{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T15:56:19Z","timestamp":1773158179968,"version":"3.50.1"},"reference-count":75,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T00:00:00Z","timestamp":1706054400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Key R&amp;D Program of China","award":["2023YFD2402000"],"award-info":[{"award-number":["2023YFD2402000"]}]},{"name":"the National Key R&amp;D Program of China","award":["2023ZDZX4009"],"award-info":[{"award-number":["2023ZDZX4009"]}]},{"name":"the National Key R&amp;D Program of China","award":["32072988"],"award-info":[{"award-number":["32072988"]}]},{"name":"the National Key R&amp;D Program of China","award":["2022A1515012325"],"award-info":[{"award-number":["2022A1515012325"]}]},{"name":"the National Key R&amp;D Program of China","award":["2022A1515011943"],"award-info":[{"award-number":["2022A1515011943"]}]},{"name":"Special Projects in Key Fields of Ordinary University in Guangdong Province","award":["2023YFD2402000"],"award-info":[{"award-number":["2023YFD2402000"]}]},{"name":"Special Projects in Key Fields of Ordinary University in Guangdong Province","award":["2023ZDZX4009"],"award-info":[{"award-number":["2023ZDZX4009"]}]},{"name":"Special Projects in Key Fields of Ordinary University in Guangdong Province","award":["32072988"],"award-info":[{"award-number":["32072988"]}]},{"name":"Special Projects in Key Fields of Ordinary University in Guangdong Province","award":["2022A1515012325"],"award-info":[{"award-number":["2022A1515012325"]}]},{"name":"Special Projects in Key Fields of Ordinary University in Guangdong Province","award":["2022A1515011943"],"award-info":[{"award-number":["2022A1515011943"]}]},{"name":"the National Natural Science Foundation of China","award":["2023YFD2402000"],"award-info":[{"award-number":["2023YFD2402000"]}]},{"name":"the National Natural Science Foundation of China","award":["2023ZDZX4009"],"award-info":[{"award-number":["2023ZDZX4009"]}]},{"name":"the National Natural Science Foundation of China","award":["32072988"],"award-info":[{"award-number":["32072988"]}]},{"name":"the National Natural Science Foundation of China","award":["2022A1515012325"],"award-info":[{"award-number":["2022A1515012325"]}]},{"name":"the National Natural Science Foundation of China","award":["2022A1515011943"],"award-info":[{"award-number":["2022A1515011943"]}]},{"name":"General Program of Natural Science Foundation of Guangdong Province, China","award":["2023YFD2402000"],"award-info":[{"award-number":["2023YFD2402000"]}]},{"name":"General Program of Natural Science Foundation of Guangdong Province, China","award":["2023ZDZX4009"],"award-info":[{"award-number":["2023ZDZX4009"]}]},{"name":"General Program of Natural Science Foundation of Guangdong Province, China","award":["32072988"],"award-info":[{"award-number":["32072988"]}]},{"name":"General Program of Natural Science Foundation of Guangdong Province, China","award":["2022A1515012325"],"award-info":[{"award-number":["2022A1515012325"]}]},{"name":"General Program of Natural Science Foundation of Guangdong Province, China","award":["2022A1515011943"],"award-info":[{"award-number":["2022A1515011943"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study explored the effects of dietary protein levels on Litopenaeus vannamei with its intestinal microbiota and transcriptome responses. Previous studies on the effects of dietary protein levels on L. vannamei have focused on growth performance, antioxidant indices, and digestive enzyme activity, but few studies have been conducted at the microbiological and molecular levels. In this study, five isolipid experimental diets with protein levels of 32% (P32), 36% (P36), 40% (P40), 44% (P44), and 48% (P48) were used in an L. vannamei (0.63 \u00b1 0.02 g) feeding trial for 56 days. At the end of the feeding trial, the growth performance, immunity, intestinal health, and transcriptional responses of L. vannamei were determined. This study demonstrated that higher protein levels (P44) led to superior weight gain and growth rates for L. vannamei, with lower feed conversion ratios (FCR) observed in the P48 and P44 groups compared to the P32 and P36 groups (p \u2264 0.05). The P44 and P48 groups also showed a notably higher protein efficiency ratio (PER) compared to others (p \u2264 0.05), and there was no significant difference between them. Upon Vibrio parahaemolyticus infection, the P48 group exhibited a significantly lower survival rate (SR) within 48 h, while during 72 h of white spot syndrome virus (WSSV) infection, the P44 group had a notably higher survival rate than the P32 group (p \u2264 0.05). Digestive enzyme activity and antioxidant levels in L. vannamei initially increased and then