{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:30:54Z","timestamp":1787027454787,"version":"build-2736575974"},"reference-count":63,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T00:00:00Z","timestamp":1683676800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P 30061-B26"],"award-info":[{"award-number":["P 30061-B26"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Hypometabolism and hypothermia are common reactions of birds and mammals to cope with harsh winter conditions. In small mammals, the occurrence of hibernation and daily torpor is entrained by photoperiod, and the magnitude of hypometabolism and decrease of body temperature (Tb) is influenced by the dietary supply of essential polyunsaturated fatty acids. We investigated whether similar effects exist in a non-hibernating large mammal, the red deer (Cervus elaphus). We fed adult females with pellets enriched with either linoleic acid (LA) or \u03b1-linolenic acid (ALA) during alternating periods of ad libitum and restricted feeding in a cross-over experimental design. Further, we scrutinized the role of photoperiod for physiological and behavioral seasonal changes by manipulating the amount of circulating melatonin. The deer were equipped with data loggers recording heart rate, core and peripheral Tb, and locomotor activity. Further, we regularly weighed the animals and measured their daily intake of food pellets. All physiological and behavioral parameters measured varied seasonally, with amplitudes exacerbated by restricted feeding, but with only few and inconsistent effects of supplementation with LA or ALA. Administering melatonin around the summer solstice caused a change into the winter phenotype weeks ahead of time in all traits measured. We conclude that red deer reduce energy expenditure for thermoregulation upon short daylength, a reaction amplified by food restriction.<\/jats:p>","DOI":"10.3390\/ani13101600","type":"journal-article","created":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T01:37:09Z","timestamp":1683769029000},"page":"1600","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Influence of Photoperiod, Intake of Polyunsaturated Fatty Acids, and Food Availability on Seasonal Acclimatization in Red Deer (Cervus elaphus)"],"prefix":"10.3390","volume":"13","author":[{"given":"Kristina","family":"Gasch","sequence":"first","affiliation":[{"name":"Research Institute of Wildlife Ecology, Department of Interdisciplinary Life Science, University of Veterinary Medicine Vienna, 1160 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7061-0830","authenticated-orcid":false,"given":"Manuela","family":"Habe","sequence":"additional","affiliation":[{"name":"Research Institute of Wildlife Ecology, Department of Interdisciplinary Life Science, University of Veterinary Medicine Vienna, 1160 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie Sophie","family":"Krauss","sequence":"additional","affiliation":[{"name":"Research Institute of Wildlife Ecology, Department of Interdisciplinary Life Science, University of Veterinary Medicine Vienna, 1160 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Painer-Gigler","sequence":"additional","affiliation":[{"name":"Research Institute of Wildlife Ecology, Department of Interdisciplinary Life Science, University of Veterinary Medicine Vienna, 1160 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabrielle","family":"Stalder","sequence":"additional","affiliation":[{"name":"Research Institute of Wildlife Ecology, Department of Interdisciplinary Life Science, University of Veterinary Medicine Vienna, 1160 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6785-5685","authenticated-orcid":false,"given":"Walter","family":"Arnold","sequence":"additional","affiliation":[{"name":"Research Institute of Wildlife Ecology, Department of Interdisciplinary Life Science, University of Veterinary Medicine Vienna, 1160 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1098\/rsbl.2012.0764","article-title":"Congruent responses to weather variability in high arctic herbivores","volume":"8","author":"Stien","year":"2012","journal-title":"Biol. Lett."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1890\/09-1070.1","article-title":"Climate, snow, ice, crashes, and declines in populations of reindeer and caribou (Rangifer tarandus L.)","volume":"80","author":"Tyler","year":"2010","journal-title":"Ecol. Monogr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1086\/physzool.68.6.30163788","article-title":"Hibernation versus Daily Torpor in Mammals and Birds: Physiological Variables and Classification of Torpor Patterns","volume":"68","author":"Geiser","year":"1995","journal-title":"Physiol. Zool."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1152\/physiol.00047.2014","article-title":"Ecophysiology of Omega Fatty Acids: A Lid for Every Jar","volume":"30","author":"Arnold","year":"2015","journal-title":"Physiology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"s124","DOI":"10.1017\/S1751731119003240","article-title":"Review: Seasonal differences in the physiology of wild northern ruminants","volume":"14","author":"Arnold","year":"2020","journal-title":"Animal"},{"key":"ref_6","first-page":"2559","article-title":"Thyroid hormones correlate with field metabolic rate in ponies, Equus ferus caballus","volume":"219","author":"Brinkmann","year":"2016","journal-title":"J. Exp. Biol."