{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T02:43:14Z","timestamp":1790995394804,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,19]],"date-time":"2023-02-19T00:00:00Z","timestamp":1676764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Earmarked Fund for China Agriculture Research System","award":["CARS-42-11"],"award-info":[{"award-number":["CARS-42-11"]}]},{"name":"Earmarked Fund for China Agriculture Research System","award":["JATS [2022]496"],"award-info":[{"award-number":["JATS [2022]496"]}]},{"name":"the Jiangsu Agricultural Industry Technology System","award":["CARS-42-11"],"award-info":[{"award-number":["CARS-42-11"]}]},{"name":"the Jiangsu Agricultural Industry Technology System","award":["JATS [2022]496"],"award-info":[{"award-number":["JATS [2022]496"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study evaluated the slaughter performance, digestive tract development and tibia mineralization effects of sodium (Na) and chloride (Cl) on geese. Four hundred and thirty-four male geese at 29 days were randomly assigned into nine groups with six replicates (eight in each). The experiment employed a 3 \u00d7 3 factorial design, with two instances each of three Na levels (0.10%, 0.15%, and 0.20%) and three Cl levels (0.15%, 0.20%, and 0.25%). All experimental birds were husbanded for 42 days. Dietary Na and Cl levels and their interactions (Na \u00d7Cl) had no significant effect on the slaughter, breast, thigh, abdominal fat yield, and digestive tract index of geese (p &gt; 0.05). However, dietary Na and Cl level significantly affected the crypt depth of the jejunum and tibial development. Variations in Na and Cl levels had a significant interaction on the crypt depth of jejunal (p &lt; 0.05), 0.20% Na \u00d7 0.25% Cl had a minor crypt depth. Dietary variations in Na and Cl significantly affected the tibial strength, and there was a significant interaction between them (p &lt; 0.05). When Na and Cl were at their maximum (0.20% Na and 0.25% Cl), the strength of the tibia was the lowest. In addition, a single factor (Na or Cl) had no effect (p &gt; 0.05), but its interaction significantly affected the calcium (Ca) content of bone (p &lt; 0.05). When the Na and Cl levels were 0.15% and 0.15%, respectively, the Ca content in bone was the highest. These results suggest that dietary Na and Cl had interactive effects on geese, especially in the development of the tibia. High dietary Na and Cl levels adversely influenced the tibia and intestinal crypt morphology. Therefore, we do not advocate supplementing too much Na or Cl in the diet. Combined with our previous results, for 29\u201370-day-old geese, it is recommended that dietary Na and Cl levels should be 0.10% and 0.15%, respectively.<\/jats:p>","DOI":"10.3390\/ani13040751","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T05:57:52Z","timestamp":1676872672000},"page":"751","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effects of Dietary Sodium and Chloride on Slaughter Performance, Digestive Tract Development and Tibia Mineralization of Geese"],"prefix":"10.3390","volume":"13","author":[{"given":"Yuanjing","family":"Chen","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"},{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5608-538X","authenticated-orcid":false,"given":"Zhiyue","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiming","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"432","DOI":"10.3382\/japr.2008-00061","article-title":"Impact of lameness on broiler well-being","volume":"18","author":"Paz","year":"2009","journal-title":"J. Appl. Poult. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1790","DOI":"10.3382\/ps.2011-01968","article-title":"The effects of growth rate on leg morphology and tibia breaking strength, mineral density, mineral content, and bone ash in broilers","volume":"91","author":"Shim","year":"2012","journal-title":"Poult. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1080\/00071667808416503","article-title":"Interrelationships between dietary concentrations of sodium, potassium and chloride in laying hens","volume":"19","author":"Sauveur","year":"1978","journal-title":"Brit. Poult. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1079\/PNS19810045","article-title":"Recent advances in dietary anion-cation balance: Applications in poultry","volume":"40","author":"Mongin","year":"1981","journal-title":"Proc. Nutr. Soc."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"299","DOI":"10.3382\/ps.0660299","article-title":"Effects of dietary crude protein, electrolyte balance, and photoperiod on growth of broiler chickens","volume":"66","author":"Adekunmisi","year":"1987","journal-title":"Poult. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1093\/ps\/86.7.1363","article-title":"Electrolyte diets, stress, and acid-base balance in broiler chickens","volume":"86","author":"Olanrewaju","year":"2007","journal-title":"Poult. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1093\/jn\/114.6.1001","article-title":"Studies on the etiology of tibial Dyschondroplasia in chickens","volume":"114","author":"Edwards","year":"1984","journal-title":"J. Nutr."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1172\/JCI112344","article-title":"Metabolic acidosis stimulates protein degradation in rat muscle by a glucocorticoid-dependent mechanism","volume":"77","author":"May","year":"1986","journal-title":"J. Clin. Investig."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1111\/jpn.13541","article-title":"The effect of different dietary levels of sodium and chloride on performance, blood parameters and excreta quality in goslings at 29 to 70 days of age","volume":"106","author":"Chen","year":"2021","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"382","DOI":"10.3382\/ps.2012-02404","article-title":"Effect of different magnesium sources on digesta and excreta moisture content and production performance in broiler chickens","volume":"92","author":"Linde","year":"2013","journal-title":"Poult. Sci."},{"key":"ref_11","first-page":"108","article-title":"Effect of stocking density on performance, infection by Eimeria spp., intestinal lesions and foot pad injuries in broilers with outdoor access under tropical conditions","volume":"63","year":"2021","journal-title":"Brit. Poult. Sci."},{"key":"ref_12","first-page":"208","article-title":"Growth responses, excreta quality, nutrient digestibility, bone development and meat yield traits of broiler chickens fed vegetable or animal protein diets","volume":"43","author":"Hossain","year":"2013","journal-title":"S. Afr. J. Anim. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.1080\/1828051X.2020.1846468","article-title":"Evaluation of interactive effects of phytase and benzoic acid supplementation on performance, nutrients digestibility, tibia mineralisation, gut morphology and serum traits in male broiler chickens","volume":"19","author":"Karami","year":"2020","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1093\/ps\/80.3.289","article-title":"Sodium and chloride requirements of growing broiler chickens (twenty-one to forty-two days of age) fed corn-soybean diets","volume":"80","author":"Murakami","year":"2001","journal-title":"Poult. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"93","DOI":"10.22358\/jafs\/66161\/2011","article-title":"The effect of different dietary sodium levels on the growth performance of broiler chickens, gastrointestinal function, excreta moisture and tibia mineralization","volume":"20","author":"Jankowski","year":"2011","journal-title":"J. Anim. Feed Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1113\/expphysiol.1991.sp003540","article-title":"Avian lower intestine adapts to dietary salt (NaCl) depletion by increasing transepithelial sodium transport and microvillous membrane surface area","volume":"76","author":"Elbrond","year":"1991","journal-title":"Exp. Physiol."},{"key":"ref_17","unstructured":"NRC (1994). Nutrient Requirements of Poultry, National Research Council, National Academy of Science. [9th ed.]."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2066","DOI":"10.3382\/ps\/pey553","article-title":"Effects of cottonseed meal on growth performance, small intestinal morphology, digestive enzyme activities, and serum biochemical parameters of geese","volume":"98","author":"Yu","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_19","unstructured":"AOAC (2000). Official Methods of Analysis of AOAC International, AOAC. [17th ed.]."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/00103627009366233","article-title":"Potentiometric titration of chloride in plant tissue extracts using the chloride ion electrode","volume":"1","author":"Lacroix","year":"1970","journal-title":"Commun. Soil Sci. Plant Anal."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1716","DOI":"10.1093\/ps\/84.11.1716","article-title":"Effect and interactions of dietary sodium and chloride on broiler starter performance (hatching to twenty-eight days of age) under subtropical summer conditions","volume":"84","author":"Mushtaq","year":"2005","journal-title":"Poult. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.3382\/ps.0641060","article-title":"Chronic heat stress and respiratory alkalosis: Occurrence and treatment in broiler chicks","volume":"64","author":"Teeter","year":"1985","journal-title":"Poult. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"845","DOI":"10.5713\/ajas.2012.12664","article-title":"Growth performance, carcass characteristics and plasma mineral chemistry as affected by dietary chloride and chloride salts fed to broiler chickens reared under phase feeding system","volume":"26","author":"Mushtaq","year":"2013","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2161","DOI":"10.17582\/journal.pjz\/2017.49.6.2161.2171","article-title":"Effect of adding different levels of dietary electrolyte in broiler rations using sodium bicarbonate as a source of electrolyte","volume":"49","author":"Yasoob","year":"2017","journal-title":"Pak. J. Zool."