{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T11:35:35Z","timestamp":1778153735426,"version":"3.51.4"},"reference-count":50,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T00:00:00Z","timestamp":1701820800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Gansu Provincial Education Science and Technology Innovation Project","award":["GSSYLXM-02"],"award-info":[{"award-number":["GSSYLXM-02"]}]},{"name":"Gansu Provincial Education Science and Technology Innovation Project","award":["GSA-XMLZ-2021-01"],"award-info":[{"award-number":["GSA-XMLZ-2021-01"]}]},{"name":"Gansu Provincial Education Science and Technology Innovation Project","award":["GSSLCSX-2020-1"],"award-info":[{"award-number":["GSSLCSX-2020-1"]}]},{"name":"Gansu Provincial Beef Cattle Quality Fattening Project","award":["GSSYLXM-02"],"award-info":[{"award-number":["GSSYLXM-02"]}]},{"name":"Gansu Provincial Beef Cattle Quality Fattening Project","award":["GSA-XMLZ-2021-01"],"award-info":[{"award-number":["GSA-XMLZ-2021-01"]}]},{"name":"Gansu Provincial Beef Cattle Quality Fattening Project","award":["GSSLCSX-2020-1"],"award-info":[{"award-number":["GSSLCSX-2020-1"]}]},{"name":"Gansu Provincial Agricultural Special Project","award":["GSSYLXM-02"],"award-info":[{"award-number":["GSSYLXM-02"]}]},{"name":"Gansu Provincial Agricultural Special Project","award":["GSA-XMLZ-2021-01"],"award-info":[{"award-number":["GSA-XMLZ-2021-01"]}]},{"name":"Gansu Provincial Agricultural Special Project","award":["GSSLCSX-2020-1"],"award-info":[{"award-number":["GSSLCSX-2020-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The aim of this study was to investigate the effect of oregano essential oil on IgA+, IgG+, and IgM+ cells in the jejunum of castrated Holstein bulls. Twelve castrated Holstein bulls were randomly divided into control (YCK) and oregano essential oil (YEO) groups. Pathological changes in the jejunum were observed by HE staining, and the expression levels of IgA, IgG, and IgM in the jejunum were detected by ELISA. The distributions of IgA+, IgG+, and IgM+ cells in the jejunum were analysed by multiplex immunofluorescence and immunohistochemistry. The results showed that the jejunal villi were detached in the YCK group, which may have been related to inflammation, while the intestinal epithelium was clear and intact in the YEO group. The expressions of IgA, IgG, and IgM were significantly reduced by 40.75%, 30.76%, and 50.87%. The IgA+, IgG+, and IgM+ cells were diffusely distributed in the lamina propria of the jejunum, and were reduced by 17.07%, 6.44%, and 6.15%, respectively. Oregano essential oil did not alter the distribution characteristics of IgA+, IgG+, or IgM+ cells in the jejunum, but it suppressed inflammatory response, decreased immunoglobulin content, and significantly enhanced the formation of an immune barrier in the gastrointestinal mucosa.<\/jats:p>","DOI":"10.3390\/ani13243766","type":"journal-article","created":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T08:48:32Z","timestamp":1701852512000},"page":"3766","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effects of Oregano Essential Oil on IgA+, IgG+, and IgM+ Cells in the Jejunum of Castrated Holstein Bulls"],"prefix":"10.3390","volume":"13","author":[{"given":"Qiyan","family":"Liu","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wangdong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Medicine, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baoshan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Medicine, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinping","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengjia","family":"He","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Jia","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3651-9501","authenticated-orcid":false,"given":"Yongliang","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meiling","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Cheng","sequence":"additional","affiliation":[{"name":"Jing Chuan Xu Kang Food Co., Ltd., Pingliang 745000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaomin","family":"Lei","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/nri3608","article-title":"Intestinal epithelial cells: Regulators of barrier function and immune homeostasis","volume":"14","author":"Peterson","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1126\/science.aaq0926","article-title":"Gut microbiota utilize immunoglobulin a for mucosal colonization","volume":"360","author":"Donaldson","year":"2018","journal-title":"Science"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/978-981-15-3532-1_9","article-title":"Diversified