{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T13:57:31Z","timestamp":1792245451245,"version":"4.4.0"},"reference-count":164,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,4,4]],"date-time":"2023-04-04T00:00:00Z","timestamp":1680566400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund (FWF) project","doi-asserted-by":"publisher","award":["P 32340-B"],"award-info":[{"award-number":["P 32340-B"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund (FWF) project","doi-asserted-by":"publisher","award":["PP291"],"award-info":[{"award-number":["PP291"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Veterinary Medicine, Vienna, Austria","award":["P 32340-B"],"award-info":[{"award-number":["P 32340-B"]}]},{"name":"University of Veterinary Medicine, Vienna, Austria","award":["PP291"],"award-info":[{"award-number":["PP291"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Gene editing and gene silencing techniques have the potential to revolutionize our knowledge of biology and diseases of fish and other aquatic animals. By using such techniques, it is feasible to change the phenotype and modify cells, tissues and organs of animals in order to cure abnormalities and dysfunctions in the organisms. Gene editing is currently experimental in wide fields of aquaculture, including growth, controlled reproduction, sterility and disease resistance. Zink finger nucleases, TALENs and CRISPR\/Cas9 targeted cleavage of the DNA induce favorable changes to site-specific locations. Moreover, gene silencing can be used to inhibit the translation of RNA, namely, to regulate gene expression. This methodology is widely used by researchers to investigate genes involved in different disorders. It is a promising tool in biotechnology and in medicine for investigating gene function and diseases. The production of food fish has increased markedly, making fish and seafood globally more popular. Consequently, the incidence of associated problems and disease outbreaks has also increased. A greater investment in new technologies is therefore needed to overcome such problems in this industry. To put it concisely, the modification of genomic DNA and gene silencing can comprehensively influence aquatic animal medicine in the future. On the ethical side, these precise genetic modifications make it more complicated to recognize genetically modified organisms in nature and can cause several side effects through created mutations. The aim of this review is to summarize the current state of applications of gene modifications and genome editing in fish medicine.<\/jats:p>","DOI":"10.3390\/ani13071250","type":"journal-article","created":{"date-parts":[[2023,4,5]],"date-time":"2023-04-05T01:39:26Z","timestamp":1680658766000},"page":"1250","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Review: Recent Applications of Gene Editing in Fish Species and Aquatic Medicine"],"prefix":"10.3390","volume":"13","author":[{"given":"Anik\u00f3","family":"Gut\u00e1si","sequence":"first","affiliation":[{"name":"Department of Farm Animals and Veterinary Public Health, Division of Fish Health, University of Veterinary Medicine, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1475-3938","authenticated-orcid":false,"given":"Sabine E.","family":"Hammer","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, Institute of Immunology, University of Veterinary Medicine, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8148-0218","authenticated-orcid":false,"given":"Mansour","family":"El-Matbouli","sequence":"additional","affiliation":[{"name":"Department of Farm Animals and Veterinary Public Health, Division of Fish Health, University of Veterinary Medicine, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9273-1502","authenticated-orcid":false,"given":"Mona","family":"Saleh","sequence":"additional","affiliation":[{"name":"Department of Farm Animals and Veterinary Public Health, Division of Fish Health, University of Veterinary Medicine, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"103547","DOI":"10.1016\/j.marpol.2019.103547","article-title":"Misunderstandings, myths and mantras in aquaculture: Its contribution to world food supplies has been systematically over reported","volume":"106","author":"Edwards","year":"2019","journal-title":"Mar. Policy"},{"key":"ref_2","unstructured":"FAO (2022). The State of World Fisheries and Aquaculture 2022, FAO. Towards Blue Transformation."},{"key":"ref_3","unstructured":"Food and Agriculture Organization (FAO) (2014). FAO: Fisheries and Aquaculture Statistics, FAO."},{"key":"ref_4","unstructured":"FAO (2012). The State of World Fisheries and Aquaculture 2012, FAO. FAO Fisheries Technical Paper."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1111\/jwas.12714","article-title":"Achieving sustainable aquaculture: Historical and current perspectives and future needs and challenges","volume":"51","author":"Boyd","year":"2020","journal-title":"J. World Aquac. Soc."},{"key":"ref_6","unstructured":"World Bank (2013). Fish to 2030: Prospects for Fisheries and Aquaculture\u2014World Bank Report Number 83177-GLB, World Bank. Agriculture and Environmental Services Discussion Paper."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.jgg.2016.04.012","article-title":"The Applications of Genome Editing in Xenotransplantation","volume":"43","author":"Perota","year":"2016","journal-title":"J. Genet. Genom."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1038\/mt.2008.114","article-title":"Zinc-finger Nucleases: The Next Generation Emerges","volume":"16","author":"Cathomen","year":"2008","journal-title":"Mol. Ther."