{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:54:50Z","timestamp":1792788890180,"version":"4.5.0"},"reference-count":77,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Alfonso X el Sabio Foundation","award":["1.010.119"],"award-info":[{"award-number":["1.010.119"]}]},{"name":"Alfonso X el Sabio Foundation","award":["FIS AESI PI21CIII\/00031"],"award-info":[{"award-number":["FIS AESI PI21CIII\/00031"]}]},{"DOI":"10.13039\/501100004587","name":"Health Research Fund, Instituto de Salud Carlos III, Ministry of Science and Innovation","doi-asserted-by":"publisher","award":["1.010.119"],"award-info":[{"award-number":["1.010.119"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Health Research Fund, Instituto de Salud Carlos III, Ministry of Science and Innovation","doi-asserted-by":"publisher","award":["FIS AESI PI21CIII\/00031"],"award-info":[{"award-number":["FIS AESI PI21CIII\/00031"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The wildcat (Felis silvestris) and the Iberian lynx (Lynx pardinus) are important species in Spain, considered as near-threatened and endangered, respectively. Both can be infected by Toxoplasma gondii, a parasite that can cause morbidity and mortality in transplacentally-infected or immunocompromised mammals. The data on the prevalence of this parasite in wild populations of these species in Spain are outdated. The objective of this study was to update information and evaluate the role of these felines in parasite epidemiology and the potential impact of the parasite on their conservation. Blood and fecal samples were collected from captured animals, as well as the tongue, diaphragm, and spleen, from animals killed in road accidents in central Spain. An indirect fluorescent antibody test (IFAT) was used to detect parasite antibodies in serum, microscopy and molecular analysis were used to detect oocysts in feces, and molecular analysis was used to determine the existence of tissue cysts. Seroprevalence was 85% in wildcats and 45% in lynx, and parasite DNA was detected in the feces of one wildcat and in tissue samples from 10 wildcats and 11 Iberian lynxes. These results highlight the epidemiological importance and high risk of T. gondii infection in animals and humans in the studied areas. Considering feline susceptibility to infection, monitoring programs are needed to assess the health status of wild felines.<\/jats:p>","DOI":"10.3390\/ani13152488","type":"journal-article","created":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T09:14:40Z","timestamp":1690881280000},"page":"2488","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Prevalence of Toxoplasma gondii in Endangered Wild Felines (Felis silvestris and Lynx pardinus) in Spain"],"prefix":"10.3390","volume":"13","author":[{"given":"Pablo","family":"Matas M\u00e9ndez","sequence":"first","affiliation":[{"name":"Facultad de Veterinaria, Universidad Alfonso X el Sabio, Villanueva de la Ca\u00f1ada, 28691 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Fuentes Corripio","sequence":"additional","affiliation":[{"name":"Laboratorio de Referencia e Investigaci\u00f3n en Parasitolog\u00eda, Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1911-8642","authenticated-orcid":false,"given":"Ana","family":"Montoya Matute","sequence":"additional","affiliation":[{"name":"Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bego\u00f1a","family":"Bailo Barroso","sequence":"additional","affiliation":[{"name":"Laboratorio de Referencia e Investigaci\u00f3n en Parasitolog\u00eda, Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3653-4528","authenticated-orcid":false,"given":"Rebeca","family":"Grande G\u00f3mez","sequence":"additional","affiliation":[{"name":"Technical Assistence, General Direction of Natural Environment and Biodiversity, Ministry of Sustainable Development, Autonomous Community of Castilla-La Mancha, 45007 Toledo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariadna","family":"Apruzzese Rubio","sequence":"additional","affiliation":[{"name":"Technical Assistence, General Direction of Natural Environment and Biodiversity, Ministry of Sustainable Development, Autonomous Community of Castilla-La Mancha, 45007 Toledo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9034-8606","authenticated-orcid":false,"given":"Francisco","family":"Ponce Gordo","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0573-4073","authenticated-orcid":false,"given":"Marta","family":"Mateo Barrientos","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Dubey, J.P. 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