{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T15:01:32Z","timestamp":1776697292368,"version":"3.51.2"},"reference-count":37,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T00:00:00Z","timestamp":1699574400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008771","name":"Animal and Plant Quarantine Agency of the Ministry of Agriculture, Food, and Rural Affairs of the Republic of Korea","doi-asserted-by":"publisher","award":["F-1543084-2022-24-03"],"award-info":[{"award-number":["F-1543084-2022-24-03"]}],"id":[{"id":"10.13039\/501100008771","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008771","name":"Animal and Plant Quarantine Agency of the Ministry of Agriculture, Food, and Rural Affairs of the Republic of Korea","doi-asserted-by":"publisher","award":["N-1541767-2010-19-06"],"award-info":[{"award-number":["N-1541767-2010-19-06"]}],"id":[{"id":"10.13039\/501100008771","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Tumors in birds can be caused by a variety of factors such as species, age, sex, virus, chemicals, and environment. In particular, tumors are a major cause of death in long-lived birds such as parrots and zoo birds. A male sandhill crane that was bred for 8 years in a zoo was diagnosed with intrahepatic cholangiocarcinoma (ICC). At necropsy, the liver revealed a multinodular mass of variable colors, and severe cirrhosis and hemorrhages were present. Histologically, ICC was characterized by the presence of both types of ICC: small-duct type and large-duct type. Large-duct-type ICC was distinguished by the presence of multifocal biliary neoplasia, characterized by the diffuse papillary proliferation of columnar cells resembling large cholangiocytes. Small-duct-type ICC was characterized by the presence of non-mucin-producing cuboidal cells such as bile duct cells. In this case, no viral cause was identified from the metagenomic analysis and PCR of ICC; however, a contributing role of Cutibacterium sp. and E. coli identified from the metagenomics could not be excluded. This study is the first to describe the anatomopathological characteristics of ICC in the studied sandhill crane and attempts to determine its potential infectious etiology using metagenomics.<\/jats:p>","DOI":"10.3390\/ani13223469","type":"journal-article","created":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T05:04:56Z","timestamp":1699592696000},"page":"3469","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Intrahepatic Cholangiocarcinoma Identified in a Zoo-Housed Sandhill Crane (Grus canadensis): An Anatomopathological and Metagenomic Study"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8926-2298","authenticated-orcid":false,"given":"Hye-Ryoung","family":"Kim","sequence":"first","affiliation":[{"name":"Avian Disease Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyeon-Su","family":"Kim","sequence":"additional","affiliation":[{"name":"Avian Disease Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong-Kuk","family":"Kwon","sequence":"additional","affiliation":[{"name":"Avian Disease Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,10]]},"reference":[{"key":"ref_1","unstructured":"Kuruvilla, S., Del Tin, J., Pennington, M., and Heron, T. (2017, February 05). Sandhill Crane (Antigone canadensis)\u2013Ecological Profile. Available online: https:\/\/humanwildlifeecology.wordpress.com\/2017\/02\/05\/sandhill-crane-ecological-profile."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Barzen, J., and Ballinger, K. (2017). Sandhill and Whooping Cranes, National Wildlife Research Center.","DOI":"10.32747\/2017.7207736.ws"},{"key":"ref_3","unstructured":"Krapu, G., Ivey, G., and Barzen, J. (2019). Crane Conservation Strategy, International Crane Foundation."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Thomas, N.J., Hunter, D.B., and Atkinson, C.T. (2008). Infectious Diseases of Wild Birds, John Wiley & Sons.","DOI":"10.1002\/9780470344668"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"742","DOI":"10.7589\/0090-3558-46.3.742","article-title":"Survey for antibodies to infectious bursal disease virus serotype 2 in wild Turkeys and Sandhill cranes of Florida, USA","volume":"46","author":"Candelora","year":"2010","journal-title":"J. Wildl. Dis."},{"key":"ref_6","first-page":"569","article-title":"Mycotoxin-induced disease in captive whooping cranes (Grus americana) and sandhill cranes (Grus canadensis)","volume":"26","author":"Olsen","year":"1995","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"112","DOI":"10.7589\/0090-3558-11.1.112","article-title":"AVIAN POX IN FLORIDA SANDHILL CRANES 1","volume":"11","author":"Simpson","year":"1975","journal-title":"J. Wildl. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1111\/j.1550-7408.1981.tb02844.x","article-title":"Parental Development of Eimerian Coccidia in Sandhill and Whopping Cranes 1","volume":"28","author":"Novilla","year":"1981","journal-title":"J. Protozool."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1638\/2020-0180","article-title":"Neoplasia in captive cranes","volume":"52","author":"Hawkins","year":"2021","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_10","first-page":"1215","article-title":"Metastatic cholangiocarcinoma in a Florida sandhill crane","volume":"187","author":"Allen","year":"1985","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Swayne, D.E., Boulianne, M., Logue, C.M., McDougald, L.R., Nair, V., and Suarez, D.L. (2020). Diseases of Poultry, John Wiley & Sons. [14th ed.].","DOI":"10.1002\/9781119371199"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1637\/0005-2086(2007)51[663:DOAPCR]2.0.CO;2","article-title":"Development of a polymerase chain reaction to differentiate avian leukosis virus (ALV) subgroups: Detection of an ALV contaminant in commercial Marek\u2019s disease vaccines","volume":"51","author":"Silva","year":"2007","journal-title":"Avian Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1016\/0042-6822(70)90218-7","article-title":"Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification","volume":"40","author":"Yamamoto","year":"1970","journal-title":"Virology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"315","DOI":"10.2165\/00063030-200822050-00004","article-title":"The impact of PEGylation on biological therapies","volume":"22","author":"Veronese","year":"2008","journal-title":"BioDrugs"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2900","DOI":"10.1093\/nar\/20.11.2900","article-title":"A random-PCR method (rPCR) to construct whole cDNA library from low amounts of RNA","volume":"20","author":"Froussard","year":"1992","journal-title":"Nucleic Acids Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt removes adapter sequences from high-throughput sequencing reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1101\/gr.213959.116","article-title":"metaSPAdes: A new versatile metagenomic assembler","volume":"27","author":"Nurk","year":"2017","journal-title":"Genome Res."