{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:49:35Z","timestamp":1761061775363,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,1,26]],"date-time":"2023-01-26T00:00:00Z","timestamp":1674691200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Brazilian National Council of Scientific and Technological Development (CNPq; Brazil)"},{"name":"Coordination for the Improvement of Higher Education Personnel (CAPES; Brazil)"},{"name":"National Institute of Science and Technology of Dairy Production Chain (INCT-Leite)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Bovine gammaherpesvirus 6 (BoGHV6), previously known as bovine lymphotropic virus, is a member of the Macavirus genus, subfamily Gammaherpesvirinae. Other members of the genus Macavirus include viruses that produce malignant catarrhal fever (MCF) in mammalian hosts, collectively referred to as the MCF virus (MCFV) complex, and the porcine lymphotropic herpesvirus (PLHV). However, the current role of BoGHV6 in the development of diseases and\/or disease syndromes remains uncertain and controversial. This paper investigated the participation of BoGHV6 in the development of pulmonary disease in a cow with interstitial pneumonia by histopathology and molecular testing. Tissue antigens of common viral agents of respiratory diseases and Mycoplasma bovis were not identified by immunohistochemistry. Additionally, molecular assays designed to amplify common bacterial and viral pathogens of pulmonary disease did not amplify the nucleic acids of these agents. However, a pan-PCR assay amplified the DNA of the herpesvirus polymerase gene, while the specific BoGHV6 nested-PCR assay amplified the partial fragment of the BoGHV6 polymerase gene derived from the pulmonary tissue with interstitial pneumonia. Phylogenetic analysis revealed that the BoGHV6 strain herein identified had 99.8% nucleotide (nt) sequence identity with reference strains of BoGHV6, but only 72.2\u201373.5% and 67.9\u201368.6% nt identity with reference strains of MCFV and PLHV, respectively. Consequently, these results suggest that BoGHV6 was associated with the pulmonary disease observed in this cow.<\/jats:p>","DOI":"10.3390\/ani13030417","type":"journal-article","created":{"date-parts":[[2023,1,27]],"date-time":"2023-01-27T02:30:38Z","timestamp":1674786638000},"page":"417","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Possible Association of Bovine Gammaherpesvirus 6 with Pulmonary Disease in a Cow"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1614-0185","authenticated-orcid":false,"given":"Selwyn Arlington","family":"Headley","sequence":"first","affiliation":[{"name":"Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Paran\u00e1, Brazil"},{"name":"Multi-User Animal Health Laboratory, Tissue Processing Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Paran\u00e1, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Sa\u00fade e Produ\u00e7\u00e3o, Animal, Universidade Pit\u00e1goras-Universidade Norte do Paran\u00e1, Arapongas 86700-020, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alais Maria","family":"Dall Agnol","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Virology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Paran\u00e1, Brazil"},{"name":"Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thalita Evani Silva","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vin\u00edcius Rodrigues","family":"Bon","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Virology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela Sanches","family":"Scuisato","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Aparecida Correa","family":"Xavier","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4061-327X","authenticated-orcid":false,"given":"Carolina Yuka","family":"Yasumitsu","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Virology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice Fernandes","family":"Alfieri","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Virology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Paran\u00e1, Brazil"},{"name":"Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7024-4487","authenticated-orcid":false,"given":"Amauri Alcindo","family":"Alfieri","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Virology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Paran\u00e1, Brazil"},{"name":"Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/BF01241154","article-title":"Isolation of a herpes-like virus from lymphosarcomatous cattle","volume":"37","author":"Boothe","year":"1972","journal-title":"Arch. 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