{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:18:33Z","timestamp":1777929513683,"version":"3.51.4"},"reference-count":47,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2023,12,2]],"date-time":"2023-12-02T00:00:00Z","timestamp":1701475200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study examined how separation behavior differs between dogs with and without separation-related problem behavior (SRB) and the possible risk factors. The study consisted of an online survey with 940 dog holders, which, in addition to demographic facts, also includes personality, emotional disposition and the attachment by the holder. Furthermore, a separation test was carried out with six non-SRB dogs over a maximum of 6 h, in which behavior and cortisol were determined. The questionnaire revealed that SRB dogs differed significantly from non-SRB dogs regarding the following factors: symptoms with at least a medium effect size such as restlessness, excitement, whining, howling, lip licking, barking and salivation, time to relax after separation, pessimism, persistence, excitability, calmness, separation frequency, greeting of holder and type of greeting. There were several other differences, but with weak effect sizes. The test showed that non-SRB dogs were mostly inactive during separation (lying resting and lying alert). Vocalization was almost non-existent. Behavior and cortisol did not change significantly over the different time periods. The data demonstrated typical symptoms and possible risk factors, some of which may be avoided or changed to improve animal welfare.<\/jats:p>","DOI":"10.3390\/ani13233735","type":"journal-article","created":{"date-parts":[[2023,12,2]],"date-time":"2023-12-02T13:45:41Z","timestamp":1701524741000},"page":"3735","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Factors Influencing Isolation Behavior of Dogs: A Holder-Based Questionnaire and Behavioral and Saliva Cortisol Responses during Separation"],"prefix":"10.3390","volume":"13","author":[{"given":"Jennifer","family":"Silbermann","sequence":"first","affiliation":[{"name":"Institute of Ecology and Evolution, Friedrich Schiller University Jena, Dornburger Str. 159, 07743 Jena, Germany"},{"name":"Institute of Zoology and Evolutionary Research with Phyletischem Museum, Ernst-Haeckel-Haus and Biodidactics, Friedrich Schiller University Jena, Erbertstr. 1, 07743 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Udo","family":"Ganslo\u00dfer","sequence":"additional","affiliation":[{"name":"Institute of Zoology and Evolutionary Research with Phyletischem Museum, Ernst-Haeckel-Haus and Biodidactics, Friedrich Schiller University Jena, Erbertstr. 1, 07743 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.applanim.2011.10.011","article-title":"The behavior of the domestic dog (Canis familiaris) during separation from and reunion with the holder: A questionnaire and an experimental study","volume":"135","author":"Konok","year":"2011","journal-title":"Appl. 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