{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T13:57:37Z","timestamp":1791899857206,"version":"4.3.4"},"reference-count":75,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T00:00:00Z","timestamp":1676851200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The objective of this experiment was to evaluate the impact of maize co-ensiling with increasing percentages of MOL forage on the kinetics of biogas, methane (CH4), carbon monoxide (CO) and hydrogen sulfide (H2S) production, as well as the characteristics of ruminal fermentation and CH4 conversion efficiency, using steers (STI) and sheep (SHI) as inoculum sources. With the STI, the inclusion of MOL reduced (linear: p \u2264 0.0199; quadratic: p \u2264 0.0267) biogas production (mL g\u22121 DM incubated and degraded), CH4 (mL g\u22121 DM degraded), CO (mL g\u22121 DM degraded), and H2S (mL g\u22121 DM incubated and degraded), without affecting (p &gt; 0.05) the parameters (b = asymptotic gas, c = rate of gas production and Lag = initial delay time before gas production) of CH4 and H2S, and the proportion and production of CH4 per kg of dry matter (DM). In addition, with this inoculum, pH, and dry matter degradation (DMD) increased (linear: p \u2264 0.0060), and although short-chain fatty acids (SCFA) and metabolizable energy (ME) decreased (linear: p &lt; 0.0001; quadratic: p \u2264 0.0015), this did not affect (p &gt; 0.05) the CH4 conversion efficiency. Meanwhile, with the SHI, the inclusion of MOL only decreased (linear: p \u2264 0.0206; quadratic: p \u2264 0.0003) biogas per dry matter (DM) degraded and increased (linear: p \u2264 0.0293; quadratic: p \u2264 0.0325) biogas per DM incubated, as well as the production (mL g\u22121 DM incubated and degraded and g\u22121 kg DM) and proportion of CH4, and CO per DM incubated and degraded. In addition, it did not impact (p &gt; 0.05) on the CH4 and H2S parameters, and in the H2S by DM incubated and degraded, and although it increased (linear: p \u2264 0.0292; quadratic: p \u2264 0.0325) the DMD, SCFA, and ME, it was inefficient (quadratic: p \u2264 0.0041) in CH4 conversion. It is concluded that regardless of the percentage of MOL, the STI presented the highest values in the production of biogas, CH4, H2S, DMD, SCFA, and ME, and the lowest pH, so it turned out to be the most efficient in CH4 conversion, while with the SHI only the highest production of CO and pH was obtained, and the lowest DMD, SCFA, and ME, so it was less efficient compared to STI.<\/jats:p>","DOI":"10.3390\/ani13040764","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T06:37:59Z","timestamp":1676875079000},"page":"764","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Impact of Co-Ensiling of Maize with Moringa oleifera on the Production of Greenhouse Gases and the Characteristics of Fermentation in Ruminants"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1265-6627","authenticated-orcid":false,"given":"Edwin Rafael","family":"Alvarado-Ram\u00edrez","sequence":"first","affiliation":[{"name":"Faculty of Agronomy and Veterinary, Autonomous University of San Luis Potos\u00ed, 78321 San Luis Potos\u00ed, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7128-8556","authenticated-orcid":false,"given":"Aristide","family":"Maggiolino","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Bari A. Moro, 70010 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona M. M. Y.","family":"Elghandour","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine and Zootechnics, Autonomous University of the State of Mexico, 50000 Toluca, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3356-1499","authenticated-orcid":false,"given":"Marco Antonio","family":"Rivas-Jacobo","sequence":"additional","affiliation":[{"name":"Faculty of Agronomy and Veterinary, Autonomous University of San Luis Potos\u00ed, 78321 San Luis Potos\u00ed, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilberto","family":"Ballesteros-Rodea","sequence":"additional","affiliation":[{"name":"Faculty of Agronomy and Veterinary, Autonomous University of San Luis Potos\u00ed, 78321 San Luis Potos\u00ed, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5612-1691","authenticated-orcid":false,"given":"Pasquale De","family":"Palo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Bari A. Moro, 70010 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7418-4170","authenticated-orcid":false,"given":"Abdelfattah Z. M.","family":"Salem","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine and Zootechnics, Autonomous University of the State of Mexico, 50000 Toluca, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"18237","DOI":"10.1073\/pnas.1012541107","article-title":"Livestock production and the global environment: Consume less or produce better?","volume":"107","author":"Steinfeld","year":"2010","journal-title":"Proc. Natl. Acad. Sci. 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