{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T23:13:11Z","timestamp":1780096391527,"version":"3.54.0"},"reference-count":25,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T00:00:00Z","timestamp":1684886400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Veterinary Clinic Rostock GmbH"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Background: The lung tissue in newborn canine neonates is still in a morphologically and functionally immature, canalicular\u2013saccular stage. True alveoli are only formed postnatally. The aim of this study was to analyze the spatial and temporal development of the ventilation of the lung tissue in vital canine neonates during the first 24 h post natum (p.n.). Methods: Forty pups (birth weight \u00d8 424 g \u00b1 80.1 g) from three litters of large dog breeds (&gt;20 kg live weight) were included in the studies. Thirty-three pups (29 vital, 2 vitally depressed, 2 stillborn neonates) originated from controlled, uncomplicated births (n = 3); moreover, six stillborn pups as well as one prematurely deceased pup were birthed by other dams with delivery complications. Computed tomography (CT) was used in 39 neonates, and histopathologic tissue classification techniques (HALO) were used in 11 neonates (eight stillborn and three neonates died early post natum, respectively) to quantify the degree of aerated neonatal lung tissue. Results: It was shown that, in vital born pups, within the first 10 min p.n., the degree of ventilation reached mean values of \u2212530 (\u00b1114) Hounsfield units (HU) in the dorsal and \u2212453.3 (\u00b1133) HU in the ventral lung area. This is about 75\u201380% of the final values obtained after 24 h p.n. for dorsal \u2212648.0 (\u00b189.9) HU and ventral quadrants \u2212624.7 (\u00b176.8) HU. The dorsal lung areas were always significantly better ventilated than the ventral regions (p = 0.0013). CT as well as histopathology are suitable to clearly distinguish the nonventilated lungs of stillborns from neonates that were initially alive after surviving neonatal respiratory distress syndrome but who died prematurely (p = 0.0398). Conclusion: The results of this study are clinically relevant since the lung tissue of canine neonates presents an aeration profile as early as 10 min after birth and continues progressively, with a special regard to the dorsal lung areas. This is the basis for resuscitation measures that should be performed, preferably with the pup in the abdomen\u2013chest position.<\/jats:p>","DOI":"10.3390\/ani13111741","type":"journal-article","created":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T01:36:28Z","timestamp":1684978588000},"page":"1741","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Computed Tomographic and Histopathologic Studies of Lung Function Immediately Post Natum in Canine Neonates"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7168-4970","authenticated-orcid":false,"given":"Jens Peter","family":"Teifke","sequence":"first","affiliation":[{"name":"Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia","family":"Peuckert","sequence":"additional","affiliation":[{"name":"Veterinary Clinic Rostock GmbH, 18059 Rostock, Germany"},{"name":"Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals with Veterinary Ambulance, Justus-Liebig-University, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens-Christian","family":"Rudnick","sequence":"additional","affiliation":[{"name":"Veterinary Clinic Rostock GmbH, 18059 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrin","family":"B\u00fcttner","sequence":"additional","affiliation":[{"name":"AG Biomathematics and Data Processing, Department of Veterinary Medicine, Justus-Liebig-University, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hartwig","family":"Bostedt","sequence":"additional","affiliation":[{"name":"Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals with Veterinary Ambulance, Justus-Liebig-University, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1002\/ar.1091040303","article-title":"The prenatal growth of the heart and the lungs in the dog","volume":"104","author":"Latimer","year":"1949","journal-title":"Anat. Rec."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1159\/000141809","article-title":"The postnatal growth of the lung in the dog","volume":"47","author":"Boyden","year":"1961","journal-title":"Acta Anat."},{"key":"ref_3","unstructured":"R\u00fcsse, I., and Sinowatz, F. (1991). Lehrbuch der Embryologie der Haustiere, Verlag Paul Parey."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Mortola, J.P. (2001). Respiratory Physiology of Newborn Mammals: A Comparative Perspective, Johns Hopkins University Press.","DOI":"10.1353\/book.21308"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"G\u00fcnzel-Apel, A.-R., and Bostedt, H. (2016). Reproduktionsmedizin und Neonatologie von Hund und Katze, Schattauer Verlag.","DOI":"10.1055\/b-005-148982"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.cvsm.2012.02.001","article-title":"Guide to emergency interception during parturition in the dog and cat","volume":"42","author":"Smith","year":"2012","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1055\/a-2056-0041","article-title":"Echtzeitanalyse des Geburtsverlaufes bei H\u00fcndinnen einer mittelgro\u00dfen Rasse","volume":"51","author":"Bostedt","year":"2023","journal-title":"Tier\u00e4rztl. Praxis Ausg. K."