{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:57:23Z","timestamp":1792803443269,"version":"4.5.0"},"reference-count":68,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,9]],"date-time":"2023-04-09T00:00:00Z","timestamp":1680998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005285","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Goi\u00e1s (FAPEG)","doi-asserted-by":"publisher","award":["202110267000287"],"award-info":[{"award-number":["202110267000287"]}],"id":[{"id":"10.13039\/501100005285","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005285","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Goi\u00e1s (FAPEG)","doi-asserted-by":"publisher","award":["317557\/2021-1"],"award-info":[{"award-number":["317557\/2021-1"]}],"id":[{"id":"10.13039\/501100005285","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005285","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Goi\u00e1s (FAPEG)","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100005285","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["202110267000287"],"award-info":[{"award-number":["202110267000287"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["317557\/2021-1"],"award-info":[{"award-number":["317557\/2021-1"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014Brazil (CAPES)","award":["202110267000287"],"award-info":[{"award-number":["202110267000287"]}],"id":[{"id":"https:\/\/ror.org\/00x0ma614","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014Brazil (CAPES)","award":["317557\/2021-1"],"award-info":[{"award-number":["317557\/2021-1"]}],"id":[{"id":"https:\/\/ror.org\/00x0ma614","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014Brazil (CAPES)","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"https:\/\/ror.org\/00x0ma614","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Brazilian spotted fever (BSF) is the most important tick-borne diseases affecting humans in Brazil. Cases of BSF have recently been reported in the Goi\u00e1s state, midwestern Brazil. All cases have been confirmed by reference laboratories by seroconversion to Rickettsia rickettsii antigens. Because serological cross-reactions among different rickettsial species that belong to the spotted fever group (SFG) are common, the agent responsible for BSF cases in Goi\u00e1s remains unknown. From March 2020 to April 2022, ticks and plasma were collected from dogs, horses and capybaras (Hydrochoerus hydrochaeris), and from the vegetation in an area where BSF cases have been reported and two areas under epidemiological surveillance in Goi\u00e1s. Horses were infested by Amblyomma sculptum, Dermacentor nitens and Rhipicephalus microplus; dogs by Rhipicephalus sanguineus sensu lato (s.l.), Amblyomma ovale and A. sculptum, and capybaras by A. sculptum and Amblyomma dubitatum. Adults of A. sculptum, A. dubitatum, Amblyomma rotundatum and immature stages of A. sculptum and A. dubitatum, and Amblyomma spp. were collected from the vegetation. DNA of Rickettsia that did not belong to the SFG was detected in A. dubitatum, which was identified by DNA sequencing as Rickettsia bellii. Seroreactivity to SFG and Rickettsia bellii antigens was detected in 25.4% (42\/165) of dogs, 22.7% (10\/44) of horses and 41.2% (7\/17) of capybaras, with higher titers for R. bellii in dogs and capybaras. The seropositivity of animals to SFG Rickettsia spp. antigens demonstrates the circulation of SFG rickettsiae in the region. Further research is needed to fully determine the agent responsible for rickettsiosis cases in this area.<\/jats:p>","DOI":"10.3390\/ani13081288","type":"journal-article","created":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T03:15:57Z","timestamp":1681096557000},"page":"1288","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Detection of Rickettsia spp. in Animals and Ticks in Midwestern Brazil, Where Human Cases of Rickettsiosis Were Reported"],"prefix":"10.3390","volume":"13","author":[{"given":"Lucianne Cardoso","family":"Neves","sequence":"first","affiliation":[{"name":"Veterinary and Animal Science School, Federal University of Goi\u00e1s, Goi\u00e2nia 74605-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Warley Vieira de Freitas","family":"Paula","sequence":"additional","affiliation":[{"name":"Veterinary and Animal Science School, Federal University of Goi\u00e1s, Goi\u00e2nia 74605-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6058-0495","authenticated-orcid":false,"given":"Luiza