{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:22:43Z","timestamp":1791462163513,"version":"4.3.4"},"reference-count":38,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,19]],"date-time":"2023-02-19T00:00:00Z","timestamp":1676764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Realm R&amp;D Program of GuangDong Province","award":["2020B0202080002"],"award-info":[{"award-number":["2020B0202080002"]}]},{"name":"Key Realm R&amp;D Program of GuangDong Province","award":["2020KCXTD019"],"award-info":[{"award-number":["2020KCXTD019"]}]},{"name":"Key Realm R&amp;D Program of GuangDong Province","award":["2021KJ115"],"award-info":[{"award-number":["2021KJ115"]}]},{"name":"Key Realm R&amp;D Program of GuangDong Province","award":["2022KJ115"],"award-info":[{"award-number":["2022KJ115"]}]},{"name":"Innovative Team Projects of Ordinary Colleges and Universities of Guangdong Province","award":["2020B0202080002"],"award-info":[{"award-number":["2020B0202080002"]}]},{"name":"Innovative Team Projects of Ordinary Colleges and Universities of Guangdong Province","award":["2020KCXTD019"],"award-info":[{"award-number":["2020KCXTD019"]}]},{"name":"Innovative Team Projects of Ordinary Colleges and Universities of Guangdong Province","award":["2021KJ115"],"award-info":[{"award-number":["2021KJ115"]}]},{"name":"Innovative Team Projects of Ordinary Colleges and Universities of Guangdong Province","award":["2022KJ115"],"award-info":[{"award-number":["2022KJ115"]}]},{"name":"Provincial-level agricultural technology innovation promotion and agricultural resources and ecological environmental protection construction projects","award":["2020B0202080002"],"award-info":[{"award-number":["2020B0202080002"]}]},{"name":"Provincial-level agricultural technology innovation promotion and agricultural resources and ecological environmental protection construction projects","award":["2020KCXTD019"],"award-info":[{"award-number":["2020KCXTD019"]}]},{"name":"Provincial-level agricultural technology innovation promotion and agricultural resources and ecological environmental protection construction projects","award":["2021KJ115"],"award-info":[{"award-number":["2021KJ115"]}]},{"name":"Provincial-level agricultural technology innovation promotion and agricultural resources and ecological environmental protection construction projects","award":["2022KJ115"],"award-info":[{"award-number":["2022KJ115"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The purpose of this study was to evaluate the potential effect of oxidative stress on the intestine of squabs, and to explore the molecular mechanisms. A total of 360 1-day-old squabs were divided evenly into five different groups (n = 72\/group): control, negative control, low, medium, and high dose groups. On the 3rd, 5th, and 7th days, squabs in the control group were not effectively treated and the negative control group were intraperitoneally injected with normal saline, whereas the H2O2 group was injected with H2O2 of 2.0, 2.5, and 3.0 mmol\/kg BW respectively. On the 21st day, the serum and duodenum were collected for further analysis. The results indicated that, compared with the control group, H2O2 caused squabs weight loss and intestinal morphology damage, and these effects were enhanced with an increase in dose. Further examination revealed that the contents of oxidative stress markers in both the serum and duodenum of the H2O2 group were significantly enhanced as the dose was increased. In addition, H2O2 exposure also resulted in the lower mRNA expression of Occludin, ZO-1, Beclin1, Atg5, and Caspase-3, but the expression of Claudin2 and Bcl-2 was decreased in comparison to the control group. These findings suggested that duodenal oxidative damage was accompanied by weight loss, changes in intestinal morphology, redox status imbalance, apoptosis as well as autophagy of intestinal cells, with, effects of 3.0 mmol\/kg BW of H2O2 being the most severe.<\/jats:p>","DOI":"10.3390\/ani13040749","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T05:57:52Z","timestamp":1676872672000},"page":"749","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Effects of Hydrogen Peroxide-Induced Oxidative Stress on Intestinal Morphology, Redox Status, and Related Molecules in Squabs"],"prefix":"10.3390","volume":"13","author":[{"given":"Yajing","family":"Zhong","sequence":"first","affiliation":[{"name":"Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"},{"name":"College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Ma","sequence":"additional","affiliation":[{"name":"Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"},{"name":"College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqi","family":"Fu","sequence":"additional","affiliation":[{"name":"Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"},{"name":"College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ailing","family":"Chen","sequence":"additional","affiliation":[{"name":"Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"},{"name":"College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahao","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhua","family":"Huang","sequence":"additional","affiliation":[{"name":"Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"},{"name":"College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Fu","sequence":"additional","affiliation":[{"name":"Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"},{"name":"College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Xu, Q., Li, H., Zhou, W., Zou, X., and Dong, X. 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