{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:05:27Z","timestamp":1787616327373,"version":"build-2736575974"},"reference-count":69,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T00:00:00Z","timestamp":1706486400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Slovenian Research and Innovation Agency (ARIS)","award":["P3\u20130003"],"award-info":[{"award-number":["P3\u20130003"]}]},{"name":"Slovenian Research and Innovation Agency (ARIS)","award":["P4\u20130053"],"award-info":[{"award-number":["P4\u20130053"]}]},{"name":"Slovenian Research and Innovation Agency (ARIS)","award":["J4\u20132546"],"award-info":[{"award-number":["J4\u20132546"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Electrochemotherapy (ECT) in combination with the gene electrotransfer of interleukin 12 (IL-12 GET) has been successfully used in veterinary medicine for the treatment of mast cell tumours (MCT), but the biomarkers that could predict response to this treatment have not yet been investigated. The aim of this study was to determine the plasma nucleosome and serum ferritin concentrations, as well as the lactate dehydrogenase (LDH) activity, in the serum of treated patients before and one and six months after treatment to evaluate their utility as potential biomarkers that could predict response to the combined treatment. The study was conducted in 48 patients with a total of 86 MCTs that we treated with the combined treatment. The blood samples used for analysing the potential predictive biomarkers were taken before treatment and one and six months after treatment, when the response to treatment was also assessed. The Nu. Q\u00ae Vet Cancer Test, the Canine Ferritin ELISA Kit, and the RX Daytona+ automated biochemical analyser were used to analyse the blood samples. The results showed that the plasma nucleosome concentration (before treatment (BT): 32.84 ng\/mL (median); one month after treatment (1 M AT): 58.89 ng\/mL (median); p = 0.010) and serum LDH activity (BT: 59.75 U\/L (median); 1 M AT: 107.5 U\/L (median); p = 0.012) increased significantly one month after treatment and that the increase correlated significantly with the presence of a more pronounced local reaction (necrosis, swelling, etc.) at that time point for both markers (nucleosome: BT (necrosis): 21.61 ng\/mL (median); 1 M AT (necrosis): 69.92 ng\/mL (median), p = 0.030; LDH: BT (necrosis): 54.75 U\/L (median); 1 M AT (necrosis): 100.3 U\/L (median), p = 0.048). Therefore, both the plasma nucleosome concentration and serum LDH activity could serve as early indicators of the effect of the treatment. In this context, the serum ferritin concentration showed no significant predictive potential for treatment response (p &gt; 0.999 for all comparisons). In conclusion, this study provides some new and important observations on the use of predictive biomarkers in veterinary oncology. Furthermore, it emphasises the need for the continued identification and validation of potential predictive biomarkers in dogs with MCT and other malignancies undergoing ECT treatment in combination with IL-12 GET to ultimately improve treatment outcomes.<\/jats:p>","DOI":"10.3390\/ani14030438","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T12:25:01Z","timestamp":1706531101000},"page":"438","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparison of Nucleosome, Ferritin and LDH Levels in Blood with Clinical Response before and after Electrochemotherapy Combined with IL-12 Gene Electrotransfer for the Treatment of Mast Cell Tumours in Dogs"],"prefix":"10.3390","volume":"14","author":[{"given":"Ma\u0161a","family":"Vilfan","sequence":"first","affiliation":[{"name":"Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2905-4574","authenticated-orcid":false,"given":"Ur\u0161a","family":"Lampreht Tratar","sequence":"additional","affiliation":[{"name":"Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia"},{"name":"Institute of Oncology Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Milevoj","sequence":"additional","affiliation":[{"name":"Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1199-4501","authenticated-orcid":false,"given":"Alenka","family":"Nemec Svete","sequence":"additional","affiliation":[{"name":"Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1418-1928","authenticated-orcid":false,"given":"Maja","family":"\u010cema\u017ear","sequence":"additional","affiliation":[{"name":"Institute of Oncology Ljubljana, 1000 Ljubljana, Slovenia"},{"name":"Faculty of Health Sciences, University of Primorska, 6310 Izola, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7641-5670","authenticated-orcid":false,"given":"Gregor","family":"Ser\u0161a","sequence":"additional","affiliation":[{"name":"Institute of Oncology Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nata\u0161a","family":"Tozon","sequence":"additional","affiliation":[{"name":"Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1111\/vco.12208","article-title":"Efficacy and safety of electrochemotherapy combined with peritumoral IL-12 gene electrotransfer of canine mast cell tumours","volume":"15","author":"Cemazar","year":"2017","journal-title":"Veter. Comp. Oncol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1111\/jcmm.12382","article-title":"Safe and effective treatment of spontaneous neoplasms with interleukin 12 electro-chemo-gene therapy","volume":"19","author":"Cutrera","year":"2015","journal-title":"J. Cell. Mol. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.rvsc.2018.11.001","article-title":"A combination of electrochemotherapy, gene electrotransfer of plasmid encoding canine IL-12 and cytoreductive surgery in the treatment of canine oral malignant melanoma","volume":"122","author":"Milevoj","year":"2019","journal-title":"Res. Veter. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1515\/raon-2017-0035","article-title":"Effects of Electrochemotherapy with Cisplatin and Peritumoral IL-12 Gene Electrotransfer on Canine Mast Cell Tumors: A Histopathologic and Immunohistochemical Study","volume":"51","author":"Salvadori","year":"2017","journal-title":"Radiol. Oncol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1038\/cgt.2010.13","article-title":"Bleomycin\/interleukin-12 electrochemogenetherapy for treating naturally occurring spontaneous neoplasms in dogs","volume":"17","author":"Reed","year":"2010","journal-title":"Cancer Gene Ther."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"110274","DOI":"10.1016\/j.intimp.2023.110274","article-title":"Treatment of spontaneous canine mast cell tumors by electrochemotherapy combined with IL-12 gene electrotransfer: Comparison of intratumoral and peritumoral application of IL-12","volume":"120","author":"Milevoj","year":"2023","journal-title":"Int. Immunopharmacol."},{"key":"ref_7","first-page":"839","article-title":"Effective treatment of perianal tumors in dogs with electrochemotherapy","volume":"25","author":"Tozon","year":"2005","journal-title":"Anticancer. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"141","DOI":"10.2478\/raon-2023-0029","article-title":"Bleomycin electrosclerotherapy (BEST) for the treatment of vascular malformations. An International Network for Sharing Practices on Electrochemotherapy (InspECT) study group report","volume":"57","author":"Muir","year":"2023","journal-title":"Radiol. Oncol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/S0305-7372(03)00073-2","article-title":"Electrochemotherapy: Results of cancer treatment using enhanced delivery of bleomycin by electroporation","volume":"29","author":"Gothelf","year":"2003","journal-title":"Cancer Treat. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1097\/00008390-200012000-00011","article-title":"Efficient palliation of haemorrhaging malignant melanoma skin metastases by electrochemotherapy","volume":"10","author":"Gehl","year":"2000","journal-title":"Melanoma Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"347","DOI":"10.2478\/raon-2020-0038","article-title":"Percutaneous image guided electrochemotherapy of hepatocellular carcinoma: Technological advancement","volume":"54","author":"Djokic","year":"2020","journal-title":"Radiol. Oncol."},{"key":"ref_12","unstructured":"Withrow, S.J., Vail, D.M., and Page, R.L. (2020). Withrow and MacEwen\u2019s Small Animal Clinical Oncology, Elsevier."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e28131","DOI":"10.4161\/onci.28131","article-title":"Electrochemotherapy with bleomycin induces hallmarks of immunogenic cell death in murine colon cancer cells","volume":"3","author":"Calvet","year":"2014","journal-title":"OncoImmunology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.canlet.2018.11.015","article-title":"Electrochemotherapy with cisplatin increases survival and induces immunogenic responses in murine models of lung cancer and colorectal cancer","volume":"442","author":"Tremble","year":"2019","journal-title":"Cancer Lett."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/j.jconrel.2021.03.009","article-title":"Potentiation of electrochemotherapy effectiveness by immunostimulation with IL-12 gene electrotransfer in mice is dependent on tumor immune status","volume":"332","author":"Ursic","year":"2021","journal-title":"J. Control. Release"},{"key":"ref_16","first-page":"391","article-title":"Effective treatment of multiple unresectable skin melanoma metastases by electrochemotherapy","volume":"48","author":"Snoj","year":"2007","journal-title":"Croat. Med. J."