{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:43:02Z","timestamp":1791996182368,"version":"4.4.0"},"reference-count":50,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T00:00:00Z","timestamp":1713139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Key R&amp;D program of Zhejiang Province","award":["2023C02026"],"award-info":[{"award-number":["2023C02026"]}]},{"name":"the Key R&amp;D program of Zhejiang Province","award":["CARS-41"],"award-info":[{"award-number":["CARS-41"]}]},{"name":"the Key R&amp;D program of Zhejiang Province","award":["2022ZDSJ0157"],"award-info":[{"award-number":["2022ZDSJ0157"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["2023C02026"],"award-info":[{"award-number":["2023C02026"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["CARS-41"],"award-info":[{"award-number":["CARS-41"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["2022ZDSJ0157"],"award-info":[{"award-number":["2022ZDSJ0157"]}]},{"name":"Hangzhou Agricultural and Social Development Key Research and Development project","award":["2023C02026"],"award-info":[{"award-number":["2023C02026"]}]},{"name":"Hangzhou Agricultural and Social Development Key Research and Development project","award":["CARS-41"],"award-info":[{"award-number":["CARS-41"]}]},{"name":"Hangzhou Agricultural and Social Development Key Research and Development project","award":["2022ZDSJ0157"],"award-info":[{"award-number":["2022ZDSJ0157"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study aimed to investigate the effects of different levels of xylanase supplementation in a wheat-based diet on growth performance, short-chain fatty acids, intestinal health, microbial composition, and serum metabolism. A total of 1200 male chicks were randomly assigned to four wheat-based diet treatments: Group C (adding 0 mg\/kg of xylanase), Group L (adding 50 mg\/kg of xylanase), Group M (adding 100 mg\/kg of xylanase), and Group H (adding 150 mg\/kg of xylanase). The experiment lasted for 56 days. The results indicated that Group H broilers experienced a decreased feed-to-gain ratio throughout the study period. Additionally, dietary supplementation with xylanase led to an increase in the physical barrier, as indicated by increased VH and VH\/CD in the gut (p &lt; 0.05). Furthermore, levels of D-lactic acid and endotoxin were reduced. Xylanase supplementation also increased the abundance of Muc-2, ZO-1, and Occludin (p &lt; 0.05). Moreover, xylanase supplementation enhanced the activity of sucrase and maltase in the duodenum (p &lt; 0.05), which may be attributable to the upregulation of the abundance of SI and MGA (p &lt; 0.05). Furthermore, xylanase addition promoted propionic acid produced by specific bacteria, such as Phascolarctobacterium, and influenced the microbial composition to some extent, promoting intestinal health. Additionally, 150 mg\/kg of xylanase supplementation increased the amino acid, peptide, and carbohydrate content and upregulated the metabolism of amino acids related to histidine, cysteine, methionine, and other pathways (p &lt; 0.05). These findings suggest adequate xylanase supplementation can enhance nutritional digestibility and absorption, improve growth performance, stimulate endogenous enzyme activity, optimize intestinal morphology and barrier function, and positively influence acid-producing bacteria and amino acid metabolic pathways.<\/jats:p>","DOI":"10.3390\/ani14081182","type":"journal-article","created":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T03:56:13Z","timestamp":1713153373000},"page":"1182","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Xylanase Supplement Enhances the Growth Performance of Broiler by Modulating Serum Metabolism, Intestinal Health, Short-Chain Fatty Acid Composition, and Microbiota"],"prefix":"10.3390","volume":"14","author":[{"given":"Xiaoli","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danlei","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yibin","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqing","family":"Ding","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Liang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyu","family":"Xie","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxia","family":"Wang","sequence":"additional","affiliation":[{"name":"Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuan","family":"Zhan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s12711-021-00608-3","article-title":"Identification of QTL regions and candidate genes for growth and feed efficiency in broilers","volume":"53","author":"Li","year":"2021","journal-title":"Genet. 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