},{"key":"ref_9","unstructured":"Malik, Y.S., Barh, D., Azevedo, V.A.D.C., and Khurana, S.P. (2020). Genomics and Biotechnological Advances in Veterinary, Poultry, and Fisheries, Elsevier Inc."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1016\/j.tig.2019.06.006","article-title":"Potential of Genome Editing to Improve Aquaculture Breeding and Production","volume":"35","author":"Gratacap","year":"2019","journal-title":"Trends Genet."},{"key":"ref_11","unstructured":"Karre, A. (2023, March 22). Gene Editing Technology. Available online: https:\/\/www.researchgate.net\/publication\/347442835_GENE_EDITING_TECHNOLOGY."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1126\/science.1079512","article-title":"Enhancing Gene Targeting with Designed Zinc Finger Nucleases","volume":"300","author":"Bibikova","year":"2003","journal-title":"Science"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1126\/science.1178817","article-title":"A Simple Cipher Governs DNA Recognition by TAL Effectors","volume":"326","author":"Moscou","year":"2009","journal-title":"Science"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1038\/nbt.3692","article-title":"The emerging patent landscape of CRISPR\u2013Cas gene editing technology","volume":"34","author":"Egelie","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Xu, X., Hulshoff, M.S., Tan, X., Zeisberg, M., and Zeisberg, E.M. (2020). CRISPR\/Cas Derivatives as Novel Gene Modulating Tools: Possibilities and In Vivo Applications. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21093038"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"5497","DOI":"10.1093\/emboj\/17.18.5497","article-title":"Homologous recombination and non-homologous end-joining pathways of DNA double-strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells","volume":"17","author":"Takata","year":"1998","journal-title":"EMBO J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"R455","DOI":"10.1016\/S0960-9822(01)00279-2","article-title":"Non-homologous end joining as a mechanism of DNA repair","volume":"11","author":"Barnes","year":"2001","journal-title":"Curr. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1146\/annurev.biochem.052308.093131","article-title":"The Mechanism of Double-Strand DNA Break Repair by the Nonhomologous DNA End-Joining Pathway","volume":"79","author":"Lieber","year":"2010","journal-title":"Annu. Rev. Biochem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1756-8935-5-4","article-title":"ATP-dependent chromatin remodeling in the DNA-damage response","volume":"5","author":"Lans","year":"2012","journal-title":"Epigenetics Chromatin"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/nbt.3055","article-title":"In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9","volume":"33","author":"Swiech","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"ref_21","first-page":"130","article-title":"DNA double strand break repair via non-homologous end-joining","volume":"2","author":"Davis","year":"2013","journal-title":"Transl. Cancer Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1038\/nbt.3198","article-title":"Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells","volume":"33","author":"Chu","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.gpb.2016.05.002","article-title":"DNA End Resection: Facts and Mechanisms","volume":"14","author":"Liu","year":"2016","journal-title":"Genom. Proteom. Bioinform."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3683","DOI":"10.1093\/nar\/gkh703","article-title":"Conservative homologous recombination preferentially repairs DNA double-strand breaks in the S phase of the cell cycle in human cells","volume":"32","author":"Helleday","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"ref_25","unstructured":"Bharati, J., Punetha, M., Kumar, B.S., Vidyalakshmi, G., Sarkar, M., D\u2019Occhio, M.J., and Singh, R.K. (2019). Genomics and Biotechnological Advances in Veterinary, Poultry, and Fisheries, Elsevier Inc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1038\/372642a0","article-title":"In vivo repression by a site-specific DNA-binding protein designed against an oncogenic sequence","volume":"372","author":"Choo","year":"1994","journal-title":"Nature"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1073\/pnas.93.3.1156","article-title":"Hybrid restriction enzymes: Zinc finger fusions to Fok I cleavage domain","volume":"93","author":"Kim","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1038\/nrg2842","article-title":"Genome editing with engineered zinc finger nucleases","volume":"11","author":"Urnov","year":"2010","journal-title":"Nat. Rev. Genet."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1166\/gge.2015.1010","article-title":"Advances in Zinc Finger Nuclease and Its Applications","volume":"1","author":"Tang","year":"2015","journal-title":"Gene Gene Ed."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1146\/annurev-biochem-060713-035418","article-title":"Genome Engineering with Targetable Nucleases","volume":"83","author":"Carroll","year":"2014","journal-title":"Annu. Rev. Biochem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1128\/MMBR.00015-06","article-title":"A Fungal Family of Transcriptional Regulators: The Zinc Cluster Proteins","volume":"70","author":"MacPherson","year":"2006","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"5525","DOI":"10.1073\/pnas.94.11.5525","article-title":"Design of polydactyl zinc-finger proteins for unique addressing within complex genomes","volume":"94","author":"Liu","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1038\/gt.2012.2","article-title":"Zinc finger nucleases: Looking toward translation","volume":"20","author":"Palpant","year":"2013","journal-title":"Gene Ther."