{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T22:05:15Z","timestamp":1790028315067,"version":"4.0.1"},"reference-count":55,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Research, Technology, and Higher Education of Indonesia","award":["1421\/D3\/PG\/2018"],"award-info":[{"award-number":["1421\/D3\/PG\/2018"]}]},{"name":"Ministry of Research, Technology, and Higher Education of Indonesia","award":["1345\/D3\/PG\/2018"],"award-info":[{"award-number":["1345\/D3\/PG\/2018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The purpose of this study was to identify seminal plasma proteins in Bali bull and their potential as biomarkers of fertility. Semen was collected from 10 bulls aged 5\u201310 years using an artificial vagina. Fresh semen was then centrifuged (3000\u00d7 g for 30 min). The supernatant was put into straws and stored in liquid nitrogen. The semen plasma protein concentration was determined using the Bradford method, and the protein was characterized using 1D-SDS-PAGE. Coomassie Brilliant Blue (CBB) was used to color the gel, and the molecular weight of the protein was determined using PM2700. A total of 94 proteins were identified in the seminal plasma of Bali bulls analyzed using LC-MS\/MS (liquid chromatography\u2013mass spectrometry). Proteins spermadhesin 1 (SPADH1), C-type natriuretic peptide (NPPC), clusterin (CLU), apoliprotein A-II (APOA2), inositol-3-phosphate synthase 1 (ISYNA1), and sulfhydryl oxidase 1 (QSOX1) were identified as important for fertility in Bos javanicus. These proteins may prove to be important biomarkers of fertility in Bali bulls. These proteins are important for reproductive function, which includes spermatozoa motility, capacitation, and acrosome reactions. This study provides new information about the protein content in seminal plasma in Bali bulls. The LC-MS\/MS-based proteome approach that we applied in this study obtained 94 proteins. The identification of these seminal plasma proteins of Bali bulls and their potential as fertility biomarkers may have an impact on the success of future artificial insemination (AI).<\/jats:p>","DOI":"10.3390\/ani13030514","type":"journal-article","created":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T03:24:49Z","timestamp":1675308289000},"page":"514","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Protein Identification of Seminal Plasma in Bali Bull (Bos javanicus)"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5786-5094","authenticated-orcid":false,"given":"Hikmayani","family":"Iskandar","sequence":"first","affiliation":[{"name":"Agricultural Science Study Program, Graduate School Hasanuddin University, Makassar 90245, Indonesia"},{"name":"Animal Repronomics Research Group, Research Center for Applied Zoology, National Research and Innovation Agency, Bogor 16914, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5131-3144","authenticated-orcid":false,"given":"G\u00f6ran","family":"Andersson","sequence":"additional","affiliation":[{"name":"Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, 75007 Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8545-4110","authenticated-orcid":false,"given":"Herry","family":"Sonjaya","sequence":"additional","affiliation":[{"name":"Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Makassar 90245, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7478-9433","authenticated-orcid":false,"given":"Raden","family":"Arifiantini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine, IPB University, Bogor 16680, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syahruddin","family":"Said","sequence":"additional","affiliation":[{"name":"Animal Repronomics Research Group, Research Center for Applied Zoology, National Research and Innovation Agency, Bogor 16914, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6069-2171","authenticated-orcid":false,"given":"Hasbi","family":"Hasbi","sequence":"additional","affiliation":[{"name":"Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Makassar 90245, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tulus","family":"Maulana","sequence":"additional","affiliation":[{"name":"Animal Repronomics Research Group, Research Center for Applied Zoology, National Research and Innovation Agency, Bogor 16914, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3688-8844","authenticated-orcid":false,"given":"Abdullah","family":"Baharun","sequence":"additional","affiliation":[{"name":"Animal Science Program, Faculty of Agriculture, Djuanda University, Bogor 16720, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1111\/j.1439-0531.2011.01958.x","article-title":"Indigenous Bali Cattle Is Most Suitable for Sustainable Small Farming in Indonesia","volume":"47","author":"Martojo","year":"2012","journal-title":"Reprod. 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