{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:35:37Z","timestamp":1791894937518,"version":"4.3.4"},"reference-count":132,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T00:00:00Z","timestamp":1692748800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Toxoplasmosis is a parasitic zoonosis of veterinary importance, with implications for public health. Toxoplasma gondii infection causes abortion or congenital disease in small ruminants. Moreover, the consumption of infected meat, cured meat products, or unpasteurized milk and dairy products can facilitate zoonotic transmission. Serological studies conducted in various European countries have shown the high seroprevalence of specific anti-T. gondii antibodies in sheep and goats related to the presence of oocysts in the environment, as well as climatic conditions. This article presents the current status of the detection possibilities for T. gondii infection in small ruminants and their milk. Serological testing is considered the most practical method for diagnosing toxoplasmosis; therefore, many studies have shown that recombinant antigens as single proteins, mixtures of various antigens, or chimeric proteins can be successfully used as an alternative to Toxoplasma lysate antigens (TLA). Several assays based on DNA amplification have been developed as alternative diagnostic methods, which are especially useful when serodiagnosis is not possible, e.g., the detection of intrauterine T. gondii infection when the fetus is not immunocompetent. These techniques employ multicopy sequences highly conserved among different strains of T. gondii in conventional, nested, competitive, and quantitative reverse transcriptase-PCR.<\/jats:p>","DOI":"10.3390\/ani13172696","type":"journal-article","created":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T10:09:53Z","timestamp":1692871793000},"page":"2696","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Detection of Toxoplasma gondii Infection in Small Ruminants: Old Problems, and Current Solutions"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5421-6905","authenticated-orcid":false,"given":"Lucyna","family":"Holec-G\u0105sior","sequence":"first","affiliation":[{"name":"Department of Molecular Biotechnology and Microbiology, Faculty of Chemistry, Gda\u0144sk University of Technology, 11\/12 Narutowicza Str., 80-233 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"So\u0142owi\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnology and Microbiology, Faculty of Chemistry, Gda\u0144sk University of Technology, 11\/12 Narutowicza Str., 80-233 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,23]]},"reference":[{"key":"ref_1","unstructured":"Dubey, J.P. (2010). Toxoplasmosis of Animals and Humans, CRC Press."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"583","DOI":"10.2903\/j.efsa.2007.583","article-title":"Surveillance and Monitoring of Toxoplasma in Humans, Food and Animals\u2014Scientific Opinion of the Panel on Biological Hazards","volume":"5","author":"Andreoletti","year":"2007","journal-title":"EFSA J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/j.ijpara.2009.02.004","article-title":"Toxoplasmosis: A History of Clinical Observations","volume":"39","author":"Weiss","year":"2009","journal-title":"Int. J. Parasitol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1128\/CMR.05013-11","article-title":"Epidemiology of and Diagnostic Strategies for Toxoplasmosis","volume":"25","year":"2012","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/S0020-7519(00)00124-7","article-title":"Toxoplasma Gondii: From Animals to Humans","volume":"30","author":"Tenter","year":"2000","journal-title":"Int. J. Parasitol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.1016\/S0140-6736(04)16412-X","article-title":"Toxoplasmosis","volume":"363","author":"Montoya","year":"2004","journal-title":"Lancet"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/s13071-023-05670-y","article-title":"Zoonotic Parasites Associated with Predation by Dogs and Cats","volume":"16","author":"Otranto","year":"2023","journal-title":"Parasites Vectors"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"576","DOI":"10.26444\/aaem\/94675","article-title":"Prevalence of Toxoplasma Gondii Infection in Cats in Southwestern Poland","volume":"25","author":"Sroka","year":"2018","journal-title":"Ann. Agric. Environ. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1292\/jvms1939.52.293","article-title":"Dung Beetles, Onthophagus Spp., as Potential Transport Hosts of Feline Coccidia","volume":"52","author":"Saitoh","year":"1990","journal-title":"Japanese J. Vet. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1645\/GE-3503RN.1","article-title":"Toxoplasma Gondii Does Not Persist In Goldfish (Carassius Auratus)","volume":"91","author":"Omata","year":"2005","journal-title":"J. Parasitol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.ijppaw.2020.04.008","article-title":"Serological Evidence of Toxoplasma Gondii Infection in Melanosuchus Niger (Spix, 1825) and Caimam Crocodilus (Linnaeus, 1758)","volume":"12","author":"Ferreira","year":"2020","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S0001-706X(01)00201-7","article-title":"Host Cell Invasion by the Opportunistic Pathogen Toxoplasma Gondii","volume":"81","author":"Carruthers","year":"2002","journal-title":"Acta Trop."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/s13071-018-2632-z","article-title":"Detection and Dissemination of Toxoplasma Gondii in Experimentally Infected Calves, a Single Test Does Not Tell the Whole Story","volume":"11","author":"Burrells","year":"2018","journal-title":"Parasites and Vectors"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1590\/1678-4162-9177","article-title":"Toxoplasma Gondii in Milk of Naturally Infected Dairy Ewes on West Mesoregion of Santa Catarina State, Brazil","volume":"69","author":"Ossani","year":"2017","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1080\/00365549950163626","article-title":"Risk Factors for Toxoplasma Infection in Pregnancy: A Case-Control Study in France","volume":"31","author":"Baril","year":"1999","journal-title":"Scand. J. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1136\/bmj.321.7254.142","article-title":"Sources of Toxoplasma Infection in Pregnant Women: European Multicentre Case-Control Study","volume":"321","author":"Cook","year":"2000","journal-title":"BMJ"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1093\/oxfordjournals.aje.a008942","article-title":"Risk Factors for Toxoplasma Gondii Infection in PregnancyResults of a Prospective Case-Control Study in Norway","volume":"144","author":"Kapperud","year":"1996","journal-title":"Am. J. Epidemiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1016\/j.ijpara.2008.06.002","article-title":"Control of the Risk of Human Toxoplasmosis Transmitted by Meat","volume":"38","author":"Kijlstra","year":"2008","journal-title":"Int. J. Parasitol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.4315\/JFP-20-024","article-title":"Distribution of Toxoplasma Gondii Tissue Cysts in Shoulder Muscles of Naturally Infected Goats and Lambs","volume":"83","author":"Rani","year":"2020","journal-title":"J. Food Prot."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.rvsc.2020.06.014","article-title":"Public Health and Economic Importance of Toxoplasma Gondii Infections in Goats: The Last Decade","volume":"132","author":"Dubey","year":"2020","journal-title":"Res. Vet. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"109195","DOI":"10.1016\/j.vetpar.2020.109195","article-title":"Economic and Public Health Importance of Toxoplasma Gondii Infections in Sheep: 2009\u20132020","volume":"286","author":"Dubey","year":"2020","journal-title":"Vet. Parasitol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.vetpar.2008.12.022","article-title":"Toxoplasma Gondii and Neospora Caninum Antibodies in Sheep in the Czech Republic","volume":"161","year":"2009","journal-title":"Vet. Parasitol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/j.vetpar.2006.07.005","article-title":"Toxoplasma Gondii Infection in Sheep from Haute-Vienne, France: Seroprevalence and Isolate Genotyping by Microsatellite Analysis","volume":"142","author":"Ajzenberg","year":"2006","journal-title":"Vet. Parasitol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"604","DOI":"10.5604\/12321966.1185761","article-title":"First Report of Seroprevalence of Toxoplasma Gondii Infection in Sheep in Pomerania, Northern Poland","volume":"22","author":"Kur","year":"2015","journal-title":"Annals Agric. Environ. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"62","DOI":"10.5604\/12321966.1141370","article-title":"Toxoplasma Gondii in Small Ruminants in Northern Italy\u2014Prevalence and Risk Factors","volume":"22","author":"Gazzonis","year":"2015","journal-title":"Annals Agric. Environ. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"105137","DOI":"10.1016\/j.prevetmed.2020.105137","article-title":"Epidemiological Surveillance of Toxoplasma Gondii in Small Ruminants in Southern Spain","volume":"183","author":"Dubey","year":"2020","journal-title":"Prev. Vet. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.vetpar.2007.07.020","article-title":"Toxoplasma Gondii in Sheep from the Campania Region (Italy)","volume":"149","author":"Fusco","year":"2007","journal-title":"Vet. Parasitol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.vetpar.2010.04.008","article-title":"Toxoplasma Gondii in Wild Cervids and Sheep in Finland: North-South Gradient in Seroprevalence","volume":"171","author":"Jokelainen","year":"2010","journal-title":"Vet. Parasitol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.vetpar.2005.08.010","article-title":"Cross-Sectional Survey on Toxoplasma Gondii Infection in Cattle, Sheep and Pigs in Serbia: Seroprevalence and Risk Factors","volume":"135","author":"Klun","year":"2006","journal-title":"Vet. Parasitol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.prevetmed.2010.06.009","article-title":"Evaluation of ELISA Test Characteristics and Estimation of Toxoplasma Gondii Seroprevalence in Dutch Sheep Using Mixture Models","volume":"96","author":"Opsteegh","year":"2010","journal-title":"Prev. Vet. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.vetpar.2007.02.019","article-title":"Toxoplasma Gondii Infections in Sheep in Sicily, Southern Italy","volume":"146","author":"Vesco","year":"2007","journal-title":"Vet. Parasitol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"529","DOI":"10.2478\/v10213-012-0093-2","article-title":"Analysis of Prevalence of Toxoplasma Gondii Infection in Selected Rural Households In the Lublin Region","volume":"56","author":"Sroka","year":"2012","journal-title":"Bull. Vet. Inst. Pulawy"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.vetpar.2012.07.020","article-title":"Toxoplasma Gondii in Sheep and Goats: Seroprevalence and Potential Risk Factors under Dairy Husbandry Practices","volume":"190","author":"Tzanidakis","year":"2012","journal-title":"Vet. Parasitol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.vetpar.2013.09.017","article-title":"Toxoplasma Gondii and Neospora Caninum Seroprevalence in Dairy Sheep and Goats Mixed Stock Farming","volume":"198","author":"Anastasia","year":"2013","journal-title":"Vet. Parasitol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1925","DOI":"10.1007\/s00436-017-5470-6","article-title":"Seroprevalence of Neospora Caninum and Toxoplasma Gondii in Exotic Ruminants and Camelids in the Czech Republic","volume":"116","author":"Lamka","year":"2017","journal-title":"Parasitol. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1111\/j.1751-0813.1954.tb08204.x","article-title":"New Zealand Type II Abortion in Ewes","volume":"30","author":"Hartley","year":"1954","journal-title":"Aust. Vet. J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1080\/00480169.1957.33275","article-title":"Toxoplasmosis as a Cause of Ovine Perinatal Mortality","volume":"5","author":"Hartley","year":"1957","journal-title":"N. Z. Vet. J."},{"key":"ref_38","unstructured":"Dubey, J.P., and Beattie, C.P. (1988). Parasitology, CRC Press."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1887","DOI":"10.1017\/S0031182009991636","article-title":"Ovine Toxoplasmosis","volume":"136","author":"Innes","year":"2009","journal-title":"Parasitology"},{"key":"ref_40","first-page":"100456","article-title":"Seroepidemiology of Toxoplasma Gondii in Small Ruminants in Northwest Ethiopia","volume":"22","author":"Esubalew","year":"2020","journal-title":"Vet. Parasitol. Reg. Stud. Reports"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1007\/s11250-023-03603-6","article-title":"Toxoplasma Gondii Infection in Egyptian Domestic Sheep and Goats: Seroprevalence and Risk Factors","volume":"55","author":"Selim","year":"2023","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/S0167-5877(98)00057-9","article-title":"Risk Factors for the Presence of Antibodies to Toxoplasma Gondii in Norwegian Slaughter Lambs","volume":"35","author":"Skjerve","year":"1998","journal-title":"Prev. Vet. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1016\/j.rvsc.2013.08.007","article-title":"Some Risk Factors for Reproductive Failures and Contribution of Toxoplasma Gondii Infection in Sheep and Goats of Central Ethiopia: A Cross-Sectional Study","volume":"95","author":"Gebremedhin","year":"2013","journal-title":"Res. Vet. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.vetpar.2007.07.003","article-title":"Toxoplasma Gondii and Ovine Toxoplasmosis: New Aspects of an Old Story","volume":"149","author":"Buxton","year":"2007","journal-title":"Vet. Parasitol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1016\/S0020-7519(01)00282-X","article-title":"High Levels of Congenital Transmission of Toxoplasma Gondii in a Commercial Sheep Flock","volume":"31","author":"Duncanson","year":"2001","journal-title":"Int. J. Parasitol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1017\/S0031182004006614","article-title":"High Levels of Congenital Transmission of Toxoplasma Gondii in Longitudinal and Cross-Sectional Studies on Sheep Farms Provides Evidence of Vertical Transmission in Ovine Hosts","volume":"130","author":"Williams","year":"2005","journal-title":"Parasitology"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1017\/S0031182007003721","article-title":"Evidence That Primary Infection of Charollais Sheep with Toxoplasma Gondii May Not Prevent Foetal Infection and Abortion in Subsequent Lambings","volume":"135","author":"Morley","year":"2008","journal-title":"Parasitology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0304-4017(01)00526-X","article-title":"Single Tube Nested PCR for the Detection of Toxoplasma Gondii in Fetal Tissues from Naturally Aborted Ewes","volume":"102","author":"Hurtado","year":"2001","journal-title":"Vet. Parasitol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1128\/jcm.27.8.1787-1792.1989","article-title":"Direct and Sensitive Detection of a Pathogenic Protozoan, Toxoplasma Gondii, by Polymerase Chain Reaction","volume":"27","author":"Burg","year":"1989","journal-title":"J. Clin. Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0020-7519(99)00170-8","article-title":"Identification of a 200- to 300-Fold Repetitive 529 Bp DNA Fragment in Toxoplasma Gondii, and Its Use for Diagnostic and Quantitative PCR","volume":"30","author":"Homan","year":"2000","journal-title":"Int. J. Parasitol."},{"key":"ref_51","first-page":"173","article-title":"Species-Specific Identification of Sarcocystis and Toxoplasma by PCR Amplification of Small Subunit Ribosomal RNA Gene Fragments","volume":"35","author":"Tenter","year":"1994","journal-title":"Appl. Parasitol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1099\/00222615-32-1-25","article-title":"Polymerase Chain Reaction for Detection of Toxoplasma Gondii","volume":"32","author":"Savva","year":"1990","journal-title":"J. Med. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.actatropica.2016.03.035","article-title":"An Alternative Nested-PCR Assay for the Detection of Toxoplasma Gondii Strains Based on GRA7 Gene Sequences","volume":"159","author":"Costa","year":"2016","journal-title":"Acta Trop."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1136\/jcp.52.1.61","article-title":"Quantitation of Toxoplasma Gondii DNA in a Competitive Nested Polymerase Chain Reaction","volume":"52","author":"Lee","year":"1999","journal-title":"J. Clin. Pathol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"4121","DOI":"10.1128\/JCM.38.11.4121-4125.2000","article-title":"Real-Time PCR for Quantitative Detection of Toxoplasma Gondii","volume":"38","author":"Lin","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.foodcont.2015.06.007","article-title":"Development of a QRT-PCR Method to Assess the Viability of Giardia Intestinalis Cysts, Cryptosporidium Spp. and Toxoplasma Gondii Oocysts","volume":"59","author":"Gargala","year":"2016","journal-title":"Food Control"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Ranucci, D., Battisti, E., Veronesi, F., Diaferia, M., Morganti, G., Branciari, R., Ferroglio, E., Valiani, A., and Chiesa, F. (2020). Absence of Viable Toxoplasma Gondii in Artisanal Raw-Milk Ewe Cheese Derived from Naturally Infected Animals. Microorganisms, 8.","DOI":"10.3390\/microorganisms8010143"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.mimet.2010.03.023","article-title":"Using Quantitative Reverse Transcriptase PCR and Cell Culture Plaque Assays to Determine Resistance of Toxoplasma Gondii Oocysts to Chemical Sanitizers","volume":"81","author":"Villegas","year":"2010","journal-title":"J. Microbiol. Methods"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.vetpar.2017.05.009","article-title":"Molecular Detection of Toxoplasma Gondii in the Slaughter Sheep and Goats from North India","volume":"241","author":"Kalambhe","year":"2017","journal-title":"Vet. Parasitol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.meatsci.2017.07.004","article-title":"Molecular Detection of Toxoplasma Gondii Infection in Slaughtered Ruminants (Sheep, Goats and Cattle) in Northwest Tunisia","volume":"133","author":"Amdouni","year":"2017","journal-title":"Meat Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1002\/vms3.53","article-title":"Molecular Detection and Phylogenetic Analyses of Toxoplasma Gondii from Naturally Infected Sheep in Northern and Central Tunisia","volume":"3","author":"Rouatbi","year":"2017","journal-title":"Vet. Med. Sci."},{"key":"ref_62","first-page":"37","article-title":"Prevalence of Cerebral Toxoplasmosis among Slaughtered Sheep in Semnan, Iran","volume":"64","author":"Gorji","year":"2018","journal-title":"Ann. Parasitol."},{"key":"ref_63","first-page":"100341","article-title":"Detection of Toxoplasma Gondii Infection in Semen of Rams Used for Natural Mating in Commercial Sheep Farms in Tunisia","volume":"18","author":"Rouatbi","year":"2019","journal-title":"Vet. Parasitol. Reg. Stud. Reports"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Hamid, N.A., Sadiq, M.B., Ramanoon, S.Z., Mansor, R., Watanabe, M., Mahiza, N., Isa, M., Kamaludeen, J., and Syed-Hussain, S.S. (2020). Seroprevalence and Risk Factors of Toxoplasma Gondii in Ruminant Meats from Wet Markets in Klang Valley and Abattoirs in Selangor, Malaysia. Animals, 10.","DOI":"10.3390\/ani10071139"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"102060","DOI":"10.1016\/j.parint.2020.102060","article-title":"Toxoplasma Gondii Infection in Meat-Producing Small Ruminants: Meat Juice Serology and Genotyping","volume":"76","author":"Gazzonis","year":"2020","journal-title":"Parasitol. Int."