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1038\/nbt1398","article-title":"Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases","volume":"26","author":"Meng","year":"2008","journal-title":"Nat. Biotechnol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1038\/nature03556","article-title":"Highly efficient endogenous human gene correction using designed zinc-finger nucleases","volume":"435","author":"Urnov","year":"2005","journal-title":"Nature"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1038\/nbt.1562","article-title":"Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases","volume":"27","author":"Hockemeyer","year":"2009","journal-title":"Nat. Biotechnol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1038\/nrm3486","article-title":"TALENs: A widely applicable technology for targeted genome editing","volume":"14","author":"Joung","year":"2013","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1126\/science.1178811","article-title":"Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors","volume":"326","author":"Boch","year":"2009","journal-title":"Science"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1146\/annurev-phyto-080508-081936","article-title":"Xanthomonas AvrBs3 Family-Type III Effectors: Discovery and Function","volume":"48","author":"Boch","year":"2010","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/nbt.1755","article-title":"A TALE nuclease architecture for efficient genome editing","volume":"29","author":"Miller","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"9779","DOI":"10.1093\/nar\/gkt754","article-title":"Directed evolution of the TALE N-terminal domain for recognition of all 5\u2032 bases","volume":"41","author":"Lamb","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2623","DOI":"10.1073\/pnas.1019533108","article-title":"De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaks","volume":"108","author":"Mahfouz","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1126\/science.1225829","article-title":"A Programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity","volume":"337","author":"Jinek","year":"2012","journal-title":"Science"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3497","DOI":"10.1038\/s41467-018-05843-9","article-title":"Cas9 immunity creates challenges for CRISPR gene editing therapies","volume":"9","author":"Crudele","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Lucas, J.S., Southgate, P.C., and Tucker, C.S. (2013). Aquaculture: Farming Aquatic Animals and Plants, Wiley-Blackwell. [2nd ed.].","DOI":"10.1002\/9781118687932"},{"key":"ref_46","unstructured":"Pankaj, C.Y. (2014). Cell Stem Cell. Sci Rep., 331\u2013335."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1038\/nmeth.2649","article-title":"Cas9 as a versatile tool for engineering biology","volume":"10","author":"Mali","year":"2013","journal-title":"Nat. Methods"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"W52","DOI":"10.1093\/nar\/gkm360","article-title":"CRISPRFinder: A web tool to identify clustered regularly interspaced short palindromic repeats","volume":"35","author":"Grissa","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1126\/science.1159689","article-title":"Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes","volume":"321","author":"Brouns","year":"2008","journal-title":"Science"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1038\/nrmicro2577","article-title":"Evolution and classification of the CRISPR\u2013Cas systems","volume":"9","author":"Makarova","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1038\/nature10402","article-title":"Structures of the RNA-guided surveillance complex from a bacterial immune system","volume":"477","author":"Wiedenheft","year":"2011","journal-title":"Nature"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.scib.2017.01.003","article-title":"Gene editing nuclease and its application in tilapia","volume":"62","author":"Li","year":"2017","journal-title":"Sci. Bull."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1247997","DOI":"10.1126\/science.1247997","article-title":"Structures of Cas9 endonukleases reveal RNA-mediated conformational activation","volume":"343","author":"Jinek","year":"2014","journal-title":"Science"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1038\/nmeth.3113","article-title":"Genome engineering: The next genomic revolution","volume":"11","author":"Gersbach","year":"2014","journal-title":"Nat. Methods"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1016\/j.biochi.2003.10.011","article-title":"Repair of DNA double strand breaks by non-homologous end joining","volume":"85","author":"Meek","year":"2003","journal-title":"Biochimie"},{"key":"ref_56","unstructured":"Singh, I.P., Hasan, S., and Saxena, P. (2019). RNAi: Gene Silencing. Int. J. Res. Eng. Sci. Manag., 52\u201354."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"A224","DOI":"10.1289\/ehp.112-a224","article-title":"RNAi: What\u2019s all the noise about gene silencing?","volume":"112","author":"Hood","year":"2004","journal-title":"Environ. Health Perspect."},{"key":"ref_58","unstructured":"Daneholt, B. (2006). Advanced Information on the Nobel Prize in Physiology or Medicine 2006, The Nobel Assembly at Karolinska Institute Karolinska Institute."