{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T10:43:54Z","timestamp":1769251434760,"version":"3.49.0"},"reference-count":32,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2023,6,25]],"date-time":"2023-06-25T00:00:00Z","timestamp":1687651200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Research and Development Project of Hainan Province, China","award":["ZDYF2021SHFZ261"],"award-info":[{"award-number":["ZDYF2021SHFZ261"]}]},{"name":"Key Research and Development Project of Hainan Province, China","award":["G2022034006L"],"award-info":[{"award-number":["G2022034006L"]}]},{"name":"Key Research and Development Project of Hainan Province, China","award":["320MS099"],"award-info":[{"award-number":["320MS099"]}]},{"name":"Key Research and Development Project of Hainan Province, China","award":["No. 1630032017038"],"award-info":[{"award-number":["No. 1630032017038"]}]},{"name":"State Foreign Expert Project from the Ministry of Science and Technology of the People\u2019s Republic of China","award":["ZDYF2021SHFZ261"],"award-info":[{"award-number":["ZDYF2021SHFZ261"]}]},{"name":"State Foreign Expert Project from the Ministry of Science and Technology of the People\u2019s Republic of China","award":["G2022034006L"],"award-info":[{"award-number":["G2022034006L"]}]},{"name":"State Foreign Expert Project from the Ministry of Science and Technology of the People\u2019s Republic of China","award":["320MS099"],"award-info":[{"award-number":["320MS099"]}]},{"name":"State Foreign Expert Project from the Ministry of Science and Technology of the People\u2019s Republic of China","award":["No. 1630032017038"],"award-info":[{"award-number":["No. 1630032017038"]}]},{"name":"Natural Science Found Project of Hainan Province, China","award":["ZDYF2021SHFZ261"],"award-info":[{"award-number":["ZDYF2021SHFZ261"]}]},{"name":"Natural Science Found Project of Hainan Province, China","award":["G2022034006L"],"award-info":[{"award-number":["G2022034006L"]}]},{"name":"Natural Science Found Project of Hainan Province, China","award":["320MS099"],"award-info":[{"award-number":["320MS099"]}]},{"name":"Natural Science Found Project of Hainan Province, China","award":["No. 1630032017038"],"award-info":[{"award-number":["No. 1630032017038"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences","award":["ZDYF2021SHFZ261"],"award-info":[{"award-number":["ZDYF2021SHFZ261"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences","award":["G2022034006L"],"award-info":[{"award-number":["G2022034006L"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences","award":["320MS099"],"award-info":[{"award-number":["320MS099"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences","award":["No. 1630032017038"],"award-info":[{"award-number":["No. 1630032017038"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>In the poultry industry, there is an urgent need to evaluate and introduce natural, effective, and safe alternatives for synthetic antibiotics, which have been banned in most countries. The present study aimed to investigate the effects of dietary supplementation with Piper sarmentosum extract (PSE) on the growth performance, intestinal barrier function, and metabolism of growing chickens. A total of 400 seven-day-old female chicks were randomly assigned to four dietary treatments, each of which consisted of five replicates and twenty birds each. The four experimental treatments were fed a basal diet containing 0, 100, 200, and 300 mg PSE\/kg (BC, PSE1, PSE2, and PSE3 groups), respectively. The experiment lasted for 28 days. The results showed that dietary supplementation with PSE had no significant effects on the final body weight, average daily gain (ADG), average daily feed intake (ADFI), and the ratio of ADFI to ADG (F\/G) (p &gt; 0.05). Compared with the BC group, dietary supplementation with 200\u2013300 mg\/kg PSE increased the villus height in the jejunum and ileum of chickens (p &lt; 0.05). The PSE-treated groups significantly increased the mRNA expression of Occludin, ZO-1, and Claudin-1 in the ileal mucosa of chickens (p &lt; 0.05). In addition, a significant decrease in ileal TNF-\u03b1 and IL-8 mRNA expression (p &lt; 0.05) and a significant increase in IL-22 (p &lt; 0.05) were observed in the PSE2 treatment compared to the BC group. Additionally, three gut metabolites (i.e., citrate, isocitrate, and spermine) showed significant differences among treatments (p &lt; 0.05) and were involved in the tricarboxylic acid (TCA) cycle, the transfer of acetyl groups into mitochondria, and spermidine and spermine biosynthesis, respectively. In conclusion, the findings obtained here indicate that supplemental PSE can enhance the anti-inflammatory capacity and intestinal mucosal barrier function of chickens.