{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T09:35:04Z","timestamp":1780392904034,"version":"3.54.1"},"reference-count":42,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Guangdong Province Modern Agriculture Poultry Industry technology system innovation team construction project","award":["2022KJ128"],"award-info":[{"award-number":["2022KJ128"]}]},{"name":"Guangdong Province Modern Agriculture Poultry Industry technology system innovation team construction project","award":["DZX20192520309"],"award-info":[{"award-number":["DZX20192520309"]}]},{"name":"Guangdong Province Modern Agriculture Poultry Industry technology system innovation team construction project","award":["CGZ0400162"],"award-info":[{"award-number":["CGZ0400162"]}]},{"name":"Guangdong Science and Technology Innovation Strategy Special Fund","award":["2022KJ128"],"award-info":[{"award-number":["2022KJ128"]}]},{"name":"Guangdong Science and Technology Innovation Strategy Special Fund","award":["DZX20192520309"],"award-info":[{"award-number":["DZX20192520309"]}]},{"name":"Guangdong Science and Technology Innovation Strategy Special Fund","award":["CGZ0400162"],"award-info":[{"award-number":["CGZ0400162"]}]},{"name":"Discipline Construction Program of Foshan University","award":["2022KJ128"],"award-info":[{"award-number":["2022KJ128"]}]},{"name":"Discipline Construction Program of Foshan University","award":["DZX20192520309"],"award-info":[{"award-number":["DZX20192520309"]}]},{"name":"Discipline Construction Program of Foshan University","award":["CGZ0400162"],"award-info":[{"award-number":["CGZ0400162"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This experiment investigated the benefits of plant essential oil (EO) composed with lauric acid monoglyceride and cinnamaldehyde on necrotic enteritis-challenged broilers. A total of 180 1-day-old healthy yellow-feathered broilers were randomly divided into 4 groups with 3 replicates of 15 chicks each. The experimental groups were as follows: the control group (CON) was fed with the basal diet and was not challenged by Eimeria acervulina (EA) and Clostridium perfringens (CP); CPEA group was also fed with a basal diet, but infected with CP and EA; CPEA_EO350 group and CPEA_EO500 group were fed with a basal diet supplemented with 350 and 500 mg\/kg EO, respectively, and all infected with CP and EA. On the 7th day, each bird in the CPEA group, CPEA_EO350 group and CPEA_EO500 group was orally administrated with 1 mL Eimeria acervulina containing 5000 oocytes\/mL, and the birds of the CON group were orally administrated with 1 mL normal saline. From the 15th day, 1 mL of CP type A CVCC-2030 strain (about 5 \u00d7 108 cfu\/mL) was orally inoculated into each bird of the CPEA, CPEA_EO350 and CPEA_EO500 groups for three consecutive days. Similarly, the CON group was orally given 1 mL of normal saline. The CPEA stimulation reduced the average daily gain (ADG) of broilers, increased the feed-to-gain ratio (F:G), and increased the intestinal lesions of the broilers (p &lt; 0.01), indicating that CPEA stimulation was clinically successful. Compared with the CPEA group, the ADG of CPEA_EO350 and CPEA_EO500 increased, the F:G decreased (p &lt; 0.01), and the intestinal score of CPEA_EO500 decreased (p &lt; 0.01). The expression of the tight junction protein of the jejunum and ileum on 21d was upregulated (p &lt; 0.01), and the expression of jejunum inflammation factors TNF-\u03b1 on 21d and jejunum and ileum inflammatory factor IL-6 on 28d were also downregulated. The CPEA_EO350 and CPEA_EO500 increased antioxidant capacity. To sum up, 350 and 500 mg\/kg of lauric acid monoglyceride and cinnamaldehyde complex plant essential oils can improve ADG and F:G, improve intestinal morphology and the body\u2019s antioxidant capacity, and downregulate the expression of inflammatory factors. The concentration of 500 mg\/kg performed even better.<\/jats:p>","DOI":"10.3390\/ani13030516","type":"journal-article","created":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T03:24:49Z","timestamp":1675308289000},"page":"516","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Complex of Lauric Acid Monoglyceride and Cinnamaldehyde Ameliorated Subclinical Necrotic Enteritis in Yellow-Feathered Broilers by Regulating Gut Morphology, Barrier, Inflammation and Serum Biochemistry"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0067-0867","authenticated-orcid":false,"given":"Chaojun","family":"Zheng","sequence":"first","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9444-1223","authenticated-orcid":false,"given":"Gengsheng","family":"Xiao","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Yan","sequence":"additional","affiliation":[{"name":"Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Qiu","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiancun","family":"Ou","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingzhu","family":"Cen","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Gong","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiansheng","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Life Science and Food Engineering, Hebei University of Engineering, Handan 056038, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihua","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1080\/03079457.2016.1163521","article-title":"Epidemiologic aspects of necrotic enteritis in broiler chickens\u2014Disease occurrence and production performance","volume":"45","author":"Kaldhusdal","year":"2016","journal-title":"Avian Pathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e26","DOI":"10.1371\/journal.ppat.0040026","article-title":"NetB, a new toxin that is associated with avian necrotic enteritis caused by Clostridium perfringens","volume":"4","author":"Keyburn","year":"2008","journal-title":"PLoS Pathog."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1080\/03079457.2016.1150587","article-title":"Necrotic enteritis predisposing factors in broiler chickens","volume":"45","author":"Moore","year":"2016","journal-title":"Avian Pathol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2839","DOI":"10.1021\/jf00059a013","article-title":"Antibacterial activity of some essential oil components against five foodborne pathogens","volume":"43","author":"Kim","year":"1995","journal-title":"J. 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