{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T15:39:28Z","timestamp":1774539568174,"version":"3.50.1"},"reference-count":43,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Korean government (MSIT)","award":["2019K2A9A1A09081549"],"award-info":[{"award-number":["2019K2A9A1A09081549"]}]},{"name":"Korean government (MSIT)","award":["MAFRA; 716002-7"],"award-info":[{"award-number":["MAFRA; 716002-7"]}]},{"name":"Korean government (MSIT)","award":["RSPD2023R725"],"award-info":[{"award-number":["RSPD2023R725"]}]},{"name":"Korea Institute of Planning and Evaluation for Technology (IPET) in Food, Agriculture, and Forestry","award":["2019K2A9A1A09081549"],"award-info":[{"award-number":["2019K2A9A1A09081549"]}]},{"name":"Korea Institute of Planning and Evaluation for Technology (IPET) in Food, Agriculture, and Forestry","award":["MAFRA; 716002-7"],"award-info":[{"award-number":["MAFRA; 716002-7"]}]},{"name":"Korea Institute of Planning and Evaluation for Technology (IPET) in Food, Agriculture, and Forestry","award":["RSPD2023R725"],"award-info":[{"award-number":["RSPD2023R725"]}]},{"name":"King Saud University","award":["2019K2A9A1A09081549"],"award-info":[{"award-number":["2019K2A9A1A09081549"]}]},{"name":"King Saud University","award":["MAFRA; 716002-7"],"award-info":[{"award-number":["MAFRA; 716002-7"]}]},{"name":"King Saud University","award":["RSPD2023R725"],"award-info":[{"award-number":["RSPD2023R725"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study aimed to investigate the effects of cold drinking water on cellular and humoral immunity in heat-exposed laying hens. One hundred and eight laying hens at 19 weeks old were placed into three treatments with six replicates of six hens in each group as follows: (1) hens were provided with normal drinking water (NW) under the control of thermoneutral temperature (CT: 25 \u00b1 1 \u00b0C; CT + NW), (2) hens were provided with NW under high ambient temperature (HT: 35 \u00b1 1 \u00b0C; HT + NW) for 8 h\/d for a month, and (3) hens were treated under HT with cold drinking water (CW: 15 \u00b1 1 \u00b0C; HT + CW) for 8 h\/d for a 4-weeks. Then, the feed consumption, egg production, egg weight, feed conversion ratio, and blood immune parameters were investigated. The results showed that cold drinking water (CW) caused a significant (p &lt; 0.05) recovery in the reduction of food intake and egg production due to heat stress; however, there was no significant effect (p &gt; 0.05) on egg weight and feed conversion ratio. Moreover, CW significantly (p &lt; 0.05) restored the immune-suppressing effects of heat stress on the contents of peripheral blood mononuclear cells, including B-cell (BU-Ia), helper T cell (CD4), and the ratio of helper\/cytotoxic T cell (CD4\/CD8). In addition, CW significantly (p &lt; 0.05) recovered the reduction on the level of mRNA expression of interleukin-2 (IL-2) and interferon-gamma (IFN-\u03b3), as well as significantly (p &lt; 0.05) restored the reduction of plasma concentration of IL-2, IFN-\u03b3 and immunoglobulin G in heat-stressed laying hens. These results prove that CW increased heat dissipation and enhanced feed intake, egg production, and cellular and humoral immunity in heat-exposed laying hens.<\/jats:p>","DOI":"10.3390\/ani13040580","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T02:28:17Z","timestamp":1675736897000},"page":"580","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Cold Drinking Water Boosts the Cellular and Humoral Immunity in Heat-Exposed Laying Hens"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2941-6328","authenticated-orcid":false,"given":"Hatem M.","family":"Eltahan","sequence":"first","affiliation":[{"name":"Animal Production Research Institute, Agriculture Research Center, Agriculture Ministry, Sakha, Kafr El-Sheikh 33717, Egypt"},{"name":"Postdoc at the Department of Animal Science, Jeonbuk National University, Jeonju 54896, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang W.","family":"Kang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jeonbuk National University, Jeonju 54596, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vishwajit S.","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Division for Experimental Natural Science, Faculty of Arts and Science, Graduate School of Bioresource and Bioenvironmental Science, Kyushu University, Fukuoka 819-0395, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5511-7546","authenticated-orcid":false,"given":"Hossam M.","family":"Eltahan","sequence":"additional","affiliation":[{"name":"Animal Production Research Institute, Agriculture Research Center, Agriculture Ministry, Sakha, Kafr El-Sheikh 33717, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mostafa A.","family":"Abdel-Maksoud","sequence":"additional","affiliation":[{"name":"Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4948-5574","authenticated-orcid":false,"given":"Ayman","family":"Mubarak","sequence":"additional","affiliation":[{"name":"Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun Ik","family":"Lim","sequence":"additional","affiliation":[{"name":"Poultry Research Institute, National Institute of Animal Science, RDA, Pyeongchang 25342, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1093\/ps\/76.2.289","article-title":"Effect of the genetic selection of turkeys for increased body weight and egg production on immune and physiological responses","volume":"76","author":"Bayyari","year":"1997","journal-title":"Poult. 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