Gabriella Ferreira","family":"de Paula","sequence":"additional","affiliation":[{"name":"Veterinary and Animal Science School, Federal University of Goi\u00e1s, Goi\u00e2nia 74605-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1107-3913","authenticated-orcid":false,"given":"Bianca Barbara Fonseca","family":"da Silva","sequence":"additional","affiliation":[{"name":"Veterinary and Animal Science School, Federal University of Goi\u00e1s, Goi\u00e2nia 74605-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah Alves","family":"Dias","sequence":"additional","affiliation":[{"name":"Veterinary and Animal Science School, Federal University of Goi\u00e1s, Goi\u00e2nia 74605-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda Gomes","family":"Pereira","sequence":"additional","affiliation":[{"name":"Veterinary and Animal Science School, Federal University of Goi\u00e1s, Goi\u00e2nia 74605-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno S\u00e9rgio Alves","family":"Silva","sequence":"additional","affiliation":[{"name":"Directorate of Surveillance in Zoonoses, Superintendence of Health Surveillance, Municipal Health Department, Goi\u00e2nia 74891-135, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5203-012X","authenticated-orcid":false,"given":"Anai\u00e1 da Paix\u00e3o","family":"Sev\u00e1","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Environmental Sciences, Santa Cruz State University, Ilh\u00e9us 45662-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filipe","family":"Dantas-Torres","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoparasitology, Department of Immunology, Aggeu Magalh\u00e3es Institute, Oswaldo Cruz Foundation (Fiocruz), Recife 50740-465, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9675-3132","authenticated-orcid":false,"given":"Marcelo B.","family":"Labruna","sequence":"additional","affiliation":[{"name":"Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3817-225X","authenticated-orcid":false,"given":"Felipe da Silva","family":"Krawczak","sequence":"additional","affiliation":[{"name":"Veterinary and Animal Science School, Federal University of Goi\u00e1s, Goi\u00e2nia 74605-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1111\/j.1749-6632.2009.04516.x","article-title":"Ecology of rickettsia in South America","volume":"1166","author":"Labruna","year":"2009","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_2","unstructured":"Brasil. Minist\u00e9rio da Sa\u00fade (2023, February 14). \u00d3bitos por Febre Maculosa. Brasil, Regi\u00f5es e Unidades Federadas (Infec\u00e7\u00e3o). 2007\u20132022. Sistema de Informa\u00e7\u00e3o de Agravos de Notifica\u00e7\u00e3o, Available online: https:\/\/www.gov.br\/saude\/pt-br\/assuntos\/saude-de-a-a-z\/f\/febre-maculosa\/situacao-epidemiologica\/obitos-por-febre-maculosa-brasil-regioes-e-unidades-federadas-infeccao-2007-2022\/view."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1196\/annals.1374.044","article-title":"Brazilian Spotted Fever: A case series from an endemic area in southeastern Brazil. Epidemiological aspects","volume":"1078","author":"Angerami","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"521","DOI":"10.3201\/eid1603.091338","article-title":"Novel Spotted Fever Group Rickettsiosis, Brazil","volume":"16","author":"Spolidorio","year":"2010","journal-title":"Emerg. Infect Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"275","DOI":"10.3201\/eid1702.100859","article-title":"Eschar-associated Spotted Fever Rickettsiosis, Bahia, Brazil","volume":"17","author":"Silva","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"551","DOI":"10.4269\/ajtmh.16-0192","article-title":"Case report: Rickettsia sp. strain Atlantic Rainforest infection in a patient from a spotted fever-endemic area in southern Brazil","volume":"95","author":"Krawczak","year":"2016","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1186\/s13071-019-3730-2","article-title":"A Human case of spotted fever caused by Rickettsia parkeri strain Atlantic Rainforest and its association to the tick Amblyomma ovale","volume":"12","author":"Martins","year":"2019","journal-title":"Parasites Vectors"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e07322020","DOI":"10.1590\/0037-8682-0732-2020","article-title":"Letter Rickettsioses in Brazil: Distinct diseases and new paradigms for epidemiological surveillance","volume":"54","author":"Krawczak","year":"2021","journal-title":"J. Braz. Soc. Trop. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"8505483","DOI":"10.1155\/2018\/8505483","article-title":"Genotypic Characterization of Rickettsia bellii Reveals Distinct Lineages in the United States and South America","volume":"2018","author":"Krawczak","year":"2018","journal-title":"Biomed Res. Int."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1128\/CMR.00032-13","article-title":"Update on Tick-Borne Rickettsioses around the World: A Geographic Approach","volume":"26","author":"Parola","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1016\/j.ttbdis.2014.07.003","article-title":"Experimental infection with Rickettsia rickettsii in an Amblyomma dubitatum tick colony, naturally infected by Rickettsia bellii","volume":"5","author":"Sakai","year":"2014","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1017\/S0031182012000546","article-title":"Epidemiology of Brazilian spotted fever in the Atlantic Forest, state of S\u00e3o Paulo, Brazil","volume":"139","author":"Ogrzewalska","year":"2012","journal-title":"Parasitology"},{"key":"ref_13","first-page":"27","article-title":"Ecology, biology and distribution of spotted-fever tick vectors in Brazil","volume":"3","author":"Pinter","year":"2013","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"265","DOI":"10.3201\/eid1102.040656","article-title":"Rickettsial Infection in Animals and Brazilian Spotted Fever Endemicity","volume":"11","author":"Sangioni","year":"2005","journal-title":"Emerg. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"93","DOI":"10.4269\/ajtmh.2004.71.93","article-title":"Prevalence of antibodies to Spotted Fever Group Rickettsiae in Humans and Domestic Animals in a Brazilian Spotted Fever-Endemic Area in the State of S\u00e3o Paulo, Brazil: Serologic Evidence for Infection by Rickettsia rickettsii and Another Spotted Fever Group Rickettsia","volume":"71","author":"Horta","year":"2004","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s10493-016-0070-1","article-title":"Ecology of a tick-borne spotted fever in southern Brazil","volume":"70","author":"Krawczak","year":"2016","journal-title":"Exp. Appl. Acarol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Luz, H.R., Costa, F.B., Benatti, H.R., Ramos, V.N., Serpa, M.C.A., Martins, T.F., Acosta, I.C.L., Ramirez, D.G., Mu\u00f1oz-Leal, S., and Ramirez-Hernandez, A. (2019). Epidemiology of capybara-associated Brazilian spotted fever. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007734"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1016\/j.ttbdis.2014.07.010","article-title":"Epidemiology of Rickettsia sp. strain Atlantic rainforest in a spotted fever-endemic area of southern Brazil","volume":"5","author":"Barbieri","year":"2014","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.vetpar.2008.12.010","article-title":"Experimental Infection of capybaras Hydrochoerus hydrochaeris by Rickettsia rickettsii and evaluation of the transmission of the infection to ticks Amblyomma cajennense","volume":"161","author":"Souza","year":"2009","journal-title":"Vet. Parasitol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"101463","DOI":"10.1016\/j.ttbdis.2020.101463","article-title":"Capybaras (Hydrochoerus hydrochaeris) as amplifying hosts of Rickettsia rickettsii to Amblyomma sculptum ticks: Evaluation during primary and subsequent exposures to R. rickettsii infection","volume":"11","author":"Uchoa","year":"2020","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_21","unstructured":"Brasil. Minist\u00e9rio da Sa\u00fade (2023, February 14). Casos Confirmados de Febre Maculosa. Brasil, Grandes Regi\u00f5es e Unidades Federadas (Infec\u00e7\u00e3o). 2007\u20132022. Sistema de Informa\u00e7\u00e3o de Agravos de Notifica\u00e7\u00e3o, Available online: https:\/\/www.gov.br\/saude\/pt-br\/assuntos\/saude-de-a-a-z\/f\/febre-maculosa\/situacao-epidemiologica\/casos-confirmados-de-febre-maculosa-brasil-grandes-regioes-e-unidades-federadas-infeccao-2007-2022\/view."},{"key":"ref_22","unstructured":"Brasil (2023, April 08). Minist\u00e9rio da Sa\u00fade\/SVS\u2014Sistema de Informa\u00e7\u00e3o de Agravos de Notifica\u00e7\u00e3o\u2014Sinan Net, Available online: http:\/\/tabnet.datasus.gov.br\/cgi\/tabcgi.exe?sinannet\/cnv\/febremaculosabr.def."},{"key":"ref_23","unstructured":"Brasil. Governo de Goi\u00e1s (2023, January 30). Clima, vegeta\u00e7\u00e3o e hidrografia de Goi\u00e2nia, Available online: https:\/\/www.goias.gov.br\/conheca-goias\/geografia.html."