{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:39:26Z","timestamp":1787240366808,"version":"build-2736575974"},"reference-count":59,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T00:00:00Z","timestamp":1702598400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["2021CFB559"],"award-info":[{"award-number":["2021CFB559"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["2023AFA018"],"award-info":[{"award-number":["2023AFA018"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["T2022024"],"award-info":[{"award-number":["T2022024"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Foundation for Innovative Research Groups of Hubei Provincial Natural Science Foundation","award":["2021CFB559"],"award-info":[{"award-number":["2021CFB559"]}]},{"name":"Foundation for Innovative Research Groups of Hubei Provincial Natural Science Foundation","award":["2023AFA018"],"award-info":[{"award-number":["2023AFA018"]}]},{"name":"Foundation for Innovative Research Groups of Hubei Provincial Natural Science Foundation","award":["T2022024"],"award-info":[{"award-number":["T2022024"]}]},{"name":"Hubei Provincial Department of Education","award":["2021CFB559"],"award-info":[{"award-number":["2021CFB559"]}]},{"name":"Hubei Provincial Department of Education","award":["2023AFA018"],"award-info":[{"award-number":["2023AFA018"]}]},{"name":"Hubei Provincial Department of Education","award":["T2022024"],"award-info":[{"award-number":["T2022024"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study was conducted to investigate effects of dietary Limosilactobacillus fermentum and Lacticaseibacillus paracasei supplementation on the intestinal stem cell proliferation, immunity, and ileal microbiota of broiler chickens challenged by coccidia and Clostridium perfringens. A total of 336 one-day-old Ross 308 chickens were randomly assigned into four groups. Chickens in the control (CTR) group were fed basal diet, and chickens in the three challenged groups were fed basal diets supplemented with nothing (CCP group), 1.0 \u00d7 109 CFU\/kg L. fermentum (LF_CCP group), and 1.0 \u00d7 109 CFU\/kg L. paracasei (LP_CCP group), respectively. All challenged birds were infected with coccildia on day 9 and Clostridium perfringens during days 13\u201318. The serum and intestinal samples were collected on days 13 and 19. The results showed that L. fermentum significantly increased jejunal gene expression of cdxB (one of the intestinal stem cell marker genes) on day 13. Additionally, L. fermentum significantly up-regulated mRNA levels of JAK3 and TYK2 and tended to increase STAT6 mRNA expression in jejunum on day 19. In the cecal tonsil, both L. fermentum and L. paracasei decreased mRNA expression of JAK2 on day 13, and L. fermentum down-regulated JAK1-2, STAT1, and STAT5-6 gene expressions on day 19. Ileal microbiological analysis showed that coccidial infection increased the Escherichia\u2013Shigella, Lactobacillus, and Romboutsia abundance and decreased Candidatus_Arthromitus richness on day 13, which were reversed by Lactobacillus intervention. Moreover, Lactobacilli increased ileal Lactobacillus richness on day 19. In conclusion, Lactobacilli alleviated the impairment of intestinal stem cell proliferation and immunity in coccidia- and C. perfringens-challenged birds via modulating JAK\/STAT signaling and reshaping intestinal microflora.<\/jats:p>","DOI":"10.3390\/ani13243864","type":"journal-article","created":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T08:12:57Z","timestamp":1702627977000},"page":"3864","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Effects of Dietary Limosilactobacillus fermentum and Lacticaseibacillus paracasei Supplementation on the Intestinal Stem Cell Proliferation, Immunity, and Ileal Microbiota of Broiler Chickens Challenged by Coccidia and Clostridium perfringens"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4852-5124","authenticated-orcid":false,"given":"Shuangshuang","family":"Guo","sequence":"first","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural by-Products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenfei","family":"Tong","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural by-Products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Qi","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural by-Products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meihan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural by-Products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Li","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural by-Products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengfan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural by-Products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qunbing","family":"Hu","sequence":"additional","affiliation":[{"name":"Hubei Horwath Biotechnology Co., Ltd., Xianning 437099, China"},{"name":"Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Sciences, Hunan Normal University, Changsha 410081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuan","family":"Song","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural by-Products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binying","family":"Ding","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural by-Products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"101590","DOI":"10.1016\/j.psj.2021.101590","article-title":"Necrotic enteritis in broiler chickens: Disease characteristics and prevention using organic antibiotic alternatives\u2014A comprehensive review","volume":"101","author":"Elbestawy","year":"2022","journal-title":"Poult. 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