},{"key":"ref_59","unstructured":"Estrada, M.P., Lugo, J.M., and Carpio, Y. (2008). RNA Interference: Methods for Plants and Animals, CABI."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1096\/fj.06-6014rev","article-title":"A brief history of RNAi: The silence of the genes","volume":"20","author":"Sen","year":"2006","journal-title":"FASEB J."},{"key":"ref_61","unstructured":"Singh, D., Chaudhary, S., Kumar, R., Sirohi, P., Mehla, K., Sirohi, A., Kumar, S., Chand, P., and Singh, P.K. (2016). RNA Interference, Opentech."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1038\/nrm4085","article-title":"The expanding world of small RNAs in plants","volume":"16","author":"Borges","year":"2015","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"200","DOI":"10.3389\/fpls.2017.00200","article-title":"RNA Interference (RNAi) as a Potential Tool for Control of Mycotoxin Contamination in Crop Plants: Concepts and Considerations","volume":"8","author":"Majumdar","year":"2017","journal-title":"Front. Plant Sci."},{"key":"ref_64","first-page":"1","article-title":"Gene Silencing and DNA Methylation","volume":"2","author":"Parveen","year":"2019","journal-title":"Am. J. Phytomedicine Clin. Therapeutics."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"5427","DOI":"10.1021\/nn2005574","article-title":"DNA Cage Delivery to Mammalian Cells","volume":"5","author":"Walsh","year":"2011","journal-title":"ACS Nano"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"3529","DOI":"10.1002\/smll.201101804","article-title":"Design, Assembly, and Activity of Antisense DNA Nanostructures","volume":"7","author":"Keum","year":"2011","journal-title":"Small"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.bbrc.2005.06.063","article-title":"Silencing of yellow head virus replication in penaeid shrimp cells by dsRNA","volume":"334","author":"Tirasophon","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"4385","DOI":"10.1111\/j.1742-4658.2007.05968.x","article-title":"The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis","volume":"274","author":"Tiu","year":"2007","journal-title":"FEBS J."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"W124","DOI":"10.1093\/nar\/gkh442","article-title":"siDirect: Highly effective, target-specific siRNA design software for mammalian RNA interference","volume":"32","author":"Naito","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1038\/418244a","article-title":"RNA interference","volume":"418","author":"Hannon","year":"2002","journal-title":"Nature"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1007\/s11373-007-9189-8","article-title":"Knock down of gfp and no tail expression in zebrafish embryo by in vivo-transcribed short hairpin RNA with T7 plasmid system","volume":"14","author":"Wang","year":"2007","journal-title":"J. Biomed. Sci."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Gotesman, M., Menanteau-Ledouble, S., Saleh, M., Bergmann, S.M., and El-Matbouli, M. (2018). A new age in AquaMedicine: Unconventional approach in studying aquatic diseases. BMC Vet. Res., 14.","DOI":"10.1186\/s12917-018-1501-5"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11248-021-00274-7","article-title":"Sustainable use of CRISPR\/Cas in fish aquaculture: The biosafety perspective","volume":"31","author":"Okoli","year":"2021","journal-title":"Transgenic Res."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1038\/nrg2091","article-title":"Animal models of human disease: Zebrafish swim into view","volume":"8","author":"Lieschke","year":"2007","journal-title":"Nat. Rev. Genet."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/s13578-015-0006-1","article-title":"Targeted gene disruption in Xenopus laevis using CRISPR\/Cas9","volume":"5","author":"Wang","year":"2015","journal-title":"Cell Biosci."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1038\/nbt1409","article-title":"Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases","volume":"26","author":"Doyon","year":"2008","journal-title":"Nat. Biotechnol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1101\/gr.161638.113","article-title":"Highly efficient CRISPR\/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair","volume":"24","author":"Auer","year":"2014","journal-title":"Genome Res."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Edvardsen, R., Leininger, S., Kleppe, L., Skaftnesmo, K.O., and Wargelius, A. (2014). Targeted Mutagenesis in Atlantic Salmon (Salmo salar L.) Using the CRISPR\/Cas9 System Induces Complete Knockout Individuals in the F0 Generation. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0108622"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1007\/s10126-014-9584-x","article-title":"Efficient Knockout of Transplanted Green Fluorescent Protein Gene in Medaka Using TALENs","volume":"16","author":"Qiu","year":"2014","journal-title":"Mar. Biotechnol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1534\/genetics.114.163667","article-title":"Efficient and Heritable Gene Targeting in Tilapia by CRISPR\/Cas9","volume":"197","author":"Li","year":"2014","journal-title":"Genetics"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1038\/nature751","article-title":"DMY is a Y-specific DM-domain gene required for male development in the medaka fish","volume":"417","author":"Matsuda","year":"2002","journal-title":"Nature"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1210\/en.2013-1642","article-title":"Whole Brain-Pituitary In Vitro Preparation of the Transgenic Medaka (Oryzias latipes) as a Tool for