{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T22:06:50Z","timestamp":1794002810630,"version":"4.8.0"},"reference-count":56,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,3,12]],"date-time":"2024-03-12T00:00:00Z","timestamp":1710201600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET)","award":["122014-2"],"award-info":[{"award-number":["122014-2"]}]},{"name":"Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET)","award":["122059-2"],"award-info":[{"award-number":["122059-2"]}]},{"name":"Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET)","award":["2018R1C1B5033331"],"award-info":[{"award-number":["2018R1C1B5033331"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["122014-2"],"award-info":[{"award-number":["122014-2"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["122059-2"],"award-info":[{"award-number":["122059-2"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["2018R1C1B5033331"],"award-info":[{"award-number":["2018R1C1B5033331"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Since the outbreak of the H9N2\/Y439 avian influenza virus in 1996, the Korean poultry industry has incurred severe economic losses. A novel possibly zoonotic H9N2 virus from the Y280-like lineage (H9N2\/Y280) has been prevalent in Korea since June 2020, posing a threat to the poultry sector. Rapid mutation of influenza viruses urges the development of effective vaccines against newly generated strains. Thus, we engineered a recombinant virus rHVT\/Y280 to combat H9N2\/Y280. We integrated the hemagglutinin (HA) gene of the H9N2\/Y280 strain into the US2 region of the herpesvirus of turkeys (HVT) Fc126 vaccine strain, utilizing CRISPR\/Cas9 gene-editing technology. The successful construction of rHVT\/Y280 was confirmed by polymerase chain reaction and sequencing, followed by efficacy evaluation. Four-day-old specific pathogen-free chickens received the rHVT\/Y280 vaccine and were challenged with the H9N2\/Y280 strain A21-MRA-003 at 3 weeks post-vaccination. In 5 days, there were no gross lesions among the vaccinated chickens. The rHVT\/Y280 vaccine induced strong humoral immunity and markedly reduced virus shedding, achieving 100% inhibition of virus recovery in the cecal tonsil and significantly lowering tissue viral load. Thus, HVT vector vaccines expressing HA can be used for protecting poultry against H9N2\/Y280. The induction of humoral immunity by live vaccines is vital in such cases. In summary, the recombinant virus rHVT\/Y280 is a promising vaccine candidate for the protection of chickens against the H9N2\/Y280.<\/jats:p>","DOI":"10.3390\/ani14060872","type":"journal-article","created":{"date-parts":[[2024,3,12]],"date-time":"2024-03-12T12:17:16Z","timestamp":1710245836000},"page":"872","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Protection of Chickens against H9N2 Avian Influenza Isolates with a Live Vector Vaccine Expressing Influenza Hemagglutinin Gene Derived from Y280 Avian Influenza Virus"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2360-6382","authenticated-orcid":false,"given":"Jun-Feng","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Avian Diseases, College of Veterinary Medicine and Center for Avian Disease, Jeonbuk National University, Iksan 54596, Republic of Korea"},{"name":"College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang-Won","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Avian Diseases, College of Veterinary Medicine and Center for Avian Disease, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Shang","sequence":"additional","affiliation":[{"name":"Department of Avian Diseases, College of Veterinary Medicine and Center for Avian Disease, Jeonbuk National University, Iksan 54596, Republic of Korea"},{"name":"College of Animal Science and Technology, Luoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Henan University of Science and Technology, Luoyang 471000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5389-6387","authenticated-orcid":false,"given":"Jong-Yeol","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Avian Diseases, College of Veterinary Medicine and Center for Avian Disease, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ri","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Avian Diseases, College of Veterinary Medicine and Center for Avian Disease, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyung-Kwan","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Avian Diseases, College of Veterinary Medicine and Center for Avian Disease, Jeonbuk National University, Iksan 54596, Republic of Korea"},{"name":"Bio Disease Control (BIOD) Co., Ltd., Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bai","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Avian Diseases, College of Veterinary Medicine and Center for Avian Disease, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Avian Diseases, College of Veterinary Medicine and Center for Avian Disease, Jeonbuk National University, Iksan 54596, Republic of Korea"},{"name":"Bio Disease Control (BIOD) Co., Ltd., Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se-Yeoun","family":"Cha","sequence":"additional","affiliation":[{"name":"Department of Avian Diseases, College of Veterinary Medicine and Center for Avian Disease, Jeonbuk National University, Iksan 54596, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Swayne, D.E. 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