{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T18:04:31Z","timestamp":1781114671542,"version":"3.54.1"},"reference-count":41,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T00:00:00Z","timestamp":1696550400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007159","name":"ANID Fondecyt program","doi-asserted-by":"publisher","award":["11190569"],"award-info":[{"award-number":["11190569"]}],"id":[{"id":"10.13039\/501100007159","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This work aimed to evaluate the gene expression of amino acids (AA) and fatty acids (FA) sensors in the gastrointestinal tract (GIT) of chickens at two different ages (7 and 26 days post-hatch). Sixteen broilers (Ross 308) were selected, and ten sections of the GIT, including upper (tongue base, upper palate, crop, proventriculus), middle (gizzard, duodenum, jejunum, ileum), and lower GIT section (cecum, colon) were collected for analysis. Relative gene expression of AA (T1R1, T1R3, mGluR1, mGluR4, CaSR, GPR139, GPRC6A, GPR92) and FA (FFAR2, FFAR3, FFAR4) sensors were assessed using qPCR. The statistical model included age, GIT section, and gene. In addition, the correlations between gene expressions were calculated. At day 7, a significantly (p = 0.004) higher expression of AA sensors in the oral cavity and FA sensors in the lower GIT section (i.e., cecum and colon) compared to the middle section was recorded. A higher expression of AA compared to FA sensors was detected at the upper GIT section in 7 (p &lt; 0.001) and 26-day-old chickens (p = 0.026). Thus, at day 7, AA sensors were predominantly (p &lt; 0.05) expressed in the upper GIT section (mainly oral cavity), while FA sensors were mainly expressed in the lower GIT section, at cecum (FFR2 and 4) or colon (FFAR3). These results may indicate that in early life, both ends of the GIT are fundamental for feed intake (oral cavity) and development of the microbiota (cecum and colon). In contrast, at 26 days of age, the results showed the emergence of both AA and FA sensors in the jejunum, presumably indicating the essential role of the jejunum in the digestion absorption of nutrients and the signaling to the brain (gut-brain axis) through the enteroendocrine system. Significant positive correlations were observed between T1R1 and T1R3 (r = 0.85, p &lt; 0.001), CaSR and T1R1 (r = 0.78, p &lt; 0.001), CaSR and T1R3 (r = 0.45, p &lt; 0.050), and mGluR1 and FFAR3 (r = 0.46, p &lt; 0.050). It is concluded that the gene expression is greater in the oral cavity for AA sensors and the lower gut for FA sensors. On day 26, the role of jejunum regarding nutrient sensing is highlighted.<\/jats:p>","DOI":"10.3390\/ani13193120","type":"journal-article","created":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T08:29:12Z","timestamp":1696580952000},"page":"3120","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The Expression of Amino Acid and Fatty Acid Receptors Show an Age-Dependent Pattern Involving Oral Cavity, Jejunum and Lower Gut Sensing in Broiler Chickens"],"prefix":"10.3390","volume":"13","author":[{"given":"Paloma","family":"Cordero","sequence":"first","affiliation":[{"name":"Programa de Doctorado en Ciencias Silvoagropecuarias y Veterinarias, Campus Sur, Universidad de Chile, Santiago 8820808, Chile"},{"name":"Departamento de Fomento de la Producci\u00f3n Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago 8820808, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisca","family":"D\u00edaz-Avil\u00e9s","sequence":"additional","affiliation":[{"name":"Departamento de Fomento de la Producci\u00f3n Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago 8820808, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina","family":"Torres","sequence":"additional","affiliation":[{"name":"Laboratorio de Patolog\u00eda Aviar, Facultad de Ciencias Veterinaria y Pecuarias, Universidad de Chile, Santiago 8820808, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2264-4128","authenticated-orcid":false,"given":"Miguel","family":"Guzm\u00e1n","sequence":"additional","affiliation":[{"name":"Laboratorio de Patolog\u00eda Aviar, Facultad de Ciencias Veterinaria y Pecuarias, Universidad de Chile, Santiago 8820808, Chile"},{"name":"Nucleus of Applied Research in Veterinary and Agronomic Sciences, Faculty of Veterinary Medicine and Agronomy, Universidad de las Am\u00e9ricas, Santiago 7500975, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahram","family":"Niknafs","sequence":"additional","affiliation":[{"name":"Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9073-9946","authenticated-orcid":false,"given":"Eugeni","family":"Roura","sequence":"additional","affiliation":[{"name":"Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1763-5630","authenticated-orcid":false,"given":"Sergio A.","family":"Guzm\u00e1n-Pino","sequence":"additional","affiliation":[{"name":"Departamento de Fomento de la Producci\u00f3n Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago 8820808, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1071\/AN16775","article-title":"Physiological and metabolic control of diet selecWtion. Festschrift for Prof John L Black: Celebrating 45 years of Excellence in Animal Production Science and Application","volume":"58","author":"Roura","year":"2018","journal-title":"Anim. Prod. Sci."