{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T03:23:30Z","timestamp":1768447410057,"version":"3.49.0"},"reference-count":72,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,5,9]],"date-time":"2023-05-09T00:00:00Z","timestamp":1683590400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017637","name":"Wroclaw University of Environmental and Life Sciences","doi-asserted-by":"publisher","award":["B031\/0018\/20"],"award-info":[{"award-number":["B031\/0018\/20"]}],"id":[{"id":"10.13039\/501100017637","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017637","name":"Wroclaw University of Environmental and Life Sciences","doi-asserted-by":"publisher","award":["PPI\/APM\/2019\/1\/00044\/U\/00001"],"award-info":[{"award-number":["PPI\/APM\/2019\/1\/00044\/U\/00001"]}],"id":[{"id":"10.13039\/501100017637","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014434","name":"Polish National Agency for Academic Exchange","doi-asserted-by":"publisher","award":["B031\/0018\/20"],"award-info":[{"award-number":["B031\/0018\/20"]}],"id":[{"id":"10.13039\/501100014434","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014434","name":"Polish National Agency for Academic Exchange","doi-asserted-by":"publisher","award":["PPI\/APM\/2019\/1\/00044\/U\/00001"],"award-info":[{"award-number":["PPI\/APM\/2019\/1\/00044\/U\/00001"]}],"id":[{"id":"10.13039\/501100014434","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Even though the search for methods improving cryopreservation of canine spermatozoa led to an improvement of post-thaw quality, fertilizing results after insemination with frozen\u2013thawed semen are still not satisfying. In this study, we focused on modification of spermatozoa membrane fluidity and investigated whether kinematic parameters as assessed by computer-assisted semen analyzer (CASA) can be improved. The primary aim of our study was to investigate whether the use of cholesterol-loaded cyclodextrins (CLC; 0.5 mg, 1 mg, 2 mg) and 2-Hydroxypropyl-\u00df-cyclodextrin (HBCD; 1 mg) positively influence capacitation status as examined by tyrosinphosphorylation, cholesterol efflux and zona binding assay (ZBA) of spermatozoa. The use of 0.5 mg of CLC increased the percentage of motile, progressive and rapid spermatozoa compared to the control. Addition of HBCD decreased motility and progressive motility of spermatozoa and the population with rapid movement in comparison to the control. The percentage of live spermatozoa without efflux of cholesterol compared to the control was increased when extender with 0.5 mg of CLC was used. There was no change in capacitation status. The zona binding ability of spermatozoa was significantly lower in the group with 0.5 mg of CLC than in the control. In conclusion, these results suggest that improvement of kinematic parameters does not necessarily coincide with better zona pellucida binding ability of spermatozoa.<\/jats:p>","DOI":"10.3390\/ani13101580","type":"journal-article","created":{"date-parts":[[2023,5,9]],"date-time":"2023-05-09T03:17:56Z","timestamp":1683602276000},"page":"1580","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Does Better Post-Thaw Motility of Dog Sperm Frozen with CLC Mean Better Zona Pellucida Binding Ability?"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7182-6406","authenticated-orcid":false,"given":"Zuzanna","family":"Ligocka","sequence":"first","affiliation":[{"name":"Department of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Plac Grunwaldzki 49, 50-366 Wroc\u0142aw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9131-3552","authenticated-orcid":false,"given":"Agnieszka","family":"Partyka","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Plac Grunwaldzki 49, 50-366 Wroc\u0142aw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5676-7644","authenticated-orcid":false,"given":"Sabine","family":"Sch\u00e4fer-Somi","sequence":"additional","affiliation":[{"name":"Platform for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine Vienna, Veterin\u00e4rplatz 1, 1210 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7462-3524","authenticated-orcid":false,"given":"Anna","family":"Mucha","sequence":"additional","affiliation":[{"name":"Department of Genetics, Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, Ko\u017cuchowska 7, 51-631 Wroc\u0142aw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1056-0784","authenticated-orcid":false,"given":"Wojciech","family":"Ni\u017ca\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Plac Grunwaldzki 49, 50-366 Wroc\u0142aw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.theriogenology.2004.03.016","article-title":"Cryopreservation of Semen in the Dog: Use of Ultra-Freezers of \u2212152 \u00b0C as a Viable Alternative to Liquid Nitrogen","volume":"63","author":"Batista","year":"2005","journal-title":"Theriogenology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.anireprosci.2010.01.009","article-title":"Freezing Canine Sperm: Comparison of Semen Extenders Containing Equex\u00ae and LDL (Low Density Lipoproteins)","volume":"119","author":"Bencharif","year":"2010","journal-title":"Anim. 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