{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T22:50:34Z","timestamp":1792191034812,"version":"4.4.0"},"reference-count":30,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T00:00:00Z","timestamp":1676332800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32102561"],"award-info":[{"award-number":["32102561"]}]},{"name":"National Natural Science Foundation of China","award":["S202110504033"],"award-info":[{"award-number":["S202110504033"]}]},{"name":"National Natural Science Foundation of China","award":["202210504008"],"award-info":[{"award-number":["202210504008"]}]},{"name":"National Natural Science Foundation of China","award":["2021DG700024-KF202214"],"award-info":[{"award-number":["2021DG700024-KF202214"]}]},{"name":"the Student Research Funds of Huazhong Agricultural University","award":["32102561"],"award-info":[{"award-number":["32102561"]}]},{"name":"the Student Research Funds of Huazhong Agricultural University","award":["S202110504033"],"award-info":[{"award-number":["S202110504033"]}]},{"name":"the Student Research Funds of Huazhong Agricultural University","award":["202210504008"],"award-info":[{"award-number":["202210504008"]}]},{"name":"the Student Research Funds of Huazhong Agricultural University","award":["2021DG700024-KF202214"],"award-info":[{"award-number":["2021DG700024-KF202214"]}]},{"name":"the National Innovation and Entrepreneurship Training Program for Undergraduate","award":["32102561"],"award-info":[{"award-number":["32102561"]}]},{"name":"the National Innovation and Entrepreneurship Training Program for Undergraduate","award":["S202110504033"],"award-info":[{"award-number":["S202110504033"]}]},{"name":"the National Innovation and Entrepreneurship Training Program for Undergraduate","award":["202210504008"],"award-info":[{"award-number":["202210504008"]}]},{"name":"the National Innovation and Entrepreneurship Training Program for Undergraduate","award":["2021DG700024-KF202214"],"award-info":[{"award-number":["2021DG700024-KF202214"]}]},{"name":"the State Key Laboratory for Managing Biotic and Chemical Threats","award":["32102561"],"award-info":[{"award-number":["32102561"]}]},{"name":"the State Key Laboratory for Managing Biotic and Chemical Threats","award":["S202110504033"],"award-info":[{"award-number":["S202110504033"]}]},{"name":"the State Key Laboratory for Managing Biotic and Chemical Threats","award":["202210504008"],"award-info":[{"award-number":["202210504008"]}]},{"name":"the State Key Laboratory for Managing Biotic and Chemical Threats","award":["2021DG700024-KF202214"],"award-info":[{"award-number":["2021DG700024-KF202214"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study aimed at investigating the effects of phytosterols on the productive performance, egg quality, length of small intestine, and tibia quality in aged laying hens. A total of 960 Dawu Jinfeng commercial laying hens (75 weeks of age) were randomly assigned to three groups. Each group had 16 replicates and every replicate contained four cages (five birds\/cage). The control group hens received the basal diet without phytosterols. The hens in the experimental groups received a diet containing phytosterols at concentrations of 20 mg\/kg and 40 mg\/kg for 7 weeks. The results showed that phytosterols had a linearly increasing effect on egg weight, eggshell surface area, albumen height, and haugh unit at week 5 of experiment (p &lt; 0.05). Supplemental phytosterols linearly and quadratically increased eggshell thickness (p &lt; 0.05). At week 7 of the experiment, dietary supplementation of phytosterols linearly increased egg weight and eggshell weight (p &lt; 0.05). Supplementation of 20 mg\/kg, but not 40 mg\/kg, phytosterols increased the length of the small intestine. However, dietary phytosterols had no effect on the laying rate, mortality, or liver index (p &gt; 0.1). The results of tibia quality detected by micro-CT also showed no difference in the treatment of phytosterols. Therefore, supplementation with 20 mg\/kg phytosterols in the diet improves egg quality and increases the length of small intestine, but has no effects on the quality of the tibia.<\/jats:p>","DOI":"10.3390\/ani13040662","type":"journal-article","created":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T03:37:24Z","timestamp":1676432244000},"page":"662","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effects of Dietary Phytosterol Supplementation on the Productive Performance, Egg Quality, Length of Small Intestine, and Tibia Quality in Aged Laying Hens"],"prefix":"10.3390","volume":"13","author":[{"given":"Xiangyu","family":"Xiao","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yucheng","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bohua","family":"Deng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaojiao","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyi","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoshuai","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqing","family":"Han","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0969-3689","authenticated-orcid":false,"given":"Tongxing","family":"Song","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.2141\/jpsa.41.161","article-title":"Factors affecting egg internal quality and egg shell quality in laying hens","volume":"41","author":"Roberts","year":"2004","journal-title":"J. Poult. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.3382\/ps\/pev102","article-title":"Efficacy of various protein-based coating on enhancing the shelf life of fresh eggs during storage","volume":"94","author":"Caner","year":"2015","journal-title":"Poult. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6187","DOI":"10.3382\/ps\/pez401","article-title":"Changes in the quality of eggs during storage depending on the housing system and the age of hens","volume":"98","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4838","DOI":"10.3382\/ps\/pez219","article-title":"Effects of Lonicera confusa and Astragali Radix extracts supplementation on egg production performance, egg quality, sensory evaluation, and antioxidative parameters of laying hens during the late laying period","volume":"98","author":"Xie","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1788","DOI":"10.1111\/jpn.13423","article-title":"Alpha-lipoic acid improves the reproduction performance of breeder hens during the late egg-laying period","volume":"104","author":"Dai","year":"2020","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_6","first-page":"372","article-title":"Factors affecting quality and quantity of egg production in laying hens: A review","volume":"12","author":"Ahmadi","year":"2011","journal-title":"World Appl. Sci. J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1080\/00071668.2013.799758","article-title":"Effect of quercetin on performance and egg quality during the late laying period of hens","volume":"54","author":"Liu","year":"2013","journal-title":"Br. Poult. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1080\/00071669889024","article-title":"Bone structure and strength at different ages in laying hens and effects of dietary particulate limestone, vitamin K and ascorbic acid","volume":"39","author":"Fleming","year":"1998","journal-title":"Br. Poult. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.rvsc.2021.04.009","article-title":"Potential role of important nutraceuticals in poultry performance and health-A comprehensive review","volume":"137","author":"Alagawany","year":"2021","journal-title":"Res. Vet. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1006\/jfca.2001.1044","article-title":"Free and Esterified Sterol Composition of Edible Oils and Fats","volume":"15","author":"Katherine","year":"2002","journal-title":"J. Food Compos. Anal."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"277","DOI":"10.3177\/jnsv.49.277","article-title":"Antioxidant Effects of Phytosterol and Its Components","volume":"49","author":"Yoshida","year":"2003","journal-title":"J. Nutr. Sci. Vitaminol."},{"key":"ref_12","first-page":"67","article-title":"Physiological function of phytosterol and its application","volume":"7","author":"Xinmei","year":"2015","journal-title":"Anim. Husb. Feed. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1051\/ocl.2007.0145","article-title":"Phytosterols: Natural compounds with established and emerging health benefits","volume":"14","author":"Trautwein","year":"2007","journal-title":"Ol\u00e9agineux Corps Gras Lipides"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"101096","DOI":"10.1016\/j.psj.2021.101096","article-title":"Effects of phytosterol supplementation on growth performance, serum lipid, proinflammatory cytokines, intestinal morphology, and meat quality of white feather broilers","volume":"100","author":"Ding","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1016\/j.psj.2019.10.025","article-title":"Dietary \u03b2-sitosterol regulates serum lipid level and improves immune function, antioxidant status, and intestinal morphology in broilers","volume":"99","author":"Cheng","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"270","DOI":"10.3382\/ps.2009-00249","article-title":"Effect of plant sterol-enriched diets on plasma and egg yolk cholesterol concentrations and cholesterol metabolism in laying hens","volume":"89","author":"Liu","year":"2010","journal-title":"Poult. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"152","DOI":"10.3382\/ps.2008-00271","article-title":"Feeding laying hens a bioavailable soy sterol mixture fails to enrich their eggs with phytosterols or elicit egg yolk compositional changes","volume":"88","author":"Elkin","year":"2009","journal-title":"Poult. Sci."},{"key":"ref_18","first-page":"99","article-title":"Effects of dietary phytosterol concentration on performance, egg quality and cholesterol content in laying hens Einfluss einer hohen","volume":"79","author":"Shen","year":"2015","journal-title":"Eur. Poult. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1071\/AN12345","article-title":"Egg quality and age of laying hens: Implications for product safety","volume":"53","author":"Roberts","year":"2013","journal-title":"Anim. Prod. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1590\/S1516-635X2005000200001","article-title":"Research on eggshell structure and quality: An historical overview","volume":"7","author":"Hunton","year":"2005","journal-title":"Braz. J. Poult. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1093\/ps\/84.4.522","article-title":"Comparisons of molting diets on skeletal quality and eggshell parameters in hens at the end of the second egg-laying cycle","volume":"84","author":"Kim","year":"2005","journal-title":"Poult. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1093\/ps\/83.2.193","article-title":"Overview of bone biology in the egg-laying hen","volume":"83","author":"Whitehead","year":"2004","journal-title":"Poult. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1093\/jn\/84.1.20","article-title":"Calcium Metabolism and Skeletal Dynamics of Laying Pullets","volume":"84","author":"Mueller","year":"1964","journal-title":"J. Nutr."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3137","DOI":"10.1093\/jn\/134.11.3137","article-title":"Vitamin D and the Dual Processes of Intestinal Calcium Absorption","volume":"134","author":"Wasserman","year":"2004","journal-title":"J. Nutr."},{"key":"ref_25","unstructured":"Council, N.R. (1994). Nutrient Requirements of Poultry: Ninth Revised Edition, 1994, The National Academies Press."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"101053","DOI":"10.1016\/j.psj.2021.101053","article-title":"Comparing various euthanasia devices and methods on 8 and 12-week-old turkey hens","volume":"100","author":"Stiewert","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1080\/1828051X.2017.1344935","article-title":"Relationship between eggshell thickness and other eggshell measurements in eggs from litter and cages","volume":"17","author":"Ketta","year":"2017","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"545","DOI":"10.3382\/ps.2013-03562","article-title":"Safety evaluation of phytosterols in laying hens: Effects on laying performance, clinical blood parameters, and organ development","volume":"93","author":"Shi","year":"2014","journal-title":"Poult. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1016\/j.numecd.2014.04.013","article-title":"Effect of \u03b2-cryptoxanthin plus phytosterols on cardiovascular risk and bone turnover markers in post-menopausal women: A randomized crossover trial","volume":"24","author":"Lagarda","year":"2014","journal-title":"Nutr. Metab. Cardiovasc. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.cvex.2007.09.005","article-title":"Calcium Metabolism in Birds","volume":"11","year":"2008","journal-title":"Vet. Clin. N. Am. Exot. Anim. Pract."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/4\/662\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:34:59Z","timestamp":1760121299000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/4\/662"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,14]]},"references-count":30,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["ani13040662"],"URL":"https:\/\/doi.org\/10.3390\/ani13040662","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,14]]}}}