},{"key":"ref_2","unstructured":"Zufall, F., and Munger, S. (2016). Book Chemosensory Transduction, Elsiever."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1152\/ajpendo.00036.2019","article-title":"Metabolic control via nutrient-sensing mechanisms: Role of taste receptors and the gut-brain neuroendocrine axis","volume":"317","author":"Raka","year":"2019","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anifeedsci.2012.11.001","article-title":"The avian taste system: Potential implications in poultry nutrition","volume":"180","author":"Roura","year":"2013","journal-title":"Anim. Feed. Sci. Technol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1708","DOI":"10.1172\/JCI40794","article-title":"Loss of enteroendocrine cells in mice alters lipid absorption and glucose homeostasis and impairs postnatal survival","volume":"120","author":"Mellitzer","year":"2010","journal-title":"J. Clin. Investig."},{"key":"ref_6","unstructured":"Van Der Wielen, N. (2016). Intestinal Nutrient Sensing: A Gut Feeling for Food. [Ph.D. Thesis, Wageningen University]."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1017\/S0954422418000100","article-title":"Nutrient sensing, taste, and feed intake in avian species","volume":"31","author":"Niknafs","year":"2016","journal-title":"Nutr. Res. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1928","DOI":"10.3382\/ps\/pev152","article-title":"Bitter, sweet, and umami taste receptors and downstream signaling effectors: Expression in the embryonic and growing chicken gastrointestinal tract","volume":"94","author":"Druyan","year":"2015","journal-title":"Poult. Sci. J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1126\/science.1255097","article-title":"Evolution of sweet taste perception in hummingbirds by transformation of the ancestral umami receptor","volume":"345","author":"Baldwin","year":"2014","journal-title":"Science"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1093\/gbe\/evv072","article-title":"Expanding duplication of free fatty acid receptor-2 (GPR43) genes in the chicken genome","volume":"7","author":"Meslin","year":"2015","journal-title":"Genome Biol. Evol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.bbrc.2015.09.025","article-title":"Expression of multiple umami taste receptors in oral and gastrointestinal tissues, and umami taste synergism in chickens","volume":"466","author":"Yoshida","year":"2015","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6035","DOI":"10.1073\/pnas.1520843113","article-title":"Taste cell-expressed \u03b1-glucosidase enzymes contribute to gustatory responses to disaccharides","volume":"113","author":"Sukumarana","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Hui, Q., Zhao, X., Lu, P., Liu, S., Nyachoti, M., Karmin, O., and Yang, C. (2021). Molecular distribution and localization of extracellular calcium-sensing Receptor (CASR) and Vitamin D Receptor (VDR) at three different laying stages in laying hens (Gallus Gallus Domesticus). Genet. Mol. Biol., 100.","DOI":"10.1016\/j.psj.2021.101060"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Da Silva, E.C., De Jager, N., Burgos, W., Reverter, A., Perez-Enciso, M., and Roura, E. (2014). Characterization of the Porcine GPCR Nutrient Sensor and Taste Receptor Gene Repertoire across international and local domestic breeds and wild populations. BMC Genom., 15.","DOI":"10.1186\/1471-2164-15-1057"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Palomar, M., Soler, M., Roura, E., Sala, R., Piquer, O., and Garc\u00e9s-Narro, C. (2020). Degree of saturation and free fatty acid content of fats determine dietary preference in hens. Animals, 10.","DOI":"10.3390\/ani10122437"},{"key":"ref_16","unstructured":"Aviagen (2022, February 06). Ross 308 Broiler: Nutrition Specifications. Available online: http:\/\/eu.aviagen.com\/assets\/Tech_Center\/Ross_Broiler\/RossBroilerNutritionSpecs2019-EN.pdf."},{"key":"ref_17","unstructured":"OIE (2022, March 02). World Organization for Animal Health. Animal Sacrifice. Health Code for Terrestrial Animals 2019, 7.5(7.5.1). Available online: https:\/\/doc.woah.org\/dyn\/portal\/index.xhtml?page=alo&aloId=38680&espaceId=100."},{"key":"ref_18","first-page":"1","article-title":"Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes","volume":"3","author":"Vandosempele","year":"2002","journal-title":"Genome Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1146\/annurev-physiol-021317-121332","article-title":"Chemoreceptores in the gut","volume":"80","author":"Steensels","year":"2018","journal-title":"Annu Rev. Physiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1146\/annurev-animal-030117-014740","article-title":"Nutrient-sensing biology in mammals and birds","volume":"6","author":"Roura","year":"2018","journal-title":"Annu. Rev. Anim. Biosci."