{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T02:50:49Z","timestamp":1791687049044,"version":"4.3.4"},"reference-count":63,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T00:00:00Z","timestamp":1687305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"PROCIENCIA\u2014CONCYTEC-Basic Research Project","award":["2019-01-Agreement No. 428-2019-FONDECYT"],"award-info":[{"award-number":["2019-01-Agreement No. 428-2019-FONDECYT"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Artificial insemination is an important assisted reproductive technology that has been applied in several mammalian species. However, successful cryopreservation of semen of South American camelids has been limited, hindering the commercial application of artificial insemination in alpaca species. In this scenario, the addition of antioxidants to semen extenders provides a strategy to improve the freezability of mammalian sperm. Bioactive metabolites from natural extracts of black maca have shown valuable antioxidant properties. Thus, the objective of this study was to evaluate the effect of the addition of atomized black maca in the freezing medium of epididymal spermatozoa of alpacas. Fifteen pairs of epididymis were collected from a local slaughterhouse. Each sample was divided into six groups: (1) fresh, (2) yolk medium (YM), (3) 10 mg\/mL maca, (4) 20 mg\/mL maca, (5) 30 mg\/mL maca, and (6) resveratrol (as an antioxidant control). Sperm cryopreservation was performed through the slow freezing method. Markers associated with functionality, such as motility, viability, and plasma membrane integrity, as well as markers associated with oxidative damage, such as DNA integrity, total ROS production, and mitochondrial function, were analyzed. The results show that the supplementation with black maca (20 mg\/mL) improved the sperm motility, viability, plasma membrane integrity, and mitochondrial function evaluated according to an index of formazan deposits. Similarly, the ROS production decreased with maca at 20 mg\/mL, although the DNA integrity did not show any differences among the groups. These results suggest that maca at 20 mg\/mL has cytoprotective effects during freezing\/thawing of epididymal sperm of alpaca species. Further research will be focused on assessing the effects of maca supplementation on semen extenders by using biomolecular markers (proAKAP4) associated with fertility.<\/jats:p>","DOI":"10.3390\/ani13132054","type":"journal-article","created":{"date-parts":[[2023,6,22]],"date-time":"2023-06-22T01:49:32Z","timestamp":1687398572000},"page":"2054","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effect of Atomized Black Maca (Lepidium meyenii) Supplementation in the Cryopreservation of Alpaca (Vicugna pacos) Epididymal Spermatozoa"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5400-6534","authenticated-orcid":false,"given":"Gloria","family":"Levano","sequence":"first","affiliation":[{"name":"Laboratory of Reproductive Physiology, Biological Sciences Faculty, Universidad Nacional Mayor de San Marcos, Lima 15081, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juana","family":"Quispe","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive Physiology, Biological Sciences Faculty, Universidad Nacional Mayor de San Marcos, Lima 15081, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6554-1292","authenticated-orcid":false,"given":"Diego","family":"Vargas","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive Physiology, Biological Sciences Faculty, Universidad Nacional Mayor de San Marcos, Lima 15081, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3028-3454","authenticated-orcid":false,"given":"Marlon","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Biological Sciences Faculty, Universidad Nacional Mayor de San Marcos, Lima 15081, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Biological Sciences Faculty, Universidad Nacional Mayor de San Marcos, Lima 15081, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9713-4248","authenticated-orcid":false,"given":"Luis","family":"Aguila","sequence":"additional","affiliation":[{"name":"Laboratory of Reproduction, Centre of Reproductive Biotechnology (CEBIOR-BIOREN), Universidad de La Frontera, Temuco 4811322, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8301-5536","authenticated-orcid":false,"given":"Martha","family":"Valdivia","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive Physiology, Biological Sciences Faculty, Universidad Nacional Mayor de San Marcos, Lima 15081, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,21]]},"reference":[{"key":"ref_1","unstructured":"Quina, E. (2017). Inseminaci\u00f3n Artificial de Alpacas en un Contexto de Crianza Campesina, Descosur."