{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:42:15Z","timestamp":1760150535822,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2023,12,5]],"date-time":"2023-12-05T00:00:00Z","timestamp":1701734400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The objectives were to determine the effects of dietary deoxynivalenol (DON) on apparent ileal digestibility (AID) of nutrients and to evaluate the efficacy of a bentonite (BEN) and a preservative blend (PB) product for alleviating DON effects on the nutrient digestibility of pigs. Twelve crossbred barrows with an initial body weight of 69.4 kg (standard deviation = 3.5) equipped with a T-cannula in the distal ileum were allotted a triplicated 4 \u00d7 2 incomplete Latin square design with four dietary treatments and two periods. Dietary treatments were (1) an uncontaminated diet, (2) a contaminated diet (CD) mainly based on contaminated wheat with 1.6 mg\/kg DON, (3) CD + 0.25% PB consisting of preservation components as major sources, antioxidants, microorganisms, and amino acids (AA), and (4) CD + 0.25% BEN. The AID and ATTD of dry matter, organic matter, crude protein, most minerals, and most AA were not affected by DON contamination. Dietary DON decreased the AID and ATTD of sodium (p &lt; 0.05) but were restored by supplementing the PB product (p &lt; 0.05). The AID of zinc was increased (p &lt; 0.05) by dietary DON, but supplementing BEN decreased zinc digestibility (p &lt; 0.05). The AID of Arg, Ile, Thr, and Asp was decreased (p &lt; 0.05) by BEN addition. In conclusion, dietary DON affected the digestibility of some minerals but not AA in pigs. Supplemental BEN can negatively affect the nutrient digestibility of some minerals and AA in pigs. The addition of a PB product in pig diets can restore digestibility of sodium but not of other nutrients. Based on these observations, feed additives for alleviating DON effects on nutrient digestibility of pigs can be carefully selected by swine diet formulators.<\/jats:p>","DOI":"10.3390\/ani13243752","type":"journal-article","created":{"date-parts":[[2023,12,5]],"date-time":"2023-12-05T08:09:17Z","timestamp":1701763757000},"page":"3752","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effects of a Bentonite Clay Product and a Preservative Blend on Ileal and Fecal Nutrient Digestibility in Pigs Fed Wheat Naturally Contaminated with Deoxynivalenol"],"prefix":"10.3390","volume":"13","author":[{"given":"Seung Youp","family":"Shin","sequence":"first","affiliation":[{"name":"Department of Animal Science, Konkuk University, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8331-2599","authenticated-orcid":false,"given":"Seung Bin","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Konkuk University, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoon Soo","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Konkuk University, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noa","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Konkuk University, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2097-717X","authenticated-orcid":false,"given":"Beob Gyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Konkuk University, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Chiba, L.I. (2023). Sustainable Swine Nutrition, Wiley Blackwell. [2nd ed.].","DOI":"10.1002\/9781119583998"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"243","DOI":"10.5713\/ajas.20.0137","article-title":"Effect of commercially purified deoxynivalenol and zearalenone mycotoxins on microbial diversity of pig cecum contents","volume":"34","author":"Reddy","year":"2021","journal-title":"Anim. Biosci."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Holanda, D.M., and Kim, S.W. (2021). Mycotoxin occurrence, toxicity, and detoxifying agents in pig production with an emphasis on deoxynivalenol. Toxins, 13.","DOI":"10.3390\/toxins13020171"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Kim, J., Jeong, J.Y., Sung, J.Y., and Kim, B.G. (2021). Equations to predict growth performance changes by dietary deoxynivalenol in pigs. Toxins, 13.","DOI":"10.3390\/toxins13050360"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"405","DOI":"10.5713\/ajas.20.0567","article-title":"Investigation of the efficacy of mycotoxin-detoxifying additive on health and growth of newly-weaned pigs under deoxynivalenol challenges","volume":"34","author":"Holanda","year":"2021","journal-title":"Anim. Biosci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1007\/s00204-017-2083-x","article-title":"Intestinal toxicity of deoxynivalenol is limited by Lactobacillus rhamnosus RC007 in pig jejunum explants","volume":"92","author":"Payros","year":"2018","journal-title":"Arch. Toxicol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.fct.2015.02.013","article-title":"Impact of deoxynivalenol (DON) contaminated feed on intestinal integrity and immune response in swine","volume":"80","author":"Lessard","year":"2015","journal-title":"Food Chem. Toxicol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.3390\/toxins5071261","article-title":"The use of feed additives to reduce the effects of aflatoxin and deoxynivalenol on pig growth, organ health and immune status during chronic exposure","volume":"5","author":"Weaver","year":"2013","journal-title":"Toxins"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Ahn, J.Y., Kim, J., Cheong, D.H., Hong, H., Jeong, J.Y., and Kim, B.G. (2022). An in vitro study on the efficacy of mycotoxin sequestering agents for aflatoxin B1, deoxynivalenol, and zearalenone. Animals, 12.","DOI":"10.3390\/ani12030333"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1186\/2193-1801-3-346","article-title":"Evaluation of mycotoxin sequestering agents for aflatoxin and deoxynivalenol: An in vitro approach","volume":"3","author":"Kong","year":"2014","journal-title":"Springerplus"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"197","DOI":"10.4141\/cjas-2014-126","article-title":"The efficacy of anti-mycotoxin feed additives in preventing the adverse effects of wheat naturally contaminated with Fusarium mycotoxins on performance, intestinal barrier function and nutrient digestibility and retention in weanling pigs","volume":"95","author":"Lessard","year":"2015","journal-title":"Can. J. Anim. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Kwon, W.B., Shin, S.Y., Song, Y.S., Kong, C., and Kim, B.G. (2023). Effects of mycotoxin-sequestering agents on growth performance and nutrient utilization of growing pigs fed deoxynivalenol-contaminated diets. Life, 13.","DOI":"10.3390\/life13101953"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"889612","DOI":"10.3389\/fvets.2022.889612","article-title":"Comprehensive review on the interactions of clay minerals with animal physiology and production","volume":"9","author":"Damato","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.anifeedsci.2013.04.010","article-title":"Effects of dietary supplementation of sericite on growth performance, nutrient digestibility, blood profiles and fecal microflora shedding in growing pigs","volume":"184","author":"Li","year":"2013","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.2527\/1993.7151209x","article-title":"Effects of feeding aflatoxin-contaminated diets with and without clay to weanling and growing pigs on performance, liver function, and mineral metabolism","volume":"71","author":"Schell","year":"1993","journal-title":"J. Anim. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"620","DOI":"10.2527\/jas.2013-6238","article-title":"Evaluation of two mycotoxin mitigation strategies in grow-finish swine diets containing corn dried distillers grains with solubles naturally contaminated with deoxynivalenol","volume":"92","author":"Patience","year":"2014","journal-title":"J. Anim. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.2527\/1998.7651433x","article-title":"Technical note: A technique for inserting a T-cannula into the distal ileum of pregnant sows","volume":"76","author":"Stein","year":"1998","journal-title":"J. Anim. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"591","DOI":"10.17533\/udea.rccp.324493","article-title":"A spreadsheet program for making a balanced Latin Square design","volume":"22","author":"Kim","year":"2009","journal-title":"Rev. Colomb. Cienc. Pecu."},{"key":"ref_19","unstructured":"NRC (2012). Nutrient Requirements of Swine."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.5713\/ajas.2014.14305","article-title":"Comparison of oven-drying methods for determination of moisture content in feed ingredients","volume":"27","author":"Ahn","year":"2014","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_21","unstructured":"AOAC (2019). Official Methods of Analysis, Association of Official Analytical Chemists International. [21st ed.]."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e20220155","DOI":"10.37496\/rbz5220220155","article-title":"Deoxynivalenol concentrations in feed ingredients and swine diets measured by enzyme- linked immunosorbent assay and high-performance liquid chromatography","volume":"52","author":"Ahn","year":"2023","journal-title":"Rev. Bras. Zootec."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"917","DOI":"10.5713\/ajas.2014.r.02","article-title":"Evaluation of amino Acid and energy utilization in feedstuff for swine and poultry diets","volume":"27","author":"Kong","year":"2014","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1080\/1745039X.2019.1641369","article-title":"Effects of deoxynivalenol and sodium meta-bisulphite on nutrient digestibility in growing pigs","volume":"73","author":"Bouchard","year":"2019","journal-title":"Arch. Anim. Nutr."