IgA-bacteria interaction in gut homeostasis","volume":"1254","author":"Suzuki","year":"2020","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"728853","DOI":"10.3389\/fimmu.2021.728853","article-title":"A diagnostic model with IgM autoantibodies and carcinoembryonic antigen for early detection of lung adenocarcinoma","volume":"12","author":"Zhang","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2461","DOI":"10.1016\/j.immuni.2023.10.014","article-title":"Dimeric IgA specifically disables intracellular mutated oncodrivers","volume":"56","author":"Prince","year":"2023","journal-title":"Immunity"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1111\/imr.12980","article-title":"Fantastic IgA plasma cells and where to find them","volume":"303","author":"Isho","year":"2021","journal-title":"Immunol. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"l3927","DOI":"10.1126\/scitranslmed.abl3927","article-title":"The systemic anti-microbiota igg repertoire can identify gut bacteria that translocate across gut barrier surfaces","volume":"14","author":"Welles","year":"2022","journal-title":"Sci. Transl. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"h3150","DOI":"10.1126\/sciadv.adh3150","article-title":"Characterization of the antispike IgG immune response to COVID-19 vaccines in people with a wide variety of immunodeficiencies","volume":"9","author":"Zendt","year":"2023","journal-title":"Sci. Adv."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"107527","DOI":"10.1016\/j.isci.2023.107527","article-title":"Serum concentration of antigen-specific igg can substantially bias interpretation of antibody-dependent phagocytosis assay readout","volume":"26","author":"St","year":"2023","journal-title":"iScience"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"16","DOI":"10.4049\/jimmunol.2101196","article-title":"At critically low antigen densities, IgM hexamers outcompete both IgM pentamers and igg1 for human complement deposition and complement-dependent cytotoxicity","volume":"209","author":"Oskam","year":"2022","journal-title":"J. Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1111\/imm.13629","article-title":"Dual blockages of a broad and potent neutralizing IgM antibody targeting GH loop of EV-As","volume":"169","author":"Zhu","year":"2023","journal-title":"Immunology"},{"key":"ref_12","first-page":"1719","article-title":"Composition of the essential oil of Origanum tyttanthum from Tajikistan","volume":"6","author":"Sharopov","year":"2011","journal-title":"Nat. Prod. Commun."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Cheng, C., Xia, M., Zhang, X., Wang, C., Jiang, S., and Peng, J. (2018). Supplementing oregano essential oil in a reduced-protein diet improves growth performance and nutrient digestibility by modulating intestinal bacteria, intestinal morphology, and antioxidative capacity of growing-finishing pigs. Animals, 8.","DOI":"10.3390\/ani8090159"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Tan, C., Wei, H., Sun, H., Ao, J., Long, G., Jiang, S., and Peng, J. (2015). Effects of dietary supplementation of oregano essential oil to sows on oxidative stress status, lactation feed intake of sows, and piglet performance. Biomed. Res. Int., 2015.","DOI":"10.1155\/2015\/525218"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Hall, H.N., Wilkinson, D.J., and Le Bon, M. (2021). Oregano essential oil improves piglet health and performance through maternal feeding and is associated with changes in the gut microbiota. Anim. Microbiome, 3.","DOI":"10.1186\/s42523-020-00064-2"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Zhou, R., Wu, J., Zhang, L., Liu, L., Casper, D.P., Jiao, T., Liu, T., Wang, J., Lang, X., and Song, S. (2019). Effects of oregano essential oil on the ruminal ph and microbial population of sheep. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0217054"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"101242","DOI":"10.1016\/j.psj.2021.101242","article-title":"Effects of essential oil on growth performance, digestibility, immunity, and intestinal health in broilers","volume":"100","author":"Su","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"101163","DOI":"10.1016\/j.psj.2021.101163","article-title":"Effects of oregano essential oil as an antibiotic growth promoter alternative on growth performance, antioxidant status, and intestinal health of broilers","volume":"100","author":"Zhang","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"skab033","DOI":"10.1093\/jas\/skab033","article-title":"Effects of dietary oregano essential oil supplementation on growth performance, intestinal antioxidative capacity, immunity, and intestinal microbiota in yellow-feathered chickens","volume":"99","author":"Ruan","year":"2021","journal-title":"J. Anim. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"722557","DOI":"10.3389\/fnut.2021.722557","article-title":"Oregano essential oils promote rumen digestive ability by modulating epithelial development and microbiota composition in beef cattle","volume":"8","author":"Zhang","year":"2021","journal-title":"Front. Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"109317","DOI":"10.1016\/j.meatsci.2023.109317","article-title":"Dietary oregano essential oil supplementation alters meat quality, oxidative stability, and fatty acid profiles of beef cattle","volume":"205","author":"He","year":"2023","journal-title":"Meat Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"11634","DOI":"10.1038\/s41598-018-29897-3","article-title":"Dietary supplementation with essential-oils-cobalt for improving growth performance, meat quality and skin cell capacity of goats","volume":"8","author":"Lei","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1101\/pdb.prot073411","article-title":"Manual hematoxylin and eosin staining of mouse tissue sections","volume":"2014","author":"Cardiff","year":"2014","journal-title":"Cold Spring Harb. Protoc."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"878467","DOI":"10.3389\/fvets.2022.878467","article-title":"Moniezia benedeni infection restrain IgA+, IgG+, and IgM+ cells residence in sheep (Ovis aries) small intestine","volume":"9","author":"Han","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Chen, M., Xie, W., Zhou, S., Ma, N., Wang, Y., Huang, J., Shen, X., and Chang, G. (2023). A high-concentrate diet induces colonic inflammation and barrier damage in hu sheep. J. Dairy Sci., in press.","DOI":"10.3168\/jds.2023-23359"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"s399","DOI":"10.1017\/S1751731118001921","article-title":"Review: Enhancing gastrointestinal health in dairy cows","volume":"12","author":"Plaizier","year":"2018","journal-title":"Animal"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"R1288","DOI":"10.1152\/ajpregu.00494.2015","article-title":"Twelve hours of heat stress induces inflammatory signaling in porcine skeletal muscle","volume":"310","author":"Ganesan","year":"2016","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1080\/03079457.2012.709315","article-title":"Heat stress impairs performance and induces intestinal inflammation in broiler chickens infected with salmonella enteritidis","volume":"41","author":"Gomes","year":"2012","journal-title":"Avian Pathol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1986","DOI":"10.2527\/jas.2011-3949","article-title":"Acute heat stress impairs performance parameters and induces mild intestinal enteritis in broiler chickens: Role of acute hypothalamic-pituitary-adrenal axis activation","volume":"90","author":"Rodrigues","year":"2012","journal-title":"J. Anim. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Monson, M.S., Van Goor, A.G., Ashwell, C.M., Persia, M.E., Rothschild, M.F., Schmidt, C.J., and Lamont, S.J. (2018). Immunomodulatory effects of heat stress and lipopolysaccharide on the bursal transcriptome in two distinct chicken lines. BMC Genom., 19.","DOI":"10.1186\/s12864-018-5033-y"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5432","DOI":"10.3382\/ps\/pez327","article-title":"Heat shock protein 70 protects the quail cecum against oxidant stress, inflammatory injury, and microbiota imbalance induced by cold stress","volume":"98","author":"Liu","year":"2019","journal-title":"Poultry Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1038\/ismej.2011.212","article-title":"The human small intestinal microbiota is driven by rapid uptake and conversion of simple carbohydrates","volume":"6","author":"Zoetendal","year":"2012","journal-title":"ISME J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"45840","DOI":"10.1038\/srep45840","article-title":"Microbial biogeography and core microbiota of the rat digestive tract","volume":"8","author":"Li","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"137r","DOI":"10.1126\/scitranslmed.3004184","article-title":"Microbiota, disease, and back to health: A metastable journey","volume":"4","author":"Blumberg","year":"2012","journal-title":"Sci. Transl. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.tibtech.2015.06.011","article-title":"Proteobacteria: Microbial signature of dysbiosis in gut microbiota","volume":"33","author":"Shin","year":"2015","journal-title":"Trends Biotechnol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"803691","DOI":"10.3389\/fnut.2021.803691","article-title":"Lentinan supplementation protects the gut\u2013liver axis and prevents steatohepatitis: The role of gut microbiota involved","volume":"8","author":"Yang","year":"2022","journal-title":"Front. Nutr."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.alit.2018.09.004","article-title":"A comprehensive understanding of the gut mucosal immune system in allergic inflammation","volume":"68","author":"Tokuhara","year":"2019","journal-title":"Allergol. Int."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"423","DOI":"10.2183\/pjab.92.423","article-title":"Maintenance of gut homeostasis by the mucosal immune system","volume":"92","author":"Okumura","year":"2016","journal-title":"Proc. Jpn. Acad. Ser. B Phys. Biol. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"19322","DOI":"10.1038\/s41598-023-46421-4","article-title":"Comprehensive antibody and cytokine profiling in hospitalized covid-19 patients in relation to clinical outcomes in a large belgian cohort","volume":"13","author":"Ruytinx","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e2985","DOI":"10.1093\/cid\/ciaa1850","article-title":"Symptomatic severe acute respiratory syndrome coronavirus 2 reinfection of a healthcare worker in a Belgian nosocomial outbreak despite primary neutralizing antibody response","volume":"73","author":"Selhorst","year":"2021","journal-title":"Clin. Infect. Dis."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1879","DOI":"10.1038\/s41590-023-01637-4","article-title":"Fungal microbiota sustains lasting immune activation of neutrophils and their progenitors in severe COVID-19","volume":"24","author":"Takato","year":"2023","journal-title":"Nat. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1253\/circj.CJ-14-0471","article-title":"Omega 3 polyunsaturated fatty acids suppress the development of aortic aneurysms through the inhibition of macrophage-mediated inflammation","volume":"79","author":"Yoshihara","year":"2015","journal-title":"Circ. J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"39211","DOI":"10.1038\/srep39211","article-title":"Epoxygenated fatty acids inhibit retinal vascular inflammation","volume":"6","author":"Capozzi","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"G177","DOI":"10.1152\/ajpgi.00307.2002","article-title":"Amelioration of dextran sulfate colitis by butyrate: Role of heat shock protein 70 and NF-kappab","volume":"285","author":"Venkatraman","year":"2003","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1038\/mi.2016.93","article-title":"Eosinophils are required to suppress Th2 responses in Peyer\u2019s patches during intestinal infection by nematodes","volume":"10","author":"Strandmark","year":"2017","journal-title":"Mucosal Immunol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1038\/s41385-019-0202-0","article-title":"Class-switch recombination to IgA in the Peyer\u2019s patches requires natural thymus-derived Tregs and appears to be antigen independent","volume":"12","author":"Gribonika","year":"2019","journal-title":"Mucosal Immunol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Lee, J., Chou, N., Yu, Y., Shih, H., Lin, H., Lee, C., and Chang, M. (2023). Phrf1 promotes the class switch recombination of IgA in CH12F3-2A cells. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0285159"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.imlet.2017.04.016","article-title":"Cytokine profile of NALT during acute stress and its possible effect on IgA secretion","volume":"188","year":"2017","journal-title":"Immunol. Lett."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s10067-018-4234-8","article-title":"Insight into inflammatory cell and cytokine profiles in adult IgA vasculitis","volume":"38","author":"Kuret","year":"2019","journal-title":"Clin. Rheumatol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1684\/ecn.2019.0434","article-title":"Il-10 induces tgf-\u03b2secretion, tgf-\u03b2receptor ii upregulation, and iga secretion in b cells","volume":"30","author":"Bagheri","year":"2019","journal-title":"Eur. Cytokine Netw."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/24\/3766\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:34:13Z","timestamp":1760132053000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/13\/24\/3766"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,6]]},"references-count":50,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["ani13243766"],"URL":"https:\/\/doi.org\/10.3390\/ani13243766","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,6]]}}}