{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T12:38:22Z","timestamp":1778589502968,"version":"3.51.4"},"reference-count":30,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T00:00:00Z","timestamp":1690329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Few studies have established the normal radiographic anatomical development of the donkey foal and, to date, no data are available for mules. Our aim was thus to evaluate the radiographic development of the fore digit and carpal joint in the mule foal from 0 to 3 months of age. Ten forelimbs of five healthy full-term mule foals were included. Radiographs of the fore digit lateromedial and dorsopalmar and the carpus dorsopalmar were performed weekly for the first month of age, and bi-monthly during the following two months. Fore digit growth plate closure times, morphological, angular, and linear radiographic parameters, and also carpal cuboidal bone mineralization were evaluated. Growth plates were graded as open, closing, and closed. Carpal bone appearance was graded as mature, slightly immature, or immature. Growth plate closure times showed the following: middle phalangeal distal physis (DP) closed at birth, and proximal physis (PP) started to close at around two months; first phalanx DP closed at seven days, and PP started to close at three months; and third metacarpal bone DP started to close at two months. Carpal bones were immature at birth. Distal phalanx (PD) was triangular at birth, palmar processes had developed by one month, and proximal sesamoid bones were trapezoidal by three months. The hoof wall, PD dorsal wall, and palmar angles values changed in parallel, and hoof capsule thickness increased. No PD remodeling evidence within the hoof capsule or variations in the hoof axis with growth were found. We identified a baseline for the interpretation of forelimb radiological features in mule foals. The specific features found were intermediate between those found in horses and donkeys.<\/jats:p>","DOI":"10.3390\/ani13152417","type":"journal-article","created":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T01:50:37Z","timestamp":1690422637000},"page":"2417","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Radiographic Appearance of the Fore Digit and Carpal Joint in the Mule Foal from Birth to 3 Months of Age"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9536-4453","authenticated-orcid":false,"given":"Irene","family":"Nocera","sequence":"first","affiliation":[{"name":"Institute of Life Sciences, Sant\u2019Anna School of Advanced Studies, Via Santa Cecilia 3, 56127 Pisa, Italy"},{"name":"Department of Veterinary Sciences, Veterinary Teaching Hospital \u201cMario Modenato\u201d, Via Livornese snc, 56122 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benedetta","family":"Sorvillo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, Veterinary Teaching Hospital \u201cMario Modenato\u201d, Via Livornese snc, 56122 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0294-1803","authenticated-orcid":false,"given":"Micaela","family":"Sgorbini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, Veterinary Teaching Hospital \u201cMario Modenato\u201d, Via Livornese snc, 56122 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benedetta","family":"Aliboni","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, Veterinary Teaching Hospital \u201cMario Modenato\u201d, Via Livornese snc, 56122 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8211-9248","authenticated-orcid":false,"given":"Simonetta","family":"Citi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, Veterinary Teaching Hospital \u201cMario Modenato\u201d, Via Livornese snc, 56122 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.cveq.2019.08.002","article-title":"Clinical pathology of donkeys and mules","volume":"35","author":"Goodrich","year":"2019","journal-title":"Vet. Clin. Equine Pract."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.cveq.2019.08.003","article-title":"Genetics, evolution, and physiology of donkeys and mules","volume":"35","author":"Brosnahan","year":"2019","journal-title":"Vet. Clin. N. Am. Equine Pract."},{"key":"ref_3","first-page":"31","article-title":"Some morphological characteristics of mules raised in Van Province in Turkey","volume":"23","author":"Yilmaz","year":"2013","journal-title":"Yuz. Yil Univ. J. Agric. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/j.jevs.2015.03.112","article-title":"Measuring conformation in mules, hinnies, and donkeys (Equus asinus) from Spanish and Portuguese populations","volume":"5","author":"McLean","year":"2015","journal-title":"J. Equine Vet. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Hanot, P., Herrel, A., Guintard, C., and Cornette, R. (2019). Unravelling the hybrid vigor in domestic equids: The effect of hybridization on bone shape variation and covariation. BMC Evol. Biol., 19.","DOI":"10.1186\/s12862-019-1520-2"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/jbg.12180","article-title":"Genetic relationships among American donkey populations: Insights into the process of colonization","volume":"133","author":"Jordana","year":"2016","journal-title":"J. Anim. Breed. Genet."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/scientificamerican1270-102","article-title":"The mule","volume":"223","author":"Savory","year":"1970","journal-title":"Sci. Am."},{"key":"ref_8","unstructured":"Butler, J.A., Colles, C.M., Dyson, S.J., Kold, S.E., and Poulos, P.W. (2017). Clinical Radiology of the Horse, John Wiley & Sons. [3rd ed.]