{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T06:44:22Z","timestamp":1787985862680,"version":"build-2784847793"},"reference-count":97,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,12,26]],"date-time":"2023-12-26T00:00:00Z","timestamp":1703548800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Program of China","award":["2022YFD2400100"],"award-info":[{"award-number":["2022YFD2400100"]}]},{"name":"National Key R&amp;D Program of China","award":["31722058"],"award-info":[{"award-number":["31722058"]}]},{"name":"National Key R&amp;D Program of China","award":["32002371"],"award-info":[{"award-number":["32002371"]}]},{"name":"National Key R&amp;D Program of China","award":["2023TD19"],"award-info":[{"award-number":["2023TD19"]}]},{"name":"National Key R&amp;D Program of China","award":["CARS-47-G03"],"award-info":[{"award-number":["CARS-47-G03"]}]},{"name":"National Nature Science Foundation of China","award":["2022YFD2400100"],"award-info":[{"award-number":["2022YFD2400100"]}]},{"name":"National Nature Science Foundation of China","award":["31722058"],"award-info":[{"award-number":["31722058"]}]},{"name":"National Nature Science Foundation of China","award":["32002371"],"award-info":[{"award-number":["32002371"]}]},{"name":"National Nature Science Foundation of China","award":["2023TD19"],"award-info":[{"award-number":["2023TD19"]}]},{"name":"National Nature Science Foundation of China","award":["CARS-47-G03"],"award-info":[{"award-number":["CARS-47-G03"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["2022YFD2400100"],"award-info":[{"award-number":["2022YFD2400100"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["31722058"],"award-info":[{"award-number":["31722058"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["32002371"],"award-info":[{"award-number":["32002371"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["2023TD19"],"award-info":[{"award-number":["2023TD19"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["CARS-47-G03"],"award-info":[{"award-number":["CARS-47-G03"]}]},{"name":"China Agriculture Research System","award":["2022YFD2400100"],"award-info":[{"award-number":["2022YFD2400100"]}]},{"name":"China Agriculture Research System","award":["31722058"],"award-info":[{"award-number":["31722058"]}]},{"name":"China Agriculture Research System","award":["32002371"],"award-info":[{"award-number":["32002371"]}]},{"name":"China Agriculture Research System","award":["2023TD19"],"award-info":[{"award-number":["2023TD19"]}]},{"name":"China Agriculture Research System","award":["CARS-47-G03"],"award-info":[{"award-number":["CARS-47-G03"]}]},{"name":"National Marine Genetic Resource Center","award":["2022YFD2400100"],"award-info":[{"award-number":["2022YFD2400100"]}]},{"name":"National Marine Genetic Resource Center","award":["31722058"],"award-info":[{"award-number":["31722058"]}]},{"name":"National Marine Genetic Resource Center","award":["32002371"],"award-info":[{"award-number":["32002371"]}]},{"name":"National Marine Genetic Resource Center","award":["2023TD19"],"award-info":[{"award-number":["2023TD19"]}]},{"name":"National Marine Genetic Resource Center","award":["CARS-47-G03"],"award-info":[{"award-number":["CARS-47-G03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>As a common influencing factor in the environment, temperature greatly influences the fish that live in the water all their life. The essential economic fish Chinese tongue sole (Cynoglossus semilaevis), a benthic fish, will experience both physiological and behavioral changes due to increases in temperature. The brain, as the central hub of fish and a crucial regulatory organ, is particularly sensitive to temperature changes and will be affected. However, previous research has mainly concentrated on the impact of temperature on the gonads of C. semilaevis. Instead, our study examines the brain using transcriptomics to investigate specific genes and pathways that can quickly respond to temperature changes. The fish were subjected to various periods of heat stress (1 h, 2 h, 3 h, and 5 h) before extracting the brain for transcriptome analysis. After conducting transcriptomic analyses, we identified distinct genes and pathways in males and females. The pathways were mainly related to cortisol synthesis and secretion, neuroactive ligand\u2013receptor interactions, TGF beta signaling pathway, and JAK\/STAT signaling pathway, while the genes included the HSP family, tshr, c-fos, c-jun, cxcr4, camk2b, and igf2. Our study offers valuable insights into the regulation mechanisms of the brain\u2019s response to temperature stress.<\/jats:p>","DOI":"10.3390\/ani14010084","type":"journal-article","created":{"date-parts":[[2023,12,25]],"date-time":"2023-12-25T23:00:12Z","timestamp":1703545212000},"page":"84","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Transcriptome Analysis Revealed the Early Heat Stress Response in the Brain of Chinese Tongue Sole (Cynoglossus semilaevis)"],"prefix":"10.3390","volume":"14","author":[{"given":"Yue","family":"Wang","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Fisheries College, Tianjin Agricultural University, Tianjin 300384, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengcheng","family":"Su","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Liu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiancai","family":"Hao","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenglei","family":"Han","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas B.","family":"Doretto","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6128-0099","authenticated-orcid":false,"given":"Ivana F.","family":"Rosa","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology, Institute of Biosciences, S\u00e3o Paulo State University (UNESP), Botucatu 01049-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjing","family":"Yang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Fisheries College, Tianjin Agricultural University, Tianjin 300384, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6953-2203","authenticated-orcid":false,"given":"Changwei","family":"Shao","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao 266237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0326-4410","authenticated-orcid":false,"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao 266237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1152\/physrev.1997.77.3.591","article-title":"The stress response in fish","volume":"77","year":"1997","journal-title":"Physiol. 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