decreased as protein levels increased, usually peaking in the P40 or P44 groups. Lower dietary protein levels significantly reduced the relative abundance of beneficial bacteria and increased the relative abundance of pathogenic bacteria in the intestines of L. vannamei. Transcriptome sequencing analysis revealed that most differentially expressed genes (DEGs) were up-regulated and then down-regulated as dietary protein levels increased. Furthermore, KEGG pathway enrichment analysis indicated that several immune and metabolic pathways, including metabolic pathways, glutathione metabolism, cytochrome P450, and lysosome and pancreatic secretion, were significantly enriched. In summary, the optimal feed protein level for L. vannamei shrimp was 40\u201344%. Inappropriate feed protein levels reduced antioxidant levels and digestive enzyme activity and promoted pathogen settlement, deceasing factors in various metabolic pathways that respond to microorganisms through transcriptional regulation. This could lead to stunted growth in L. vannamei and compromise their immune function.<\/jats:p>","DOI":"10.3390\/ani14030372","type":"journal-article","created":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T07:42:16Z","timestamp":1706082136000},"page":"372","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Investigating the Impact of Varied Dietary Protein Levels on Litopenaeus vannamei: An Exploration of the Intestinal Microbiota and Transcriptome Responses"],"prefix":"10.3390","volume":"14","author":[{"given":"Gongyu","family":"Li","sequence":"first","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hang","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhibin","family":"Fu","sequence":"additional","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinghui","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihao","family":"Xue","sequence":"additional","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang 524088, China"},{"name":"Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang 524088, China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1111\/j.1365-2109.2007.01704.x","article-title":"Expanding the Utilization of Sustainable Plant Products in Aquafeeds: A Review","volume":"38","author":"Gatlin","year":"2007","journal-title":"Aquac. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"735788","DOI":"10.1016\/j.aquaculture.2020.735788","article-title":"Purple Bacteria as Added-Value Protein Ingredient in Shrimp Feed: Penaeus vannamei Growth Performance, and Tolerance against vibrio and Ammonia Stress","volume":"530","author":"Alloul","year":"2021","journal-title":"Aquaculture"},{"key":"ref_3","first-page":"20","article-title":"Effects of replacing fishmeal with high-protein cotton meal on growth performance, non-specific im-mune indexes, and disease resistance of Penaeus vannamei","volume":"4","author":"Haitao","year":"2018","journal-title":"J. Guangdong Ocean. Univ."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/are.14357","article-title":"Improvement of Nutritional Quality of Live Feed for Aquaculture: An Overview","volume":"51","author":"AkbarAli","year":"2020","journal-title":"Aquac. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"114462","DOI":"10.1016\/j.anifeedsci.2020.114462","article-title":"Survival and Protein Synthesis of Post-Larval White shrimp, Litopenaeus vannamei Were Affected by Dietary Protein Level","volume":"263","author":"Xie","year":"2020","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.aquaeng.2013.04.006","article-title":"Effect of Different Biofloc Levels on Microbial Activity, Water Quality and Performance of Litopenaeus vannamei in a Tank System Operated with No Water Exchange","volume":"56","author":"Schveitzer","year":"2013","journal-title":"Aquac. Eng."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1111\/raq.12316","article-title":"Metabolomics and Fish Nutrition: A Review in the Context of Sustainable Feed Development","volume":"12","author":"Roques","year":"2020","journal-title":"Rev. Aquac."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1046\/j.1365-2095.2003.00235.x","article-title":"Dietary Protein Level and Natural Food Management in the Culture of Blue (Litopenaeus stylirostris) and White Shrimp (Litopenaeus vannamei) in Microcosms","volume":"9","year":"2003","journal-title":"Aquac. Nutr."},{"key":"ref_9","unstructured":"Yearbook, F. (2019). Fishery and Aquaculture Statistics 2019\/FAO Annuaire. Statistiques Des P\u00eaches et de L\u2019aquaculture 2019\/FAO Anuario, FAO."},{"key":"ref_10","first-page":"9","article-title":"Effects of small peptides on growth, antioxidant capacity, non-specific immunity, and intestinal flora structure of Penaeus vannamei","volume":"5","author":"Wu","year":"2021","journal-title":"J. Guangdong Ocean. Univ."