."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1111\/j.2042-3306.2010.00312.x","article-title":"Radiological anatomy of the donkey foot: Objective characterization of the normal and laminitic donkey foot","volume":"43","author":"Collins","year":"2011","journal-title":"Equine Vet. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"354","DOI":"10.4314\/ovj.v10i4.1","article-title":"Radiographic parameters of the digit in a cohort population of Amiata donkeys","volume":"10","author":"Nocera","year":"2020","journal-title":"Open Vet. J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1111\/ahe.12326","article-title":"Radiography of the distal extremity of the manus in the donkey foal: Normal images and quantitative characterization from birth to 2 years of age: A pilot study","volume":"47","author":"Willekens","year":"2018","journal-title":"Anat. Histol. Embryol."},{"key":"ref_12","first-page":"31","article-title":"Radiographie des membres chez le poulain de 0 \u00e0 6 mois\u2014Images normales et pi\u00e8ges anatomiques. V\u2014Pied et paturon","volume":"26","author":"Crevier","year":"1994","journal-title":"Prat. V\u00e9t\u00e9rinaire Equine"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.livsci.2011.06.009","article-title":"Effect of horse breed and sex on growth rate and radiographic closure time of distal radial metaphyseal growth plate","volume":"141","author":"Luszczynski","year":"2011","journal-title":"Livest. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1294\/jes.31.1","article-title":"Evaluation of acquired passive immunity in mule foals up to 60 days of age","volume":"31","author":"Rusca","year":"2020","journal-title":"J. Equine Vet. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.jveb.2023.04.001","article-title":"Perinatal parameters of mule and equine foals: Similarities and differences","volume":"63","author":"Alonso","year":"2023","journal-title":"J. Vet. Behav."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.cveq.2017.03.001","article-title":"Orthopedic conditions of the premature and dysmature foal","volume":"33","author":"Coleman","year":"2017","journal-title":"Vet. Clin. N. Am. Equine Pract."},{"key":"ref_17","unstructured":"National Research Council (2007). Donkeys and Other Equids. Nutrient Requirements of Horses, The National Academy Press."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jevs.2018.02.002","article-title":"Survey radiography of the carpus and tarsus in neonatal Thoroughbred foals and appearance at 6 months of age","volume":"63","author":"Santschi","year":"2018","journal-title":"J. Equine Vet. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Rogers, C.W., Gee, E.K., and Dittmer, K.E. (2021). Growth and bone development in the horse: When is a horse skeletally mature?. Animals, 11.","DOI":"10.3390\/ani11123402"},{"key":"ref_20","unstructured":"Weaver, M., and Barakzai, S. (2009). Handbook of Equine Radiography, Elsevier Saunders."},{"key":"ref_21","unstructured":"Matthews, N.S., and Taylor, T.S. (2010). Veterinary Care of Donkeys, International Veterinary Information Service."},{"key":"ref_22","first-page":"188","article-title":"A study of correlation of closure of the distal radial physis with performance and injury in the standardbred","volume":"170","author":"Gabel","year":"1977","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_23","first-page":"15","article-title":"Radiographic assessment of skeletal maturity in the racehorse: Statistical validation and correlation with orthopaedic injuries in the Standardbred","volume":"18","author":"Pasolini","year":"2007","journal-title":"Ippologia"},{"key":"ref_24","first-page":"11","article-title":"The effect of the breeder, year and month of birth and sex on the skeletal maturation of Thoroughbred horses. Annals of Warsaw Agricultural University","volume":"29","author":"Empel","year":"1993","journal-title":"Vet. Anim. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.2460\/javma.1998.213.11.1590","article-title":"Incomplete ossification of the tarsal bones in foals: 22 cases (1988\u20131996)","volume":"213","author":"Dutton","year":"1998","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"103129","DOI":"10.1016\/j.jevs.2020.103129","article-title":"Prematurity and dysmaturity are associated with reduced height and shorter distal limb length in horses","volume":"91","author":"Clothier","year":"2020","journal-title":"J. Equine Vet. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"391","DOI":"10.2754\/avb202190040391","article-title":"Surgical management of a severe congenital deformity of the hind limb in a Shetland pony foal\u2014A case report","volume":"90","author":"Crecan","year":"2021","journal-title":"Acta Vet. Brno"},{"key":"ref_28","unstructured":"Anderson, S. (2021). Equine Reproductive Procedures, Wiley."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1111\/eve.13394","article-title":"Haematogenous septic arthritis, physitis and osteomyelitis in foals: A tutorial review on pathogenesis, diagnosis, treatment and prognosis. Part 1","volume":"33","author":"Lindegaard","year":"2021","journal-title":"Equine Vet. Educ."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1111\/eve.13393","article-title":"Haematogenous septic arthritis, physitis and osteomyelitis in foals: A tutorial review on pathogenesis, diagnosis, treatment and prognosis. Part 2","volume":"34","author":"Lindegaard","year":"2022","journal-title":"Equine Vet. 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