},{"key":"ref_17","first-page":"5285890","article-title":"Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma","volume":"2017","author":"Loiacono","year":"2017","journal-title":"Mediat. Inflamm."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"398","DOI":"10.2478\/raon-2022-0021","article-title":"Treatment of skin tumors with intratumoral interleukin 12 gene electrotransfer in the head and neck region: A first-in-human clinical trial protocol","volume":"56","author":"Groselj","year":"2022","journal-title":"Radiol. Oncol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5896","DOI":"10.1200\/JCO.2007.15.6794","article-title":"Phase I trial of interleukin-12 plasmid electroporation in patients with metastatic melanoma","volume":"26","author":"Daud","year":"2008","journal-title":"J. Clin. Oncol."},{"key":"ref_20","first-page":"e1","article-title":"European consensus document on mast cell tumours in dogs and cats","volume":"10","author":"Blackwood","year":"2012","journal-title":"Veter. Comp. Oncol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1111\/vco.12032","article-title":"Response evaluation criteria for solid tumours in dogs (v1.0): A Veterinary Cooperative Oncology Group (VCOG) consensus document","volume":"13","author":"Nguyen","year":"2015","journal-title":"Veter. Comp. Oncol."},{"key":"ref_22","first-page":"55","article-title":"Electrochemotherapy compared to surgery for treatment of canine mast cell tumours","volume":"23","author":"Kodre","year":"2009","journal-title":"Vivo"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.1016\/j.ejso.2021.03.229","article-title":"Biological factors of the tumour response to electrochemotherapy: Review of the evidence and a research roadmap","volume":"47","author":"Sersa","year":"2021","journal-title":"Eur. J. Surg. Oncol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1146\/annurev-animal-021815-111431","article-title":"Biomarkers in Veterinary Medicine","volume":"5","author":"Myers","year":"2017","journal-title":"Annu. Rev. Anim. Biosci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1186\/s40425-019-0799-2","article-title":"Peripheral immune-based biomarkers in cancer immunotherapy: Can we realize their predictive potential?","volume":"7","author":"Nixon","year":"2019","journal-title":"J. Immunother. Cancer"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1111\/j.1939-1676.2010.0543.x","article-title":"Evaluation of serum ferritin as a tumor marker for canine histiocytic sarcoma","volume":"24","author":"Friedrichs","year":"2010","journal-title":"J. Veter. Intern. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1111\/j.1939-1676.2001.tb01595.x","article-title":"Serum zinc, chromium, and iron concentrations in dogs with lymphoma and osteosarcoma","volume":"15","author":"Kazmierski","year":"2001","journal-title":"J. Vet. Intern. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"849","DOI":"10.2460\/javma.1994.205.06.849","article-title":"Hyperferritinemia associated with malignant histiocytosis in a dog","volume":"205","author":"Newlands","year":"1994","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"6651","DOI":"10.1038\/s41598-021-85029-4","article-title":"Combined detection of CA15-3, CEA, and SF in serum and tissue of canine mammary gland tumor patients","volume":"11","author":"Fan","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1111\/j.1939-1676.2012.01014.x","article-title":"CA15.3, CEA and LDH in Dogs with Malignant Mammary Tumors","volume":"26","author":"Campos","year":"2012","journal-title":"J. Veter. Intern. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1111\/j.1476-5829.2009.00196.x","article-title":"Serum lactate dehydrogenase activity in canine malignancies","volume":"7","author":"Marconato","year":"2009","journal-title":"Veter. Comp. Oncol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1023\/B:VERC.0000014201.82393.67","article-title":"Diagnostic and prognostic value of serum lactate dehydrogenase (LDH) and LDH isoenzymes in canine lymphoma","volume":"27","author":"Zanatta","year":"2003","journal-title":"Veter. Res. Commun."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"283","DOI":"10.56808\/2985-1130.2993","article-title":"The expression of serum lactate dehydrogenase in canine oral tumors","volume":"49","author":"Choisunirachon","year":"2019","journal-title":"Thai J. Veter. Med."