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Emerging evidence indicates that ginsenoside Rg1 can effectively resist inflammation and pathological fibrosis in different organs. It is hypothesized that ginsenoside Rg1 may possess the potential to mitigate endometrial fibrosis induced by lipopolysaccharides (LPS) in dairy cows. Herein, a model of LPS-stimulated fibrosis was constructed using bovine endometrial epithelial cell line (BEND) cells and ICR mice. Western blotting was used to detect the protein level, and reactive oxygen species (ROS) content was measured by means of DCFH-DA. The uterine tissue structure was stained with H&amp;E and Masson staining. The murine endometrium was assessed for oxidative stress by detecting the concentration of MDA together with the activity of enzymatic antioxidants SOD and CAT. Ginsenoside Rg1 interfered with NLRP3 activation by reducing ROS generation. After the application of ROS inhibitor NAC and NLRP3 inhibitor MCC950, ginsenoside Rg1 could interfere in the ROS\/NLRP3 inflammasome signaling pathway by suppressing the epithelial\u2013mesenchymal transition (EMT) of BEND cells. Our in vivo data showed that ginsenoside Rg1 relieved endometrial fibrosis of the mouse model of LPS-induced endometritis by restraining the ROS\/NLRP3 inflammasome signaling pathway. Ginsenoside Rg1 inhibits LPS-induced EMT progression in BEND cells probably by inhibiting the activation of ROS-NLRP3 inflammasome.<\/jats:p>","DOI":"10.3390\/ani13233723","type":"journal-article","created":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T08:36:59Z","timestamp":1701419819000},"page":"3723","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Ginsenoside Rg1 Alleviates Lipopolysaccharide-Induced Fibrosis of Endometrial Epithelial Cells in Dairy Cows by Inhibiting Reactive Oxygen Species-Activated NLRP3"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1558-6708","authenticated-orcid":false,"given":"Liangli","family":"Song","sequence":"first","affiliation":[{"name":"College of Animal Science & Technology, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linnan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science & Technology, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangchen","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science & Technology, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5790-6645","authenticated-orcid":false,"given":"Longfei","family":"Xiao","sequence":"additional","affiliation":[{"name":"Veterinary Science (Traditional Chinese Medicine)\u2014Municipal Laboratory of Beijing, Beijing University of Agriculture, Beijing 102206, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1038\/s41586-020-2938-9","article-title":"Fibrosis: From mechanisms to medicines","volume":"587","author":"Henderson","year":"2020","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"883","DOI":"10.3389\/fimmu.2020.00883","article-title":"The Role of NLRP3 Inflammasome Activation in the Epithelial to Mesenchymal Transition Process During the Fibrosis","volume":"11","author":"Alyaseer","year":"2020","journal-title":"Front. Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1159\/000480321","article-title":"TGF-\u03b21 Induces EMT in Bovine Mammary Epithelial Cells Through the TGF\u03b21\/Smad Signaling Pathway","volume":"43","author":"Chen","year":"2017","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1089\/ars.2012.5149","article-title":"Reactive oxygen species in inflammation and tissue injury","volume":"20","author":"Mittal","year":"2014","journal-title":"Antioxid. Redox Signal."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"697643","DOI":"10.3389\/fphar.2021.697643","article-title":"Melatonin Alleviates Renal Injury in Mouse Model of Sepsis","volume":"12","author":"Chen","year":"2021","journal-title":"Front. Pharmacol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2620","DOI":"10.12659\/MSM.906362","article-title":"The Nephroprotective Effect of MS-275 on Lipopolysaccharide (LPS)-Induced Acute Kidney Injury by Inhibiting Reactive Oxygen Species (ROS)-Oxidative Stress and Endoplasmic Reticulum Stress","volume":"24","author":"Zhang","year":"2018","journal-title":"Med. Sci. Monit."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"13","DOI":"10.12703\/P6-13","article-title":"The M1 and M2 paradigm of macrophage activation: Time for reassessment","volume":"6","author":"Martinez","year":"2014","journal-title":"F1000Prime Rep."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Atri, C., Guerfali, F.Z., and Laouini, D. (2018). Role of Human Macrophage Polarization in Inflammation during Infectious Diseases. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19061801"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1016\/j.ecoenv.2018.07.076","article-title":"NLRP3 inflammasome activation and lung fibrosis caused by airborne fine particulate matter","volume":"163","author":"Zheng","year":"2018","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"801","DOI":"10.3892\/mmr.2021.12441","article-title":"Ginsenoside Rg1 ameliorates aging-induced liver fibrosis by inhibiting the NOX4\/NLRP3 inflammasome in SAMP8 mice","volume":"24","author":"Li","year":"2021","journal-title":"Mol. Med. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Zhang, D., Ji, P., Sun, R., Zhou, H., Huang, L., Kong, L., Li, W., and Li, W. (2022). Ginsenoside Rg1 attenuates LPS-induced chronic renal injury by inhibiting NOX4-NLRP3 signaling in mice. Biomed. Pharmacother., 150.","DOI":"10.1016\/j.biopha.2022.112936"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1816","DOI":"10.1111\/jcmm.13464","article-title":"H3 relaxin inhibits the collagen synthesis via ROS- and P2X7R-mediated NLRP3 inflammasome activation in cardiac fibroblasts under high glucose","volume":"22","author":"Zhang","year":"2018","journal-title":"J. Cell. Mol. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2538","DOI":"10.3389\/fimmu.2019.02538","article-title":"Pharmacological Inhibitors of the NLRP3 Inflammasome","volume":"10","author":"Zahid","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1111\/imr.12286","article-title":"Initiation and perpetuation of NLRP3 inflammasome activation and assembly","volume":"265","author":"Elliott","year":"2015","journal-title":"Immunol. Rev."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Liu, W., Han, X., Li, Q., Sun, L., and Wang, J. (2022). Iguratimod ameliorates bleomycin-induced pulmonary fibrosis by inhibiting the EMT process and NLRP3 inflammasome activation. Biomed. Pharmacother., 153.","DOI":"10.1016\/j.biopha.2022.113460"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1016\/j.jgr.2022.12.005","article-title":"Ginsenoside Rg1 Epigenetically Modulates Smad7 Expression in Liver Fibrosis via MicroRNA-152","volume":"47","author":"Zhang","year":"2023","journal-title":"J. Ginseng Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.jep.2008.11.020","article-title":"Ginsenoside Rg1, a major active component isolated from Panax notoginseng, restrains tubular epithelial to myofibroblast transition in vitro","volume":"122","author":"Xie","year":"2009","journal-title":"J. Ethnopharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/j.jgr.2021.03.006","article-title":"Ginsenoside Rg1 attenuates mechanical stress-induced cardiac injury via calcium sensing receptor-related pathway","volume":"45","author":"Lu","year":"2021","journal-title":"J. Ginseng Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1916","DOI":"10.3389\/fimmu.2018.01916","article-title":"The Potential Therapeutic Role of miR-223 in Bovine Endometritis by Targeting the NLRP3 Inflammasome","volume":"9","author":"Zhao","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Sisto, M., Ribatti, D., and Lisi, S. (2021). Organ Fibrosis and Autoimmunity: The Role of Inflammation in TGF\u03b2-Dependent EMT. Biomolecules, 11.","DOI":"10.3390\/biom11020310"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Shafique, L., Wu, S., Aqib, A.I., Ali, M.M., Ijaz, M., Naseer, M.A., Sarwar, Z., Ahmed, R., Saleem, A. (2021). Evidence-Based Tracking of MDR E. coli from Bovine Endometritis and Its Elimination by Effective Novel Therapeutics. Antibiotics, 10.","DOI":"10.3390\/antibiotics10080997"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Liu, Z., Pan, H., Zhang, Y., Zheng, Z., Xiao, W., Hong, X., Chen, F., Peng, X., Pei, Y., and Rong, J. (2022). Ginsenoside-Rg1 attenuates sepsis-induced cardiac dysfunction by modulating mitochondrial damage via the P2X7 receptor-mediated Akt\/GSK-3\u03b2 signaling pathway. J. Biochem. Mol. Toxicol., 36.","DOI":"10.1002\/jbt.22885"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"106339","DOI":"10.1016\/j.intimp.2020.106339","article-title":"Ginsenoside Rg1 ameliorates glomerular fibrosis during kidney aging by inhibiting NOX4 and NLRP3 inflammasome activation in SAMP8 mice","volume":"82","author":"Shen","year":"2020","journal-title":"Int. Immunopharmacol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/nm.3806","article-title":"A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases","volume":"21","author":"Coll","year":"2015","journal-title":"Nat. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1007\/s00395-022-00948-1","article-title":"Mitochondrial calpain-1 activates NLRP3 inflammasome by cleaving ATP5A1 and inducing mitochondrial ROS in CVB3-induced myocarditis","volume":"117","author":"Liu","year":"2022","journal-title":"Basic Res. Cardiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"F1081","DOI":"10.1152\/ajprenal.00534.2015","article-title":"NLRP3 deletion protects against renal fibrosis and attenuates mitochondrial abnormality in mouse with 5\/6 nephrectomy","volume":"310","author":"Gong","year":"2016","journal-title":"Am. J. Physiol. Renal. Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.1002\/smll.201402859","article-title":"NADPH Oxidase-Dependent NLRP3 Inflammasome Activation and its Important Role in Lung Fibrosis by Multiwalled Carbon Nanotubes","volume":"11","author":"Sun","year":"2015","journal-title":"Small"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.lfs.2019.02.035","article-title":"AIM2 gene silencing attenuates diabetic cardiomyopathy in type 2 diabetic rat model","volume":"221","author":"Wang","year":"2019","journal-title":"Life Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"115923","DOI":"10.1016\/j.jep.2022.115923","article-title":"Ginsenoside Rg1 attenuates glomerular fibrosis by inhibiting CD36\/TRPC6\/NFAT2 signaling in type 2 diabetes mellitus mice","volume":"302","author":"Han","year":"2023","journal-title":"J. Ethnopharmacol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1903627","DOI":"10.1155\/2020\/1903627","article-title":"Ginsenoside Rg1 Alleviates Podocyte EMT Passage by Regulating AKT\/GSK3 \u03b2\/\u03b2-Catenin Pathway by Restoring Autophagic Activity","volume":"2020","author":"Shi","year":"2020","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Mao, J., Ma, X., Zhu, J., and Zhang, H. (2022). Ginsenoside Rg1 ameliorates psoriasis-like skin lesions by suppressing proliferation and NLRP3 inflammasomes in keratinocytes. J. Food Biochem., 46.","DOI":"10.1111\/jfbc.14053"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/23\/3723\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:36:01Z","timestamp":1760132161000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/23\/3723"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,1]]},"references-count":31,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["ani13233723"],"URL":"https:\/\/doi.org\/10.3390\/ani13233723","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,1]]}}}