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Gut microbes and their metabolites can regulate the development of host innate and adaptive immune systems. Animal immune systems maintain intestinal symbiotic microbiota homeostasis. However, relatively few studies have been published on reptiles, particularly snakes, and even fewer studies on different parts of the digestive tracts of these animals. Herein, we used 16S rRNA gene sequencing to investigate the microbial community composition and adaptability in the stomach and small and large intestines of Lycodon rufozonatus. Proteobacteria, Bacteroidetes, and Firmicutes were most abundant in the stomach; Fusobacteria in the small intestine; and Proteobacteria, Bacteroidetes, Fusobacteria, and Firmicutes in the large intestine. No dominant genus could be identified in the stomach; however, dominant genera were evident in the small and large intestines. The microbial diversity index was significantly higher in the stomach than in the small and large intestines. Moreover, the influence of the microbial community structure on function was clarified through function prediction. Collectively, the gut microbes in the different segments of the digestive tract revealed the unique features of the L. rufozonatus gut microbiome. Our results provide insights into the co-evolutionary relationship between reptile gut microbiota and their hosts.<\/jats:p>","DOI":"10.3390\/ani13040731","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T05:57:52Z","timestamp":1676872672000},"page":"731","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Characteristics of Microbiota in Different Segments of the Digestive Tract of Lycodon rufozonatus"],"prefix":"10.3390","volume":"13","author":[{"given":"Yongquan","family":"Shang","sequence":"first","affiliation":[{"name":"College of Life Sciences, Qufu Normal University, Qufu 273165, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaming","family":"Zhong","sequence":"additional","affiliation":[{"name":"College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0730-7374","authenticated-orcid":false,"given":"Gang","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Qufu Normal University, Qufu 273165, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2036-5184","authenticated-orcid":false,"given":"Xibao","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Qufu Normal University, Qufu 273165, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyang","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Qufu Normal University, Qufu 273165, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinguo","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Qufu Normal University, Qufu 273165, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lupeng","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Qufu Normal University, Qufu 273165, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honghai","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Qufu Normal University, Qufu 273165, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"913700","DOI":"10.3389\/fmicb.2022.913700","article-title":"Multi-Omics Approaches Revealed the Associations of Host Metabolism and Gut Microbiome with Phylogeny and Environmental Adaptation in Mountain Dragons","volume":"13","author":"Zhu","year":"2022","journal-title":"Front. 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