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1089\/vbz.2019.2488","article-title":"Molecular Detection of Toxoplasma Gondii in the Slaughter Sheep and Goats from Shandong Province, Eastern China","volume":"20","author":"Ai","year":"2020","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"101530","DOI":"10.1016\/j.cimid.2020.101530","article-title":"Molecular Detection of the B1 Gene of Toxoplasma Gondii in Blood Samples of Female Sheep and Goats in Tebessa, Northeastern Algeria","volume":"72","author":"Abdelouahed","year":"2020","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/1753-6405.12955","article-title":"Lamb as a Potential Source of Toxoplasma Gondii Infection for Australians","volume":"44","author":"Dawson","year":"2020","journal-title":"Aust. N. Z. J. Public Health"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/s00003-020-01306-w","article-title":"Molecular and Serological Detection and of Toxoplasma Gondii in Small Ruminants of Southwest Iran and the Potential Risks for Consumers","volume":"16","author":"Yousefvand","year":"2021","journal-title":"J. Consum. Prot. Food Saf."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"109593","DOI":"10.1016\/j.vetpar.2021.109593","article-title":"High Toxoplasma Gondii Frequency of Infection in the Genital Tract of Ewes Previously Positive for the Parasite DNA in Meat, in Sidi Bouzid, Tunisia","volume":"300","author":"Rouatbi","year":"2021","journal-title":"Vet. Parasitol."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Bahreh, M., Hajimohammadi, B., and Eslami, G. (2021). Toxoplasma Gondii in Sheep and Goats from Central Iran. BMC Res. Notes, 14.","DOI":"10.1186\/s13104-021-05465-3"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"106304","DOI":"10.1016\/j.actatropica.2022.106304","article-title":"First Report on Molecular Epidemiology, Seasonality and Phylogeny of Toxoplasma Gondii Infecting Goats from Khanewal District in Punjab, Pakistan","volume":"228","author":"Aziz","year":"2022","journal-title":"Acta Trop."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"106139","DOI":"10.1016\/j.actatropica.2021.106139","article-title":"Seroprevalence and Molecular Characterization of Toxoplasma Gondii Infecting Ruminants in the North-West of Egypt","volume":"225","author":"Khattab","year":"2022","journal-title":"Acta Trop."},{"key":"ref_74","first-page":"100688","article-title":"Toxoplasma Gondii in Tissues of Sheep Slaughtered for Human Consumption in a Highly Endemic Area for Toxoplasmosis in Brazil","volume":"29","author":"Silva","year":"2022","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s13071-022-05634-8","article-title":"Toxoplasma Gondii Infection in Sheep from Romania","volume":"16","author":"Mircean","year":"2023","journal-title":"Parasit. Vectors"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"101928","DOI":"10.1016\/j.cimid.2022.101928","article-title":"High Infection Rates of Toxoplasma Gondii in Cattle, Sheep and Pigs from Israel","volume":"92","author":"Mazuz","year":"2023","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Ji, M.-J., Cho, H.-C., Park, Y.-J., Jang, D.-H., Park, J., and Choi, K.-S. (2023). Molecular Detection of Toxoplasma Gondii in Blood Samples of Domestic Livestock in the Republic of Korea. Pathogens, 12.","DOI":"10.3390\/pathogens12040547"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/S0022-3476(75)80825-0","article-title":"Toxoplasmosis in an Infant Fed Unpasteurized Goat Milk","volume":"87","author":"Riemann","year":"1975","journal-title":"J. Pediatr."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1728","DOI":"10.1001\/jama.1982.03330140038029","article-title":"Toxoplasmosis Infection Associated with Raw Goat\u2019s Milk","volume":"248","author":"Sacks","year":"1982","journal-title":"JAMA"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"359","DOI":"10.3109\/00365549009027060","article-title":"Simultaneous Diagnosis of Toxoplasmosis in Goats and Goatowner\u2019s Family","volume":"22","author":"Skinner","year":"1990","journal-title":"Scand. J. Infect. Dis."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1086\/605433","article-title":"Risk Factors for Toxoplasma Gondii Infection in the United States","volume":"49","author":"Jones","year":"2009","journal-title":"Clin. Infect. Dis."