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s12550-012-0139-6","article-title":"Inactivation of deoxynivalenol-contaminated cereal grains with sodium metabisulfite: A review of procedures and toxicological aspects","volume":"28","author":"Kersten","year":"2012","journal-title":"Mycotoxin Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/s12550-009-0030-2","article-title":"Effects of increasing concentrations of sodium metabisulphite (Na2S2O5, SBS) on deoxynivalenol (DON) concentration and microbial spoilage of triticale kernels preserved without and with propionic acid at various moisture contents","volume":"25","author":"Pahlow","year":"2009","journal-title":"Mycotoxin Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.anifeedsci.2016.06.006","article-title":"Effects of dietary deoxynivalenol and zearalenone on apparent ileal digestibility of amino acids in growing pigs","volume":"219","author":"Jo","year":"2016","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Holanda, D.M., Yiannikouris, A., and Kim, S.W. (2020). Investigation of the efficacy of a postbiotic yeast cell wall-based blend on newly-weaned pigs under a dietary challenge of multiple mycotoxins with emphasis on deoxynivalenol. Toxins, 12.","DOI":"10.3390\/toxins12080504"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1178","DOI":"10.2527\/jas1985.6151178x","article-title":"The effects of vomitoxin and feed intake on the performance and blood characteristics of young pigs","volume":"61","author":"Lun","year":"1985","journal-title":"J. Anim. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5713\/ajas.2014.r.01","article-title":"-Invited review\u2014Calcium digestibility and metabolism in pigs","volume":"27","author":"Stein","year":"2014","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"245","DOI":"10.5713\/ajas.17.0080","article-title":"Dietary maifanite supplementation did not affect the apparent total tract digestibility of calcium and phosphorus in growing pigs","volume":"31","author":"Bai","year":"2018","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1642","DOI":"10.5713\/ajas.2005.1642","article-title":"The effects of dietary Biotite V supplementation as an alternative substance to antibiotics in growing pigs","volume":"18","author":"Chen","year":"2005","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1111\/jpn.13724","article-title":"Dietary fumonisin may compromise the nutritive value of feed and distort copper and zinc digestibility and retention in weaned piglets","volume":"107","author":"Zeebone","year":"2023","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2838","DOI":"10.2527\/1992.7092838x","article-title":"Effects of hydrated sodium calcium aluminosilicate on fescue toxicosis and mineral absorption","volume":"70","author":"Chestnut","year":"1992","journal-title":"J. Anim. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"14308","DOI":"10.1021\/acs.est.9b04665","article-title":"Abiotic degradation of chlorinated solvents by clay minerals and Fe(II): Evidence for reactive mineral intermediates","volume":"53","author":"Entwistle","year":"2019","journal-title":"Environ. Sci. Technol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/0278-6915(91)90107-I","article-title":"Influence of subchronic exposure to low dietary deoxynivalenol, a trichothecene mycotoxin, on intestinal absorption of nutrients in mice","volume":"29","author":"Hunder","year":"1991","journal-title":"Food Chem. Toxicol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1080\/02652030701567467","article-title":"Reducing human exposure to aflatoxin through the use of clay: A review","volume":"25","author":"Phillips","year":"2008","journal-title":"Food Addit. Contam. A"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/s12550-013-0159-x","article-title":"Serum cation profile of broilers at various stages of exposure to deoxynivalenol","volume":"29","author":"Yunus","year":"2013","journal-title":"Mycotoxin Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"5713","DOI":"10.1021\/es204423k","article-title":"Zinc adsorption on clays inferred from atomistic simulations and EXAFS spectroscopy","volume":"46","author":"Churakov","year":"2012","journal-title":"Environ. Sci. Technol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2723","DOI":"10.1093\/jn\/132.9.2723","article-title":"The mycotoxin deoxynivalenol affects nutrient absorption in human intestinal epithelial cells","volume":"132","author":"Maresca","year":"2002","journal-title":"J. Nutr."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1956","DOI":"10.3945\/jn.110.123919","article-title":"Deoxynivalenol impairs porcine intestinal barrier function and decreases the protein expression of claudin-4 through a mitogen-activated protein kinase-dependent mechanism","volume":"140","author":"Pinton","year":"2010","journal-title":"J. Nutr."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1126\/science.424749","article-title":"L amino acids and D-glucose bind stereospecifically to a colloidal clay","volume":"203","author":"Bondy","year":"1979","journal-title":"Science"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"35332","DOI":"10.1039\/C4RA05216A","article-title":"Layered silicate clay functionalized with amino acids: Wound healing application","volume":"4","author":"Ghadiri","year":"2014","journal-title":"RSC Adv."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/24\/3752\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:33:34Z","timestamp":1760132014000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/24\/3752"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,5]]},"references-count":43,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["ani13243752"],"URL":"https:\/\/doi.org\/10.3390\/ani13243752","relation":{},"ISSN":["2076-2615"],"issn-type":[{"type":"electronic","value":"2076-2615"}],"subject":[],"published":{"date-parts":[[2023,12,5]]}}}