{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T12:28:01Z","timestamp":1768825681697,"version":"3.49.0"},"reference-count":98,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T00:00:00Z","timestamp":1688947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research on the Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory","award":["311021004"],"award-info":[{"award-number":["311021004"]}]},{"name":"Research on the Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory","award":["PM-zx555-202107-208"],"award-info":[{"award-number":["PM-zx555-202107-208"]}]},{"name":"Research on the Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory","award":["PMzx555-202106-195"],"award-info":[{"award-number":["PMzx555-202106-195"]}]},{"name":"Guangdong Special Environmental Fund","award":["311021004"],"award-info":[{"award-number":["311021004"]}]},{"name":"Guangdong Special Environmental Fund","award":["PM-zx555-202107-208"],"award-info":[{"award-number":["PM-zx555-202107-208"]}]},{"name":"Guangdong Special Environmental Fund","award":["PMzx555-202106-195"],"award-info":[{"award-number":["PMzx555-202106-195"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Due to the high meat yield and rich nutritional content, jade perch (Scortum barcoo) has become an important commercial aquaculture species in China. Jade perch has a slow growth rate, taking 3\u20134 years to reach sexual maturity, and has almost no difference in body size between males and females. However, the study of its gonad development and reproduction regulation is still blank, which limited the yield increase. Herein, the gonad transcriptomes of juvenile males and females of S. barcoo were identified for the first time. A total of 107,060 unigenes were successfully annotated. By comparing male and female gonad transcriptomes, a total of 23,849 differentially expressed genes (DEGs) were identified, of which 9517 were downregulated, and 14,332 were upregulated in the testis. In addition, a large number of DEGs involved in sex differentiation, gonadal development and differentiation and gametogenesis were identified, and the differential expression patterns of some genes were further verified using real-time fluorescence quantitative PCR. The results of this study will provide a valuable resource for further studies on sex determination and gonadal development of S. barcoo.<\/jats:p>","DOI":"10.3390\/ani13142254","type":"journal-article","created":{"date-parts":[[2023,7,11]],"date-time":"2023-07-11T01:42:58Z","timestamp":1689039778000},"page":"2254","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["De Novo Assembly, Characterization and Comparative Transcriptome Analysis of the Gonads of Jade Perch (Scortum barcoo)"],"prefix":"10.3390","volume":"13","author":[{"given":"Shiyan","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingying","family":"Lian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yikun","family":"Song","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinghua","family":"Chen","sequence":"additional","affiliation":[{"name":"South China Institute of Environmental Science, MEE, Guangzhou 510610, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianrong","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1080\/24701394.2018.1482283","article-title":"Phylogenetic analysis of sooty grunter and other major freshwater fishes in the suborder Percoidei based on mitochondrial DNA","volume":"30","author":"Zhao","year":"2019","journal-title":"Mitochondrial DNA Part A DNA Mapp. Seq. Anal."},{"key":"ref_2","first-page":"437","article-title":"New Report on Trichodiniasis (Protozoa: Ciliophora: Peritrichida) in Jade Perch; Scortum barcoo from Peninsular Malaysia","volume":"11","author":"Ihwan","year":"2016","journal-title":"Can. J. Fish. Aquat. Sci."},{"key":"ref_3","first-page":"282","article-title":"Extraction of omega-3 fatty acid from jade perch (Scortum barcoo) using enzymatic hydrolysis technique","volume":"20","author":"Iberahim","year":"2020","journal-title":"Ind. J. Chem."},{"key":"ref_4","first-page":"20190448","article-title":"Fine-scale behavioural adjustments of prey on a continuum of risk","volume":"286","author":"Kent","year":"2019","journal-title":"Proc. Biol. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1111\/j.1365-2109.2012.03093.x","article-title":"Effects of stocking density and food deprivation-related stress on the physiology and growth in adult Scortum barcoo (McCulloch & Waite)","volume":"44","author":"Luo","year":"2013","journal-title":"Aquac. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3346","DOI":"10.3109\/19401736.2015.1018215","article-title":"The complete mitochondrial genome of jade perch, Scortum barcoo (Perciformes: Terapontidae: Scortum)","volume":"27","author":"Liu","year":"2016","journal-title":"Mitochondrial DNA Part A DNA Mapp. Seq. Anal."},{"key":"ref_7","first-page":"258","article-title":"Sexual characteristic differences between male and female of jade perch Scortum barcoo","volume":"7","author":"Elhag","year":"2019","journal-title":"Int. J. Fish. Aquat. Stud."