Immunity","volume":"4","author":"Elshabrawy","year":"2022","journal-title":"J. Curr. Vet. Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1017\/S000711450769936X","article-title":"Amino Acids and Immune Function","volume":"98","author":"Li","year":"2007","journal-title":"Br. J. Nutr."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"175","DOI":"10.4067\/S0719-81322017000300175","article-title":"Immune Response in Eimeria Sp. and E. coli Challenged Broilers Supplemented with Amino Acids","volume":"49","author":"Gottardo","year":"2017","journal-title":"Austral J. Vet. Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1016\/j.sjbs.2021.09.046","article-title":"Antioxidant and Antimicrobial Activities of Spirulina platensis Extracts and Biogenic Selenium Nanoparticles against Selected Pathogenic Bacteria and Fungi","volume":"29","author":"Shehata","year":"2022","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1007\/s12602-019-09613-x","article-title":"Effect of in Ovo Inoculation of Bifidobacterium Spp. on Growth Performance, Thyroid Activity, Ileum Histomorphometry, and Microbial Enumeration of Broilers","volume":"12","author":"Elbaz","year":"2020","journal-title":"Probiotics Antimicrob. Proteins"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.psj.2020.04.019","article-title":"Growth, Carcass Characteristics, Meat Quality, and Microbial Aspects of Growing Quail Fed Diets Enriched with Two Different Types of Probiotics (Bacillus Toyonensis and Bifidobacterium Bifidum)","volume":"100","author":"Elsadek","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.biortech.2017.04.005","article-title":"Rapid Generation of Volatile Fatty Acids (VFA) through Anaerobic Acidification of Livestock Organic Waste at Low Hydraulic Residence Time (HRT)","volume":"238","author":"Kuruti","year":"2017","journal-title":"Bioresour. Technol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/3\/363\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:12:09Z","timestamp":1760119929000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/3\/363"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,20]]},"references-count":60,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["ani13030363"],"URL":"https:\/\/doi.org\/10.3390\/ani13030363","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,20]]}}}