{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T08:29:30Z","timestamp":1784622570511,"version":"3.55.0"},"reference-count":56,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,7]],"date-time":"2023-04-07T00:00:00Z","timestamp":1680825600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>In this paper, we show the effect of some essential oils (EOs) on staphylococci, including multidrug-resistant strains isolated from pyoderma in dogs. A total of 13 Staphylococcus pseudintermedius and 8 Staphylococcus aureus strains were studied. To assess the sensitivity of each strain to the antimicrobial agents, two commercial EOs from patchouli (Pogostemon cablin; PcEO) and tea tree (Melaleuca alternifolia; MaEO) as well as two antibiotics (gentamicin and enrofloxacin) were used. The minimum inhibitory concentration (MIC) followed by checkerboards in the combination of EO-antibiotic were performed. Finally, fractional inhibitory concentrations were calculated to determine possible interactions between these antimicrobial agents. PcEO MIC ranged from 0.125 to 0.5 % v\/v (1.2\u20134.8 mg\/mL), whereas MaEO MIC was tenfold higher (0.625\u20135% v\/v or 5.6\u201344.8 mg\/mL). Gentamicin appeared to be highly prone to interacting with EOs. Dual synergy (38.1% of cases) and PcEO additive\/MaEO synergism (53.4%) were predominantly observed. On the contrary, usually, no interactions between enrofloxacin and EOs were observed (57.1%). Both commercial EOs were characterized by natural composition without artificial adulteration. Patchouli and tea tree oils can be good alternatives for treating severe cases of pyoderma in dogs, especially when dealing with multidrug-resistant strains.<\/jats:p>","DOI":"10.3390\/ani13081279","type":"journal-article","created":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T03:15:57Z","timestamp":1681096557000},"page":"1279","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Activity of Patchouli and Tea Tree Essential Oils against Staphylococci Isolated from Pyoderma in Dogs and Their Synergistic Potential with Gentamicin and Enrofloxacin"],"prefix":"10.3390","volume":"13","author":[{"given":"Ma\u0142gorzata Anna","family":"Szewczuk","sequence":"first","affiliation":[{"name":"Department of Monogastric Animal Sciences, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology in Szczecin, 29 Klemensa Janickiego, 71-270 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u0142awomir","family":"Zych","sequence":"additional","affiliation":[{"name":"Laboratory of Chromatography and Mass Spectroscopy, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology in Szczecin, Klemensa Janickiego 29, 71-270 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Oster","sequence":"additional","affiliation":[{"name":"Department of Monogastric Animal Sciences, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology in Szczecin, 29 Klemensa Janickiego, 71-270 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jolanta","family":"Karakulska","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Biotechnology, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology in Szczecin, Piast\u00f3w 45, 70-311 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.tvjl.2018.04.002","article-title":"What has changed in canine pyoderma? 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