{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T19:18:05Z","timestamp":1789586285779,"version":"build-2803163510"},"reference-count":39,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,12,22]],"date-time":"2023-12-22T00:00:00Z","timestamp":1703203200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Israeli Dairy Board","award":["983-0035-18"],"award-info":[{"award-number":["983-0035-18"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>In Israel, about 26% of produced milk is used to produce hard cheeses and 29% for soft cheeses. Milk with preferred coagulation properties requires a shorter coagulation time and yields a higher curd firmness than milk with inferior coagulation properties. Studies have shown that milk from cows with the B allele of kappa casein (\u03ba-CN) produces more cheese than milk from those with A and E alleles. There is evidence that milk from AE or EE genotype cows is unsuitable for cheese production. In the early 1990s, the proportion of the B allele in Israeli Holstein cattle was about 17%, similar to its prevalence in the Holstein population worldwide. In recent years, however, its proportion has increased to about 40%. We analyzed milk coagulation properties as a function of the cow\u2019s \u03ba-CN genotype, including time in minutes until the beginning of coagulation and curd firmness after 60 min\u2014measured in volts via an optigraph device and scored on a scale of 0\u20134 by a laboratory technician. Cow selection was based on their sire\u2019s genotype, so that there would be sufficient genotypes that include the rare E allele. A total of 359 cows were sampled from 15 farms: 64 with genotype AA, 142 with AB, 41 with AE, 65 with BB, and 47 with BE. Data were analyzed via the general linear model procedure of SAS. We found the following: (a) There were significant differences between genotypes for optigraph-measured curd firmness. In a multi-comparison test, the BB genotype gave the highest curd firmness, and AB and BE showed a significant advantage compared to AA and AE (9.4, 8.6, 8.4, 6.9, 6.8 V, respectively). Assuming a frequency of about 55% for the A allele, about 30% of the milk delivered to dairy plants comes from AA cows. (b) There was a significant difference between the genotypes in technician-observed curd firmness, with BB scoring significantly higher than AA and AE. (c) The optigraph-measured curd firmness was significantly higher for milk from primiparous cows as compared to milk from second, third, or fourth lactation cows (8.9, 7.8, 7.9, 7.7 V, respectively). The technician-observed curd firmness was significantly higher for primiparous vs. multiparous cows. There was a clear advantage in curd firmness for genotypes that included the B allele compared to those with AA and AE genotypes. We can increase the proportion of the B allele in the population by insemination of cows using bulls with the genotypes AB and BB. This factor should therefore be included in the selection index.<\/jats:p>","DOI":"10.3390\/ani14010054","type":"journal-article","created":{"date-parts":[[2023,12,22]],"date-time":"2023-12-22T08:53:01Z","timestamp":1703235181000},"page":"54","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Estimating the Effect of the Kappa Casein Genotype on Milk Coagulation Properties in Israeli Holstein Cows"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3371-2904","authenticated-orcid":false,"given":"Yaniv","family":"Lavon","sequence":"first","affiliation":[{"name":"Israel Cattle Breeders Association, Caesaria Industrial Park, Caesarea 38900, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0668-1594","authenticated-orcid":false,"given":"Joel I.","family":"Weller","sequence":"additional","affiliation":[{"name":"Israel Cattle Breeders Association, Caesaria Industrial Park, Caesarea 38900, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoel","family":"Zeron","sequence":"additional","affiliation":[{"name":"Sion Artificial Insemination Center, Gadara 7057102, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ephraim","family":"Ezra","sequence":"additional","affiliation":[{"name":"Israel Cattle Breeders Association, Caesaria Industrial Park, Caesarea 38900, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6483","DOI":"10.3168\/jds.2012-5507","article-title":"Genetics and modeling of milk coagulation properties","volume":"95","author":"Bittante","year":"2012","journal-title":"J. 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