Analyzing the Differential Regulatory Mechanisms of LH and FSH Release","volume":"155","author":"Karigo","year":"2014","journal-title":"Endocrinology"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"3394","DOI":"10.1210\/en.2011-2022","article-title":"Time-of-Day-Dependent Changes in GnRH1 Neuronal Activities and Gonadotropin mRNA Expression in a Daily Spawning Fish, Medaka","volume":"153","author":"Karigo","year":"2012","journal-title":"Endocrinology"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1038\/269338a0","article-title":"Gonadotrophin-releasing hormone deficiency in a mutant mouse with hypogonadism","volume":"269","author":"Cattanach","year":"1977","journal-title":"Nature"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"3994","DOI":"10.1210\/en.2016-1356","article-title":"Evolution of the Hypothalamic-Pituitary-Gonadal Axis Regulation in Vertebrates Revealed by Knockout Medaka","volume":"157","author":"Takahashi","year":"2016","journal-title":"Endocrinology"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1007\/s10126-016-9687-7","article-title":"Editing of the Luteinizing Hormone Gene to Sterilize Channel Catfish, Ictalurus punctatus, Using a Modified Zinc Finger Nuclease Technology with Electroporation","volume":"18","author":"Qin","year":"2016","journal-title":"Mar. Biotechnol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1093\/icesjms\/fsu132","article-title":"Risk assessment of the environmental impact of Norwegian Atlantic salmon farming","volume":"72","author":"Taranger","year":"2015","journal-title":"ICES J. Mar. Sci."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Glover, K.A., Quintela, M., Wennevik, V., Besnier, F., S\u00f8rvik, A.G.E., and Skaala, \u00d8. (2012). Three Decades of Farmed Escapees in the Wild: A Spatio-Temporal Analysis of Atlantic Salmon Population Genetic Structure throughout Norway. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0043129"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"21284","DOI":"10.1038\/srep21284","article-title":"Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon","volume":"6","author":"Wargelius","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1038\/nature03595","article-title":"The Ter mutation in the dead end gene causes germ cell loss and testicular germ cell tumours","volume":"435","author":"Youngren","year":"2005","journal-title":"Nature"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"1154","DOI":"10.1210\/en.2005-1282","article-title":"Minireview: Kisspeptin Neurons as Central Processors in the Regulation of Gonadotropin-Releasing Hormone Secretion","volume":"147","author":"Dungan","year":"2006","journal-title":"Endocrinology"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S0014-5793(99)00009-5","article-title":"Discovery of a receptor related to the galanin receptors","volume":"446","author":"Lee","year":"1999","journal-title":"FEBS Lett."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1146\/annurev.physiol.70.113006.100540","article-title":"The Role of Kisspeptins and GPR54 in the Neuroendocrine Regulation of Reproduction","volume":"70","author":"Popa","year":"2008","journal-title":"Annu. Rev. Physiol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1210\/er.2009-0005","article-title":"Kisspeptin Signaling in the Brain","volume":"30","author":"Oakley","year":"2009","journal-title":"Endocr. Rev."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.yfrne.2007.07.002","article-title":"New frontiers in kisspeptin\/GPR54 physiology as fundamental gatekeepers of reproductive function","volume":"29","author":"Roa","year":"2008","journal-title":"Front. Neuroendocr."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1210\/en.2014-1204","article-title":"The kiss\/kissr Systems Are Dispensable for Zebrafish Reproduction: Evidence From Gene Knockout Studies","volume":"156","author":"Tang","year":"2015","journal-title":"Endocrinology"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"22953","DOI":"10.1038\/srep22953","article-title":"Targeted disruption of sp7 and myostatin with CRISPR-Cas9 results in severe bone defects and more muscular cells in common carp","volume":"6","author":"Zhong","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Dahlem, T.J., Hoshijima, K., Jurynec, M.J., Gunther, D., Starker, C., Locke, A.S., Weis, A., Voytas, D., and Grunwald, D.J. (2012). Simple Methods for Generating and Detecting Locus-Specific Mutations Induced with TALENs in the Zebrafish Genome. PLOS Genet., 8.","DOI":"10.1371\/journal.pgen.1002861"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"13904","DOI":"10.1073\/pnas.1308335110","article-title":"Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system","volume":"110","author":"Jao","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_100","doi-asserted-by":"crossref","unstructured":"Ma, L., Jeffery, W.R., Essner, J.J., and Kowalko, J.E. (2015). Genome Editing Using TALENs in Blind Mexican Cavefish, Astyanax mexicanus. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0119370"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1089\/zeb.2013.9999","article-title":"The CRISPR System\u2014Keeping Zebrafish Gene Targeting Fresh","volume":"10","author":"Blackburn","year":"2013","journal-title":"Zebrafish"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1089\/crispr.2020.0115","article-title":"Genome Editing Using the CRISPR-Cas9 System to Generate a Solid-Red Germline of Nile Tilapia (Oreochromis niloticus)","volume":"4","author":"Slosman","year":"2021","journal-title":"CRISPR J."