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1590\/1089-6891v17i334947","article-title":"Epidemiological survey of supposed spotted fever outbreak","volume":"17","author":"Martins","year":"2016","journal-title":"Cienc. Anim. Bras."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Neves, L.C., Sousa-paula, L.C.d., Dias, S.A., da Silva, B.B.F., Paula, W.V.d.F., de Paula, L.G.F., Pereira, B.G., P\u00e1dua, G.T., Borsanelli, A.C., and Cardoso, E.R.N. (2023). Detection of an Undescribed Babesia Sp. in Capybaras and Amblyomma Ticks in Central-Western Brazil. Animals, 13.","DOI":"10.3390\/ani13010094"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2141","DOI":"10.1590\/1678-4162-11697","article-title":"Detec\u00e7\u00e3o de anticorpos anti-Rickettsia rickettsii em c\u00e3es residentes em \u00e1rea negligenciada no munic\u00edpio de S\u00e3o Paulo, SP, Brasil","volume":"72","author":"Souza","year":"2020","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/s10493-021-00615-5","article-title":"Seasonal dynamics of Amblyomma sculptum in two areas of the Cerrado biome midwestern Brazil, where human cases of rickettsiosis have been reported","volume":"84","author":"Zeringota","year":"2021","journal-title":"Exp. Appl. Acarol."},{"key":"ref_28","unstructured":"Barros-Battesti, D.M., Arzua, M., and Bechara, G.H. (2006). Carrapatos de import\u00e2ncia m\u00e9dico-veterin\u00e1ria da Regi\u00e3o Neotropical: Um guia ilustrado para identifca\u00e7\u00e3o de esp\u00e9cies, Vox\/Instituto Butantan."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.ttbdis.2010.03.002","article-title":"Nymphs of the genus Amblyomma (Acari: Ixodidae) of Brazil: Descriptions, redescriptions, and identification key","volume":"1","author":"Martins","year":"2010","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.ttbdis.2013.11.004","article-title":"Reassessment of the taxonomic status of Amblyomma cajennense (Fabricius, 1787) with the description of three new species, Amblyomma tonelliae n. sp., Amblyomma interandinum n. sp. and Amblyomma patinoi n. sp., and reinstatement of Amblyomma mixtum Koch, 1844, and Amblyomma sculptum Berlese, 1888 (Ixodida: Ixodidae)","volume":"5","author":"Nava","year":"2014","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"101252","DOI":"10.1016\/j.ttbdis.2019.06.012","article-title":"Ticks (Ixodida: Argasidae, Ixodidae) of Brazil: Updated species checklist and taxonomic keys","volume":"10","author":"Martins","year":"2019","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Clifford, C.M., Anastos, G., and Elbl, A. (1961). The larval Ixodid ticks of the Eastern United States (Acarina-Ixodidae), Miscellaneous Publications of the Entomological Society of America.","DOI":"10.4182\/BHJB6050.2-1.3"},{"key":"ref_33","first-page":"82","article-title":"Isolation of Rickettsia rickettsii from the tick Amblyomma sculptum from a Brazilian spotted fever-endemic area in the Pampulha lake region, southeastern Brazil","volume":"8","author":"Labruna","year":"2017","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1196\/annals.1374.103","article-title":"Isolation of Rickettsia rickettsii and Rickettsia bellii in cell culture from the tick Amblyomma aureolatum in Brazil","volume":"1078","author":"Pinter","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1603\/0022-2585-41.6.1073","article-title":"Rickettsia bellii and Rickettsia amblyommii in Amblyomma ticks from the state of Rond\u00f4nia, Western Amazon, Brazil","volume":"41","author":"Labruna","year":"2004","journal-title":"J. Med. Entomol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1089\/vbz.2006.0621","article-title":"Prevalence of Rickettsia infection in dogs from the urban and rural areas of Monte Negro Municipality, Western Amazon, Brazil","volume":"7","author":"Labruna","year":"2007","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1128\/CVI.00325-10","article-title":"Correlation between serum and plasma antibody titers to mycobacterial antigens","volume":"18","author":"Siev","year":"2011","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_38","first-page":"15","article-title":"Comparative study between sero and plasma use for antibodies anti-using indirect immunofluorescence assay in dogs seen at the Londrina State University Veterinary Hospital, 1996","volume":"18","author":"Navarro","year":"1997","journal-title":"Semin. Ci\u00eanc. Agr\u00e1r."