},{"key":"ref_7","first-page":"4320","article-title":"Saving energy during hard times: Energetic adaptations of Shetland pony mares","volume":"217","author":"Brinkmann","year":"2014","journal-title":"J. Exp. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.2527\/jas.2006-298","article-title":"Heart rate measurements as an index of energy expenditure and energy balance in ruminants: A review","volume":"85","author":"Brosh","year":"2007","journal-title":"J. Anim. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1111\/j.0269-8463.2004.00821.x","article-title":"Measuring metabolic rate in the field: The pros and cons of the doubly labelled water and heart rate methods","volume":"18","author":"Butler","year":"2004","journal-title":"Funct. Ecol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1071\/AN21490","article-title":"Rumen temperature is a reliable proxy of core body temperature in sheep (Ovis aries)","volume":"62","author":"Vesterdorf","year":"2022","journal-title":"Anim. Prod. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.2527\/jas.2010-3051","article-title":"Rumen temperature change monitored with remote rumen temperature boluses after challenges with bovine viral diarrhea virus and Mannheimia haemolytica","volume":"89","author":"Krehbiel","year":"2011","journal-title":"J. Anim. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.jtherbio.2007.09.002","article-title":"Do changes in feed intake or ambient temperature cause changes in cattle rumen temperature relative to core temperature?","volume":"33","author":"Beatty","year":"2008","journal-title":"J. Therm. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1242\/jeb.052282","article-title":"Regulation of heart rate and rumen temperature in red deer: Effects of season and food intake","volume":"214","author":"Turbill","year":"2011","journal-title":"J. Exp. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4566","DOI":"10.1242\/jeb.02536","article-title":"Seasonal adjustment of energy budget in a large wild mammal, the Przewalski horse (Equus ferus przewalskii) II. Energy expenditure","volume":"209","author":"Arnold","year":"2006","journal-title":"J. Exp. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"R174","DOI":"10.1152\/ajpregu.00593.2002","article-title":"Nocturnal hypometabolism as an overwintering strategy of red deer (Cervus elaphus)","volume":"286","author":"Arnold","year":"2004","journal-title":"Am. J. Physiol. Reg. Int. Comp. Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"R277","DOI":"10.1152\/ajpregu.00084.2015","article-title":"Contrary seasonal changes of rates of nutrient uptake, organ mass, and voluntary food intake in red deer (Cervus elaphus)","volume":"309","author":"Arnold","year":"2015","journal-title":"Am. J. Physiol. Reg. Int. Comp. Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1079\/PNS19940060","article-title":"Photoperiod and the regulation of annual and circannual cycles of food intake","volume":"53","author":"Loudon","year":"1994","journal-title":"Proc. Nutr. Soc."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1677\/joe.0.1250241","article-title":"Effects of melatonin and a dopamine agonist and antagonist on seasonal changes in voluntary intake, reproductive activity and plasma concentrations of prolactin and tri-iodothyronine in red deer hinds","volume":"125","author":"Milne","year":"1990","journal-title":"J. Endocrinol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"216","DOI":"10.2307\/2389890","article-title":"The interaction of food availability and endogenous physiological cycles on the grazing ecology of red deer hinds (Cervus elaphus)","volume":"7","author":"Heydon","year":"1993","journal-title":"Funct. Ecol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1002\/jez.1402730103","article-title":"Manipulating melatonin in red deer (Cervus elaphus): Differences in the response to food restriction and lactation on the timing of the breeding season and prolactin-dependent pelage changes","volume":"273","author":"Heydon","year":"1995","journal-title":"J. Exp. Zool."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"R1086","DOI":"10.1152\/ajpregu.00128.2007","article-title":"Cold-induced alterations of phospholipid fatty acyl composition in brown adipose tissue mitochondria are independent of uncoupling protein-1","volume":"293","author":"Ocloo","year":"2007","journal-title":"Am. J. Physiol. Reg. Int. Comp. Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Arnold, W., Ruf, T., Frey-Roos, F., and Bruns, U. (2011). Diet-Independent Remodeling of Cellular Membranes Precedes Seasonally Changing Body Temperature in a Hibernator. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0018641"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1242\/jeb.027888","article-title":"Mimicking the natural doping of migrant sandpipers in sedentary quails: Effects of dietary n-3 fatty acids on muscle membranes and PPAR expression","volume":"212","author":"Nagahuedi","year":"2009","journal-title":"J. Exp. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1242\/jeb.02660","article-title":"Relationship between n-3 PUFA content and energy metabolism in the flight muscles of a migrating shorebird: Evidence for natural doping","volume":"210","author":"Maillet","year":"2007","journal-title":"J. Exp. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2686","DOI":"10.1242\/jeb.02299","article-title":"Performance-enhancing role of dietary fatty acids in a long-distance migrant shorebird: The semipalmated sandpiper","volume":"209","author":"Maillet","year":"2006","journal-title":"J. Exp. Biol."},{"key":"ref_26","first-page":"283","article-title":"Photo-neuroendocrine control of seasonal cycles in body weight, pelage growth and reproduction: Lessons from the HPD sheep model","volume":"119","author":"Lincoln","year":"1998","journal-title":"Comp. Biochem. Physiol. C"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1086\/672372","article-title":"Social dominance is associated with individual differences in heart rate and energetic response to food restriction in female red deer","volume":"86","author":"Turbill","year":"2013","journal-title":"Physiol. Biochem. Zool."},{"key":"ref_28","unstructured":"Gasch, K., Hykollari, A., Habe, M., Painer-Gigler, J., Stalder, G., and Arnold, W. (Biology, 2023). Photoperiod and intake of essential fatty acids influence the acyl composition of phospholipids and the activities of key metabolic enzymes: An experimental study with red deer (Cervus elaphus), Biology, in prep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1034\/j.1600-079X.2003.00022.x","article-title":"Seasonal changes in melatonin concentrations in female Iberian red deer (Cervus elaphus hispanicus)","volume":"34","author":"Zarazaga","year":"2003","journal-title":"J. Pineal Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/j.vaa.2019.10.011","article-title":"Cardiovascular effects of intravenous vatinoxan (MK-467) in medetomidine\u2013tiletamine\u2013zolazepam anaesthetised red deer (Cervus elaphus)","volume":"47","author":"Einwaller","year":"2020","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"14466","DOI":"10.1038\/s41598-018-32778-4","article-title":"Circadian rhythmicity persists through the Polar night and midnight sun in Svalbard reindeer","volume":"8","author":"Arnold","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1111\/j.1365-2435.2010.01806.x","article-title":"Hypometabolism and basking: The strategies of Alpine ibex to endure harsh over-wintering conditions","volume":"25","author":"Signer","year":"2011","journal-title":"Funct. Ecol."},{"key":"ref_33","unstructured":"R Core Team (2022). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing. Available online: https:\/\/www.R-project.org\/."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/1467-9868.00374","article-title":"Thin-plate regression splines","volume":"65","author":"Wood","year":"2003","journal-title":"J. R. Stat. Soc. B"},{"key":"ref_35","unstructured":"Christensen, R.H.B. (2022, November 20). Ordinal\u2014Regression Models for Ordinal Data. Available online: https:\/\/CRAN.R-project.org\/package=ordinal."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1071\/ZO11064","article-title":"Nutritional ecology of essential fatty acids: An evolutionary perspective","volume":"59","author":"Hulbert","year":"2012","journal-title":"Aust. J. Zool."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"R1182","DOI":"10.1152\/ajpregu.1994.266.4.R1182","article-title":"Energy and protein requirements for molt in the king penguin Aptenodytes patagonicus","volume":"266","author":"Cherel","year":"1994","journal-title":"Am. J. Physiol. Reg. Int. Comp. Physiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1086\/589547","article-title":"Increased Energy Expenditure but Decreased Stress Responsiveness during Molt","volume":"81","author":"Cyr","year":"2008","journal-title":"Physiol. Biochem. Zool."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1086\/physzool.65.6.30158276","article-title":"Energy requirements for molt in the kestrel Falco tinnunculus","volume":"65","author":"Dietz","year":"1992","journal-title":"Physiol. Zool."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1007\/s10336-012-0924-1","article-title":"Molt, feather growth rate and body condition of male and female Barn Swallows","volume":"154","author":"Saino","year":"2013","journal-title":"J. Ornithol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1093\/beheco\/10.5.592","article-title":"Male-caused failure of female reproduction and its adaptive value in alpine marmots (Marmota marmota)","volume":"10","author":"Arnold","year":"1999","journal-title":"Behav. Ecol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1111\/brv.12137","article-title":"Daily torpor and hibernation in birds and mammals","volume":"90","author":"Ruf","year":"2015","journal-title":"Biol. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"20200215","DOI":"10.1098\/rstb.2020.0215","article-title":"Determinants of heart rate in Svalbard reindeer reveal mechanisms of seasonal energy management","volume":"376","author":"Trondrud","year":"2021","journal-title":"Philos. Trans. R. Soc. B"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.1038\/s41598-020-58298-8","article-title":"On the interplay between hypothermia and reproduction in a high arctic ungulate","volume":"10","author":"Schmidt","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"107","DOI":"10.3389\/fevo.2020.00107","article-title":"Seasonal Hypometabolism in Female Moose","volume":"8","author":"Thiel","year":"2020","journal-title":"Front. Ecol. Evol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"4037","DOI":"10.1038\/s41598-019-40576-9","article-title":"Energy expenditure and body temperature variations in llamas living in the High Andes of Peru","volume":"9","author":"Riek","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"7600","DOI":"10.1038\/s41598-017-07946-7","article-title":"Seasonal changes in energy expenditure, body temperature and activity patterns in llamas (Lama glama)","volume":"7","author":"Riek","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1017\/S1751731117001392","article-title":"Long-term adaptation capacity of ponies: Effect of season and feed restriction on blood and physiological parameters","volume":"12","author":"Brinkmann","year":"2017","journal-title":"Animal"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1242\/jeb.064832","article-title":"Adaptation strategies to seasonal changes in environmental conditions of a domesticated horse breed, the Shetland pony (Equus ferus caballus)","volume":"215","author":"Brinkmann","year":"2012","journal-title":"J. Exp. Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"18310","DOI":"10.1038\/s41598-021-97825-z","article-title":"Atypical for northern ungulates, energy metabolism is lowest during summer in female wild boars (Sus scrofa)","volume":"11","author":"Ruf","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1134\/S0012496607040205","article-title":"Seasonal changes in the body temperature of medium-sized and large mammals in cold climate","volume":"415","author":"Anufriev","year":"2007","journal-title":"Dokl. Biol. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e2100707118","DOI":"10.1073\/pnas.2100707118","article-title":"Surviving winter on the Qinghai-Tibetan Plateau: Pikas suppress energy demands and exploit yak feces to survive winter","volume":"118","author":"Speakman","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"McClune, D.W., Kostka, B., Delahay, R.J., Montgomery, W.I., Marks, N.J., and Scantlebury, D.M. (2015). Winter Is Coming: Seasonal Variation in Resting Metabolic Rate of the European Badger (Meles meles). PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0135920"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1007\/s00300-005-0054-9","article-title":"Seasonal variations in basal metabolic rate, lower critical temperature and responses to temporary starvation in the arctic fox (Alopex lagopus) from Svalbard","volume":"29","author":"Fuglesteg","year":"2006","journal-title":"Polar Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.cbpa.2005.10.020","article-title":"Seasonal patterns in body temperature of free-living rock hyrax (Procavia capensis)","volume":"143","author":"Brown","year":"2006","journal-title":"Comp. Biochem. Physiol. A"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"jeb244744","DOI":"10.1242\/jeb.244744","article-title":"Individual response in body mass and basal metabolism to the risks of predation and starvation","volume":"226","author":"Broggi","year":"2023","journal-title":"J. Exp. Biol."},{"key":"ref_57","first-page":"20131846","article-title":"Nocturnal hypothermia impairs flight ability in birds: A cost of being cool","volume":"280","author":"Carr","year":"2013","journal-title":"Proc. R. Soc. Lond. B"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1093\/condor\/104.4.705","article-title":"Avian facultative hypothermic responses: A review","volume":"104","author":"McKechnie","year":"2002","journal-title":"Condor"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2142","DOI":"10.1038\/s41598-018-20655-z","article-title":"Free-living greylag geese adjust their heart rates and body core temperatures to season and reproductive context","volume":"8","author":"Wascher","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_60","first-page":"R837","article-title":"Regulation of metabolic rate in Svalbard and Norwegian reindeer","volume":"247","author":"Nilssen","year":"1984","journal-title":"Am. J. Physiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"656","DOI":"10.2307\/3809457","article-title":"Seasonal metabolic rhythms of captive female white-tailed deer: A reexamination","volume":"56","author":"Mautz","year":"1992","journal-title":"J. Wildl. Manag."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1046\/j.1365-201x.2000.00767.x","article-title":"Seasonal changes in heart rate and food intake in reindeer (Rangifer tarandus tarandus)","volume":"170","author":"Mesteig","year":"2000","journal-title":"Acta Physiol. Scand."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3782","DOI":"10.1210\/en.2005-0132","article-title":"Photorefractoriness in mammals: Dissociating a seasonal timer from the circadian-based photoperiod response","volume":"146","author":"Lincoln","year":"2005","journal-title":"Endocrinology"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/10\/1600\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:32:03Z","timestamp":1760124723000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/10\/1600"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,10]]},"references-count":63,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2023,5]]}},"alternative-id":["ani13101600"],"URL":"https:\/\/doi.org\/10.3390\/ani13101600","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,10]]}}}