{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T01:08:05Z","timestamp":1775956085756,"version":"3.50.1"},"reference-count":46,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2023,6,22]],"date-time":"2023-06-22T00:00:00Z","timestamp":1687392000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FAPESP","award":["2016\/02384-9"],"award-info":[{"award-number":["2016\/02384-9"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Visceral canine leishmaniasis (CanL) can cause several clinical manifestations, including neurological lesions. Few reports have characterized the lesions observed in the central nervous system (CNS) during CanL; however, its pathogenesis remains unclear. The choroid plexus (CP) is a specialized structure responsible for the production and secretion of cerebrospinal fluid (CSF) and considered an interface between the peripheral immune system and CNS. It can allow the passage of inflammatory cells or pathogens and has the potential to act as a source of inflammatory mediators in several diseases. Thus, this study aimed to evaluate the role of CP as a possible route of inflammatory cells in the development of brain lesions in dogs with CanL, as well as its association with blood\u2013CSF barrier (BCSFB) dysfunction. Samples were collected from 19 dogs that were naturally infected with CanL. We evaluated the histopathological lesions in the brain and investigated the gene expression of the cytokines. Capture enzyme-linked immunosorbent assay (ELISA) was used to evaluate the presence of the same cytokines in the CSF. Biochemical analysis was performed to compare the presence of albumin in the serum and CSF. Indirect ELISA was performed to measure the presence of anti-Leishmania antibodies in the CSF, which would suggest the disruption of the BCSFB. Histopathological evaluation of the dogs\u2019 brains revealed mild-to-severe inflammatory infiltrates, mainly in the CP and meninges. We also detected the presence of anti-Leishmania antibodies and albumin in the CSF, as well as Leishmania DNA in the CP. The gene expression of CCL-5 was increased in the CP of infected dogs compared with that of controls, and there was a tendency for the increase in the gene expression of CXCL-10. Thus, our findings confirm the disfunction of the BCSFB during CanL and suggest that the chemokines CCL-5 and CXCL-10 can be responsible for the recruitment of inflammatory cells found in CP.<\/jats:p>","DOI":"10.3390\/ani13132060","type":"journal-article","created":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T01:54:56Z","timestamp":1687485296000},"page":"2060","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Increased CCL-5 (RANTES) Gene Expression in the Choroid Plexus of Dogs with Canine Leishmaniosis"],"prefix":"10.3390","volume":"13","author":[{"given":"Jos\u00e9 Eduardo dos Santos","family":"Silva","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine, Faculdade de Medicina Veterin\u00e1ria de Ara\u00e7atuba (FMVA), S\u00e3o Paulo State University (UNESP), R. Cl\u00f3vis Pestana, 793, Dona Am\u00e9lia, Ara\u00e7atuba 16050-680, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia Gon\u00e7alves","family":"Jussiani","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Faculdade de Medicina Veterin\u00e1ria de Ara\u00e7atuba (FMVA), S\u00e3o Paulo State University (UNESP), R. Cl\u00f3vis Pestana, 793, Dona Am\u00e9lia, Ara\u00e7atuba 16050-680, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda Grecco","family":"Grano","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Faculdade de Medicina Veterin\u00e1ria de Ara\u00e7atuba (FMVA), S\u00e3o Paulo State University (UNESP), R. Cl\u00f3vis Pestana, 793, Dona Am\u00e9lia, Ara\u00e7atuba 16050-680, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Cec\u00edlia Clarindo","family":"Pelissari","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Faculdade de Medicina Veterin\u00e1ria de Ara\u00e7atuba (FMVA), S\u00e3o Paulo State University (UNESP), R. Cl\u00f3vis Pestana, 793, Dona Am\u00e9lia, Ara\u00e7atuba 16050-680, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0747-7760","authenticated-orcid":false,"given":"Guilherme Dias","family":"de Melo","sequence":"additional","affiliation":[{"name":"Lyssavirus Epidemiology and Neuropathology Unit, Intitut Pasteur, Universit\u00e9 Paris Cit\u00e9, 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatiane Terumi","family":"Negr\u00e3o Watanabe","sequence":"additional","affiliation":[{"name":"Department Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA"},{"name":"Antech Diagnostics, 12401 West Olympic Blvd, Los Angeles, CA 90064, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0954-5045","authenticated-orcid":false,"given":"Val\u00e9ria Felix","family":"de Lima","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Faculdade de Medicina Veterin\u00e1ria de Ara\u00e7atuba (FMVA), S\u00e3o Paulo State University (UNESP), R. Cl\u00f3vis Pestana, 793, Dona Am\u00e9lia, Ara\u00e7atuba 16050-680, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gisele Fabrino","family":"Machado","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Faculdade de Medicina Veterin\u00e1ria de Ara\u00e7atuba (FMVA), S\u00e3o Paulo State University (UNESP), R. Cl\u00f3vis Pestana, 793, Dona Am\u00e9lia, Ara\u00e7atuba 16050-680, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/S1471-4922(02)02347-4","article-title":"Canine Leishmaniasis: Epidemiological Risk and the Experimental Model","volume":"18","author":"Moreno","year":"2002","journal-title":"Trends Parasitol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/1756-3305-4-86","article-title":"LeishVet Guidelines for the Practical Management of Canine Leishmaniosis","volume":"4","author":"Koutinas","year":"2011","journal-title":"Parasites Vectors"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/j.1748-5827.2004.tb00224.x","article-title":"Acute Paraplegia Associated with Vasculitis in a Dog with Leishmaniasis","volume":"45","author":"Font","year":"2004","journal-title":"J. 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