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"737587","DOI":"10.1016\/j.aquaculture.2021.737587","article-title":"Implications of genome-wide single nucleotide polymorphisms in jade perch (Scortum barcoo) reveals the putative XX\/XY sex-determination system, facilitating a new chapter of sex control in aquaculture","volume":"548","author":"Suntronpong","year":"2022","journal-title":"Aquaculture"},{"key":"ref_9","first-page":"23","article-title":"De lhermaphrodisme chez les vertebres","volume":"12","author":"Stephan","year":"1901","journal-title":"Ann. Fac. Sc. Marseille"},{"key":"ref_10","first-page":"1","article-title":"Rudimentary hermaphrodi tism in the symbranchoid eel","volume":"15","author":"Liu","year":"1944","journal-title":"Monopterus Javanensis Sin."},{"key":"ref_11","first-page":"313","article-title":"Preliminary observations and considerations on hermaphroditism and sexual differientiation of Sparus auratus","volume":"18","year":"1940","journal-title":"L. Publ. Staz. Zool. Nap."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1146\/annurev.genet.42.110807.091714","article-title":"Weird animal genomes and the evolution of vertebrate sex and sex chromosomes","volume":"42","year":"2008","journal-title":"Annu. Rev. Genet."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"329","DOI":"10.3390\/jmse3020329","article-title":"Sex control in fish: Approaches, challenges and opportunities for aquaculture","volume":"3","author":"Budd","year":"2015","journal-title":"J. Mar. Sci. Eng."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"962534","DOI":"10.3389\/fmars.2022.962534","article-title":"Comparative transcriptome analysis of the differentiating gonads in Scatophagus argus","volume":"9","author":"Mustapha","year":"2022","journal-title":"Front. Mar. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1534\/genetics.114.161158","article-title":"Genetics of sexual development: An evolutionary playground for fish","volume":"196","author":"Heule","year":"2014","journal-title":"Genetics"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.gene.2017.09.055","article-title":"Comparative transcriptome analysis of ovary and testis reveals potential sex-related genes and pathways in spotted knifejaw Oplegnathus punctatus","volume":"637","author":"Du","year":"2017","journal-title":"Gene"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1111\/j.1749-7345.2009.00249.x","article-title":"Effects of dietary lipids on growth and feed utilization of jade perch, Scortum barcoo","volume":"40","author":"Song","year":"2009","journal-title":"J. World Aquacult. Soc."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2840","DOI":"10.1111\/are.15135","article-title":"Effects of starvation stress on jade perch Scortum barcoo based on proteomics analysis","volume":"52","author":"Xie","year":"2021","journal-title":"Aquac. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1111\/are.14080","article-title":"Influence of continuous magnetic field exposure on water properties and subsequent effects on the growth performance, plasma biochemistry, nutritive value and liver histopathology of Jade Perch Scortum barcoo in a recirculating system","volume":"50","author":"Hassan","year":"2019","journal-title":"Aquac. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"114095","DOI":"10.1016\/j.ecoenv.2022.114095","article-title":"Transcriptome analysis of the spleen provides insight into the immunoregulation of Scortum barcoo under Streptococcus agalactiae infection","volume":"245","author":"Gao","year":"2022","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Han, C., Huang, W.W., Peng, S.H., Zhou, J.W., Zhan, H.W., Zhang, Y.Y., Li, W.J., Gong, J., and Li, Q. (2022). De Novo Assembly, Characterization and Comparative Transcriptome Analysis of the Mature Gonads in Spinibarbus hollandi. Animals, 13.","DOI":"10.3390\/ani13010166"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1500","DOI":"10.1007\/s00343-020-0251-y","article-title":"Identification of potential sex-related genes in Siniperca chuatsi","volume":"39","author":"Zhu","year":"2021","journal-title":"J. Oceanol. Limnol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Tao, W.J., Yuan, J., Zhou, L.Y., Sun, L.N., Sun, Y.L., Yang, S.J., Li, M.H., Zheng, S., Huang, B.F., and Wang, D.H. (2013). Characterization of gonadal transcriptomes from Nile tilapia (Oreochromis niloticus) reveals differentially expressed genes. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0063604"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.1007\/s10695-019-00693-8","article-title":"Comparative transcriptome analysis of male and female gonads reveals sexbiased genes in spotted scat (Scatophagus argus)","volume":"45","author":"He","year":"2019","journal-title":"Fish Physiol. Biochem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.3389\/fgene.2019.01128","article-title":"Transcriptome of gonads from high temperature induced sex reversal during sex determination and differentiation in Chinese tongue sole, Cynoglossus semilaevis","volume":"10","author":"Wang","year":"2019","journal-title":"Front. Genet."