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"2689","DOI":"10.1210\/me.2008-0290","article-title":"Proteomic Identification and Functional Validation of Activins and Bone Morphogenetic Protein 11 as Candidate Novel Muscle Mass Regulators","volume":"22","author":"Souza","year":"2008","journal-title":"Mol. Endocrinol."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1038\/387083a0","article-title":"Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member","volume":"387","author":"McPherron","year":"1997","journal-title":"Nature"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"9306","DOI":"10.1073\/pnas.151270098","article-title":"Regulation of myostatin activity and muscle growth","volume":"98","author":"Lee","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"7301","DOI":"10.1038\/s41598-017-07223-7","article-title":"Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR\/Cas9 System","volume":"7","author":"Khalil","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_107","unstructured":"Lilley, J.H., Callinan, R.B., Chinabut, S., Kanchanakhan, S., and MacRae, I.H. (1998). Epizootic Ulcerative Syndrome (EUS) Technical Handbook, The Aquatic Animal Health Research Institute AARI Department of Fisheries, Kasetsart University."},{"key":"ref_108","unstructured":"(2022, May 07). OIE (World Organisation for Animal Health) Listed Diseases. Available online: https:\/\/www.oie.int\/en\/what-we-do\/animal-health-and-welfare\/animal-diseases\/."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1007\/s11046-010-9386-2","article-title":"Fungal Proteases and Their Pathophysiological Effects","volume":"171","author":"Yike","year":"2011","journal-title":"Mycopathologia"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1186\/s13028-017-0347-3","article-title":"In vitro investigations on extracellular proteins secreted by Aphanomyces invadans, the causative agent of epizootic ulcerative syndrome","volume":"59","author":"Majeed","year":"2017","journal-title":"Acta Vet. Scand."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1186\/s13071-018-3134-8","article-title":"Editing the genome of Aphanomyces invadans using CRISPR\/Cas9","volume":"11","author":"Majeed","year":"2018","journal-title":"Parasites Vectors"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1111\/j.1365-2761.1993.tb01248.x","article-title":"Mycotic aspects of epizootic ulcerative syndrome (EUS) of Asian fishes","volume":"16","author":"Roberts","year":"1993","journal-title":"J. Fish Dis."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1111\/j.1439-0426.1994.tb00160.x","article-title":"Association of Aeromonas hydrophila with epizootic ulcerative syndrome (EUS) of freshwater fish in Sri Lanka","volume":"10","author":"Pathiratne","year":"1994","journal-title":"J. Appl. Ichthyol."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1046\/j.1365-2761.1997.d01-116.x","article-title":"Pathogenicity and culture studies comparing the Aphanomyces involved in epizootic ulcerative syndrome (EUS) with other similar fungi","volume":"20","author":"Lilley","year":"1997","journal-title":"J. Fish Dis."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0044-8486(98)00227-0","article-title":"Epizootic Ulcerative Syndrome (EUS), associated with a fungal pathogen, in Indian fishes: Histopathology\u2014\u2018A cause for invasiveness\u2019","volume":"165","author":"Vishwanath","year":"1998","journal-title":"Aquaculture"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/1297-9716-42-12","article-title":"The reverse genetics applied to fish RNA viruses","volume":"42","author":"Biacchesi","year":"2011","journal-title":"Vet. Res."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.fsi.2014.06.008","article-title":"RNA interference technology used for the study of aquatic virus infections","volume":"40","author":"Reshi","year":"2014","journal-title":"Fish Shellfish. Immunol."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/j.fsi.2012.11.037","article-title":"Exploring RNAi as a therapeutic strategy for controlling disease in aquaculture","volume":"34","author":"Lima","year":"2013","journal-title":"Fish Shellfish. Immunol."},{"key":"ref_119","first-page":"265","article-title":"Zebrafish as a genetic model organism","volume":"23","author":"Carpio","year":"2006","journal-title":"Biotecnol. Apl."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.molmed.2006.01.004","article-title":"MicroRNA therapeutics: A new niche for antisense nucleic acids","volume":"12","author":"Hammond","year":"2006","journal-title":"Trends Mol. Med."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1111\/jfd.12227","article-title":"Inhibition of spring viraemia of carp virus replication in an E pithelioma papulosum cyprini cell line by RNA i","volume":"38","author":"Gotesman","year":"2015","journal-title":"J. Fish Dis."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.jviromet.2014.05.022","article-title":"In vitro inhibition of Cyprinid herpesvirus-3 replication by RNAi","volume":"206","author":"Gotesman","year":"2014","journal-title":"J. Virol. Methods"},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"733","DOI":"10.2478\/pjvs-2014-0108","article-title":"Small interfering RNA treatment can inhibit Cyprinid herpesvirus 3 associated cell death in vitro","volume":"17","author":"Adamek","year":"2014","journal-title":"Pol. J. Vet. Sci."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1089\/nat.2016.0613","article-title":"In Vitro Gene Silencing of the Fish Microsporidian Heterosporis saurida by RNA Interference","volume":"26","author":"Saleh","year":"2016","journal-title":"Nucleic Acid Ther."