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1093\/eurpub\/cks075","article-title":"Seroprevalence and Risk Factors for Toxoplasmosis among Antenatal Women in London: A Re-Examination of Risk in an Ethnically Diverse Population","volume":"23","author":"Flatt","year":"2013","journal-title":"Eur. J. Public Health"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"74","DOI":"10.2307\/3282630","article-title":"Prevalence of Toxoplasma Gondii Antibodies in Nine Populations of Dairy Goats: Compared Titers Using Modified Direct Agglutination and Indirect Hemagglutination","volume":"76","author":"Patton","year":"1990","journal-title":"J. Parasitol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.actatropica.2014.11.011","article-title":"Occurrence of Anti-Toxoplasma Gondii Antibodies and Parasite DNA in Raw Milk of Sheep and Goats of Local Breeds Reared in Northeastern Brazil","volume":"142","author":"Alves","year":"2015","journal-title":"Acta Trop."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"023","DOI":"10.14411\/fp.2017.023","article-title":"Seroprevalence of Toxoplasma Gondii Infection in Goats from the South-West Region of Poland and the Detection of T. Gondii DNA in Goat Milk","volume":"64","author":"Sroka","year":"2017","journal-title":"Folia Parasitol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1002\/vms3.29","article-title":"Molecular Prevalence of Toxoplasma Gondii DNA in Goats\u2019 Milk and Seroprevalence in Northwest Tunisia","volume":"2","author":"Amairia","year":"2016","journal-title":"Vet. Med. Sci."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.4315\/0362-028X.JFP-14-167","article-title":"Detection and Survival of Toxoplasma Gondii in Milk and Cheese from Experimentally Infected Goats 3","volume":"77","author":"Dubey","year":"2014","journal-title":"J. Food Prot."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"905326","DOI":"10.1155\/2013\/905326","article-title":"Seroprevalence, Detection of DNA in Blood and Milk, and Genotyping of Toxoplasma Gondii in a Goat Population in Italy","volume":"2013","author":"Mancianti","year":"2013","journal-title":"Biomed Res. Int."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.vetpar.2014.12.002","article-title":"DNA Detection of Toxoplasma Gondii in Sheep Milk and Blood Samples in Relation to Phase of Infection","volume":"208","author":"Luptakova","year":"2014","journal-title":"Vet. Parasitol."},{"key":"ref_90","first-page":"45","article-title":"Toxoplasma Gondii and Pre-Treatment Protocols for Polymerase Chain Reaction Analysis of Milk Samples: A Field Trial in Sheep from Southern Italy","volume":"6","author":"Vismarra","year":"2017","journal-title":"Ital. J. Food Saf."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.vetpar.2016.12.010","article-title":"Survival of Toxoplasma Gondii Tachyzoites in Simulated Gastric Fluid and Cow\u2019s Milk","volume":"233","author":"Koethe","year":"2017","journal-title":"Vet. Parasitol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"202","DOI":"10.3923\/ajas.2011.202.207","article-title":"The Viability and Infectivity of Toxoplasma Gondii Tachyzoites in Dairy Products Undergoing Food Processing","volume":"5","author":"Asgari","year":"2011","journal-title":"Asian J. Anim. Sci."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.vetpar.2010.12.007","article-title":"Detection of Toxoplasma Gondii DNA in the Milk of Naturally Infected Ewes","volume":"177","author":"Camossi","year":"2011","journal-title":"Vet. Parasitol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1089\/fpd.2016.2203","article-title":"Presence of Pathogenic Rickettsiae and Protozoan in Samples of Raw Milk from Cows, Goats, and Sheep","volume":"14","author":"Cisak","year":"2017","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.1007\/s00436-019-06306-w","article-title":"Detection and Genotypic Characterization of Toxoplasma Gondii DNA within the Milk of Mongolian Livestock","volume":"118","author":"Iacobucci","year":"2019","journal-title":"Parasitol. Res."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.rvsc.2022.07.020","article-title":"Molecular Detection of Toxoplasma Gondii DNA in Goats (Capra Hircus), Sheep (Ovis Aries), and Donkey (Equus Asinus) Milk Using PCR in East Azerbaijan Province, Iran","volume":"152","author":"Deljavan","year":"2022","journal-title":"Res. Vet. Sci."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1111\/tbed.12160","article-title":"Detection of Toxoplasma Gondii in the Milk of Naturally Infected Goats in the Northeast of Brazil","volume":"62","author":"Bezerra","year":"2015","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"104738","DOI":"10.1016\/j.prevetmed.2019.104738","article-title":"Toxoplasma Gondii in Naturally Infected Goats: Monitoring of Specific IgG Levels in Serum and Milk during Lactation and Parasitic DNA Detection in Milk","volume":"170","author":"Gazzonis","year":"2019","journal-title":"Prev. Vet. Med."