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Murray, B., and Blacklock, K.L.B. (2022). Pilot Study: Assessing the Expression of Serum Lactate Dehydrogenase and Peripheral Leukocyte Ratios in Canine Oral Malignant Melanoma. Veter. Sci., 9.","DOI":"10.3390\/vetsci9080421"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Wilson-Robles, H.M., Bygott, T., Kelly, T.K., Miller, T.M., Miller, P., Matsushita, M., Terrell, J., Bougoussa, M., and Butera, T. (2022). Evaluation of plasma nucleosome concentrations in dogs with a variety of common cancers and in healthy dogs. BMC Veter. Res., 18.","DOI":"10.1186\/s12917-022-03429-8"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Wilson-Robles, H., Miller, T., Jarvis, J., Terrell, J., Dewsbury, N., Kelly, T., Herzog, M., Bygott, T., Hardat, N., and Michel, G. (2020). Evaluation of nucleosome concentrations in healthy dogs and dogs with cancer. PLoS ONE, 15.","DOI":"10.1101\/2020.07.08.193466"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"208","DOI":"10.3389\/fvets.2019.00208","article-title":"Biomarker Guided Diagnosis of Septic Peritonitis in Dogs","volume":"6","author":"Martiny","year":"2019","journal-title":"Front. Veter. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1111\/vec.12773","article-title":"Determining prognosis in canine sepsis by bedside measurement of cell-free DNA and nucleosomes","volume":"28","author":"Letendre","year":"2018","journal-title":"J. Veter. Emerg. Crit. Care"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"180","DOI":"10.3389\/fvets.2018.00180","article-title":"Concentrations of Plasma Nucleosomes but Not Cell-Free DNA Are Prognostic in Dogs Following Trauma","volume":"5","author":"Letendre","year":"2018","journal-title":"Front. Veter. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Dolan, C., Miller, T., Jill, J., Terrell, J., Kelly, T.K., Bygott, T., and Wilson-Robles, H. (2021). Characterizing circulating nucleosomes in the plasma of dogs with lymphoma. BMC Veter. Res., 17.","DOI":"10.1186\/s12917-021-02991-x"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"10247","DOI":"10.18632\/oncotarget.21908","article-title":"For the Danish Collaborative Group on Early Detection of Colorectal Cancer Circulating cell-free nucleosomes as biomarkers for early detection of colorectal cancer","volume":"9","author":"Rasmussen","year":"2018","journal-title":"Oncotarget"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"36","DOI":"10.3816\/CLC.2010.n.006","article-title":"Plasma Nucleosome Levels Might Predict Response to Therapy in Patients with Advanced Non\u2013Small-Cell Lung Cancer","volume":"11","author":"Kumar","year":"2010","journal-title":"Clin. Lung Cancer"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Tsoneva, D.K., Ivanov, M.N., Conev, N.V., Manev, R., Stoyanov, D.S., and Vinciguerra, M. (2023). Circulating Histones to Detect and Monitor the Progression of Cancer. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24020942"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.rvsc.2020.10.004","article-title":"Nucleolin as a potential biomarker for canine malignant neoplasia","volume":"135","author":"Cruz","year":"2021","journal-title":"Res. Veter. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1007\/s00018-005-5285-y","article-title":"Cellular regulation and molecular interactions of the ferritins","volume":"63","author":"Hintze","year":"2006","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1111\/bjh.15166","article-title":"Investigation and management of a raised serum ferritin","volume":"181","author":"Cullis","year":"2018","journal-title":"Br. J. Haematol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1056\/NEJM197505012921805","article-title":"Ferritin in serum: Clinical and biochemical implications","volume":"292","author":"Jacobs","year":"1975","journal-title":"N. Engl. J. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/0268-960X(90)90006-E","article-title":"Ferritin","volume":"4","author":"Worwood","year":"1990","journal-title":"Blood Rev."},{"key":"ref_49","first-page":"314","article-title":"Ferritin: A potential serum marker for lymph node metastasis in head and neck squamous cell carcinoma","volume":"17","author":"Hu","year":"2018","journal-title":"Oncol. Lett."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Jung, M., Mertens, C., Tomat, E., and Br\u00fcne, B. (2019). Iron as a Central Player and Promising Target in Cancer Progression. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20020273"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.tibs.2005.01.005","article-title":"Multifaceted roles of glycolytic enzymes","volume":"30","author":"Kim","year":"2005","journal-title":"Trends Biochem. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"6124","DOI":"10.1002\/cam4.1820","article-title":"Lactate dehydrogenase A: A key player in carcinogenesis and potential target in cancer therapy","volume":"7","author":"Feng","year":"2018","journal-title":"Cancer Med."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1177\/0300985817747325","article-title":"Assessment of Canine Mast Cell Tumor Mortality Risk Based on Clinical, Histologic, Immunohistochemical, and Molecular Features","volume":"55","author":"Horta","year":"2018","journal-title":"Veter. Pathol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1177\/0300985810386469","article-title":"Proposal of a 2-Tier Histologic Grading System for Canine Cutaneous Mast Cell Tumors to More Accurately Predict Biological Behavior","volume":"48","author":"Kiupel","year":"2011","journal-title":"Veter. Pathol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1177\/030098588402100503","article-title":"Canine Cutaneous Mast Cell Tumor: Morphologic Grading and Survival Time in 83 Dogs","volume":"21","author":"Patnaik","year":"1984","journal-title":"Veter. Pathol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1038\/s41417-018-0014-5","article-title":"Antitumor effect of antibiotic resistance gene-free plasmids encoding interleukin-12 in canine melanoma model","volume":"25","author":"Kos","year":"2018","journal-title":"Cancer Gene Ther."},{"key":"ref_57","first-page":"e54760","article-title":"Operating Procedures of the Electrochemotherapy for Treatment of Tumor in Dogs and Cats","volume":"2016","author":"Tozon","year":"2016","journal-title":"J. Vis. Exp."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1111\/vco.12217","article-title":"The treatment of canine mast cell tumours with electrochemotherapy with or without surgical excision","volume":"15","author":"Lowe","year":"2017","journal-title":"Veter. Comp. Oncol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"88","DOI":"10.4314\/ovj.v9i1.15","article-title":"Adjuvant electrochemotherapy with bleomycin and cisplatin combination for canine soft tissue sarcomas: A study of 30 cases","volume":"9","author":"Spugnini","year":"2019","journal-title":"Open Veter. J."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1111\/vco.12462","article-title":"Soft tissue sarcoma in dogs: A treatment review and a novel approach using electrochemotherapy in a case series","volume":"17","author":"Torrigiani","year":"2019","journal-title":"Veter. Comp. Oncol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1002\/ijc.2910200304","article-title":"Increased ferritin synthesis and release by Hodgkin\u2019s disease peripheral blood lymphocytes","volume":"20","author":"Sarcione","year":"1977","journal-title":"Int. J. Cancer"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"AB229","DOI":"10.1016\/j.jaci.2017.12.722","article-title":"Ferritin Particles Accumulate in Human Mast Cell Secretory Granules and Are Released upon Fc\u03b5RI-mediated Activation","volume":"141","author":"Ward","year":"2018","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1111\/vco.12346","article-title":"Diagnostic accuracy of pre-treatment biopsy for grading cutaneous mast cell tumours in dogs","volume":"16","author":"Shaw","year":"2018","journal-title":"Veter. Comp. Oncol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1159\/000094694","article-title":"Nucleosomes in Colorectal Cancer Patients during Radiochemotherapy","volume":"27","author":"Kremer","year":"2006","journal-title":"Tumor Biol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1196\/annals.1318.032","article-title":"Therapy Control in Oncology by Circulating Nucleosomes","volume":"1022","author":"Holdenrieder","year":"2004","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1002\/1097-0215(20010320)95:2<114::AID-IJC1020>3.0.CO;2-Q","article-title":"Nucleosomes in serum of patients with benign and malignant diseases","volume":"95","author":"Holdenrieder","year":"2001","journal-title":"Int. J. Cancer"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Yee, N.S. (2020). Liquid Biopsy: A Biomarker-Driven Tool towards Precision Oncology. J. Clin. Med., 9.","DOI":"10.3390\/jcm9082556"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1177\/0300985816638721","article-title":"Cytologic Criteria for Mast Cell Tumor Grading in Dogs with Evaluation of Clinical Outcome","volume":"53","author":"Camus","year":"2016","journal-title":"Veter. Pathol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1111\/j.1476-5829.2009.00185.x","article-title":"Cytological lymph node evaluation in dogs with mast cell tumours: Association with grade and survival","volume":"7","author":"Krick","year":"2009","journal-title":"Veter. Comp. Oncol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/3\/438\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:51:04Z","timestamp":1760104264000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/3\/438"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,29]]},"references-count":69,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,2]]}},"alternative-id":["ani14030438"],"URL":"https:\/\/doi.org\/10.3390\/ani14030438","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,29]]}}}