},{"key":"ref_125","first-page":"7","article-title":"\u00dcber die Drehkrankheit der Regenbogenforelle","volume":"28","author":"Hofer","year":"1903","journal-title":"Allg Fisch-Ztg."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1089\/nat.2015.0549","article-title":"Can RNAi Target Salmonid Whirling Disease In Vivo?","volume":"25","author":"Sarker","year":"2015","journal-title":"Nucleic Acid Ther."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"155","DOI":"10.3354\/dao02856","article-title":"Whirling disease revisited: Pathogenesis, parasite biology and disease intervention","volume":"114","author":"Sarker","year":"2015","journal-title":"Dis. Aquat. Org."},{"key":"ref_128","doi-asserted-by":"crossref","unstructured":"Sarker, S., Menanteau-Ledouble, S., Kotob, M.H., and El-Matbouli, M. (2017). A RNAi-based therapeutic proof of concept targets salmonid whirling disease in vivo. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0178687"},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1089\/zeb.2012.0770","article-title":"Efficient shRNA-Mediated Inhibition of Gene Expression in Zebrafish","volume":"9","author":"Gutzman","year":"2012","journal-title":"Zebrafish"},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","article-title":"MicroRNAs: Target Recognition and Regulatory Functions","volume":"136","author":"Bartel","year":"2009","journal-title":"Cell"},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"101","DOI":"10.4161\/rna.2.3.2060","article-title":"Specific RNAi Mediated Gene Knockdown in Zebrafish Cell Lines","volume":"2","author":"Gruber","year":"2005","journal-title":"RNA Biol."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/j.funbio.2014.01.011","article-title":"Functional characterization of a tyrosinase gene from the oomycete Saprolegnia parasitica by RNAi silencing","volume":"118","author":"Saraiva","year":"2014","journal-title":"Fungal Biol."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"5196","DOI":"10.4049\/jimmunol.169.9.5196","article-title":"Inhibition of HIV-1 Infection by Small Interfering RNA-Mediated RNA Interference","volume":"169","author":"Capodici","year":"2002","journal-title":"J. Immunol."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1038\/35888","article-title":"Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans","volume":"391","author":"Fire","year":"1998","journal-title":"Nature"},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"2456","DOI":"10.1126\/science.1068836","article-title":"Systemic RNAi in C. elegans Requires the Putative Transmembrane Protein SID-1","volume":"295","author":"Winston","year":"2002","journal-title":"Science"},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1126\/science.1087117","article-title":"Transport of dsRNA into Cells by the Transmembrane Protein SID-1","volume":"301","author":"Feinberg","year":"2003","journal-title":"Science"},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"5932","DOI":"10.1016\/j.febslet.2005.08.001","article-title":"RNAi mechanisms in Caenorhabditis elegans","volume":"579","author":"Grishok","year":"2005","journal-title":"FEBS Lett."},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1023\/A:1018580301578","article-title":"Major viral diseases of the black tiger prawn (Penaeus monodon) in Thailand","volume":"13","author":"Flegel","year":"1997","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1577\/1548-8667(1998)010<0271:EIOWHP>2.0.CO;2","article-title":"Experimental infection of western hemisphere penaeid shrimp with asian white spot syndrome virus and asian yellow head virus","volume":"10","author":"Lightner","year":"1998","journal-title":"J. Aquat. Anim. Health"},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1128\/JVI.80.1.262-269.2006","article-title":"Identification and Characterization of a Penaeus monodon Lymphoid Cell-Expressed Receptor for the Yellow Head Virus","volume":"80","author":"Assavalapsakul","year":"2006","journal-title":"J. Virol."},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.virusres.2005.06.006","article-title":"siRNA injection induces sequence-independent protection in Penaeus monodon against white spot syndrome virus","volume":"114","author":"Westenberg","year":"2005","journal-title":"Virus Res."},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1007\/s00705-004-0483-8","article-title":"Nucleocapsid protein VP15 is the basic DNA binding protein of white spot syndrome virus of shrimp","volume":"150","author":"Witteveldt","year":"2005","journal-title":"Arch. Virol."},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1006\/viro.2001.0928","article-title":"White Spot Syndrome Virus Envelope Protein VP28 Is Involved in the Systemic Infection of Shrimp","volume":"285","author":"Witteveldt","year":"2001","journal-title":"Virology"},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"10365","DOI":"10.1128\/JVI.01101-06","article-title":"Antilipopolysaccharide Factor Interferes with White Spot Syndrome Virus Replication In Vitro and In Vivo in the Crayfish Pacifastacus leniusculus","volume":"80","author":"Liu","year":"2006","journal-title":"J. Virol."},{"key":"ref_145","doi-asserted-by":"crossref","first-page":"33593","DOI":"10.1074\/jbc.M706113200","article-title":"Phenoloxidase Is an Important Component of the Defense against Aeromonas hydrophila Infection in a Crustacean, Pacifastacus leniusculus","volume":"282","author":"Liu","year":"2007","journal-title":"J. Biol. Chem."