{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:14:47Z","timestamp":1791245687365,"version":"4.1.0"},"reference-count":65,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T00:00:00Z","timestamp":1708646400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Terragen biotech Pty Ltd."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The calf\u2019s gastrointestinal tract (GIT) microbiome undergoes rapid shifts during early post-natal life, which can directly affect calf performance. The objectives of this study were to characterise and compare differences in the establishment and succession of GIT microbiota, GIT morphological changes, and the growth of dairy calves from birth until weaned. Forty-four newborn Holstein-Friesian calves were randomly selected and assigned to Treatment (TRT) and Control (CON) groups. The TRT group calves received a once-daily dose of a direct-fed microbial (DFM) liquid product containing Lacticaseibacillus paracasei, Lentilactobacillus buchneri, and Lacticaseibacillus casei, all formerly known as Lactobacillus. Fresh faecal samples were manually taken from the rectum of all calves, and gross necropsy was performed on the forestomachs and gastrointestinal tracts. Bacterial DNA was extracted from frozen faecal samples for 16S rRNA gene amplicon sequencing. Calves in the TRT group had greater live weights (p = 0.02) at weaning compared with calves in the CON group (mean = 69.18 kg, SD = 13.37 kg). The average daily live weight gain (ADG) and total feed intake were similar between the two groups. Calves in the TRT group had greater duodenum, abomasum, and reticulum weights (p = 0.05). Rumen and intestinal development (p &lt; 0.05) and faecal microbial diversity (p &lt; 0.05) were more pronounced in the TRT group. The relative abundances of eight genera differed (p &lt; 0.001) between the groups. Supplementing calves with the LAB-based DFM increased live weight at weaning and had a more pronounced effect on the development of rumen and the gastrointestinal tract and on microbiota diversity and evenness. Future work is needed to better understand the potential association of LAB-DFM products on gut mucosa-associated microbiota.<\/jats:p>","DOI":"10.3390\/ani14050693","type":"journal-article","created":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T06:07:39Z","timestamp":1708668459000},"page":"693","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effect of a Lactobacilli-Based Direct-Fed Microbial Product on Gut Microbiota and Gastrointestinal Morphological Changes"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4314-9123","authenticated-orcid":false,"given":"John I.","family":"Alawneh","sequence":"first","affiliation":[{"name":"School of Veterinary Science, University of Queensland, Gatton, QLD 4343, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hena","family":"Ramay","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T3R 1J3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Olchowy","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T3R 1J3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5126-7125","authenticated-orcid":false,"given":"Rachel","family":"Allavena","sequence":"additional","affiliation":[{"name":"School of Veterinary Science, University of Queensland, Gatton, QLD 4343, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7270-0589","authenticated-orcid":false,"given":"Martin","family":"Soust","sequence":"additional","affiliation":[{"name":"Terragen Biotech Pty Ltd., Coolum Beach, QLD 4573, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafat Al","family":"Jassim","sequence":"additional","affiliation":[{"name":"Queensland Alliance for Agriculture and Food Innovation, St Lucia, QLD 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Du, W., Wang, X., Hu, M., Hou, J., Du, Y., Si, W., Yang, L., Xu, L., and Xu, Q. 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