},{"key":"ref_8","first-page":"345","article-title":"Studies of factors influencing lung stability: Biochemical changes of pulmonary surfactant and morphological changes of terminal air spaces in the developing rat","volume":"48","author":"Suzuki","year":"1978","journal-title":"Jpn. J. Exp. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1007\/s00441-016-2545-0","article-title":"Development of the lung","volume":"367","author":"Schittny","year":"2017","journal-title":"Cell Tissue Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1213\/00000539-195301000-00041","article-title":"A proposal for a new method of evaluation of the newborn infant","volume":"32","author":"Apgar","year":"1953","journal-title":"Curr. Res. Anesth. Analg."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"157960","DOI":"10.1155\/2013\/157960","article-title":"Computer Tomographic Illustration of the Development of the Pulmonary Function in Bovine Neonates until the Twenty-First Day Postnatum","volume":"2013","author":"Linke","year":"2013","journal-title":"Vet. Med. Int."},{"key":"ref_12","first-page":"856","article-title":"On the influence of different factors on the frequency of losses in canine puppies (beagle breed)","volume":"80","author":"Hoy","year":"1999","journal-title":"Prakt. Tierarzt."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"946911","DOI":"10.3389\/fvets.2022.946911","article-title":"Thoracic Electrical Impedance Tomography-The 2022 Veterinary Consensus Statement","volume":"9","author":"Brabant","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"15","DOI":"10.15654\/TPK-150569","article-title":"Electrical impedance tomography for lung ventilation monitoring of the dog","volume":"45","author":"Gloning","year":"2017","journal-title":"Tier\u00e4rztl. Prax. Ausg. K."},{"key":"ref_15","unstructured":"Gaultier, C., Bourbon, J., and Post, M. (1999). Lung Disease, Oxford University Press."},{"key":"ref_16","first-page":"239","article-title":"Factors affecting pulmonary surface properties in the foetal lamb","volume":"29","author":"Howatt","year":"1965","journal-title":"Clin. Sci."},{"key":"ref_17","first-page":"92","article-title":"Fetal rhesus monkey lung development: Lobar differences and discordances between stability and distensibility","volume":"43","author":"Kotas","year":"1977","journal-title":"J. Appl. Physiol. Respir. Environ. Exerc. Physiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1172\/JCI105586","article-title":"Correlations of mechanical stability, morphology, pulmonary surfactant, and phospholipid content in the developing lamb lung","volume":"46","author":"Brumley","year":"1967","journal-title":"J. Clin. Investig."},{"key":"ref_19","first-page":"423","article-title":"Morphologic development of fetal rabbit lung and its acceleration with cortisol","volume":"64","author":"Kikkawa","year":"1971","journal-title":"Am. J. Pathol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1111\/j.1439-0531.2009.01446.x","article-title":"Pulmonary maturation in canine foetuses from early pregnancy to parturition","volume":"44","author":"Sipriani","year":"2009","journal-title":"Reprod. Domest. Anim."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.theriogenology.2020.05.005","article-title":"Morphometric and functional pulmonary changes of premature neonatal puppies after antenatal corticoid therapy","volume":"153","author":"Regazzi","year":"2020","journal-title":"Theriogenology"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Rieger, C., Hardt, H., von der Sennhauser, F.H., Wahn, U., and Zach, M. (2004). P\u00e4diatrische Pneumologie, Springer.","DOI":"10.1007\/978-3-662-09182-1"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/0002-9378(71)90342-5","article-title":"Diagnosis of the respiratory distress syndrome by amniocentesis","volume":"109","author":"Gluck","year":"1971","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_24","first-page":"1464","article-title":"Effects of hypoxia on ventilation during postnatal development in conscious kittens. J. Appl. Physiol. Respir. Environ","volume":"56","author":"Bonora","year":"1984","journal-title":"Exerc. Physiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S0034-5288(18)30665-9","article-title":"Lung surfactant composition in puppies dying of fading puppy complex","volume":"42","author":"Blunden","year":"1987","journal-title":"Res. Vet. Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/11\/1741\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:41:07Z","timestamp":1760125267000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/11\/1741"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,24]]},"references-count":25,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["ani13111741"],"URL":"https:\/\/doi.org\/10.3390\/ani13111741","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,24]]}}}