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Montmayeur, J., and le Coutre, J. (2010). Book Fat Detection: Taste, Texture, and Post Ingestive Effects, CRP Press.","DOI":"10.1201\/9781420067767"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.bbrc.2016.09.064","article-title":"Distribution of alpha-Gustducin and Vimentin in premature and mature taste buds in chickens","volume":"479","author":"Venkatesan","year":"2016","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_23","unstructured":"Dong, B. (2016). Molecular Characterization and Expression of Umami Receptors T1R1\/T1R3 in Broiler Chickens. [Master\u2019s Thesis, University of Manitoba]."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"261","DOI":"10.3389\/fphys.2015.00261","article-title":"Analysis of the protein related receptor GPR92 in G-cells","volume":"6","author":"Rettenberger","year":"2015","journal-title":"Front. Physiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1017\/S0029665112000110","article-title":"Nutrient sensing and signaling by the gut","volume":"71","author":"Rasoamanana","year":"2012","journal-title":"Proc. Nutr. Soc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1079\/WPS20040017","article-title":"Characteristics of the gastrointestinal microbial communities, with special reference to the chicken","volume":"60","author":"Apajalahti","year":"2014","journal-title":"Worlds Poult. Sci. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"671","DOI":"10.3382\/ps.2012-02822","article-title":"Bacterial census of poultry intestinal microbiome","volume":"92","author":"Wei","year":"2013","journal-title":"Poult. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1111\/dom.12340","article-title":"Gut microbiota, nutrient sensing, and energy balance","volume":"16","author":"Duca","year":"2014","journal-title":"Diabetes Obes. Metab."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1080\/17450390701556817","article-title":"Indigenous bacteria and bacterial metabolic products in the gastrointestinal tract of broiler chickens","volume":"61","author":"Rehman","year":"2007","journal-title":"Arch. Anim. Nutr."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1007\/s00418-018-1746-x","article-title":"Enteroendocrine cell diversity investigated at the cellular and subcellular level: The need for a new classification scheme","volume":"150","author":"Fothergill","year":"2018","journal-title":"Histochem. Cell. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/s00418-013-1080-2","article-title":"Gustatory sensory cells express a receptor responsive to protein breakdown products (GPR92)","volume":"140","author":"Haid","year":"2013","journal-title":"Histoch. Cell Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"23589","DOI":"10.1074\/jbc.M603670200","article-title":"GPR92 as a new G12\/13- and Gq-coupled lysophosphatidic acid receptor that increases cAMP, LPA5","volume":"281","author":"Lee","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_33","first-page":"249","article-title":"Expression and tissue distribution of extracellular calcium-sensing receptor (CaSR) mRNA in chickens","volume":"34","author":"Deng","year":"2010","journal-title":"Turk. J. Vet. Anim. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"104604","DOI":"10.1016\/j.phrs.2019.104604","article-title":"Free fatty acid receptors as new potential therapeutic target in inflammatory bowel diseases","volume":"152","author":"Bartoszek","year":"2020","journal-title":"Pharmacol. Res."},{"key":"ref_35","first-page":"93","article-title":"The avian cecum: A review","volume":"107","author":"Clench","year":"1995","journal-title":"Wilson. Bull."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Rychlik, I. (2020). Composition and function of chicken gut microbiota. Animals, 10.","DOI":"10.3390\/ani10010103"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"11312","DOI":"10.1074\/jbc.M211609200","article-title":"The Orphan G protein-coupled receptors GPR41 and GPR43 are activated by propionate and other short chain carboxylic acids","volume":"278","author":"Brown","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"85","DOI":"10.3389\/fendo.2014.00085","article-title":"The SCFA Receptor GPR43 and energy metabolism","volume":"5","author":"Kimura","year":"2014","journal-title":"Front. Endocrinol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.bcp.2016.03.017","article-title":"Metabolism meets immunity: The role of free fatty acid receptors in the immune system","volume":"114","author":"Milligan","year":"2016","journal-title":"Biochem. Pharmacol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1038\/cti.2016.17","article-title":"Regulation of immune cell function by short-chain fatty acids","volume":"5","author":"Fachi","year":"2016","journal-title":"Clin. Transl. Immunol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1152\/ajpgi.00074.2012","article-title":"Sensing of amino acids by the gut-expressed taste receptor T1R1-T1R3 stimulates CCK secretion","volume":"304","author":"Daly","year":"2013","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/19\/3120\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:02:05Z","timestamp":1760130125000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/19\/3120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,6]]},"references-count":41,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["ani13193120"],"URL":"https:\/\/doi.org\/10.3390\/ani13193120","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,6]]}}}