{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T14:35:50Z","timestamp":1791988550330,"version":"4.4.0"},"reference-count":38,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T00:00:00Z","timestamp":1712620800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Key R&amp;D Program of China","award":["2022YFF1000304"],"award-info":[{"award-number":["2022YFF1000304"]}]},{"name":"the National Key R&amp;D Program of China","award":["32172960"],"award-info":[{"award-number":["32172960"]}]},{"name":"the National Key R&amp;D Program of China","award":["CARS-48"],"award-info":[{"award-number":["CARS-48"]}]},{"name":"the National Key R&amp;D Program of China","award":["2020TD26"],"award-info":[{"award-number":["2020TD26"]}]},{"name":"the National Key R&amp;D Program of China","award":["2022SDZG01"],"award-info":[{"award-number":["2022SDZG01"]}]},{"name":"the National Natural Science Foundation of China","award":["2022YFF1000304"],"award-info":[{"award-number":["2022YFF1000304"]}]},{"name":"the National Natural Science Foundation of China","award":["32172960"],"award-info":[{"award-number":["32172960"]}]},{"name":"the National Natural Science Foundation of China","award":["CARS-48"],"award-info":[{"award-number":["CARS-48"]}]},{"name":"the National Natural Science Foundation of China","award":["2020TD26"],"award-info":[{"award-number":["2020TD26"]}]},{"name":"the National Natural Science Foundation of China","award":["2022SDZG01"],"award-info":[{"award-number":["2022SDZG01"]}]},{"name":"the China Agriculture Research System of MOF and MARA","award":["2022YFF1000304"],"award-info":[{"award-number":["2022YFF1000304"]}]},{"name":"the China Agriculture Research System of MOF and MARA","award":["32172960"],"award-info":[{"award-number":["32172960"]}]},{"name":"the China Agriculture Research System of MOF and MARA","award":["CARS-48"],"award-info":[{"award-number":["CARS-48"]}]},{"name":"the China Agriculture Research System of MOF and MARA","award":["2020TD26"],"award-info":[{"award-number":["2020TD26"]}]},{"name":"the China Agriculture Research System of MOF and MARA","award":["2022SDZG01"],"award-info":[{"award-number":["2022SDZG01"]}]},{"name":"the Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["2022YFF1000304"],"award-info":[{"award-number":["2022YFF1000304"]}]},{"name":"the Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["32172960"],"award-info":[{"award-number":["32172960"]}]},{"name":"the Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["CARS-48"],"award-info":[{"award-number":["CARS-48"]}]},{"name":"the Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["2020TD26"],"award-info":[{"award-number":["2020TD26"]}]},{"name":"the Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["2022SDZG01"],"award-info":[{"award-number":["2022SDZG01"]}]},{"name":"the Open Competition Program of Top Ten Critical Priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province","award":["2022YFF1000304"],"award-info":[{"award-number":["2022YFF1000304"]}]},{"name":"the Open Competition Program of Top Ten Critical Priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province","award":["32172960"],"award-info":[{"award-number":["32172960"]}]},{"name":"the Open Competition Program of Top Ten Critical Priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province","award":["CARS-48"],"award-info":[{"award-number":["CARS-48"]}]},{"name":"the Open Competition Program of Top Ten Critical Priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province","award":["2020TD26"],"award-info":[{"award-number":["2020TD26"]}]},{"name":"the Open Competition Program of Top Ten Critical Priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province","award":["2022SDZG01"],"award-info":[{"award-number":["2022SDZG01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>White spot disease (WSD) outbreaks pose a significant threat to the Pacific white shrimp (Litopenaeus\u00a0vannamei) farming industry. The causative agent is the white spot syndrome virus (WSSV). There are no effective treatments for WSD so far. Therefore, understanding the resistance mechanisms of L. vannamei against the WSSV is crucial. C-type lectins (CTLs) are important pattern recognition receptors (PRRs) that promote agglutination, phagocytosis, encapsulation, bacteriostasis, and antiviral infections. This study cloned the C-type lectin domain family 4 member F (LvCLEC4F) from L. vannamei. LvCLEC4F contains a 492 bp open reading frame (ORF) encoding a protein of 163 amino acids, including a carbohydrate recognition domain (CRD). Following a challenge with the WSSV, the expression profile of LvCLEC4F was significantly altered. Using RNA interference (RNAi) technology, it was found that LvCLEC4F promotes WSSV replication and affects the expression levels of genes related to the regulation of apoptosis, signaling and cellular stress response, and immune defense. Meanwhile, the hemolymph agglutination phenomenon in vivo was weakened when LvCLEC4F was knocked down. These results indicated that LvCLEC4F may play an important role in the interaction between L. vannamei and WSSV.<\/jats:p>","DOI":"10.3390\/ani14081137","type":"journal-article","created":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T02:52:17Z","timestamp":1712631137000},"page":"1137","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular Characterization and Expression Analysis of the C-Type Lectin Domain Family 4 Member F in Litopenaeus\u00a0vannamei against White Spot Syndrome Virus"],"prefix":"10.3390","volume":"14","author":[{"given":"Qian","family":"Xue","sequence":"first","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"},{"name":"School of Fishery, Zhejiang Ocean University, Zhoushan 316021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingbing","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Luan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao 266237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Kong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao 266237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xupeng","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao 266237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianhong","family":"Meng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao 266237, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"738603","DOI":"10.1016\/j.aquaculture.2022.738603","article-title":"Comparative transcriptome analysis revealed that dietary zymosan-A improved the immunity of Penaeus vannamei by regulating the TLR signaling pathway","volume":"561","author":"Hou","year":"2022","journal-title":"Aquaculture"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.jip.2012.03.007","article-title":"Global transboundary disease politics: The OIE perspective","volume":"110","author":"Lightner","year":"2012","journal-title":"J. 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