},{"key":"ref_26","first-page":"50","article-title":"Comparative RNA-Seq analysis of differentially expressed genes in the testis and ovary of Takifugu rubripes","volume":"22","author":"Wang","year":"2017","journal-title":"Comp. Biochem. Physiol. Part D Genom. Proteom."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Tian, C.X., Li, Z.Y., Dong, Z.D., Huang, Y., Du, T., Chen, H.P., Jiang, D.N., Deng, S.P., Zhang, Y.L., and Wanida, S. (2019). Transcriptome analysis of male and female mature gonads of silver sillago (Sillago sihama). Genes, 10.","DOI":"10.3390\/genes10020129"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Sun, F.Y., Liu, S.K., Gao, X.Y., Jiang, Y.L., Perera, D., Wang, X.L., Li, C., Sun, L.Y., Zhang, J.R., and Kaltenboeck, L. (2013). Male-biased genes in catfish as revealed by RNASeq analysis of the testis transcriptome. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0068452"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Chen, H.P., Li, Z.Y., Wang, Y.R., Huang, H., Yang, X.W., Li, S.F., Yang, W., and Li, G.L. (2021). Comparison of gonadal transcriptomes uncovers reproduction-related genes with sexually dimorphic expression patterns in Diodon hystrix. Animals, 11.","DOI":"10.3390\/ani11041042"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1038\/s41597-022-01523-y","article-title":"A chromosome-level genome assembly of the jade perch (Scortum barcoo)","volume":"9","author":"Lu","year":"2022","journal-title":"Sci. Data"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"Fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1038\/nprot.2013.084","article-title":"De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis","volume":"8","author":"Haas","year":"2013","journal-title":"Nat. Protoc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3210","DOI":"10.1093\/bioinformatics\/btv351","article-title":"BUSCO: Assessing genome assembly and annotation completeness with single-copy orthologs","volume":"31","author":"Simao","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3674","DOI":"10.1093\/bioinformatics\/bti610","article-title":"Blast2GO: A universal tool for annotation, visualization and analysis in functional genomics research","volume":"21","author":"Conesa","year":"2005","journal-title":"Bioinformatics"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie enables improved reconstruction of a transcriptome from RNA-seq reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1038\/nprot.2013.099","article-title":"Count-based differential expression analysis of RNA sequencing data using R and Bioconductor","volume":"8","author":"Anders","year":"2013","journal-title":"Nat. Protoc."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: A Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1089\/omi.2011.0118","article-title":"Cluster profiler: An R package for comparing biological themes among gene clusters","volume":"16","author":"Yu","year":"2012","journal-title":"Omics J. Integr. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.ygcen.2018.11.015","article-title":"Roles of estrogens in fish sexual plasticity and sex differentiation","volume":"277","author":"Li","year":"2019","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1530\/JOE-16-0551","article-title":"Blockage of androgen and administration of estrogen induce transdifferentiation of testis into ovary","volume":"233","author":"Shi","year":"2017","journal-title":"J. Endocrinol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1159\/000328823","article-title":"Dimorphic expression of various transcription factor and steroidogenic enzyme genes during gonadal ontogeny in the air-breathing catfish, Clarias gariepinus","volume":"5","author":"Raghuveer","year":"2011","journal-title":"Sex. Dev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.steroids.2015.06.011","article-title":"Steroids in teleost fishes: A functional point of view","volume":"103","author":"Tokarz","year":"2015","journal-title":"Steroids"},{"key":"ref_45","first-page":"233","article-title":"Steroidogenic acute regulatory protein: Structure, functioning, and regulation","volume":"84","author":"Tugaeva","year":"2019","journal-title":"Biochemistry"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"105974","DOI":"10.1016\/j.jsbmb.2021.105974","article-title":"The role of StAR2 gene in testicular differentiation and spermatogenesis in Nile tilapia (Oreochromis niloticus)","volume":"214","author":"Li","year":"2021","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1093\/biolre\/ioz132","article-title":"Steroidogenic acute regulatory protein and luteinizing hormone are required for normal ovarian steroidogenesis and oocyte maturation in zebrafish","volume":"101","author":"Shang","year":"2019","journal-title":"Biol. Reprod."