{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:08:17Z","timestamp":1792789697947,"version":"4.5.0"},"reference-count":41,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T00:00:00Z","timestamp":1693958400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Program of China","award":["2021YFE0111100"],"award-info":[{"award-number":["2021YFE0111100"]}]},{"name":"National Key R&amp;D Program of China","award":["KY202201002"],"award-info":[{"award-number":["KY202201002"]}]},{"name":"National Key R&amp;D Program of China","award":["GJ2021015"],"award-info":[{"award-number":["GJ2021015"]}]},{"name":"National Key R&amp;D Program of China","award":["CARS-40-k01"],"award-info":[{"award-number":["CARS-40-k01"]}]},{"name":"National Key R&amp;D Program of China","award":["SJCX22_1991"],"award-info":[{"award-number":["SJCX22_1991"]}]},{"name":"Science and Technology Partnership Program","award":["2021YFE0111100"],"award-info":[{"award-number":["2021YFE0111100"]}]},{"name":"Science and Technology Partnership Program","award":["KY202201002"],"award-info":[{"award-number":["KY202201002"]}]},{"name":"Science and Technology Partnership Program","award":["GJ2021015"],"award-info":[{"award-number":["GJ2021015"]}]},{"name":"Science and Technology Partnership Program","award":["CARS-40-k01"],"award-info":[{"award-number":["CARS-40-k01"]}]},{"name":"Science and Technology Partnership Program","award":["SJCX22_1991"],"award-info":[{"award-number":["SJCX22_1991"]}]},{"name":"Ministry of Science and Technology of China","award":["2021YFE0111100"],"award-info":[{"award-number":["2021YFE0111100"]}]},{"name":"Ministry of Science and Technology of China","award":["KY202201002"],"award-info":[{"award-number":["KY202201002"]}]},{"name":"Ministry of Science and Technology of China","award":["GJ2021015"],"award-info":[{"award-number":["GJ2021015"]}]},{"name":"Ministry of Science and Technology of China","award":["CARS-40-k01"],"award-info":[{"award-number":["CARS-40-k01"]}]},{"name":"Ministry of Science and Technology of China","award":["SJCX22_1991"],"award-info":[{"award-number":["SJCX22_1991"]}]},{"name":"earmarked fund for CARS-18","award":["2021YFE0111100"],"award-info":[{"award-number":["2021YFE0111100"]}]},{"name":"earmarked fund for CARS-18","award":["KY202201002"],"award-info":[{"award-number":["KY202201002"]}]},{"name":"earmarked fund for CARS-18","award":["GJ2021015"],"award-info":[{"award-number":["GJ2021015"]}]},{"name":"earmarked fund for CARS-18","award":["CARS-40-k01"],"award-info":[{"award-number":["CARS-40-k01"]}]},{"name":"earmarked fund for CARS-18","award":["SJCX22_1991"],"award-info":[{"award-number":["SJCX22_1991"]}]},{"name":"Zhenjiang Science and Technology support project","award":["2021YFE0111100"],"award-info":[{"award-number":["2021YFE0111100"]}]},{"name":"Zhenjiang Science and Technology support project","award":["KY202201002"],"award-info":[{"award-number":["KY202201002"]}]},{"name":"Zhenjiang Science and Technology support project","award":["GJ2021015"],"award-info":[{"award-number":["GJ2021015"]}]},{"name":"Zhenjiang Science and Technology support project","award":["CARS-40-k01"],"award-info":[{"award-number":["CARS-40-k01"]}]},{"name":"Zhenjiang Science and Technology support project","award":["SJCX22_1991"],"award-info":[{"award-number":["SJCX22_1991"]}]},{"name":"China Agriculture Research Systems","award":["2021YFE0111100"],"award-info":[{"award-number":["2021YFE0111100"]}]},{"name":"China Agriculture Research Systems","award":["KY202201002"],"award-info":[{"award-number":["KY202201002"]}]},{"name":"China Agriculture Research Systems","award":["GJ2021015"],"award-info":[{"award-number":["GJ2021015"]}]},{"name":"China Agriculture Research Systems","award":["CARS-40-k01"],"award-info":[{"award-number":["CARS-40-k01"]}]},{"name":"China Agriculture Research Systems","award":["SJCX22_1991"],"award-info":[{"award-number":["SJCX22_1991"]}]},{"name":"the Postgraduate Research and Practice Innovation Program of Jiangsu Province","award":["2021YFE0111100"],"award-info":[{"award-number":["2021YFE0111100"]}]},{"name":"the Postgraduate Research and Practice Innovation Program of Jiangsu Province","award":["KY202201002"],"award-info":[{"award-number":["KY202201002"]}]},{"name":"the Postgraduate Research and Practice Innovation Program of Jiangsu Province","award":["GJ2021015"],"award-info":[{"award-number":["GJ2021015"]}]},{"name":"the