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Wood, D.E., Lu, J., and Langmead, B. (2019). Improved metagenomic analysis with Kraken 2. Genome Biol., 20.","DOI":"10.1186\/s13059-019-1891-0"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1080\/03079459994948","article-title":"Unusual presentation of an Amazon parrot (Amazona species) with hepatocellular carcinoma","volume":"28","author":"Freeman","year":"1999","journal-title":"Avian Pathol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1080\/03079457.2010.493553","article-title":"Combined hepatocellular-cholangiocarcinoma in a lesser flamingo (Phoenicopterus minor)","volume":"39","author":"Degernes","year":"2010","journal-title":"Avian Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1080\/03079457808418309","article-title":"Some hepatic neoplasms in non-domesticated birds","volume":"7","author":"Wadsworth","year":"1978","journal-title":"Avian Pathol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1080\/03079459308418905","article-title":"Pathological observations of hepatic tumours in ducks","volume":"22","author":"Ling","year":"1993","journal-title":"Avian Pathol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"857140","DOI":"10.3389\/fmed.2022.857140","article-title":"Up-to-date pathologic classification and molecular characteristics of intrahepatic cholangiocarcinoma","volume":"9","author":"Chung","year":"2022","journal-title":"Front. Med."},{"key":"ref_25","unstructured":"(2019). WHO Classification of Tumours. Digestive System Tumours, International Agency for Research on Cancer. [5th ed.]."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1876","DOI":"10.1002\/hep.25595","article-title":"Histological diversity in cholangiocellular carcinoma reflects the different cholangiocyte phenotypes","volume":"55","author":"Komuta","year":"2012","journal-title":"Hepatology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1002\/hep.510300614","article-title":"The canals of Hering and hepatic stem cells in humans","volume":"30","author":"Theise","year":"1999","journal-title":"Hepatology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"100226","DOI":"10.1016\/j.jhepr.2021.100226","article-title":"Cell of origin in biliary tract cancers and clinical implications","volume":"3","author":"Moeini","year":"2021","journal-title":"JHEP Rep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"S56","DOI":"10.1002\/hep.22962","article-title":"Hepatitis B and hepatocellular carcinoma","volume":"49","year":"2009","journal-title":"Hepatology"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"41","DOI":"10.2147\/JHC.S61146","article-title":"Hepatocellular carcinoma: A review","volume":"3","author":"Balogh","year":"2016","journal-title":"J. Hepatocell Carcinoma"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1002\/hep.1840210308","article-title":"Incidence of hepatocellular carcinoma in chronic hepatitis B and C: A prospective study of 251 patients","volume":"21","author":"Takano","year":"1995","journal-title":"Hepatology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e247279","DOI":"10.1136\/bcr-2021-247279","article-title":"Delayed perihepatic abscess caused by Cutibacterium acnes following right partial hepatectomy","volume":"15","author":"Emeli","year":"2022","journal-title":"BMJ Case Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1007\/s12328-020-01283-5","article-title":"Liver abscess caused by Cutibacterium namnetense after transarterial chemoembolization for hepatocellular carcinoma","volume":"14","author":"Yasutomi","year":"2021","journal-title":"Clin. J. Gastroenterol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.1016\/j.transproceed.2016.01.077","article-title":"Profile of gut microbiota associated with the presence of hepatocellular cancer in patients with liver cirrhosis","volume":"48","author":"Grat","year":"2016","journal-title":"Transpl. Proc."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Cheong, H.S., Tan, S.P., Wong, D.M.K., Koo, W.L.Y., Wong, S.H., and Tan, N.S. (2023). The blood microbiome and health: Current evidence, controversies and challenges. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24065633"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Khalyfa, A.A., Punatar, S., and Yarbrough, A. (2022). Hepatocellular carcinoma: Understanding the inflammatory implications of the microbiome. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23158164"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2219","DOI":"10.15252\/embj.201490147","article-title":"The wound inflammatory response exacerbates growth of preneoplastic cells and progression to cancer","volume":"34","author":"Antonio","year":"2015","journal-title":"EMBO J."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/22\/3469\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:20:53Z","timestamp":1760131253000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/22\/3469"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,10]]},"references-count":37,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2023,11]]}},"alternative-id":["ani13223469"],"URL":"https:\/\/doi.org\/10.3390\/ani13223469","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,10]]}}}