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"291067","DOI":"10.1155\/2014\/291067","article-title":"Gonadal transcriptome analysis of male and female olive flounder (Paralichthys olivaceus)","volume":"2014","author":"Fan","year":"2014","journal-title":"Biomed. Res. Int."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/S0039-128X(96)00180-8","article-title":"17\u03b1, 20\u03b2-Dihydroxy-4-pregnen-3-one, a maturation-inducing hormone in fish oocytes: Mechanisms of synthesis and action","volume":"62","author":"Nagahama","year":"1997","journal-title":"Steroids"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"4282","DOI":"10.1210\/en.2007-0487","article-title":"A novel type of P450c17 lacking the lyase activity is responsible for C21-steroid biosynthesis in the fish ovary and head kidney","volume":"148","author":"Zhou","year":"2007","journal-title":"Endocrinology"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.ygcen.2014.11.010","article-title":"Dynamic expression of 11\u03b2-hydroxylase during testicular development, recrudescence and after hCG induction, in vivo and in vitro in catfish, Clarias batrachus","volume":"211","author":"Rajakumar","year":"2015","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1002\/mrd.10145","article-title":"Molecular cloning and expression during spermatogenesis of a cDNA encoding testicular 11beta-hydroxylase (P45011beta) in rainbow trout (Oncorhynchus mykiss)","volume":"62","author":"Kusakabe","year":"2002","journal-title":"Mol. Reprod. Dev."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1046\/j.1440-169X.1996.t01-2-00004.x","article-title":"Hormonal induction of all stages of spermatogenesis in germ-somatic cell coculture from immature Japanese eel testis","volume":"38","author":"Miura","year":"1996","journal-title":"Dev. Growth Differ."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0960-0760(00)00186-2","article-title":"Expression of cytochrome P45011\u03b2 (11\u03b2-hydroxylase) gene during gonadal sex differentiation and spermatogenesis in rainbow trout, Oncorhynchus mykiss","volume":"75","author":"Liu","year":"2000","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.aquatox.2004.10.017","article-title":"Effects of 4-tert-pentylphenol on the gene expression of P450 11\u03b2-hydroxylase in the gonad of medaka (Oryzias latipes)","volume":"71","author":"Yokota","year":"2005","journal-title":"Aquat. Toxicol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1095\/biolreprod.107.064246","article-title":"Sexual dimorphic expression of genes in gonads during early differentiation of a teleost fish, the Nile tilapia Oreochromis niloticus","volume":"78","author":"Ijiri","year":"2008","journal-title":"Biol. Reprod."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.ygcen.2008.08.008","article-title":"Gene expression profiling during spermatogenesis in early maturing male Atlantic salmon parr testes","volume":"159","author":"Maugars","year":"2008","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1002\/(SICI)1098-2795(199910)54:2<154::AID-MRD7>3.0.CO;2-5","article-title":"Involvement of estrogens in the process of sex differentiation in two fish species: The rainbow trout (Oncorhynchus mykiss) and a tilapia (Oreochromis niloticus)","volume":"54","author":"Guiguen","year":"1999","journal-title":"Mol. Reprod. Dev."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1677\/jme.0.0230167","article-title":"Suppression of P450 aromatase gene expression in sex-reversed males produced by rearing genetically female larvae at a high water temperature during a period of sex differentiation in the Japanese flounder (Paralichthys olivaceus)","volume":"23","author":"Kitano","year":"1999","journal-title":"J. Mol. Endocrinol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.mce.2017.07.013","article-title":"Ovarian aromatase loss-of-function mutant medaka undergo ovary degeneration and partial female-to-male sex reversal after puberty","volume":"460","author":"Nakamoto","year":"2018","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"37357","DOI":"10.1038\/srep37357","article-title":"Knockout of Zebrafish Ovarian Aromatase Gene (cyp19a1a) by TALEN and CRISPR\/Cas9 Leads to All-male Offspring Due to Failed Ovarian Differentiation","volume":"6","author":"Lau","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"3030","DOI":"10.1210\/en.2016-1865","article-title":"Targeted disruption of aromatase reveals dual functions of cyp19a1a during sex differentiation in zebrafish","volume":"158","author":"Yin","year":"2017","journal-title":"Endocrinology"},{"key":"ref_63","first-page":"2634","article-title":"Mutation of foxl2 or cyp19a1a results in female to male sex reversal in XX Nile tilapia","volume":"158","author":"Zhang","year":"2017","journal-title":"Endocrinology"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.cbpc.2010.02.014","article-title":"Efficacy of exemestane, a new generation of aromatase inhibitor, on sex differentiation in a gonochoristic fish","volume":"152","author":"Ruksana","year":"2010","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"ref_65","first-page":"94","article-title":"SF1 in the development of the adrenal gland and gonads","volume":"59","author":"Ozisik","year":"2003","journal-title":"Horm. Res."