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Landeo, L., Zu\u00f1iga, M., Gastelu, T., Artica, M., Ruiz, J., Silva, M., and Ratto, M.H. (2022). Oocyte Quality, In Vitro Fertilization and Embryo Development of Alpaca Oocytes Collected by Ultrasound-Guided Follicular Aspiration or from Slaughterhouse Ovaries. Animals, 12.","DOI":"10.3390\/ani12091102"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1046\/j.1365-2605.2003.00404.x","article-title":"Participation of superoxide anion in the capacitation of cryopreserved bovine sperm","volume":"26","author":"Beorlegui","year":"2003","journal-title":"Int. J. Androl."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/j.1745-7262.2005.00021.x","article-title":"Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders","volume":"7","author":"Santiani","year":"2005","journal-title":"Asian J. Androl."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1111\/j.1439-0272.2011.01186.x","article-title":"Evaluation of Lama glama semen viscosity with a cone-plate rotational viscometer","volume":"44","author":"Casaretto","year":"2011","journal-title":"Andrologia"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1007\/s10815-019-01505-x","article-title":"Improved cryopreservation of spermatozoa using vitrification: Comparison of cryoprotectants and a novel device for long-term storage","volume":"36","author":"Nikoloska","year":"2019","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/j.theriogenology.2012.11.008","article-title":"Cryopreservation of rabbit semen: Comparing the effects of different cryoprotectants, cryoprotectant-free vitrification, and the use of albumin plus osmoprotectants on sperm survival and fertility after standard vapor freezing and vitrification","volume":"79","author":"Rosato","year":"2013","journal-title":"Theriogenology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/j.1600-0897.2007.00559.x","article-title":"REVIEW ARTICLE: Clinical Relevance of Oxidative Stress in Male Factor Infertility: An Update","volume":"59","author":"Agarwal","year":"2007","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1586\/17474108.3.4.539","article-title":"The impact of oxidative stress on in vitro fertilization","volume":"3","author":"Makker","year":"2008","journal-title":"Expert Rev. Obstet. Gynecol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1071\/RD15325","article-title":"Causes and consequences of oxidative stress in spermatozoa","volume":"28","author":"Aitken","year":"2016","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1002\/mrd.22871","article-title":"Reactive oxygen species as mediators of sperm capacitation and pathological damage","volume":"84","author":"Aitken","year":"2017","journal-title":"Mol. Reprod. Dev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.cryobiol.2018.06.001","article-title":"Cryopreservation induces mitochondrial permeability transition in a bovine sperm model","volume":"83","author":"Treulen","year":"2018","journal-title":"Cryobiology"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.anireprosci.2018.12.011","article-title":"Impact of post-thaw supplementation of semen extender with antioxidants on the quality and function variables of stallion spermatozoa","volume":"201","author":"Treulen","year":"2018","journal-title":"Anim. Reprod. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.theriogenology.2017.11.019","article-title":"Does MnTBAP ameliorate DNA fragmentation and in vivo fertility of frozen-thawed Arabian stallion sperm?","volume":"108","author":"Shojaeian","year":"2018","journal-title":"Theriogenology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1007\/s10561-016-9566-5","article-title":"The role of antioxidants in sperm freezing: A review","volume":"17","author":"Amidi","year":"2016","journal-title":"Cell Tissue Bank."