Postgraduate Research and Practice Innovation Program of Jiangsu Province","award":["CARS-40-k01"],"award-info":[{"award-number":["CARS-40-k01"]}]},{"name":"the Postgraduate Research and Practice Innovation Program of Jiangsu Province","award":["SJCX22_1991"],"award-info":[{"award-number":["SJCX22_1991"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The intestine is highly vulnerable to various factors and has been proposed as a promising determinant for poultry health. Phytogenic or plant-derived feed additives can be used to help improve intestinal health. In this study, we aimed to investigate the effects of DNJ on the antioxidative parameters, including malondialdehyde (MDA), total superoxide dismutase (T-SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and inflammatory cytokines (IL-6, IL-1\u03b2, and TNF-\u03b1), in plasma and intestinal tissues using layers supplemented with or without the DNJ extract of mulberry leaves (DNJ-E) via the ELISA method. A total of 192 healthy Hy-Line Brown layers, aged 47 weeks old, were used to conduct a 56-day study. All hens were randomly separated into four groups as follows: a basal diet containing 0 mg\/kg DNJ-E(CON), 50 mg\/kg, 100 mg\/kg, and 150 mg\/kg DNJ-E. Furthermore, the potential mechanism by which DNJ influences intestinal function was also investigated in in vitro cultured intestinal epithelium cells (IEC) with quantification methods including the use of a cell counting kit-8 (CCK8), ELISA, qRT-PCR, and ROS detection. The results showed that CAT in plasma significantly increased following 50 mg\/kg DNJ-E supplementation. Moreover, 50 mg\/kg DNJ-E supplementation was associated with increases in T-SOD in the jejunum and ileum. However, there was no significant difference in inflammatory cytokines between groups in in vivo experiments. Subsequent in vitro IEC studies revealed that cell viability increased significantly following 5 \u00b5M and 10 \u00b5M DNJ treatments while decreasing significantly following 20 \u00b5M DNJ treatment. Antioxidative parameters improved at 5 \u00b5M and 10 \u00b5M DNJ concentrations. However, there were no ameliorative effects on antioxidant parameters observed under 20 \u00b5M DNJ treatment. The expression levels of Nrf2 mRNA increased significantly under DNJ treatment. DNJ treatment was associated with significant changes in the expression of genes of inflammatory cytokines. In conclusion, our study revealed that DNJ could improve oxidative stress and inflammation responses in the chicken intestine. These findings provide a theoretical reference for the development of functional feed additives that regulate intestinal health and lay the foundation for systematically revealing the mechanism of DNJ.<\/jats:p>","DOI":"10.3390\/ani13182830","type":"journal-article","created":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T10:14:14Z","timestamp":1693995254000},"page":"2830","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The Effects of 1-Deoxynojirimycin from Mulberry on Oxidative Stress and Inflammation in Laying Hens and the Direct Effects on Intestine Epithelium Cells In Vitro"],"prefix":"10.3390","volume":"13","author":[{"given":"Mingzhu","family":"Wang","sequence":"first","affiliation":[{"name":"College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8860-6311","authenticated-orcid":false,"given":"Tao","family":"Li","sequence":"additional","affiliation":[{"name":"College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengfeng","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adileidys Ruiz","family":"Barcenas","sequence":"additional","affiliation":[{"name":"Plant Protein and Bionatural Products Research Center, Havana 999075, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris Ramos","family":"Serrano","sequence":"additional","affiliation":[{"name":"Plant Protein and Bionatural Products Research Center, Havana 999075, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0785-3324","authenticated-orcid":false,"given":"Liang","family":"Qu","sequence":"additional","affiliation":[{"name":"Jiangsu Institute of Poultry Science, Yangzhou 225212, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1923-861X","authenticated-orcid":false,"given":"Manman","family":"Shen","sequence":"additional","affiliation":[{"name":"College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China"},{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9477-3383","authenticated-orcid":false,"given":"Weiguo","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"60","DOI":"10.3389\/fvets.2019.00060","article-title":"Oxidative Stress in the Poultry Gut: Potential Challenges and Interventions","volume":"6","author":"Mishra","year":"2019","journal-title":"Front. Vet. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1986","DOI":"10.2527\/jas.2011-3949","article-title":"Acute heat stress impairs performance parameters and induces mild intestinal enteritis in broiler chickens: Role of acute hypothalamic-pituitary-adrenal axis activation","volume":"90","author":"Rodrigues","year":"2012","journal-title":"J. Anim. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"102472","DOI":"10.1016\/j.psj.2022.102472","article-title":"Protective effects of Fagopyrum dibotrys on oxidized oil-induced oxidative stress, intestinal barrier impairment, and altered cecal microbiota in broiler chickens","volume":"102","author":"Chen","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Moita, V.H.C., and Kim, S.W. (2022). Nutritional and Functional Roles of Phytase and Xylanase Enhancing the Intestinal Health and Growth of Nursery Pigs and Broiler Chickens. Animals, 12.","DOI":"10.3390\/ani12233322"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6939","DOI":"10.1039\/D1TB00720C","article-title":"Superoxide dismutase nanozymes: An emerging star for anti-oxidation","volume":"9","author":"Zhao","year":"2021","journal-title":"J. Mater. Chem. B"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"179","DOI":"10.7883\/yoken.JJID.2018.277","article-title":"Effects of Antimicrobial Administration on the Prevalence of Antimicrobial-Resistant Escherichia coli in Broiler Flocks","volume":"72","author":"Suzuki","year":"2019","journal-title":"Jpn. J. Infect. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.aninu.2015.12.008","article-title":"Effects of mulberry leaf flavonoid and resveratrol on methane emission and nutrient digestion in sheep","volume":"1","author":"Chen","year":"2015","journal-title":"Anim. Nutr."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"339","DOI":"10.17221\/166\/2021-CJAS","article-title":"Effect of dietary supplementation with mulberry and moringa leaves on the chicken reproductive performance","volume":"67","author":"Shen","year":"2022","journal-title":"Crech J. Anim. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Hassan, F.U., Arshad, M.A., Li, M., Rehman, M.S., Loor, J.J., and Huang, J. (2020). Potential of Mulberry Leaf Biomass and Its Flavonoids to Improve Production and Health in Ruminants: Mechanistic Insights and Prospects. Animals, 10.","DOI":"10.3390\/ani10112076"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"6098","DOI":"10.3168\/jds.2017-13957","article-title":"The influence of mulberry leaf flavonoids and Candida tropicalis on antioxidant function and gastrointestinal development of preweaning calves challenged with Escherichia coli O141:K99","volume":"101","author":"Wang","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"9335","DOI":"10.1007\/s00253-019-10191-9","article-title":"An overview of the biological production of 1-deoxynojirimycin: Current status and future perspective","volume":"103","author":"Zhang","year":"2019","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"8774","DOI":"10.1021\/acs.jafc.0c01668","article-title":"Dietary 5,6,7-Trihydroxy-flavonoid Aglycones and 1-Deoxynojirimycin Synergistically Inhibit the Recombinant Maltase-Glucoamylase Subunit of \u03b1-Glucosidase and Lower Postprandial Blood Glucose","volume":"68","author":"Dong","year":"2020","journal-title":"J. Agric. Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"628","DOI":"10.2174\/0929867327666200114112728","article-title":"1-Deoxynojirimycin and its Derivatives: A Mini Review of the Literature","volume":"28","author":"Wang","year":"2021","journal-title":"Curr. Med. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2189","DOI":"10.1021\/acs.jnatprod.9b00205","article-title":"Effect of 1-Deoxynojirimycin Isolated from Mulberry Leaves on Glucose Metabolism and Gut Microbiota in a Streptozotocin-Induced Diabetic Mouse Model","volume":"82","author":"Hu","year":"2019","journal-title":"J. Nat. Prod."