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1038\/ng.2890","article-title":"Whole-genome sequence of a flatfish provides insights into ZW sex chromosome evolution and adaptation to a benthic lifestyle","volume":"46","author":"Chen","year":"2014","journal-title":"Nat. Genet."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Wang, L., Sun, F., Wan, Z.Y., Yang, Z., Tay, Y.X., Lee, M., Ye, B., Wen, Y., Meng, Z., and Fan, B. (2022). Transposon-induced epigenetic silencing in the X chromosome as a novel form of dmrt1 expression regulation during sex determination in the fighting fish. BMC Biol., 20.","DOI":"10.1186\/s12915-021-01205-y"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1095\/biolreprod.107.062612","article-title":"The expression of nr0b1 and nr5a4 during gonad development and sex change in protandrous black porgy fish, Acanthopagrus schlegeli","volume":"78","author":"Wu","year":"2008","journal-title":"Biol. Reprod."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.cbpb.2019.04.002","article-title":"Expression and transcriptional regulation of gsdf in spotted scat (Scatophagus argus)","volume":"233","author":"Jiang","year":"2019","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1007\/s11427-018-9415-7","article-title":"Diverse and variable sex determination mechanisms in vertebrates","volume":"61","author":"Li","year":"2018","journal-title":"Sci. China Life Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1534\/genetics.117.300274","article-title":"Distinct and cooperative roles of amh and dmrt1 in self-renewal and differentiation of male germ cells in zebrafish","volume":"207","author":"Lin","year":"2017","journal-title":"Genetics"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"2955","DOI":"10.1073\/pnas.1018392109","article-title":"A Y-linked anti-M\u00fcllerian hormone duplication takes over a critical role in sex determination","volume":"109","author":"Hattori","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Li, M.H., Sun, Y.L., Zhao, J., Shi, H.J., Zeng, S., Ye, K., Jiang, D.N., Zhou, L.Y., Sun, L., and Tao, W.J. (2015). A Tandem Duplicate of Anti-M\u00fcllerian Hormone with a Missense SNP on the Y Chromosome Is Essential for Male Sex Determination in Nile Tilapia, Oreochromis niloticus. PLoS Genet., 11.","DOI":"10.1371\/journal.pgen.1005678"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Pan, Q.W., Feron, R., Yano, A., Guyomard, R., Jouanno, E., Vigouroux, E., Wen, M., Busnel, J.M., Bobe, J., and Concordet, J.P. (2019). Identification of the master sex determining gene in Northern pike (Esox lucius) reveals restricted sex chromosome differentiation. PLoS Genet., 15.","DOI":"10.1101\/549527"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1534\/genetics.111.137497","article-title":"Tracing the emergence of a novel sex-determining gene in medaka, Oryzias luzonensis","volume":"191","author":"Myosho","year":"2012","journal-title":"Genetics"},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Rondeau, E.B., Messmer, A.M., Sanderson, D.S., Jantzen, S.G., von Schalburg, K.R., Minkley, D.R., Leong, J.S., Macdonald, G.M., Davidsen, A.E., and Parker, W.A. (2013). Genomics of sablefish (Anoplopoma fimbria): Expressed genes, mitochondrial phylogeny, linkage map and identification of a putative sex gene. BMC Genom., 14.","DOI":"10.1186\/1471-2164-14-452"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.1111\/1755-0998.13369","article-title":"Chromosome level reference of Atlantic halibut Hippoglossus hippoglossus provides insight into the evolution of sexual determination systems","volume":"21","author":"Einfeldt","year":"2021","journal-title":"Mol. Ecol. Resour."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.ygcen.2011.09.011","article-title":"Bone morphogenetic protein 15 and growth differentiation factor 9 expression in the ovary of European sea bass (Dicentrarchus labrax): Cellular localization, developmental profiles, and response to unilateral ovariectomy","volume":"174","author":"Halm","year":"2011","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"113547","DOI":"10.1016\/j.ygcen.2020.113547","article-title":"Growth differentiation factor 9 (gdf9) and bone morphogenetic protein 15 (bmp15) are potential intraovarian regulators of steroidogenesis in Japanese flounder (Paralichthys olivaceus)","volume":"297","author":"Yu","year":"2020","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.ygcen.2011.01.009","article-title":"Exogenous GDF9 but not Activin A, BMP15 or TGF\u03b2 alters tight junction protein transcript abun-dance in zebrafish ovarian follicles","volume":"171","author":"Clelland","year":"2011","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1126\/science.1085222","article-title":"Conserved role of nanos proteins in germ cell development","volume":"301","author":"Tsuda","year":"2003","journal-title":"Science"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.gene.2018.11.078","article-title":"Expression pattern of nanos, piwil, dnd, vasa and pum genes during ontogenic development in Nile tilapia Oreochromis niloticus","volume":"688","author":"Jin","year":"2019","journal-title":"Gene"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"2238","DOI":"10.1093\/hmg\/ddr114","article-title":"NANOS3 function in human germ cell development","volume":"20","author":"Julaton","year":"2011","journal-title":"Hum. Mol. Genet."