},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/0016-6480(90)90112-Y","article-title":"Neuropeptides from the sinus gland of the lobster Homarus americanus: Characterization of hyperglycemic peptides","volume":"79","author":"Soyez","year":"1990","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_147","doi-asserted-by":"crossref","first-page":"5669","DOI":"10.1111\/j.1742-4658.2006.05555.x","article-title":"Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti: Functional analysis by double-stranded RNA interference technique","volume":"273","author":"Lugo","year":"2006","journal-title":"FEBS J."},{"key":"ref_148","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.ydbio.2006.07.024","article-title":"Knockdown of spalt function by RNAi causes de-repression of Hox genes and homeotic transformations in the crustacean Artemia franciscana","volume":"298","author":"Copf","year":"2006","journal-title":"Dev. Biol."},{"key":"ref_149","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1111\/j.1742-4658.2008.06266.x","article-title":"Molecular characterization of gonad-inhibiting hormone of Penaeus monodon and elucidation of its inhibitory role in vitellogenin expression by RNA interference","volume":"275","author":"Treerattrakool","year":"2008","journal-title":"FEBS J."},{"key":"ref_150","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1007\/BF01928162","article-title":"Crustacean neuropeptides: Structures, functions and comparative aspects","volume":"48","author":"Keller","year":"1992","journal-title":"Experientia"},{"key":"ref_151","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/0305-0491(95)00126-3","article-title":"Molecular biology of neurohormone precursors in the eyestalk of Crustacea","volume":"112","year":"1995","journal-title":"Comp. Biochem. Physiol. Part B Biochem. Mol. Biol."},{"key":"ref_152","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/S0016-6480(03)00263-6","article-title":"Crustacean neuropeptide genes of the CHH\/MIH\/GIH family: Implications from molecular studies","volume":"134","author":"Chan","year":"2003","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_153","doi-asserted-by":"crossref","first-page":"9049","DOI":"10.1093\/nar\/gkt555","article-title":"Optimization of scarless human stem cell genome editing","volume":"41","author":"Yang","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_154","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1038\/nbt.2623","article-title":"High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells","volume":"31","author":"Fu","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"ref_155","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1126\/science.1231143","article-title":"Multiplex Genome Engineering Using CRISPR\/Cas Systems","volume":"339","author":"Cong","year":"2013","journal-title":"Science"},{"key":"ref_156","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1038\/nbt.2647","article-title":"DNA targeting specificity of RNA-guided Cas9 nucleases","volume":"31","author":"Hsu","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"ref_157","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/522020a","article-title":"CRISPR, the disruptor","volume":"522","author":"Ledford","year":"2015","journal-title":"Nature"},{"key":"ref_158","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s40246-017-0116-4","article-title":"Falling giants and the rise of gene editing: Ethics, private interests and the public good","volume":"11","author":"Capps","year":"2017","journal-title":"Hum. Genom."},{"key":"ref_159","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1126\/science.1254287","article-title":"Regulating gene drives","volume":"345","author":"Oye","year":"2014","journal-title":"Science"},{"key":"ref_160","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s12919-018-0110-4","article-title":"Gene drives in our future: Challenges of and opportunities for using a self-sustaining technology in pest and vector management","volume":"12","author":"Collins","year":"2018","journal-title":"BMC Proc."},{"key":"ref_161","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1126\/science.aaa5945","article-title":"The mutagenic chain reaction: A method for converting heterozygous to homozygous mutations","volume":"348","author":"Gantz","year":"2015","journal-title":"Science"},{"key":"ref_162","doi-asserted-by":"crossref","first-page":"e03401","DOI":"10.7554\/eLife.03401","article-title":"Concerning RNA-guided gene drives for the alteration of wild populations","volume":"3","author":"Esvelt","year":"2014","journal-title":"eLife"},{"key":"ref_163","unstructured":"Hackett, P.B., Fahrenkrug, S.C., and Carlson, D.F. (2014). The Promises and Challenges of Precision Gene Editing in Animals of Agricultural Importance, eCommons. NABC, NABC report."},{"key":"ref_164","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.ygcen.2017.09.011","article-title":"Genome editing in fishes and their applications","volume":"257","author":"Zhu","year":"2018","journal-title":"Gen. Comp. Endocrinol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/7\/1250\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:09:53Z","timestamp":1760123393000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/7\/1250"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,4]]},"references-count":164,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["ani13071250"],"URL":"https:\/\/doi.org\/10.3390\/ani13071250","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,4]]}}}