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1590\/S0074-02762008000700012","article-title":"Experimental infection of dogs with a Brazilian strain of Rickettsia rickettsii: Clinical and laboratory findings","volume":"103","author":"Piranda","year":"2008","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1186\/s13071-016-1784-y","article-title":"Experimental infection of horses with Rickettsia rickettsii","volume":"9","author":"Ueno","year":"2016","journal-title":"Parasites Vectors"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1590\/S0074-02762007000700003","article-title":"Rickettsial infection in five areas of the state of S\u00e3o Paulo, Brazil","volume":"102","author":"Horta","year":"2007","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1128\/JCM.42.1.90-98.2004","article-title":"Rickettsia Species infecting Amblyomma cooperi ticks from an area in the state of S\u00e3o Paulo, Brazil, where Brazilian spotted fever is endemic","volume":"42","author":"Labruna","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1590\/S0074-02762005000800004","article-title":"Detection of Rickettsia rickettsii in the tick Amblyomma cajennense in a new Brazilian spotted fever-endemic area in the state of Minas Gerais","volume":"100","author":"Guedes","year":"2005","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1111\/j.1365-2915.2011.00982.x","article-title":"Experimental infection of the tick Amblyomma cajennense, Cayenne tick, with Rickettsia rickettsii, the agent of Rocky Mountain spotted sever","volume":"26","author":"Soares","year":"2012","journal-title":"Med. Vet. Entomol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1576","DOI":"10.1128\/jb.173.5.1576-1589.1991","article-title":"Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes","volume":"173","author":"Regnery","year":"1991","journal-title":"J. Bacteriol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1007\/s004360050433","article-title":"Mitochondrial 16S rDNA sequences and phylogenetic relationships of species of Rhipicephalus and other tick genera among Metastriata (Acari: Ixodidae)","volume":"84","author":"Mangold","year":"1998","journal-title":"Parasitol. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"6196","DOI":"10.1073\/pnas.86.16.6196","article-title":"Dynamics of mitochondrial DNA evolution in animals: Amplification and sequencing with conserved primers","volume":"86","author":"Kocher","year":"1989","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1756-3305-7-7","article-title":"Rickettsial infection in Amblyomma cajennense ticks and capybaras (Hydrochoerus hydrochaeris) in a Brazilian spotted fever-endemic area","volume":"7","author":"Krawczak","year":"2014","journal-title":"Parasites Vectors"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1089\/vbz.2009.0250","article-title":"Experimental infection of Rhipicephalus sanguineus ticks with the bacterium Rickettsia rickettsii, using experimentally infected dogs","volume":"11","author":"Piranda","year":"2011","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e021419","DOI":"10.1590\/s1984-29612020018","article-title":"Serosurvey on rickettsiae of the spotted fever group and Rickettsia bellii among dogs in the state of Goi\u00e1s, Brazil","volume":"29","author":"Neves","year":"2020","journal-title":"Braz. J. Vet. Parasitol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.21897\/rmvz.282","article-title":"Rickettsioses in Latin America, Caribbean, Spain and Portugal","volume":"16","author":"Labruna","year":"2011","journal-title":"Rev. MVZ Cordoba."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1590\/S1984-29612015008","article-title":"A Serological and molecular survey of Babesia vogeli, Ehrlichia canis and Rickettsia spp. among dogs in the state of Maranh\u00e3o, northeastern Brazil","volume":"24","author":"Costa","year":"2015","journal-title":"Braz. J. Vet. Parasitol."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Souza, C.E., Camargo, L.B., Pinter, A., and Donalisio, M.R. (2016). High Seroprevalence for Rickettsia rickettsii in equines suggests risk of human infection in silent areas for the Brazilian spotted fever. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0153303"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0304-4017(01)00649-5","article-title":"Seasonal dynamics of ticks (Acari: Ixodidae) on horses in the state of S\u00e3o Paulo, Brazil","volume":"105","author":"Labruna","year":"2002","journal-title":"Vet. Parasitol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0304-4017(01)00387-9","article-title":"Risk factors to tick infestations and their occurrence on horses in the state of S\u00e3o Paulo, Brazil","volume":"97","author":"Labruna","year":"2001","journal-title":"Vet. Parasitol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"364","DOI":"10.7705\/biomedica.v27i3.199","article-title":"Rickettsial infection in capybaras (Hyrochoerus hydrochaeris) from S\u00e3o Paulo Brazil: Serological evidence for infection by Rickettsia bellii and Rickettsia parkeri","volume":"27","author":"Pacheco","year":"2007","journal-title":"Biomedica"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1089\/vbz.2019.2479","article-title":"The Dynamics of ticks and capybaras in a residential park area in southeastern Brazil: Implications for the risk of Rickettsia rickettsii infection","volume":"19","author":"Nunes","year":"2019","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"101360","DOI":"10.1016\/j.ttbdis.2019.101360","article-title":"Comparative survival of the engorged stages of Amblyomma dubitatum and Amblyomma sculptum in the laboratory: Implications for Brazilian spotted fever epidemiology","volume":"11","author":"Luz","year":"2020","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_59","first-page":"67","article-title":"Influence of microhabitat use and behavior of Amblyomma sculptum and Amblyomma dubitatum nymphs (Acari: Ixodidae) on human risk for tick exposure, with notes on Rickettsia infection","volume":"9","author":"Ramos","year":"2017","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/s10493-009-9331-6","article-title":"Hosts, distribution and genetic divergence (16S rDNA) of Amblyomma dubitatum (Acari: Ixodidae)","volume":"51","author":"Nava","year":"2010","journal-title":"Exp. Appl. Acarol."},{"key":"ref_61","unstructured":"Onofrio, V.C., Duarte, M.R., Labruna, M.B., and Barros-Battesti, D.M. (2002). Congresso Brasileiro de Parasitologia Veterin\u00e1ria, Col\u00e9gio Brasileiro de Parasitologia Veterin\u00e1ria."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/s10493-007-9096-8","article-title":"Tick fauna from two locations in the Brazilian savannah","volume":"43","author":"Santos","year":"2007","journal-title":"Exp. Appl. Acarol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1590\/S1984-29612011000400009","article-title":"Rickettsia species infecting Amblyomma ticks from an area endemic for Brazilian spotted fever in Brazil","volume":"20","author":"Guedes","year":"2011","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1017\/S0031182012002065","article-title":"In Vitro isolation from Amblyomma ovale (Acari: Ixodidae) and ecological aspects of the Atlantic rainforest Rickettsia, the causative agent of a novel spotted fever rickettsiosis in Brazil","volume":"140","author":"Spolidorio","year":"2013","journal-title":"Parasitology"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1093\/jmedent\/47.5.913","article-title":"Survey of ticks (Acari: Ixodidae) and their Rickettsia in an Atlantic rain forest reserve in the state of S\u00e3o Paulo, Brazil","volume":"47","author":"Sabatini","year":"2010","journal-title":"J. Med. Entomol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1111\/mve.12249","article-title":"Amblyomma sculptum: Genetic diversity and rickettsias in the Brazilian Cerrado biome","volume":"31","author":"Bitencourth","year":"2017","journal-title":"Med. Vet. Entomol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1111\/mve.12363","article-title":"Genetic diversity, population structure and rickettsias in Amblyomma ovale in areas of epidemiological interest for spotted fever in Brazil","volume":"33","author":"Bitencourth","year":"2019","journal-title":"Med. Vet. Entomol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1590\/S0037-86822009000300023","article-title":"Pesquisa de Rickettsia spp. em carrapatos Amblyomma cajennense e Amblyomma dubitatum no estado de S\u00e3o Paulo","volume":"42","author":"Pacheco","year":"2009","journal-title":"Rev. Soc. Bras. Med. Trop."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/8\/1288\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:12:42Z","timestamp":1760123562000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/8\/1288"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,9]]},"references-count":68,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["ani13081288"],"URL":"https:\/\/doi.org\/10.3390\/ani13081288","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,9]]}}}