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.ydbio.2012.12.003","article-title":"nanos3 maintains germline stem cells and expression of the conserved germline stem cell gene nanos2 in the zebrafish ovary","volume":"374","author":"Beer","year":"2013","journal-title":"Dev. Biol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.cbpb.2018.01.002","article-title":"Nanos3 not nanos1 and nanos2 is a germ cell marker gene in large yellow croaker during embryogenesis","volume":"218","author":"Han","year":"2018","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1242\/dev.00316","article-title":"Dax1 regulates testis cord organization during gonadal differentiation","volume":"130","author":"Meeks","year":"2003","journal-title":"Development"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1038\/ng1141","article-title":"Dax1 is required for testis determination","volume":"34","author":"Meeks","year":"2003","journal-title":"Nat. Genet."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.modgep.2003.08.004","article-title":"Nr0b1 and its network partners are expressed early in murine embryos prior to steroidogenic axis organogenesis","volume":"4","author":"Clipsham","year":"2004","journal-title":"Gene Expr. Patterns"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.mce.2016.06.005","article-title":"nr0b1 (DAX1) mutation in zebrafish causes female-to-male sex reversal through abnormal gonadal proliferation and differentiation","volume":"433","author":"Chen","year":"2016","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1002\/mrd.20689","article-title":"Dax1 suppresses P450arom expression in medaka ovarian follicles","volume":"74","author":"Nakamoto","year":"2007","journal-title":"Mol. Reprod. Dev."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1016\/S0006-291X(02)02252-0","article-title":"Molecular cloning of DAX1 and SHP cDNAs and their expression patterns in the Nile tilapia, Oreochromis niloticus","volume":"297","author":"Wang","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"110644","DOI":"10.1016\/j.cbpb.2021.110644","article-title":"Characterization, expression, and regulatory effects of nr0b1a and nr0b1b in spotted scat (Scatophagus argus)","volume":"256","author":"Shi","year":"2021","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"ref_93","first-page":"1239","article-title":"Genome-wide identification, phylogeny, and gonadal expression of fox genes in Nile tilapia, Oreochromis niloticus","volume":"40","author":"Yuan","year":"2014","journal-title":"Fish Physiol. Biochem."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1242\/dev.125.17.3389","article-title":"Zebrafish Paraxial protocadherin is a downstream target of spadetail involved in morphogenesis of gastrula mesoderm","volume":"125","author":"Yamamoto","year":"1998","journal-title":"Development"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1016\/S0960-9822(00)00669-2","article-title":"The zebrafish forkhead transcription factor FoxH1\/Fast1 is a modulator of nodal signaling required for organizer formation","volume":"10","author":"Pogoda","year":"2000","journal-title":"Curr. Biol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1093\/biolre\/ioz225","article-title":"Homozygous mutation of foxh1 arrests oogenesis causing infertility in female Nile tilapia","volume":"102","author":"Tao","year":"2020","journal-title":"Biol. Reprod."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"623","DOI":"10.2108\/zsj.14.623","article-title":"Effect of micropylar morphology and size on rapid spermentry into the eggs of the medaka","volume":"14","author":"Iwamatsu","year":"2009","journal-title":"Zool. Sci."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1111\/jai.12586","article-title":"Molecular characterization of the cDNAs of two zona pellucida genes in the Chinese sturgeon, Acipenser sinensis Gray, 1835","volume":"30","author":"Yue","year":"2014","journal-title":"J. Appl. Ichthyol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/14\/2254\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:09:46Z","timestamp":1760126986000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/14\/2254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,10]]},"references-count":98,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["ani13142254"],"URL":"https:\/\/doi.org\/10.3390\/ani13142254","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,10]]}}}