{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:26:13Z","timestamp":1789626373581,"version":"build-2803163510"},"reference-count":36,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T00:00:00Z","timestamp":1697846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Centre for Research and Development, Poland","award":["BIOSTRATEG2\/297910\/12\/NCBR\/2016"],"award-info":[{"award-number":["BIOSTRATEG2\/297910\/12\/NCBR\/2016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>In this study, a morphometric analysis of morphological changes in the layers of the small intestine (duodenum and jejunum) and liver occurring during the hatching period (week 0) and postnatal development (weeks 1, 3, 6, and 8) was performed in geese. For this purpose, the staining of samples obtained from tissues collected from geese after culling was carried out. Staining was performed using the Goldner method to visualize all layers of the intestine for morphometric measurements. Our analysis focused mainly on traits such as the thickness of the mucosal, submucosal, and muscular layers, as well as traits related to intestinal absorption, such as the height and width of intestinal villi and crypts. Additionally, we also took into account the number of mononuclear and binucleate hepatocytes and other cells present in the liver. After analyzing the results, an increase in most traits was found during the development of the animals, with slight differences between the sections of the duodenum and jejunum. An interesting phenomenon was also noticed\u2014the greatest increase in most traits was observed between the 3rd and 6th week of life, which coincides with the time of feed change. We hope that our work will highlight how important the digestive system is for birds because research on this topic is limited.<\/jats:p>","DOI":"10.3390\/ani13203292","type":"journal-article","created":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T12:59:01Z","timestamp":1697893141000},"page":"3292","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Morphometric Analysis of Developmental Alterations in the Small Intestine of Goose"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2810-6280","authenticated-orcid":false,"given":"Ligia","family":"Hi\u017cewska","sequence":"first","affiliation":[{"name":"Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5630-4968","authenticated-orcid":false,"given":"Cezary","family":"Osiak-Wicha","sequence":"additional","affiliation":[{"name":"Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7574-2653","authenticated-orcid":false,"given":"Ewa","family":"Tomaszewska","sequence":"additional","affiliation":[{"name":"Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4118-6302","authenticated-orcid":false,"given":"Siemowit","family":"Muszy\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Faculty of Environmental Biology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5873-9666","authenticated-orcid":false,"given":"Piotr","family":"Dobrowolski","sequence":"additional","affiliation":[{"name":"Department of Functional Anatomy and Cytobiology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, Akademicka St. 19, 20-033 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9417-5965","authenticated-orcid":false,"given":"Krzysztof","family":"Andres","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Cracow, Mickiewicza Alley 24\/28, 30-059 Cracow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics, Breeding and Ethology, Faculty of Animal Sciences, University of Agriculture in Cracow, Mickiewicza Alley 24\/28, 30-059 Cracow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3643-3524","authenticated-orcid":false,"given":"Marcin B.","family":"Arciszewski","sequence":"additional","affiliation":[{"name":"Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ravindran, V., and Abdollahi, M.R. (2021). Nutrition and Digestive Physiology of the Broiler Chick: State of the Art and Outlook. Animals, 11.","DOI":"10.3390\/ani11102795"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.3382\/ps\/pey461","article-title":"Enrichment of the amnion with threonine in chicken embryos affects the small intestine development, ileal gene expression and performance of broilers between 1 and 21 days of age","volume":"98","author":"Ferket","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/S1055-937X(99)80036-X","article-title":"Avian Gastrointestinal Anatomy and Physiology","volume":"8","author":"Klasing","year":"1999","journal-title":"Semin. Avian Exot. Pet Med."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Calhoun, M. (1954). Microscopic Anatomy of the Digestive System, Iowa State College Press.","DOI":"10.31274\/isudp.1954.84"},{"key":"ref_5","unstructured":"Denbow, D.M. (2015). Sturkie\u2032s Avian Physiology, Academic Press. [6th ed.]."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/imr.12182","article-title":"The mucus and mucins of the goblet cells and enterocytes provide the first defense line of the gastrointestinal tract and interact with the immune system","volume":"260","author":"Pelaseyed","year":"2014","journal-title":"Immunol. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"757","DOI":"10.2478\/aoas-2020-0099","article-title":"Supplementation of bile acids and lipase in broiler diets for better nutrient utilization and performance: Potential effects and future implications\u2014A review","volume":"21","author":"Arshad","year":"2021","journal-title":"Ann. Anim. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"641","DOI":"10.2478\/aoas-2022-0083","article-title":"Fat Digestion and Metabolism: Effect of Different Fat Sources and Fat Mobilisers in Broilers\u2019 Diet on Growth Performance and Physiological Parameters\u2014A Review","volume":"23","author":"Shoaib","year":"2023","journal-title":"Ann. Anim. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Zaefarian, F., Abdollahi, M.R., Cowieson, A., and Ravindran, V. (2019). Avian Liver: The Forgotten Organ. Animals, 9.","DOI":"10.3390\/ani9020063"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.3382\/ps.0611225","article-title":"The Anatomy of the Avian Digestive Tract as Related to Feed Utilization","volume":"61","author":"Turk","year":"1982","journal-title":"Poult. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1053\/ax.2000.7138","article-title":"Avian liver disease: Etiology and pathogenesis","volume":"9","author":"Davies","year":"2000","journal-title":"Semin. Avian Exot. Pet Med."