{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T16:14:32Z","timestamp":1789575272861,"version":"build-2803163510"},"reference-count":56,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T00:00:00Z","timestamp":1693440000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Guangdong Basic and Applied Basic Research Foundation","award":["2022A1515110474"],"award-info":[{"award-number":["2022A1515110474"]}]},{"name":"Guangdong Basic and Applied Basic Research Foundation","award":["2021B1515120006"],"award-info":[{"award-number":["2021B1515120006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Blastocystis spp., Enterocytozoon bieneusi, and Giardia duodenalis are three common zoonotic intestinal parasites that cause severe diarrhea and enteric diseases. Leizhou black goats are characterized by a high reproductive rate, fast growth, and good meat quality, making them one of the pre-eminent goat breeds in China. Goats are reportedly common reservoirs of these three intestinal pathogens, but no information on their prevalence or genotypic distributions in black goats in Guangdong Province, China, is available. A total of 226 fecal samples were collected from goats in Zhanjiang city and genomic DNA was extracted from them. The presence of the three pathogens was detected using nested PCR targeting the sequences encoding SSU rRNA (Blastocystis spp.), the internal transcribed spacer of rRNA (ITS; E. bieneusi), as well as beta-giardin, glutamate dehydrogenase, and triosephosphate isomerase (G. duodenalis). All PCR products were sequenced to determine the species and genotypes of the organisms. The total prevalence rates of Blastocystis spp., E. bieneusi, and G. duodenalis were 33.63% (76\/226), 17.70% (40\/226), and 24.78% (56\/226), respectively. Four subtypes of Blastocystis spp. were detected: ST5 (n = 6), ST10 (n = 50), ST14 (n = 14), and ST21 (n = 6). Among them, ST10 was the dominant genotype, accounting for 65.79% of strains, followed by the genotypes ST14 (18.42%), zoonotic ST5 (7.89%), and ST21 (7.89%). Four genotypes of E. bieneusi were detected: CHG3 (n = 32), CM21 (n = 4), CHG1 (n = 2), and ET-L2 (n = 2). Among these, CHG3 was the dominant genotype. Assemblage E (n = 54) and concurrent assemblages A and E (n = 2) were identified in the G. duodenalis-positive goats using multilocus genotyping. Blastocystis spp., E. bieneusi, and G. duodenalis infections were common in Leizhou black goats, all of which have zoonotic genotypes, indicating the potential risk of zoonotic transmission. Our results provide basic data for the prevention and control of these three intestinal pathogens. Further studies are required to better understand their genetic characteristics and zoonotic potential in Guangdong Province.<\/jats:p>","DOI":"10.3390\/ani13172777","type":"journal-article","created":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T11:33:25Z","timestamp":1693481605000},"page":"2777","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Occurrence and Genotypic Identification of Blastocystis spp., Enterocytozoon bieneusi, and Giardia duodenalis in Leizhou Black Goats in Zhanjiang City, Guangdong Province, China"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2363-858X","authenticated-orcid":false,"given":"Xingang","family":"Yu","sequence":"first","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongcai","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yilong","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanru","family":"Mu","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaijian","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anfeng","family":"Wu","sequence":"additional","affiliation":[{"name":"Maccura Biotechnology Co., Ltd., Chengdu 510000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianchao","family":"Guo","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Animal Husbandry Technology Promotion Station, Guangzhou 510500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Hong","sequence":"additional","affiliation":[{"name":"National Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention (Chinese Center for Tropical Diseases Research), Key Laboratory of Parasite and Vector Biology, National Health Commission of the People\u2019s Republic of China (NHC), World Health Organization (WHO) Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoji","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Muadica, A.S., K\u00f6ster, P.C., Dashti, A., Bailo, B., Hern\u00e1ndez-de-Mingo, M., and Reh, L. 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