{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T20:42:23Z","timestamp":1791924143757,"version":"4.3.4"},"reference-count":77,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T00:00:00Z","timestamp":1689811200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Bari \u201cAldo Moro\u201d","award":["DOT1302781"],"award-info":[{"award-number":["DOT1302781"]}]},{"name":"University of Bari \u201cAldo Moro\u201d","award":["1305\/2013"],"award-info":[{"award-number":["1305\/2013"]}]},{"name":"Rural Development Program (PSR)","award":["DOT1302781"],"award-info":[{"award-number":["DOT1302781"]}]},{"name":"Rural Development Program (PSR)","award":["1305\/2013"],"award-info":[{"award-number":["1305\/2013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Gentile di Puglia (GdP) is an autochthonous sheep breed of Southern Italy included among ovine breeds threatened by genetic erosion and extinction risk, which have been given attention by local and international institutions, thus emphasizing the need for germplasm conservation actions. In the present study, two assisted reproduction approaches, finalized for GdP conservation, were performed: (1) on-farm reproductive efficiency evaluation, expressed as pregnancy rate (PR), twin pregnancy rate (tPR), and body condition score (BCS), for three consecutive breeding cycles and (2) pre-pubertal lambs\u2019 immature cumulus\u2013oocyte complex (COC) retrieval, vitrification, in vitro maturation (IVM), and assessment of meiotic stage and bioenergetic-oxidative status compared with those of other Italian and European commercial breeds. PR and tPR were progressively reduced over time. In all clinical examination times, BCS was significantly lower in nonpregnant ewes compared with pregnant ones. Fresh GdP pre-pubertal lamb COCs achieved meiotic maturation and showed healthy bioenergetic\u2013oxidative status after IVM. Vitrification reduced the oocyte maturation rate in all groups. However, mature oocytes retained their cytoplasmic maturity, expressed as a mitochondria distribution pattern and activity, indicating promising developmental competence. In conclusion, clinical- and biotechnological-assisted reproduction approaches can support conservation strategies of GdP and other local sheep breeds in Southern Italy.<\/jats:p>","DOI":"10.3390\/ani13142371","type":"journal-article","created":{"date-parts":[[2023,7,21]],"date-time":"2023-07-21T01:52:35Z","timestamp":1689904355000},"page":"2371","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["New Strategies for Conservation of Gentile di Puglia Sheep Breed, an Autochthonous Capital of Millennial Tradition in Southern Italy"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5673-6111","authenticated-orcid":false,"given":"Letizia","family":"Temerario","sequence":"first","affiliation":[{"name":"Department of Biosciences, Biotechnologies & Environment, University of Bari Aldo Moro, Strada per Casamassima km 3, Valenzano, 70010 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davide","family":"Monaco","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Bari Aldo Moro, Strada per Casamassima km 3, Valenzano, 70010 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7136-4029","authenticated-orcid":false,"given":"Antonella","family":"Mastrorocco","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Biotechnologies & Environment, University of Bari Aldo Moro, Strada per Casamassima km 3, Valenzano, 70010 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6853-2757","authenticated-orcid":false,"given":"Nicola Antonio","family":"Martino","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Biotechnologies & Environment, University of Bari Aldo Moro, Strada per Casamassima km 3, Valenzano, 70010 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e1ndor","family":"Cseh","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Food Animal Medicine Clinic, University of Veterinary Medicine, Istv\u00e1n St. 2, 1078 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9109-3561","authenticated-orcid":false,"given":"Giovanni Michele","family":"Lacalandra","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Bari Aldo Moro, Strada per Casamassima km 3, Valenzano, 70010 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5673-0975","authenticated-orcid":false,"given":"Elena","family":"Ciani","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Biotechnologies & Environment, University of Bari Aldo Moro, Strada per Casamassima km 3, Valenzano, 70010 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Elena","family":"Dell\u2019Aquila","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Biotechnologies & Environment, University of Bari Aldo Moro, Strada per Casamassima km 3, Valenzano, 70010 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1111\/j.1439-0531.2012.02085.x","article-title":"How developments in cryobiology, reproductive technologies and conservation genomics could shape gene banking strategies for (farm) animals","volume":"47","author":"Woelders","year":"2012","journal-title":"Reprod. 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