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Ros-Santaella, J.L., and Pintus, E. (2021). Plant Extracts as Alternative Additives for Sperm Preservation. Antioxidants, 10.","DOI":"10.3390\/antiox10050772"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Pasquariello, R., Verdile, N., Brevini, T.A.L., Gandolfi, F., Boiti, C., Zerani, M., and Maranesi, M. (2020). The Role of Resveratrol in Mammalian Reproduction. Molecules, 25.","DOI":"10.20944\/preprints202009.0003.v1"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"5621","DOI":"10.1021\/jf020280x","article-title":"Investigation of the Tuber Constituents of Maca (Lepidium meyeniiWalp.)","volume":"50","author":"Piacente","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1677\/joe.0.1760163","article-title":"Effect of Lepidium meyenii (Maca), a root with aphrodisiac and fertility-enhancing properties, on serum reproductive hormone levels in adult healthy men","volume":"176","author":"Gonzales","year":"2003","journal-title":"J. Endocrinol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/S0090-4295(99)00549-X","article-title":"Effect of a lipidic extract from Lepidium meyenii on sexual behavior in mice and rats","volume":"55","author":"Zheng","year":"2000","journal-title":"Urology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/j.1439-0272.2008.00892.x","article-title":"Subjective effects of Lepidium meyenii (Maca) extract on well-being and sexual performances in patients with mild erectile dysfunction: A randomised, double-blind clinical trial","volume":"41","author":"Zenico","year":"2009","journal-title":"Andrologia"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1016\/j.jep.2005.08.035","article-title":"Effect of short-term and long-term treatments with three ecotypes of Lepidium meyenii (MACA) on spermatogenesis in rats","volume":"103","author":"Gonzales","year":"2006","journal-title":"J. Ethnopharmacol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S0308-8146(02)00133-4","article-title":"Antioxidant activity of the cruciferous vegetable Maca (Lepidium meyenii)","volume":"79","author":"Sandoval","year":"2002","journal-title":"Food Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.aquaculture.2004.12.006","article-title":"Activity-guided fractionation of phytochemicals of maca meal, their antioxidant activities and effects on growth, feed utilization, and survival in rainbow trout (Oncorhynchus mykiss) juveniles","volume":"244","author":"Lee","year":"2005","journal-title":"Aquaculture"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1016\/j.carbpol.2014.05.017","article-title":"Extraction, purification and antioxidant activities of the polysaccharides from maca (Lepidium meyenii)","volume":"111","author":"Zha","year":"2014","journal-title":"Carbohydr. Polym."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1111\/and.12682","article-title":"Effect of Lepidium meyenii(maca) on testicular function of mice with chemically and physically induced subfertility","volume":"48","author":"Valdivia","year":"2016","journal-title":"Andrologia"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1016\/j.fct.2006.01.007","article-title":"Lepidium meyenii (Maca) reversed the lead acetate induced-damage on reproductive function in male rats","volume":"44","author":"Rubio","year":"2006","journal-title":"Food Chem. Toxicol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1111\/j.1365-4632.2010.04793.x","article-title":"Photoprotection against the UVB-induced oxidative stress and epidermal damage in mice using leaves of three different varieties of Lepidium meyenii (maca)","volume":"50","author":"Rivera","year":"2011","journal-title":"Int. J. Dermatol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Dzi\u0119cio\u0142, M., Wr\u00f3blewska, A., and Janda-Milczarek, K. (2023). Comparative Studies of DPPH Radical Scavenging Activity and Content of Bioactive Compounds in Maca (Lepidium meyenii) Root Extracts Obtained by Various Techniques. Appl. Sci., 13.","DOI":"10.3390\/app13084827"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1111\/andr.12463","article-title":"Influences of dietary supplementation with Lepidium meyenii (Maca) on stallion sperm production and on preservation of sperm quality during storage at 5 \u00b0C","volume":"6","author":"Tafuri","year":"2018","journal-title":"Andrology"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Leiva-Revilla, J., Rol\u00f3n, M., Siyadatpanah, A., Pereira, M.D.L., and Nissapatorn, V. (2022). First study of in vitro protective effect of Lepidium meyenii (Maca) on frozen\u2013thawed bovine spermatozoa. Vet. World.","DOI":"10.14202\/vetworld.2022.1481-1488"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1002\/rmb2.12251","article-title":"Effect of Lepidium meyenii on in vitro fertilization via improvement in acrosome reaction and motility of mouse and human sperm","volume":"18","author":"Aoki","year":"2018","journal-title":"Reprod. Med. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Gonzales-Arimborgo, C., Yupanqui, I., Montero, E., Alarc\u00f3n-Yaquetto, D.E., Zevallos-Concha, A., Caballero, L., Gasco, M., Zhao, J., Khan, I.A., and Gonzales, G.F. (2016). Acceptability, Safety, and Efficacy of Oral Administration of Extracts of Black or Red Maca (Lepidium meyenii) in Adult Human Subjects: A Randomized, Double-Blind, Placebo-Controlled Study. Pharmaceuticals, 9.","DOI":"10.3390\/ph9030049"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1111\/rda.13151","article-title":"Follicular fluid stimulates capacitation and acrosome reaction in alpaca sperm (Vicugna pacos)","volume":"53","author":"Bravo","year":"2018","journal-title":"Reprod. Domest. Anim."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"434","DOI":"10.15381\/rivep.v26i3.11185","article-title":"Agentes crioprotectores alternativos para el congelamiento lento de espermatozoides epididimarios de alpaca (Vicugna pacos)","volume":"26","author":"Canorio","year":"2015","journal-title":"Rev. De Investig. Vet. Del Per\u00fa"},{"key":"ref_36","unstructured":"World Health Organization (WHO) (2021). Laboratory Manual for the Examination and Processing of Human Semen."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1530\/jrf.0.0700219","article-title":"Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics","volume":"70","author":"Jeyendran","year":"1984","journal-title":"Reproduction"},{"key":"ref_38","first-page":"182","article-title":"Evaluaci\u00f3n de la integridad de ADN mediante citometr\u00eda de flujo en espermatozoides de alpaca criopreservados con an\u00e1logos de super\u00f3xido dismutasa","volume":"23","author":"Santiani","year":"2012","journal-title":"Rev. De Investig. Vet. Del Per\u00fa"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Nova, Z., Skovierova, H., Strnadel, J., Halasova, E., and Calkovska, A. (2020). Short-Term versus Long-Term Culture of A549 Cells for Evaluating the Effects of Lipopolysaccharide on Oxidative Stress, Surfactant Proteins and Cathelicidin LL-37. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21031148"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1111\/j.1439-0272.1979.tb02217.x","article-title":"Cytochemistry of Oxidoreductases in Spermatozoa: The Technique Revisited","volume":"11","author":"Hrudka","year":"2009","journal-title":"Andrologia"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1002\/bem.22174","article-title":"Myrciaria dubia \u201ccamu camu\u201d flour as a magnetoprotector in male mouse infertility","volume":"40","author":"Torres","year":"2019","journal-title":"Bioelectromagnetics"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1071\/RD07049","article-title":"Cryopreservation of epididymal alpaca (Vicugna pacos) sperm: A comparison of citrate-, Tris- and lactose-based diluents and pellets and straws","volume":"19","author":"Morton","year":"2007","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1136\/vr.104074","article-title":"Semen collection methods in alpacas","volume":"180","author":"Abraham","year":"2017","journal-title":"Vet. Rec."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Tamburrino, L., Traini, G., Marcellini, A., Vignozzi, L., Baldi, E., and Marchiani, S. (2023). Cryopreservation of Human Spermatozoa: Functional, Molecular and Clinical Aspects. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24054656"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1111\/andr.12607","article-title":"A new system of sperm cryopreservation: Evaluation of survival, motility, DNA oxidation, and mitochondrial activity","volume":"7","author":"Meseguer","year":"2019","journal-title":"Andrology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1089\/bio.2018.0021","article-title":"Effect of Extender and Freezing Rate on Quality Parameters and In Vitro Fertilization Capacity of Alpaca Spermatozoa Recovered from Cauda Epididymis","volume":"17","author":"Gonzales","year":"2019","journal-title":"Biopreservation Biobanking"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.15381\/rivep.v30i3.16608","article-title":"Determinaci\u00f3n del porcentaje de viabilidad esperm\u00e1tica mediante citometr\u00eda de flujo durante el proceso de criopreservaci\u00f3n en espermatozoides obtenidos de epid\u00eddimo de alpaca","volume":"30","author":"Santiani","year":"2019","journal-title":"Rev. De Investig. Vet. Del Per\u00fa"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"597964","DOI":"10.3389\/fvets.2021.597964","article-title":"Rescue and Conservation of Male Adult Alpacas (Vicugna pacos) Based on Spermatogonial Stem Cell Biotechnology Using Atomized Black Maca as a Supplement of Cryopreservation Medium","volume":"8","author":"Valdivia","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.jevs.2018.10.006","article-title":"Freezing, Vitrification, and Freeze-Drying of Equine Spermatozoa: Impact on Mitochondrial Membrane Potential, Lipid Peroxidation, and DNA Integrity","volume":"72","author":"Restrepo","year":"2019","journal-title":"J. Equine Vet. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.theriogenology.2017.11.004","article-title":"Seasonal changes in the sperm fatty acid composition of Shetland pony stallions","volume":"107","author":"Aurich","year":"2018","journal-title":"Theriogenology"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Tafuri, S., Cocchia, N., Carotenuto, D., Vassetti, A., Staropoli, A., Mastellone, V., Peretti, V., Ciotola, F., Albarella, S., and Del Prete, C. (2019). Chemical Analysis of Lepidium meyenii (Maca) and Its Effects on Redox Status and on Reproductive Biology in Stallions. Molecules, 24.","DOI":"10.3390\/molecules24101981"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1111\/and.12679","article-title":"Effect of butanolic fraction of yellow and black maca (Lepidium meyenii) on the sperm count of adult mice","volume":"48","author":"Inoue","year":"2016","journal-title":"Andrologia"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/s11130-007-0042-z","article-title":"The Influence of Maca (Lepidium meyenii) on Antioxidant Status, Lipid and Glucose Metabolism in Rat","volume":"62","author":"Orolin","year":"2007","journal-title":"Plant Foods Hum. Nutr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.foodchem.2019.02.071","article-title":"Chemical composition and health effects of maca (Lepidium meyenii)","volume":"288","author":"Wang","year":"2019","journal-title":"Food Chem."},{"key":"ref_55","first-page":"301","article-title":"Lepidium meyenii (Maca) improved semen parameters in adult men","volume":"3","author":"Gonzales","year":"2001","journal-title":"Asian J. Androl."},{"key":"ref_56","first-page":"231","article-title":"Effect of Lepidium meyenii (maca) roots on spermatogenesis of male rats","volume":"3","author":"Gonzales","year":"2001","journal-title":"Asian J. Androl."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e12911","DOI":"10.1111\/and.12911","article-title":"Cyclophosphamide-induced male subfertility in mice: An assessment of the potential benefits of Maca supplement","volume":"50","author":"Onaolapo","year":"2018","journal-title":"Andrologia"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1177\/0960327110383625","article-title":"Resveratrol commonly displays hormesis: Occurrence and biomedical significance","volume":"29","author":"Calabrese","year":"2010","journal-title":"Hum. Exp. Toxicol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Ghaleno, L.R., Alizadeh, A., Drevet, J.R., Shahverdi, A., and Valojerdi, M.R. (2021). Oxidation of Sperm DNA and Male Infertility. Antioxidants, 10.","DOI":"10.3390\/antiox10010097"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"107074","DOI":"10.1016\/j.anireprosci.2022.107074","article-title":"ProAKAP4 protein marker: Towards a functional approach to male fertility","volume":"247","author":"Carracedo","year":"2022","journal-title":"Anim. Reprod. Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1530\/RAF-21-0055","article-title":"Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters","volume":"2","author":"Malo","year":"2021","journal-title":"Reprod. Fertil."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.cryobiol.2021.07.009","article-title":"First results about ProAKAP4 concentration in stallion semen after cryopreservation in two different freezing media","volume":"102","author":"Blommaert","year":"2021","journal-title":"Cryobiology"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Kowalczyk, A., Ga\u0142\u0119ska, E., and Bubel, A. (2022). The Concentration of ProAKAP4 and Other Indicators of Cryopotential of Spermatozoa Cryopreserved in Extender with Holothuroidea Extract Addition. Animals, 12.","DOI":"10.3390\/ani12040521"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/13\/2054\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:57:59Z","timestamp":1760126279000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/13\/2054"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,21]]},"references-count":63,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["ani13132054"],"URL":"https:\/\/doi.org\/10.3390\/ani13132054","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,21]]}}}