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.aquaculture.2014.02.003","article-title":"Evaluation of Dietary Protein Level on Selected Parameters of Immune and Antioxidant Systems, and Growth Performance of Juvenile Litopenaeus vannamei Reared in Zero-Water Exchange Biofloc-Based Culture Tanks","volume":"426\u2013427","author":"Xu","year":"2014","journal-title":"Aquaculture"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.aquaculture.2009.02.034","article-title":"Activity of Trypsin from Litopenaeus vannamei","volume":"290","year":"2009","journal-title":"Aquaculture"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1163\/1937240X-00002267","article-title":"Evaluation of Optimum Dietary Protein Level for Juvenile Whiteleg Shrimp (Litopenaeus vannamei)","volume":"34","author":"Jang","year":"2014","journal-title":"J. Crustac. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.aquaculture.2014.05.005","article-title":"Protein Levels for Litopenaeus vannamei in Semi-Intensive and Biofloc Systems","volume":"432","author":"Vieira","year":"2014","journal-title":"Aquaculture"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.fsi.2021.02.011","article-title":"Effects of Dietary Synbiotics Supplementation Methods on Growth, Intestinal Health, Non-Specific Immunity and Disease Resistance of Pacific White Shrimp, Litopenaeus Vannamei","volume":"112","author":"Yao","year":"2021","journal-title":"Fish. Shellfish. Immunol."},{"key":"ref_16","first-page":"21","article-title":"In-fluence of yeast cultures on the structure of intestinal flora of Penaeus vannamei","volume":"4","author":"Yuanfa","year":"2017","journal-title":"J. Guangdong Ocean. Univ."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Qiao, Y., Zhou, L., Qu, Y., Lu, K., Han, F., and Li, E. (2022). Effects of Different Dietary \u03b2-Glucan Levels on Antioxidant Capacity and Immunity, Gut Microbiota and Transcriptome Responses of White Shrimp (Litopenaeus vannamei) under Low Salinity. Antioxidants, 11.","DOI":"10.3390\/antiox11112282"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"739818","DOI":"10.1016\/j.aquaculture.2023.739818","article-title":"Integrating Microbiome and Transcriptome Analyses to Understand the Effect of Replacing Fishmeal with Tenebrio molitor Meal in Pacific White Shrimp (Litopenaeus vannamei) Diets","volume":"575","author":"Zheng","year":"2023","journal-title":"Aquaculture"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"260","DOI":"10.2174\/1389200221666200303113830","article-title":"Chemical Metabolism of Xenobiotics by Gut Microbiota","volume":"21","author":"Nakov","year":"2020","journal-title":"Curr. Drug. Metab."},{"key":"ref_20","first-page":"27","article-title":"Comparison of bacterial community structure in nursery pond water during the mysid stage of healthy and morbid Penaeus Vannamei","volume":"4","author":"Huang","year":"2018","journal-title":"J. Guangdong Ocean. Univ."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.mib.2016.10.003","article-title":"Microbiome, Metabolites and Host Immunity","volume":"35","author":"Levy","year":"2017","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_22","first-page":"92","article-title":"Ef-fects of chilled fish replacement by combination feeds on the growth and liver transcriptome of Seriola dumerili juveniles","volume":"5","author":"Yang","year":"2023","journal-title":"J. Guangdong Ocean. Univ."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"127513","DOI":"10.1016\/j.jhazmat.2021.127513","article-title":"Impact of Imidacloprid Exposure on the Biochemical Responses, Transcriptome, Gut Microbiota and Growth Performance of the Pacific White Shrimp Litopenaeus Vannamei","volume":"424","author":"Fu","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"154799","DOI":"10.1016\/j.scitotenv.2022.154799","article-title":"Combined Toxic Effects of Thiamethoxam on Intestinal Flora, Transcriptome and Physiology of Pacific White Shrimp Litopenaeus vannamei","volume":"830","author":"Fu","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"101399","DOI":"10.1016\/j.aqrep.2022.101399","article-title":"Schizochytrium limacinum Altered Antioxidant Capacity and Transcriptome Profiles in Pacific White Shrimp Fed a Low-Fishmeal Diet","volume":"27","author":"Xu","year":"2022","journal-title":"Aquac. Rep."