{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:18:31Z","timestamp":1787498311934,"version":"build-2736575974"},"reference-count":46,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T00:00:00Z","timestamp":1710374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006533","name":"Ministry of Agriculture of the Czech Republic","doi-asserted-by":"publisher","award":["MZE-RO0723"],"award-info":[{"award-number":["MZE-RO0723"]}],"id":[{"id":"10.13039\/501100006533","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The aim of the study was to investigate the effect of calcareous marine algae (Lithotamium calcareum)-based rumen content buffer (CMA) included in concentrated feed within total mixed ration (TMR), fed to 34 peak lactation (87\u2013144 days in milk) Holstein dairy cows, randomized into two groups (group A, n = 17; group B, n = 17), wearing collars with accelerometers, and housed a in barn with automatic feed-weigh troughs. During the first phase P1, group A received TMR with CMA (TMR-E) and group B was fed TMR without the buffer (TMR-C). For P2, the treatments in the groups were exchanged. Feed intake, feeding time (FT), rumination time (RT), milk yield, milk composition, and rumen pH were measured by barn technologies, and rumen fluid and feces composition were analyzed in the laboratory. Differences between the TMR-E and TMR-C in most parameters under study were statistically insignificant, except overall FT and RT, which differed significantly between the groups. Group A, feeding at P1 by TMR-E, exhibited higher FT and RT than Group B (202 min\/cow\/day vs. 184 min\/cow\/day, and 486 min\/cow\/day vs. 428 min\/cow\/day, respectively). The RT significantly increased after switching from TMR-C to TMR-E. This implies that the buffer effect is delayed and persists after the withdrawal. In the group of cows that received control TMR without buffer in the first phase, RT and milk protein content increased significantly in the first week after the addition of buffer.<\/jats:p>","DOI":"10.3390\/ani14060897","type":"journal-article","created":{"date-parts":[[2024,3,15]],"date-time":"2024-03-15T14:05:25Z","timestamp":1710511525000},"page":"897","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effect of Calcareous Marine Algae Buffer on High-Producing Dairy Cows during Peak Lactation"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1172-8112","authenticated-orcid":false,"given":"Radko","family":"Lou\u010dka","sequence":"first","affiliation":[{"name":"Department of Animal Feeding and Nutrition, Institute of Animal Science, 104 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e1clav","family":"Jambor","sequence":"additional","affiliation":[{"name":"NutriVet Ltd., 691 23 Poho\u0159elice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hana","family":"Synkov\u00e1","sequence":"additional","affiliation":[{"name":"NutriVet Ltd., 691 23 Poho\u0159elice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Homolka","sequence":"additional","affiliation":[{"name":"Department of Animal Feeding and Nutrition, Institute of Animal Science, 104 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Kumprechtov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Animal Feeding and Nutrition, Institute of Animal Science, 104 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Koukolov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Animal Feeding and Nutrition, Institute of Animal Science, 104 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Kubelkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Animal Feeding and Nutrition, Institute of Animal Science, 104 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alena","family":"V\u00fdborn\u00e1","sequence":"additional","affiliation":[{"name":"Department of Animal Feeding and Nutrition, Institute of Animal Science, 104 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6644-6783","authenticated-orcid":false,"given":"Yvona","family":"Tyrolov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Animal Feeding and Nutrition, Institute of Animal Science, 104 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9914-2871","authenticated-orcid":false,"given":"Filip","family":"Jan\u010d\u00edk","sequence":"additional","affiliation":[{"name":"Department of Animal Feeding and Nutrition, Institute of Animal Science, 104 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,14]]},"reference":[{"key":"ref_1","first-page":"90","article-title":"Aspects of acidosis in ruminants with a focus on nutrition: A review","volume":"9","author":"Valente","year":"2017","journal-title":"J. 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