},{"key":"ref_25","first-page":"959","article-title":"Effect of feeding periods and sodium levels of pre-starter diet on broiler performance and serum electrolytes","volume":"6","author":"Bidar","year":"2007","journal-title":"J. Anim. Vet. Adv."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1093\/japr\/16.2.161","article-title":"Dietary sodium and chloride for twenty-nine-to forty-two-day-old broiler chickens at constant electrolyte balance under subtropical summer conditions","volume":"16","author":"Mushtaq","year":"2007","journal-title":"J. Appl. Poult. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/s10633-008-9128-7","article-title":"The effect of oxygen and light on the structure and function of the neonatal rat retina","volume":"118","author":"Dorfman","year":"2009","journal-title":"Doc. Ophthalmol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"851","DOI":"10.3923\/ijps.2010.851.855","article-title":"Post hatch histo-morphological studies of small intestinal development in chicks fed with herbal early chick nutritional supplement","volume":"9","author":"Yadav","year":"2010","journal-title":"Int. J. Poult. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1700443","DOI":"10.1002\/pmic.201700443","article-title":"Impacts of Dietary Pleurotus eryngii Polysaccharide on Nutrient Digestion, Metabolism, and Immune Response of the Small Intestine and Colon\u2014An iTRAQ-Based Proteomic Analysis","volume":"18","author":"Ma","year":"2018","journal-title":"Proteomics"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"197","DOI":"10.2141\/jpsa.0140152","article-title":"Protein source and nutrient density in the diets of male broilers from 8 to 21 days of age: Effects on tibia dimension, breaking strength, and mineral density","volume":"52","author":"Wang","year":"2015","journal-title":"J. Poult. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1093\/ps\/84.2.283","article-title":"Impact of glutamine and oasis hatchling supplement on growth performance, small intestinal morphology, and immune response of broilers vaccinated and challenged with Eimeria maxima","volume":"84","author":"Yi","year":"2005","journal-title":"Poult. Sci."},{"key":"ref_32","first-page":"299","article-title":"Physiology and pathophysiology of intestinal absorption","volume":"55","author":"Caspary","year":"1992","journal-title":"AJCN"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2614","DOI":"10.3382\/ps.2012-02244","article-title":"Bone characteristics of late-term embryonic and hatchling broilers: Bone development under extreme growth rate","volume":"91","author":"Yair","year":"2012","journal-title":"Poult. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1093\/ps\/78.3.366","article-title":"Microbial phytase in finisher diets of White Pekin ducks: Effects on growth performance, plasma phosphorus concentration, and leg bone characteristics","volume":"78","author":"Orban","year":"1999","journal-title":"Poult. Sci."},{"key":"ref_35","first-page":"33","article-title":"Study on the growth and development of tibia and the regularity of calcium and phosphorus deposition in Jilin white goose","volume":"33","author":"Ji","year":"2011","journal-title":"China Poult."},{"key":"ref_36","first-page":"79","article-title":"Research progress on factors affecting bone metabolism in poultry","volume":"17","author":"Yan","year":"2017","journal-title":"Heilongjiang Anim. Husb. Vet. Med."},{"key":"ref_37","first-page":"519","article-title":"Influence of sodium, chloride and calcium level and their interaction on performance and deformity of tibia bone of broiler chicks","volume":"28","author":"Sayed","year":"2008","journal-title":"Egypt. Poult. Sci. J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1093\/jn\/102.12.1673","article-title":"Further studies on tibial dyschondroplasia (cartilage abnormality) in young chicks","volume":"102","author":"Leach","year":"1972","journal-title":"J. Nutr."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.beem.2013.04.009","article-title":"Control of renal calcium, phosphate, Electrolyte, and water excretion by the calcium-sensing receptor","volume":"27","author":"Miller","year":"2013","journal-title":"Best Pract. Res. Clin. Endocrinol. Metab."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/4\/751\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:36:34Z","timestamp":1760121394000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/4\/751"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,19]]},"references-count":39,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["ani13040751"],"URL":"https:\/\/doi.org\/10.3390\/ani13040751","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,19]]}}}