{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T22:27:56Z","timestamp":1768343276493,"version":"3.49.0"},"reference-count":53,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T00:00:00Z","timestamp":1683849600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000199","name":"USDA\/ARS","doi-asserted-by":"publisher","award":["3091-32000-035-00D"],"award-info":[{"award-number":["3091-32000-035-00D"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000199","name":"USDA\/ARS","doi-asserted-by":"publisher","award":["58-3091-8-005"],"award-info":[{"award-number":["58-3091-8-005"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Vetagro ((CLS)","award":["3091-32000-035-00D"],"award-info":[{"award-number":["3091-32000-035-00D"]}]},{"name":"Vetagro ((CLS)","award":["58-3091-8-005"],"award-info":[{"award-number":["58-3091-8-005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Previously, the supplementation of a microencapsulated blend of organic acids and botanicals improved the health and performance of broiler breeders under non-challenged conditions. This study aimed to determine if the microencapsulated blend impacted dysbiosis and necrotic enteritis (NE) in broiler breeders. Day-of-hatch chicks were assigned to non-challenge and challenge groups, provided a basal diet supplemented with 0 or 500 g\/MT of the blend, and subjected to a laboratory model for NE. On d 20\u201321, jejunum\/ileum content were collected for microbiome sequencing (n = 10; V4 region of 16S rRNA gene). The experiment was repeated (n = 3), and data were analyzed in QIIME2 and R. Alpha and beta diversity, core microbiome, and compositional differences were determined (significance at p \u2264 0.05; Q \u2264 0.05). There was no difference between richness and evenness of those fed diets containing 0 and 500 g\/MT microencapsulated blend, but differences were seen between the non-challenged and challenged groups. Beta diversity of the 0 and 500 g\/MT non-challenged groups differed, but no differences existed between the NE-challenged groups. The core microbiome of those fed 500 g\/MT similarly consisted of Lactobacillus and Clostridiaceae. Furthermore, challenged birds fed diets containing 500 g\/MT had a higher abundance of significantly different phyla, namely, Actinobacteriota, Bacteroidota, and Verrucomicrobiota, than the 0 g\/MT challenged group. Dietary supplementation of a microencapsulated blend shifted the microbiome by supporting beneficial and core taxa.<\/jats:p>","DOI":"10.3390\/ani13101627","type":"journal-article","created":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T09:26:13Z","timestamp":1683883573000},"page":"1627","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Impact of a Blend of Microencapsulated Organic Acids and Botanicals on the Microbiome of Commercial Broiler Breeders under Clinical Necrotic Enteritis"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5517-5794","authenticated-orcid":false,"given":"Dana K.","family":"Dittoe","sequence":"first","affiliation":[{"name":"Animal Science Department, University of Wyoming, 1000 E University Ave., Laramie, WY 82071, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Casey N.","family":"Johnson","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, College Station, TX 77845, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"II","given":"James A.","family":"Byrd","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, College Station, TX 77845, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven C.","family":"Ricke","sequence":"additional","affiliation":[{"name":"College of Agricultural and Life Sciences, University of Wisconsin, 1933 Observatory Dr., Madison, WI 53706, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Piva","sequence":"additional","affiliation":[{"name":"DIMEVET, Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia, 40064 Bologna, Italy"},{"name":"Vetagro S.p.A., Via Porro 2, 42124 Reggio Emilia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ester","family":"Grilli","sequence":"additional","affiliation":[{"name":"DIMEVET, Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia, 40064 Bologna, Italy"},{"name":"Vetagro Inc., 17 East Monroe St. Suite 179, Chicago, IL 60603, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5057-8175","authenticated-orcid":false,"given":"Christina L.","family":"Swaggerty","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, College Station, TX 77845, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"557","DOI":"10.3382\/japr.2013-00925","article-title":"Necrotic enteritis: Applications for the poultry industry","volume":"23","author":"Paiva","year":"2014","journal-title":"J. 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