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"113648","DOI":"10.1016\/j.biopha.2022.113648","article-title":"1-Deoxynojirimycin attenuates septic cardiomyopathy by regulating oxidative stress, apoptosis, and inflammation via the JAK2\/STAT6 signaling pathway","volume":"155","author":"Jiang","year":"2022","journal-title":"Biomed. Pharmacother."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"105269","DOI":"10.1016\/j.antiviral.2022.105269","article-title":"Antiviral effects of deoxynojirimycin (DNJ)-based iminosugars in dengue virus-infected primary dendritic cells","volume":"199","author":"Perera","year":"2022","journal-title":"Antivir. Res."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Liu, Q., Li, X., Li, C., Zheng, Y., Wang, F., Li, H., and Peng, G. (2016). 1-Deoxynojirimycin Alleviates Liver Injury and Improves Hepatic Glucose Metabolism in db\/db Mice. Molecules, 21.","DOI":"10.3390\/molecules21030279"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"5858","DOI":"10.1016\/j.psj.2020.07.048","article-title":"1-Deoxynojirimycin from mulberry leaves changes gut digestion and microbiota composition in geese","volume":"99","author":"Hou","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_19","first-page":"49","article-title":"Effects of Dietary Supplementation 1-Deoxynojirimycin from Mulberry Leaf on Performance, Egg Quality, Health of 49-56 Weeks Old Layers","volume":"44","author":"Feng","year":"2023","journal-title":"Feed Ind."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Ghiselli, F., Rossi, B., Felici, M., Parigi, M., Tosi, G., Fiorentini, L., Massi, P., Piva, A., and Grilli, E. (2021). Isolation, culture, and characterization of chicken intestinal epithelial cells. BMC Mol. Cell Biol., 22.","DOI":"10.1186\/s12860-021-00349-7"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1111\/asj.12337","article-title":"Evaluation of embryonic age and the effects of different proteases on the isolation and primary culture of chicken intestinal epithelial cells in vitro","volume":"86","author":"Yuan","year":"2015","journal-title":"Anim. Sci. J."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Novotn\u00fd, J., Hor\u00e1kov\u00e1, L., \u0158ih\u00e1\u010dek, M., Z\u00e1le\u0161\u00e1kov\u00e1, D., \u0160\u0165astn\u00edk, O., Mrkvicov\u00e1, E., Kumb\u00e1r, V., and Pavlata, L. (2023). Effect of Different Feed Particle Size on Gastrointestinal Tract Morphology, Ileal Digesta Viscosity, and Blood Biochemical Parameters as Markers of Health Status in Broiler Chickens. Animals, 13.","DOI":"10.3390\/ani13152532"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"110715","DOI":"10.1016\/j.ecoenv.2020.110715","article-title":"Copper induces oxidative stress with triggered NF-\u03baB pathway leading to inflammatory responses in immune organs of chicken","volume":"200","author":"Yang","year":"2020","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1026199","DOI":"10.3389\/fphar.2022.1026199","article-title":"Selenium-enriched yeast modulates the metal bioaccumulation, oxidant status, and inflammation in copper-stressed broiler chickens","volume":"13","author":"Habotta","year":"2022","journal-title":"Front. Pharmacol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/j.jpha.2022.08.001","article-title":"Rabdosia serra alleviates dextran sulfate sodium salt-induced colitis in mice through anti-inflammation, regulating Th17\/Treg balance, maintaining intestinal barrier integrity, and modulating gut microbiota","volume":"12","author":"Li","year":"2022","journal-title":"J. Pharm. Anal."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"107989","DOI":"10.1016\/j.intimp.2021.107989","article-title":"Resveratrol protects intestinal integrity, alleviates intestinal inflammation and oxidative stress by modulating AhR\/Nrf2 pathways in weaned piglets challenged with diquat","volume":"99","author":"Xun","year":"2021","journal-title":"Int. Immunopharmacol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Sun, L., Xu, G., Dong, Y., Li, M., Yang, L., and Lu, W. (2020). Quercetin Protects Against Lipopolysaccharide-Induced Intestinal Oxidative Stress in Broiler Chickens through Activation of Nrf2 Pathway. Molecules, 25.","DOI":"10.3390\/molecules25051053"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1111\/jpn.12634","article-title":"Effect of Moringa oleifera leaf powder supplementation on growth performance and intestinal