<\/jats:p>","DOI":"10.3390\/ani13132108","type":"journal-article","created":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T02:23:09Z","timestamp":1687746189000},"page":"2108","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Influence of Piper sarmentosum Extract on Growth Performance, Intestinal Barrier Function, and Metabolism of Growing Chickens"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5662-876X","authenticated-orcid":false,"given":"Luli","family":"Zhou","sequence":"first","affiliation":[{"name":"Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuhuan","family":"Lin","sequence":"additional","affiliation":[{"name":"Animal Health Supervision Institute of Hainan Province, Haikou 571100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Chang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6141-1095","authenticated-orcid":false,"given":"Khaled Fouad Mohammed","family":"Abouelezz","sequence":"additional","affiliation":[{"name":"Department of Poultry Production, Faculty of Agriculture, Assiut University, Assiut 71526, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6990-8792","authenticated-orcid":false,"given":"Hanlin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Zhanjiang Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guanyu","family":"Hou","sequence":"additional","affiliation":[{"name":"Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4189-6213","authenticated-orcid":false,"given":"Dingfa","family":"Wang","sequence":"additional","affiliation":[{"name":"Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4288","DOI":"10.3748\/wjg.v26.i29.4288","article-title":"Role of nutritional status and nutritional support in outcome of hepatitis B virus-associated acute-on-chronic liver failure","volume":"26","author":"Chang","year":"2020","journal-title":"World J. Gastroenterol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Abiala, M., Olayiwola, J., Babatunde, O., Aiyelaagbe, O., and Akinyemi, S. (2016). Evaluation of therapeutic potentials of plant extracts against poultry bacteria threatening public health. BMC Complement. Altern. Med., 16.","DOI":"10.1186\/s12906-016-1399-z"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.aninu.2020.12.004","article-title":"Natural capsicum extract replacing chlortetracycline enhances performance via improving digestive enzyme activities, antioxidant capacity, anti-inflammatory function, and gut health in weaned pigs","volume":"7","author":"Long","year":"2021","journal-title":"Anim. Nutr."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Stagos, D. (2019). Antioxidant activity of polyphenolic plant extracts. Antioxidants, 9.","DOI":"10.3390\/antiox9010019"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Wang, Y., Fei, Y., Liu, L., Xiao, Y., Pang, Y., Kang, J., and Wang, Z. (2018). Polygonatum odoratum polysaccharides modulate gut microbiota and mitigate experimentally induced obesity in rats. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19113587"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2767","DOI":"10.1002\/jsfa.10904","article-title":"Chemical composition and protective effect of guava (Psidium guajava L.) leaf extract on piglet intestines","volume":"101","author":"Wang","year":"2021","journal-title":"J. Sci. Food. Agric."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.3109\/13880209.2011.654229","article-title":"A review of the literature and latest advances in research of Piper sarmentosum","volume":"50","author":"Hussain","year":"2012","journal-title":"Pharm. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1080\/14786410801987597","article-title":"Antioxidant, anti-TB activities, phenolic and amide contents of standardised extracts of Piper sarmentosum Roxb","volume":"23","author":"Hussain","year":"2009","journal-title":"Nat. Prod. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2359","DOI":"10.17576\/jsm-2018-4710-12","article-title":"Piper sarmentosum as an antioxidant: A systematic review","volume":"47","author":"Ismail","year":"2018","journal-title":"Sains Malays."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/jpn.12517","article-title":"Effects of Piper sarmentosum extract on the growth performance, antioxidant capability and immune response in weaned piglets","volume":"101","author":"Wang","year":"2017","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"113723","DOI":"10.1016\/j.jep.2020.113723","article-title":"Chemical composition and anti-inflammatory activity of n-butanol extract of Piper sarmentosum Roxb. in the intestinal porcine epithelial cells (IPEC-J2)","volume":"269","author":"Wang","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"112897","DOI":"10.1016\/j.jep.2020.112897","article-title":"Piper sarmentosum Roxb.: A review on its botany, traditional uses, phytochemistry, and pharmacological activities","volume":"263","author":"Sun","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1007\/s11250-016-1034-5","article-title":"Anticoccidial effect of Piper sarmentosum extracts in experimental coccidiosis in broiler chickens","volume":"48","author":"Wang","year":"2016","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1007\/s11250-021-02934-6","article-title":"Dietary supplementation with Piper sarmentosum extracts on gut health of chickens infected with Eimeria tenella","volume":"53","author":"Memon","year":"2021","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_15","first-page":"1064","article-title":"Meat quality, colour stability, lipid and protein oxidation of broilers on diets supplemented with Piper sarmentosum leaves","volume":"53","author":"Pastsart","year":"2019","journal-title":"Indian J. Anim. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"656179","DOI":"10.3389\/fvets.2021.656179","article-title":"Effects of guava (Psidium guajava L.) leaf extract on growth performance and metabolomics in weaned piglets challenged by Escherichia coli","volume":"8","author":"Wang","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1016\/j.toxrep.2016.08.006","article-title":"C-Glycosylated flavonoids from black gram husk: Protection against DNA and erythrocytes from oxidative damage and their cytotoxic effect on HeLa cells","volume":"3","author":"Girish","year":"2016","journal-title":"Toxicol. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Ahmadifar, E., Pourmohammadi Fallah, H., Yousefi, M., Dawood, M.A.O., Hoseinifar, S.H., Adineh, H., Yilmaz, S., Paolucci, M., and Doan, H.V. (2021). The gene regulatory roles of herbal extracts on the growth, immune system, and reproduction of fish. Animals, 11.","DOI":"10.3390\/ani11082167"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"48","DOI":"10.3389\/fvets.2019.00048","article-title":"Dietary fiber and intestinal health of monogastric animals","volume":"6","author":"Jha","year":"2019","journal-title":"Front. Vet. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"9062","DOI":"10.1038\/s41598-021-88639-0","article-title":"Effects of Lactobacillus salivarius isolated from feces of fast-growing pigs on intestinal microbiota and morphology of suckling piglets","volume":"11","author":"Moturi","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"BSR20180077","DOI":"10.1042\/BSR20180077","article-title":"Bile salt dependent lipase promotes intestinal adaptation in rats with massive small bowel resection","volume":"38","author":"Yang","year":"2018","journal-title":"Biosci. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1017\/S1751731113000669","article-title":"Jejunal morphology and blood metabolites in tail biting, victim and control pigs","volume":"7","author":"Palander","year":"2013","journal-title":"Animal"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1038\/nri2653","article-title":"Intestinal mucosal barrier function in health and disease","volume":"9","author":"Turner","year":"2009","journal-title":"Nat. Rev. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1136\/gutjnl-2018-316906","article-title":"Tight junction proteins in gastrointestinal and liver disease","volume":"68","author":"Zeisel","year":"2019","journal-title":"Gut"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2710","DOI":"10.1091\/mbc.e14-02-0773","article-title":"Proinflammatory cytokine-induced tight junction remodeling through dynamic self-assembly of claudins","volume":"25","author":"Capaldo","year":"2014","journal-title":"Mol. Biol. Cell"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Zhu, Y., Liu, C., Chen, X., Lu, S., and Chen, J. (2020). Hepatoprotective effects and mechanisms of Ixeris denticulate water extract on liver cirrhosis in experimental rat. BMC Complement. Med. Ther., 20.","DOI":"10.1186\/s12906-020-02957-w"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2868","DOI":"10.1084\/jem.20171029","article-title":"ATG16L1 orchestrates interleukin-22 signaling in the intestinal epithelium via cGAS-STING","volume":"215","author":"Aden","year":"2018","journal-title":"J. Exp. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.sajb.2021.03.007","article-title":"Metabolic profiling of two medicinal Piper species","volume":"139","author":"Zhou","year":"2021","journal-title":"S. Afr. J. Bot."},{"key":"ref_30","first-page":"957","article-title":"Aconitate hydratase of mammals under oxidative stress","volume":"73","author":"Matasova","year":"2008","journal-title":"Biochemistry"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/s41467-019-13668-3","article-title":"Mitochondrial TCA cycle metabolites control physiology and disease","volume":"11","author":"Chandel","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1002\/iub.1237","article-title":"The function of spermine","volume":"66","author":"Pegg","year":"2014","journal-title":"IUBMB Life"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/13\/2108\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:00:42Z","timestamp":1760126442000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/13\/2108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,25]]},"references-count":32,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["ani13132108"],"URL":"https:\/\/doi.org\/10.3390\/ani13132108","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,25]]}}}