{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T18:41:13Z","timestamp":1788374473260,"version":"build-2803163510"},"reference-count":86,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T00:00:00Z","timestamp":1693872000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1302103"],"award-info":[{"award-number":["2022YFD1302103"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["CARS-37"],"award-info":[{"award-number":["CARS-37"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["25-LZIHPS-01"],"award-info":[{"award-number":["25-LZIHPS-01"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["21JR7RA029"],"award-info":[{"award-number":["21JR7RA029"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"China Agriculture Research System of MOF and MARA","award":["2022YFD1302103"],"award-info":[{"award-number":["2022YFD1302103"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["CARS-37"],"award-info":[{"award-number":["CARS-37"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["25-LZIHPS-01"],"award-info":[{"award-number":["25-LZIHPS-01"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["21JR7RA029"],"award-info":[{"award-number":["21JR7RA029"]}]},{"name":"Innovation Project of Chinese Academy of Agricultural Sciences","award":["2022YFD1302103"],"award-info":[{"award-number":["2022YFD1302103"]}]},{"name":"Innovation Project of Chinese Academy of Agricultural Sciences","award":["CARS-37"],"award-info":[{"award-number":["CARS-37"]}]},{"name":"Innovation Project of Chinese Academy of Agricultural Sciences","award":["25-LZIHPS-01"],"award-info":[{"award-number":["25-LZIHPS-01"]}]},{"name":"Innovation Project of Chinese Academy of Agricultural Sciences","award":["21JR7RA029"],"award-info":[{"award-number":["21JR7RA029"]}]},{"name":"Gansu Natural Science Foundation of China","award":["2022YFD1302103"],"award-info":[{"award-number":["2022YFD1302103"]}]},{"name":"Gansu Natural Science Foundation of China","award":["CARS-37"],"award-info":[{"award-number":["CARS-37"]}]},{"name":"Gansu Natural Science Foundation of China","award":["25-LZIHPS-01"],"award-info":[{"award-number":["25-LZIHPS-01"]}]},{"name":"Gansu Natural Science Foundation of China","award":["21JR7RA029"],"award-info":[{"award-number":["21JR7RA029"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Studying the mechanism of spermatogenesis is key to exploring the reproductive characteristics of male yaks. Although N6-methyladenosine (m6A) RNA modification has been reported to regulate spermatogenesis and reproductive function in mammals, the molecular mechanism of m6A in yak testis development and spermatogenesis remains largely unknown. Therefore, we collected testicular tissue from juvenile and adult yaks and found that the m6A level significantly increased after sexual maturity in yaks. In MeRIP-seq, 1702 hypermethylated peaks and 724 hypomethylated peaks were identified. The hypermethylated differentially methylated RNAs (DMRs) (CIB2, AK1, FOXJ2, PKDREJ, SLC9A3, and TOPAZ1) mainly regulated spermatogenesis. Functional enrichment analysis showed that DMRs were significantly enriched in the adherens junction, gap junction, and Wnt, PI3K, and mTOR signaling pathways, regulating cell development, spermatogenesis, and testicular endocrine function. The functional analysis of differentially expressed genes showed that they were involved in the biological processes of mitosis, meiosis, and flagellated sperm motility during the sexual maturity of yak testis. We also screened the key regulatory factors of testis development and spermatogenesis by combined analysis, which included BRCA1, CREBBP, STAT3, and SMAD4. This study indexed the m6A characteristics of yak testicles at different developmental stages, providing basic data for further research of m6A modification regulating yak testicular development.<\/jats:p>","DOI":"10.3390\/ani13182815","type":"journal-article","created":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T09:59:31Z","timestamp":1693907971000},"page":"2815","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Transcriptome Studies Reveal the N6-Methyladenosine Differences in Testis of Yaks at Juvenile and Sexual Maturity Stages"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7162-6989","authenticated-orcid":false,"given":"Shaoke","family":"Guo","sequence":"first","affiliation":[{"name":"Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, 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and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China"},{"name":"Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou 730050, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5755-6673","authenticated-orcid":false,"given":"Mengli","family":"Cao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China"},{"name":"Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou 730050, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7960-2423","authenticated-orcid":false,"given":"Lin","family":"Xiong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China"},{"name":"Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou 730050, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8844-3321","authenticated-orcid":false,"given":"Yandong","family":"Kang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China"},{"name":"Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou 730050, 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