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/j.fsi.2021.12.028","article-title":"Phenylalanine Hydroxylase (PAH) Plays a Positive Role during WSSV and Vibrio parahaemolyticus Infection in Litopenaeus vannamei","volume":"120","author":"Yao","year":"2022","journal-title":"Fish Shellfish Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"740469","DOI":"10.1016\/j.aquaculture.2023.740469","article-title":"Tapping Chlorella Vulgaris Potential for Enhanced Growth, Immunity, Digestion, Microbiota, and Immunometabolism in Litopenaeus vannamei Feeding across Varied Salinities","volume":"581","author":"Chen","year":"2024","journal-title":"Aquaculture"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1038\/nmeth.2604","article-title":"UPARSE: Highly Accurate OTU Sequences from Microbial Amplicon Reads","volume":"10","author":"Edgar","year":"2013","journal-title":"Nat. Methods"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5261","DOI":"10.1128\/AEM.00062-07","article-title":"Naive Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy","volume":"73","author":"Wang","year":"2007","journal-title":"Appl. Env. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"7188","DOI":"10.1093\/nar\/gkm864","article-title":"SILVA: A Comprehensive Online Resource for Quality Checked and Aligned Ribosomal RNA Sequence Data Compatible with ARB","volume":"35","author":"Pruesse","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2882","DOI":"10.1093\/bioinformatics\/btv287","article-title":"Tax4Fun: Predicting Functional Profiles from Metagenomic 16S rRNA Data","volume":"31","author":"Wemheuer","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nmeth.f.303","article-title":"QIIME Allows Analysis of High-Throughput Community Sequencing Data","volume":"7","author":"Caporaso","year":"2010","journal-title":"Nat. Methods"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0044-8486(01)00649-4","article-title":"Protein Requirement for Maintenance and Maximum Weight Gain for the Pacific White Shrimp, Litopenaeus vannamei","volume":"204","author":"Kureshy","year":"2002","journal-title":"Aquaculture"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1111\/j.1365-2109.2005.01287.x","article-title":"Effect of Dietary Protein Level on Growth, Survival and Ammonia Efflux Rate of Litopenaeus vannamei (Boone) Raised in a Zero Water Exchange Culture System","volume":"36","year":"2005","journal-title":"Aquac. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1111\/anu.13166","article-title":"Effects of Different Levels of Dietary Protein on the Growth Performance, Nutrient Utilization, Digestive Enzymes and Physiological Status of White Shrimp, Litopenaeus vannamei Juveniles Reared in Inland Saline Water","volume":"27","author":"Talukdar","year":"2021","journal-title":"Aquac. Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1111\/j.1365-2109.2010.02585.x","article-title":"Influence of Dietary Protein Levels on Growth, Digestibility, Digestive Enzyme Activity and Stress Tolerance in White-Leg Shrimp, Litopenaeus vannamei (Boone, 1931), Reared in High-Density Tank Trials","volume":"41","author":"Xia","year":"2010","journal-title":"Aquac. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"628","DOI":"10.3382\/japr.2013-00739","article-title":"Feed Enzymes: The Science, Practice, and Metabolic Realities1 1Presented as a Part of the Informal Nutrition Symposium \u201cMetabolic Responses to Nutrition and Modifiers\u201d at the Poultry Science Association\u2019s Annual Meeting in Athens, Georgia, July 9, 2012","volume":"22","author":"Ravindran","year":"2013","journal-title":"J. Appl. Poult. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.fsi.2019.04.043","article-title":"Transcriptome Signatures of the Pacific White Shrimp Litopenaeus vannamei Hepatopancreas in Response to Stress in Biofloc Culture Systems","volume":"91","author":"Xue","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"6192","DOI":"10.1016\/j.biortech.2009.06.102","article-title":"Evaluation of Gracilaria Caudata J. Agardh for Bioremediation of Nutrients from Shrimp Farming Wastewater","volume":"100","author":"Panucci","year":"2009","journal-title":"Bioresour. Technol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1111\/j.1365-2109.2011.02883.x","article-title":"Partial Replacement of Fish Meal with Peanut Meal in Practical Diets for the Pacific White Shrimp, Litopenaeus vannamei","volume":"43","author":"Liu","year":"2012","journal-title":"Aquac. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0952-7915(98)80026-5","article-title":"Role of the Prophenoloxidase-Activating System in Invertebrate Immunity","volume":"10","author":"Cerenius","year":"1998","journal-title":"Curr. Opin. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.fsi.2020.09.016","article-title":"Effects of Phyllanthus amarus Extract on Nonspecific Immune Responses, Growth, and Resistance to Vibrio alginolyticus in White Shrimp Litopenaeus vannamei","volume":"107","author":"Ngo","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1111\/j.1365-2109.2011.03013.x","article-title":"Vibrio alginolyticus Infection in Asian Seabass (Lates calcarifer, Bloch) Reared in Open Sea Floating Cages in India","volume":"44","author":"Rathore","year":"2012","journal-title":"Aquac. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"736771","DOI":"10.1016\/j.aquaculture.2021.736771","article-title":"Immune Function of Cytosolic Manganese Superoxide Dismutase from Macrobrachium rosenbergii in Response to Bacterial Infection","volume":"541","author":"Li","year":"2021","journal-title":"Aquaculture"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aquatox.2014.10.014","article-title":"Toxicity of Nano-TiO2 on Algae and the Site of Reactive Oxygen Species Production","volume":"158","author":"Li","year":"2015","journal-title":"Aquat. Toxicol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1038\/nature07540","article-title":"A Core Gut Microbiome in Obese and Lean Twins","volume":"457","author":"Turnbaugh","year":"2009","journal-title":"Nature"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2218","DOI":"10.1111\/anu.13357","article-title":"Effects of Two Dietary Protein Levels on Growth, Body Composition, Intestinal Microflora and Expression of TOR, IGF-I, LPL and HSP70 of Juvenile Silver Sillago Sillago Sihama","volume":"27","author":"Liu","year":"2021","journal-title":"Aquac. Nutr."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"4619","DOI":"10.1111\/are.15296","article-title":"Replacement of Fish Meal by Enzyme-Treated Soybean on the Growth Performance, Intestine Microflora, Immune Responses and Disease Resistance of Pacific White Shrimp Litopenaeus vannamei","volume":"52","author":"Fan","year":"2021","journal-title":"Aquac. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1111\/are.12628","article-title":"Changes in the Intestinal Bacterial Community during the Growth of White Shrimp, Litopenaeus vannamei","volume":"47","author":"Huang","year":"2016","journal-title":"Aquac. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.fsi.2020.04.053","article-title":"The Immune Defense Response of Pacific White Shrimp (Litopenaeus vannamei) to Temperature Fluctuation","volume":"103","author":"Wang","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.tibtech.2015.06.011","article-title":"Proteobacteria: Microbial Signature of Dysbiosis in Gut Microbiota","volume":"33","author":"Shin","year":"2015","journal-title":"Trends Biotechnol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S0044-8486(00)00458-0","article-title":"Relationships between Disease Outbreak in Cultured Tiger Shrimp (Penaeus monodon) and the Composition of Vibrio Communities in Pond Water and Shrimp Hepatopancreas during Cultivation","volume":"192","author":"Sung","year":"2001","journal-title":"Aquaculture"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1006\/jipa.2001.5073","article-title":"Differences in the Susceptibility of American White Shrimp Larval Substages (Litopenaeus vannamei) to Four Vibrio Species","volume":"78","author":"Ascencio","year":"2001","journal-title":"J. Invertebr. Pathol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"100543","DOI":"10.1016\/j.aqrep.2020.100543","article-title":"Acinetobacter Venetianus, a Potential Pathogen of Red Leg Disease in Freshwater-Cultured Whiteleg Shrimp Penaeus vannamei","volume":"18","author":"Huang","year":"2020","journal-title":"Aquac. Rep."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.anifeedsci.2018.09.002","article-title":"Effects of Extruded Aquafeed on Growth Performance and Gut Microbiome of Juvenile Totoaba macdonaldi","volume":"245","author":"Viana","year":"2018","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"6464","DOI":"10.1038\/s41598-019-42939-8","article-title":"Changes in the Intestine Microbial, Digestion and Immunity of Litopenaeus vannamei in Response to Dietary Resistant Starch","volume":"9","author":"Duan","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_57","unstructured":"Bhattacharya, S. (2015). Free Radicals in Human Health and Disease, Springer."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/s40071-018-0208-3","article-title":"Effect of Dietary Seaweed Supplementation on Growth Performance, Antioxidant and Immune Responses in European Seabass (Dicentrarchus labrax) Subjected to Rearing Temperature and Salinity Oscillations","volume":"10","author":"Lobo","year":"2018","journal-title":"Int. Aquat. Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"105233","DOI":"10.1016\/j.aquatox.2019.105233","article-title":"Impacts of Acute Toxicity of Arsenic (III) Alone and with High Temperature on Stress Biomarkers, Immunological Status and Cellular Metabolism in Fish","volume":"214","author":"Kumar","year":"2019","journal-title":"Aquat. Toxicol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.ecoenv.2019.05.029","article-title":"Stress Response and Tolerance Mechanisms of Ammonia Exposure Based on Transcriptomics and Metabolomics in Litopenaeus vannamei","volume":"180","author":"Xiao","year":"2019","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_61","first-page":"263","article-title":"Basic Review of the Cytochrome P450 System","volume":"4","author":"McDonnell","year":"2013","journal-title":"J. Adv. Pract. Oncol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.etap.2012.02.004","article-title":"Cytochrome P450 (CYP) in Fish","volume":"34","author":"Uno","year":"2012","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Liu, C., Li, J., Qi, X., Wang, L., Sun, D., Zhang, J., Zhang, K., Li, J., Li, Y., and Wen, H. (2023). Cytochrome P450 Superfamily in Spotted Sea Bass: Genome-Wide Identification and Expression Profiles under Trichlorfon and Environmental Stresses. Comp. Biochem. Physiol. Part D Genom. Proteom., 46.","DOI":"10.1016\/j.cbd.2023.101078"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/S0166-445X(01)00173-4","article-title":"Environmental Endocrine Disruption in Decapod Crustacean Larvae: Hormone Titers, Cytochrome P450, and Stress Protein Responses to Heptachlor Exposure","volume":"55","author":"Snyder","year":"2001","journal-title":"Aquat. Toxicol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1093\/jmcb\/mjt022","article-title":"The Lysosome: From Waste Bag to Potential Therapeutic Target","volume":"5","author":"Appelqvist","year":"2013","journal-title":"J. Mol. Cell Biol."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Saftig, P. (2005). Lysosomes, Springer US. Medical Intelligence Unit.","DOI":"10.1007\/0-387-28957-7"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1002\/jmor.21040","article-title":"Functional Cytology of the Hepatopancreas of Decapod Crustaceans","volume":"280","author":"Vogt","year":"2019","journal-title":"J. Morphol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.fsi.2021.10.005","article-title":"Dietary Effects of Clostridium Autoethanogenum Protein Substituting Fish Meal on Growth, Intestinal Histology and Immunity of Pacific White Shrimp (Litopenaeus vannamei) Based on Transcriptome Analysis","volume":"119","author":"Jiang","year":"2021","journal-title":"Fish Shellfish Immunol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"115775","DOI":"10.1016\/j.taap.2021.115775","article-title":"RNA-Seq Unveiled Section-Specific Host Response to Lack of Gut Microbiota in Mouse Intestine","volume":"433","author":"Fu","year":"2021","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_70","first-page":"37","article-title":"RNA-Seq Reveals the Dynamic and Diverse Features of Digestive Enzymes during Early Development of Pacific White Shrimp Litopenaeus vannamei","volume":"11","author":"Wei","year":"2014","journal-title":"Comp. Biochem. Physiol. Part D Genom. Proteom."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"4497","DOI":"10.1021\/pr5005746","article-title":"Carboxyterminal Protein Processing in Health and Disease: Key Actors and Emerging Technologies","volume":"13","author":"Petrera","year":"2014","journal-title":"J. Proteome Res."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1163\/193724012X634206","article-title":"Comparative Study of Amylases from the Midgut Gland of Three Species of Penaeid Shrimp","volume":"32","author":"Castro","year":"2012","journal-title":"J. Crustac. Biol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.ceca.2019.04.005","article-title":"Emerging Roles for Multifunctional Ion Channel Auxiliary Subunits in Cancer","volume":"80","author":"Haworth","year":"2019","journal-title":"Cell Calcium"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1076\/ceyr.21.6.918.6983","article-title":"Isolation and Characterization of a Ca2+-Activated Chloride Channel from Human Corneal Epithelium","volume":"21","author":"Itoh","year":"2000","journal-title":"Curr. Eye Res."},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Sharma, A., Ramena, G., Yin, Y., Premkumar, L., and Elble, R.C. (2018). CLCA2 Is a Positive Regulator of Store-Operated Calcium Entry and TMEM16A. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0196512"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/3\/372\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:48:17Z","timestamp":1760104097000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/3\/372"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,24]]},"references-count":75,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,2]]}},"alternative-id":["ani14030372"],"URL":"https:\/\/doi.org\/10.3390\/ani14030372","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,24]]}}}