morphology in broiler chickens","volume":"101","author":"Khan","year":"2017","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Anagnostopoulos, E.C., Brouklogiannis, I.P., Griela, E., Paraskeuas, V.V., and Mountzouris, K.C. (2023). Phytogenic Effects on Layer Production Performance and Cytoprotective Response in the Duodenum. Animals, 13.","DOI":"10.3390\/ani13020294"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"5869","DOI":"10.1021\/jf062680g","article-title":"Food-grade mulberry powder enriched with 1-deoxynojirimycin suppresses the elevation of postprandial blood glucose in humans","volume":"55","author":"Kimura","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"21700","DOI":"10.3390\/molecules201219794","article-title":"1-Deoxynojirimycin Alleviates Insulin Resistance via Activation of Insulin Signaling PI3K\/AKT Pathway in Skeletal Muscle of db\/db Mice","volume":"20","author":"Liu","year":"2015","journal-title":"Molecules"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Gao, K., Zheng, C., Wang, T., Zhao, H., Wang, J., Wang, Z., Zhai, X., Jia, Z., Chen, J., and Zhou, Y. (2016). 1-Deoxynojirimycin: Occurrence, Extraction, Chemistry, Oral Pharmacokinetics, Biological Activities and In Silico Target Fishing. Molecules, 21.","DOI":"10.3390\/molecules21111600"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"569","DOI":"10.3389\/fphar.2019.00569","article-title":"1-Deoxynojirimycin in Mulberry (Morus indica L.) Leaves Ameliorates Stable Angina Pectoris in Patients With Coronary Heart Disease by Improving Antioxidant and Anti-inflammatory Capacities","volume":"10","author":"Ma","year":"2019","journal-title":"Front. Pharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"372","DOI":"10.3389\/fphar.2018.00372","article-title":"1-Deoxynojirimycin (DNJ) Ameliorates Indomethacin-Induced Gastric Ulcer in Mice by Affecting NF-kappaB Signaling Pathway","volume":"9","author":"Piao","year":"2018","journal-title":"Front. Pharmacol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"114483","DOI":"10.1016\/j.jep.2021.114483","article-title":"Morus alba L. (Sangzhi) alkaloids (SZ-A) exert anti-inflammatory effects via regulation of MAPK signaling in macrophages","volume":"280","author":"Cao","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.biocel.2006.07.001","article-title":"Free radicals and antioxidants in normal physiological functions and human disease","volume":"39","author":"Valko","year":"2007","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1637\/11524-110216-Reg.1","article-title":"Establishment of an In Vitro Intestinal Epithelial Cell Culture Model of Avian Origin","volume":"61","author":"Kaiser","year":"2017","journal-title":"Avian Dis."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.exger.2014.03.025","article-title":"1-Deoxynojirimycin attenuates high glucose-accelerated senescence in human umbilical vein endothelial cells","volume":"55","author":"Shuang","year":"2014","journal-title":"Exp. Gerontol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1089\/ars.2010.3301","article-title":"The Nrf2-Keap1-ARE signaling pathway: The regulation and dual function of Nrf2 in cancer","volume":"13","author":"Zhang","year":"2010","journal-title":"Antioxid. Redox Signal."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Thiruvengadam, M., Venkidasamy, B., Subramanian, U., Samynathan, R., Ali Shariati, M., Rebezov, M., Girish, S., Thangavel, S., Dhanapal, A.R., and Fedoseeva, N. (2021). Bioactive Compounds in Oxidative Stress-Mediated Diseases: Targeting the NRF2\/ARE Signaling Pathway and Epigenetic Regulation. Antioxidants, 10.","DOI":"10.3390\/antiox10121859"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"133","DOI":"10.14348\/molcells.2023.0028","article-title":"Molecular Basis of the KEAP1-NRF2 Signaling Pathway","volume":"46","author":"Suzuki","year":"2023","journal-title":"Mol. Cells"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/18\/2830\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:46:08Z","timestamp":1760129168000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/18\/2830"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,6]]},"references-count":41,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["ani13182830"],"URL":"https:\/\/doi.org\/10.3390\/ani13182830","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,6]]}}}