},{"key":"ref_99","first-page":"601","article-title":"Toxoplasma Gondii in Milk of Human and Goat from the Desert Area in Central Iran","volume":"16","author":"Khamsian","year":"2021","journal-title":"Iran. J. Parasitol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"2133","DOI":"10.1128\/jcm.27.9.2133-2135.1989","article-title":"Significance of Specific Immunoglobulin M in the Chronological Diagnosis of 38 Cases of Toxoplasmic Lymphadenopathy","volume":"27","author":"Canessa","year":"1989","journal-title":"J. Clin. Microbiol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1067\/mob.2001.108341","article-title":"Confirmatory Serologic Testing for Acute Toxoplasmosis and Rate of Induced Abortions among Women Reported to Have Positive Toxoplasma Immunoglobulin M Antibody Titers","volume":"184","author":"Liesenfeld","year":"2001","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.1128\/JCM.00169-13","article-title":"Toxoplasma Seroconversion with Negative or Transient Immunoglobulin M in Pregnant Women: Myth or Reality? A French Multicenter Retrospective Study","volume":"51","author":"Cimon","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"3601","DOI":"10.1128\/JCM.01663-15","article-title":"Significance of a Positive Toxoplasma Immunoglobulin M Test Result in the United States","volume":"53","author":"Dhakal","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"ref_104","first-page":"529812","article-title":"Review on the Current Trends of Toxoplasmosis Serodiagnosis in Humans","volume":"10","author":"Nishikawa","year":"2020","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_105","doi-asserted-by":"crossref","unstructured":"Holec-G\u0105sior, L., and So\u0142owi\u0144ska, K. (2022). IgG Avidity Test as a Tool for Discrimination between Recent and Distant Toxoplasma Gondii Infection\u2014Current Status of Studies. Antibodies, 11.","DOI":"10.3390\/antib11030052"},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s00436-003-0964-9","article-title":"Immunodiagnosis of Primary Toxoplasma Gondii Infection in Sheep by the Use of a P30 IgG Avidity ELISA","volume":"91","author":"Sager","year":"2003","journal-title":"Parasitol. Res."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.vetpar.2007.02.036","article-title":"Seroprevalence and Toxoplasma Gondii-IgG Avidity in Sheep from Lajes, Brazil","volume":"146","author":"Clementino","year":"2007","journal-title":"Vet. Parasitol."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1128\/CVI.00117-13","article-title":"Toxoplasma Gondii Recombinant Antigens as Tools for Serodiagnosis of Human Toxoplasmosis: Current Status of Studies","volume":"20","year":"2013","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_109","doi-asserted-by":"crossref","unstructured":"Ferra, B., Holec-Gasior, L., and Gra\u017alewska, W. (2020). Toxoplasma Gondii Recombinant Antigens in the Serodiagnosis of Toxoplasmosis in Domestic and Farm Animals. Animals, 10.","DOI":"10.3390\/ani10081245"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/0304-4017(92)90160-B","article-title":"Development of ELISAs Based on Recombinant Antigens for the Detection of Toxoplasma Gondii-Specific Antibodies in Sheep and Cats","volume":"43","author":"Tenter","year":"1992","journal-title":"Vet. Parasitol."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.vetpar.2014.09.018","article-title":"The Optimal Mixture of Toxoplasma Gondii Recombinant Antigens (GRA1, P22, ROP1) for Diagnosis of Ovine Toxoplasmosis","volume":"206","author":"Ferra","year":"2014","journal-title":"Vet. Parasitol."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.parint.2015.03.004","article-title":"Serodiagnosis of Toxoplasma Gondii Infection in Farm Animals (Horses, Swine, and Sheep) by Enzyme-Linked Immunosorbent Assay Using Chimeric Antigens","volume":"64","author":"Ferra","year":"2015","journal-title":"Parasitol. Int."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"3291","DOI":"10.1007\/s00436-015-4552-6","article-title":"A New Toxoplasma Gondii Chimeric Antigen Containing Fragments of SAG2, GRA1, and ROP1 Proteins\u2014Impact of Immunodominant Sequences Size on Its Diagnostic Usefulness","volume":"114","author":"Ferra","year":"2015","journal-title":"Parasitol. Res."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1128\/CVI.00452-12","article-title":"MIC1-MAG1-SAG1 Chimeric Protein, a Most Effective Antigen for Detection of Human Toxoplasmosis","volume":"19","author":"Ferra","year":"2012","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1128\/CVI.05433-11","article-title":"A New MIC1-MAG1 Recombinant Chimeric Antigen Can Be Used Instead of the Toxoplasma Gondii Lysate Antigen in Serodiagnosis of Human Toxoplasmosis","volume":"19","author":"Ferra","year":"2012","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.diagmicrobio.2015.01.018","article-title":"New Recombinant Chimeric Antigens, P35-MAG1, MIC1-ROP1, and MAG1-ROP1, for the Serodiagnosis of Human Toxoplasmosis","volume":"82","author":"Kur","year":"2015","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_117","doi-asserted-by":"crossref","unstructured":"Ferra, B., Holec-G\u0105sior, L., Gatkowska, J., Dziadek, B., Dzitko, K., Gr\u0105\u017alewska, W., and Lautenbach, D. (2019). The First Study on the Usefulness of Recombinant Tetravalent Chimeric Proteins Containing Fragments of SAG2, GRA1, ROP1 and AMA1 Antigens in the Detection of Specific Anti-Toxoplasma Gondii Antibodies in Mouse and Human Sera. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0217866"},{"key":"ref_118","doi-asserted-by":"crossref","unstructured":"Holec-G\u0105sior, L., Ferra, B., and Gr\u0105\u017alewska, W. (2019). Toxoplasma Gondii Tetravalent Chimeric Proteins as Novel Antigens for Detection of Specific Immunoglobulin G in Sera of Small Ruminants. Animals, 9.","DOI":"10.3390\/ani9121146"},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.vetpar.2008.03.032","article-title":"High-Level Expression of SAG1 and GRA7 Gene of Toxoplasma Gondii (Izatnagar Isolate) and Their Application in Serodiagnosis of Goat Toxoplasmosis","volume":"154","author":"Velmurugan","year":"2008","journal-title":"Vet. Parasitol."},{"key":"ref_120","first-page":"111","article-title":"Serodiagnosis of Ovine Toxoplasmosis in Mongolia by an Enzyme-Linked Immunosorbent Assay with Recombinant Toxoplasma Gondii Matrix Antigen 1","volume":"58","author":"Tumurjav","year":"2010","journal-title":"Jpn. J. Vet. Res."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s11250-014-0703-5","article-title":"Detection of Antibodies to Toxoplasma Gondii in Domesticated Ruminants by Recombinant Truncated SAG2 Enzyme-Linked Immunosorbent Assay","volume":"47","author":"Singh","year":"2015","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"1877","DOI":"10.1292\/jvms.16-0234","article-title":"Enzyme-Linked Immunosorbent Assays Using Recombinant TgSAG2 and NcSAG1 to detect Toxoplasma Gondii and Neospora Caninum-Specific in Domestic Animals in Turkey","volume":"78","author":"Zhou","year":"2016","journal-title":"J. Vet. Med. Sci."},{"key":"ref_123","first-page":"1","article-title":"Seroprevalence and Epidemiology of Toxoplasma Gondii in Farm Animals in Different Regions of Egypt","volume":"3\u20134","author":"Fereig","year":"2016","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_124","first-page":"61","article-title":"High Seroprevalence of Toxoplasma Gondii in Goats in Jharkhand State of India","volume":"12","author":"Bachan","year":"2018","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_125","doi-asserted-by":"crossref","unstructured":"Jirapattharasate, C., Udonsom, R., Prachasuphap, A., Jongpitisub, K., and Dhepakson, P. (2021). Development and Evaluation of Recombinant GRA8 Protein for the Serodiagnosis of Toxoplasma Gondii Infection in Goats. BMC Vet. Res., 17.","DOI":"10.1186\/s12917-020-02719-3"},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"1029768","DOI":"10.3389\/fcimb.2022.1029768","article-title":"Application of Toxoplasma Gondii-Specific SAG1, GRA7 and BAG1 Proteins in Serodiagnosis of Animal Toxoplasmosis","volume":"12","author":"Qi","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/0169-4758(93)90236-9","article-title":"Toxoplasmosis: The First Commercial Vaccine","volume":"9","author":"Buxton","year":"1993","journal-title":"Parasitol. Today"},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1136\/vr.133.13.310","article-title":"Experimental Challenge of Sheep 18 Months after Vaccination with a Live (S48) Toxoplasma Gondii Vaccine","volume":"133","author":"Buxton","year":"1993","journal-title":"Vet. Rec."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.pt.2008.09.008","article-title":"Toxoplasma-Safe Meat: Close to Reality?","volume":"25","author":"Kijlstra","year":"2009","journal-title":"Trends Parasitol."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"4339","DOI":"10.1021\/acs.jproteome.0c00484","article-title":"Rapid Responseto Pandemic Threats: Immunogenic Epitope of Pandemic Pathogens for Diagnostics and Vaccine Development Peptide Microarrays","volume":"19","author":"Heiss","year":"2020","journal-title":"J. Proteome Res."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.exppara.2012.09.015","article-title":"Toxoplasma Gondii: Bioinformatics Analysis, Cloning and Expression of a Novel Protein TgIMP1","volume":"132","author":"Bai","year":"2012","journal-title":"Exp. Parasitol."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"870904","DOI":"10.3389\/fvets.2022.870904","article-title":"The Global Prevalence of Neospora Caninum Infection in Sheep and Goats That Had an Abortion and Aborted Fetuses: A Systematic Review and Meta-Analysis","volume":"9","author":"Nayeri","year":"2022","journal-title":"Front. Vet. 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