{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T08:46:20Z","timestamp":1790239580658,"version":"4.1.0"},"reference-count":28,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T00:00:00Z","timestamp":1710806400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Program of Guangzhou","award":["2023A04J0713"],"award-info":[{"award-number":["2023A04J0713"]}]},{"name":"Science and Technology Program of Guangzhou","award":["2024B03J1263"],"award-info":[{"award-number":["2024B03J1263"]}]},{"name":"Science and Technology Program of Guangzhou","award":["2023TD48"],"award-info":[{"award-number":["2023TD48"]}]},{"name":"Science and Technology Program of Guangzhou","award":["2022SPY00003"],"award-info":[{"award-number":["2022SPY00003"]}]},{"name":"Key Research and Development Program of Guangzhou City","award":["2023A04J0713"],"award-info":[{"award-number":["2023A04J0713"]}]},{"name":"Key Research and Development Program of Guangzhou City","award":["2024B03J1263"],"award-info":[{"award-number":["2024B03J1263"]}]},{"name":"Key Research and Development Program of Guangzhou City","award":["2023TD48"],"award-info":[{"award-number":["2023TD48"]}]},{"name":"Key Research and Development Program of Guangzhou City","award":["2022SPY00003"],"award-info":[{"award-number":["2022SPY00003"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["2023A04J0713"],"award-info":[{"award-number":["2023A04J0713"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["2024B03J1263"],"award-info":[{"award-number":["2024B03J1263"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["2023TD48"],"award-info":[{"award-number":["2023TD48"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["2022SPY00003"],"award-info":[{"award-number":["2022SPY00003"]}]},{"name":"Special-funds Project for Rural Revitalization Strategy of Guangdong Province","award":["2023A04J0713"],"award-info":[{"award-number":["2023A04J0713"]}]},{"name":"Special-funds Project for Rural Revitalization Strategy of Guangdong Province","award":["2024B03J1263"],"award-info":[{"award-number":["2024B03J1263"]}]},{"name":"Special-funds Project for Rural Revitalization Strategy of Guangdong Province","award":["2023TD48"],"award-info":[{"award-number":["2023TD48"]}]},{"name":"Special-funds Project for Rural Revitalization Strategy of Guangdong Province","award":["2022SPY00003"],"award-info":[{"award-number":["2022SPY00003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Aeromonas schubertii is a pathogen that severely affects aquatic animals, including the snakehead, Channa maculata. Lytic bacteriophages have been recognized as effective alternatives to antibiotics for controlling bacterial infections. However, there have been no reports of A. schubertii phages as far as we know. In this study, a lytic bacteriophage SD04, which could effectively infect A. schubertii, was isolated from pond water cultured with diseased snakehead. The SD04 phage formed small, round plaques on Petri dishes. Electron microscopy revealed a hexagonal head and a contractile tail. Based on its morphology, it may belong to the Myoviridae family. Two major protein bands with molecular weights of 50 and 38 kilodaltons were observed after the phage was subjected to SDS-PAGE. The phage showed a large average burst size, high specificity, and a broad host range. When stored at 4 \u00b0C, phage SD04 had high stability over 12 months and showed almost no variation within the first six months. All fish were healthy after both intraperitoneal injection and immersion administration of SD04, indicating the safety of the phage. After treatment with SD04, Channa maculata in both phage therapy groups and prevention groups showed high survival rates (i.e., 83.3 \u00b1 3.3% and 100 \u00b1 1.3%, respectively). Phage therapy inhibits bacterial growth in the liver, the target organ of the infected Channa maculat. The experimental results indicate the potential use of phage SD04 for preventing A. schubertii infection in Channa maculata.<\/jats:p>","DOI":"10.3390\/ani14060957","type":"journal-article","created":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T05:56:07Z","timestamp":1710914167000},"page":"957","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A Novel and Effective Therapeutic Method for Treating Aeromonas schubertii Infection in Channa maculata"],"prefix":"10.3390","volume":"14","author":[{"given":"Xia","family":"Luo","sequence":"first","affiliation":[{"name":"Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Aquatic Animal Immune and Sustainable Aquaculture, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoli","family":"Liao","sequence":"additional","affiliation":[{"name":"Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Aquatic Animal Immune and Sustainable Aquaculture, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaozhe","family":"Fu","sequence":"additional","affiliation":[{"name":"Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Aquatic Animal Immune and Sustainable Aquaculture, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongru","family":"Liang","sequence":"additional","affiliation":[{"name":"Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Aquatic Animal Immune and Sustainable Aquaculture, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinjie","family":"Niu","sequence":"additional","affiliation":[{"name":"Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Aquatic Animal Immune and Sustainable Aquaculture, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Lin","sequence":"additional","affiliation":[{"name":"Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Aquatic Animal Immune and Sustainable Aquaculture, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihui","family":"Liu","sequence":"additional","affiliation":[{"name":"Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Aquatic Animal Immune and Sustainable Aquaculture, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baofu","family":"Ma","sequence":"additional","affiliation":[{"name":"Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Aquatic Animal Immune and Sustainable Aquaculture, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningqiu","family":"Li","sequence":"additional","affiliation":[{"name":"Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Aquatic Animal Immune and Sustainable Aquaculture, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,19]]},"reference":[{"key":"ref_1","first-page":"1104","article-title":"Aeromonas schubertii: A Potential Pathogen for Freshwater Cultured Whiteleg Shrimp, Penaeus vannamei","volume":"67","author":"Cao","year":"2015","journal-title":"Isr. 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