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Schmidt, R.E., Reavill, D.R., Phalen, D.N., Liver, W., Schmidt, R.E., Reavill, D.R., and Phalen, D.N. (2003). Pathology of Pet and Aviary Birds, Iowa State Press.","DOI":"10.1002\/9780470376836"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"842","DOI":"10.3382\/ps.2010-00939","article-title":"Ultrastructural development of the small intestinal mucosa in the embryo and turkey poult: A light and electron microscopy study","volume":"90","author":"Ferket","year":"2011","journal-title":"Poult. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2629","DOI":"10.3382\/ps.2008-00163","article-title":"Developmental morphology of the small intestine of African ostrich chicks","volume":"87","author":"Wang","year":"2008","journal-title":"Poult. Sci."},{"key":"ref_15","first-page":"423","article-title":"Morphometric analysis of the small intestine in wild type mice C57BL\/6J\u2014A developmental study","volume":"63","author":"Gulbinowicz","year":"2004","journal-title":"Folia Morphol."},{"key":"ref_16","unstructured":"(2006). The Act of 10 May 2006 on the Scope and Methods of Carrying out the Assessment of the Use and Breeding Value of Animals and the Method of Marking and Identifying Animals for Breeding Purposes. J. Laws, 79, 554."},{"key":"ref_17","unstructured":"The National Research Institute of Animal Production (2021). The Act of 28 December 2021 on the Introduction of Programs for the Conservation of Genetic Resources of Populations of Laying Hens, Geese and Ducks, The National Research Institute of Animal Production. Available online: http:\/\/www.bioroznorodnosc.izoo.krakow.pl\/drob\/dokumenty."},{"key":"ref_18","unstructured":"Jamroz, D. (2005). Standards of Poultry Nutrition, Institute of Physiology and Animal Nutrition. [4th ed.]."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Bancroft, J.D., and Layton, C. (2019). Bancroft\u2019s Theory and Practice of Histological Techniques, Elsevier. Connective and Other Mesenchymal Tissues with Their Stains.","DOI":"10.1016\/B978-0-7020-6864-5.00012-8"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/s102380200018","article-title":"Simple new method to calculate small intestine absorptive surface in the rat","volume":"2","author":"Kisielinski","year":"2002","journal-title":"Clin. Exp. Med."},{"key":"ref_21","first-page":"253","article-title":"Zatorska goose\u2014A subject of parasitological research","volume":"61","author":"Basiaga","year":"2015","journal-title":"Ann. Parasitol."},{"key":"ref_22","first-page":"19","article-title":"Morphological and histochemical study of small intestine in indigenous ducks (Anasplatyrhynchos)","volume":"10","author":"Khaleel","year":"2017","journal-title":"J. Agric. Vet. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"101","DOI":"10.32819\/2020.82014","article-title":"Features of the microscopical structure of ducks\u2019 small intestine in their postnatal period of ontogenesis","volume":"8","author":"Kushch","year":"2020","journal-title":"Theor. Appl. Vet. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1080\/00071660410001730824","article-title":"Posthatch growth of the digestive system in wild and domesticated ducks","volume":"45","author":"Watkins","year":"2004","journal-title":"Br. Poult. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3326","DOI":"10.1038\/ncomms4326","article-title":"The origin of segmentation motor activity in the intestine","volume":"5","author":"Huizinga","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1080\/00071669108417376","article-title":"Growth and development of the digestive organs and some enzymes in broiler chicks after hatching","volume":"32","author":"Nitsan","year":"1991","journal-title":"Br. Poult. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1093\/ps\/84.4.634","article-title":"Antibiotic growth promoters in agriculture: History and mode of action","volume":"84","author":"Dibner","year":"2005","journal-title":"Poult. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1093\/ps\/82.11.1747","article-title":"Morphological, molecular, and functional changes in the chicken small intestine of the late-term embryo","volume":"82","author":"Uni","year":"2003","journal-title":"Poult. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"100960","DOI":"10.1016\/j.animal.2023.100960","article-title":"Developmental changes in tibia and humerus of goose: Morphometric, densitometric, and mechanical analysis","volume":"17","author":"Tomaszewska","year":"2023","journal-title":"Animal"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1079\/WPS20010030","article-title":"Development of the digestive tract of poultry","volume":"57","author":"Sklan","year":"2001","journal-title":"World\u2032s Poult. Sci. J."},{"key":"ref_31","first-page":"83","article-title":"Histological Study of Small Intestine Development in Local Chicken (Gallus gallus domesticus) and Duck (Anas platyrhynchos domesticus)","volume":"8","author":"Othman","year":"2023","journal-title":"Embryos J. Appl. Vet. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"61","DOI":"10.3382\/ps\/peu019","article-title":"Protein source and nutrient density in the diets of male broilers from 8 to 21 d of age: Effects on small intestine morphology","volume":"94","author":"Wang","year":"2015","journal-title":"Poult. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1093\/japr\/7.4.420","article-title":"Practical Aspects of Early Nutrition for Poultry","volume":"7","author":"Lilburn","year":"1998","journal-title":"J. Appl. Poult. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1556\/ABiol.58.2007.1.4","article-title":"Following the goose liver development by means of cross-sectional digital imaging, liver histology and blood biochemical parameters","volume":"58","year":"2007","journal-title":"Acta Biol. Hung."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1080\/03079457.2019.1682119","article-title":"Unravelling fatty liver haemorrhagic syndrome: 2. Inflammation and pathophysiology","volume":"49","author":"Shini","year":"2020","journal-title":"Avian Pathol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"101015","DOI":"10.1016\/j.psj.2021.01.037","article-title":"Assessment of vitamin A requirement of gosling in 0\u201328 d based on growth performance and bone indexes","volume":"100","author":"Liang","year":"2021","journal-title":"Poult. Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/20\/3292\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:09:38Z","timestamp":1760130578000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/20\/3292"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,21]]},"references-count":36,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["ani13203292"],"URL":"https:\/\/doi.org\/10.3390\/ani13203292","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,21]]}}}