{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:14:14Z","timestamp":1760109254306,"version":"build-2065373602"},"reference-count":40,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T00:00:00Z","timestamp":1713398400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005344","name":"Ministry for Environment, Agriculture, Conservation and Consumer Protection of the State of North Rhine-Westphalia","doi-asserted-by":"publisher","award":["17-02.04.01-14\/2019","491094227"],"award-info":[{"award-number":["17-02.04.01-14\/2019","491094227"]}],"id":[{"id":"10.13039\/501100005344","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)","award":["17-02.04.01-14\/2019","491094227"],"award-info":[{"award-number":["17-02.04.01-14\/2019","491094227"]}]},{"name":"University of Veterinary Medicine Hannover, Foundation","award":["17-02.04.01-14\/2019","491094227"],"award-info":[{"award-number":["17-02.04.01-14\/2019","491094227"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Chronic progressive lymphedema (CPL) is a prevalent and progressive disease in Rhenish German draught horses. The objective of our follow-up study was to evaluate the heritability of this disease in Rhenish German draught horses using pedigree-based and genomic relationship matrices. We employed linear and threshold animal models. Models included the random animal effect and effects of breeding association, coat colour, sex, and age within sex, and farm-related factors, on CPL scores. In addition, we estimated heritabilities in models assuming censoring for data when horses were below an age of 1\u201315 years. The heritabilities of CPL scores across all ages were 0.595 \u00b1 0.131 and 0.482 \u00b1 0.105 in the threshold and linear animal model with pedigree-based relationship matrices, respectively. The restriction of data to horses with a minimum age at examination or accounting for censored data in younger animals showed an increase in heritabilities of CPL scores up to 0.788 \u00b1 0.168 (threshold model) and 0.752 \u00b1 0.153 (linear model) at an age of 7\u20138 years. Analyses including genomic relationship matrices yielded very similar estimates, but with smaller standard errors than pedigree-based analyses. Heritabilities in threshold models for CPL prevalence (CPL-bin-score) and the number of affected limbs (CPL-bin-sum) were 0.176\u20130.189 \u00b1 0.061\u20130.064 and 0.375\u20130.433 \u00b1 0.164\u20130.170, respectively. We were able to show moderately to highly positive genetic correlations between the CPL score and cannon bone circumference (0.529\u20130.825), height at withers (0.338\u20130.555), and skinfold thickness (0.241\u20130.517). Using the dichotomous trait for the CPL score and the genomic relationship matrix resulted in corresponding estimates of 0.868, 0.793, and 0.784, respectively. This study showed the great importance of additive genetic variation influencing the expression of chronic progressive lymphedema in Rhenish German draught horses. Therefore, further research is warranted to implement breeding programmes in a small breeding population that exploit the potential of additive genetic differences among animals for reducing the prevalence and severity of lesions of this incurable disease.<\/jats:p>","DOI":"10.3390\/ani14081214","type":"journal-article","created":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T06:21:12Z","timestamp":1713421272000},"page":"1214","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Estimation of Genetic Parameters for Chronic Progressive Lymphedema and Body Traits in the Rhenish German Draught Horse"],"prefix":"10.3390","volume":"14","author":[{"given":"Johanna","family":"Sievers","sequence":"first","affiliation":[{"name":"Institute of Animal Breeding and Genetics, University of Veterinary Medicine Hannover (Foundation), 30559 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3516-836X","authenticated-orcid":false,"given":"Ottmar","family":"Distl","sequence":"additional","affiliation":[{"name":"Institute of Animal Breeding and Genetics, University of Veterinary Medicine Hannover (Foundation), 30559 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,18]]},"reference":[{"unstructured":"Gustine, G. (1910). Die Sogenannte Warzenmauke des Pferdes \u201cDermatitis Chronica Verrucosa\u201d. [Thesis, Doctor Medicinae Veterinariae, Vereinigte Medizinische Fakult\u00e4t der Gro\u00dfherz\u00f6glichen Hessischen Ludwigs-Universit\u00e4t zu Gie\u00dfen].","key":"ref_1"},{"key":"ref_2","first-page":"295","article-title":"Konstitutionelle Hauterkrankungen beim Pferd","volume":"37","year":"1937","journal-title":"Z. Tierz\u00fcchtg. Z\u00fcchtgsbiol."},{"key":"ref_3","first-page":"318","article-title":"Zur Kl\u00e4rung der Erb-und Umweltbeziehungen der Warzenmauke des Pferdes. Tier\u00e4rztl","volume":"4","author":"Weischer","year":"1949","journal-title":"Umschau"},{"key":"ref_4","first-page":"336","article-title":"Die Zuchtverwendungsdauer der Besch\u00e4ler des Landgest\u00fct Kreuz und ihre Abgangsursachen","volume":"10","author":"Wussow","year":"1954","journal-title":"Tierzucht"},{"key":"ref_5","first-page":"195","article-title":"Erbbiologische Untersuchungen \u00fcber die Mauke beim Kaltblutpferd","volume":"9","author":"Wussow","year":"1955","journal-title":"Tierzucht"},{"unstructured":"Duclos, P. (1972). Les Eaux-aux-Jambes du Cheval, Dermatose Hyperplasique des Membres. [Thesis, Doctor Medicinae Veterinariae, Ecole Nationale l\u2019Universite Claude Bernand de Lyon].","key":"ref_6"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/S0737-0806(01)70153-0","article-title":"Pastern dermatitis in Shires and Clydesdales","volume":"11","author":"Ferraro","year":"2001","journal-title":"J. Equine Vet. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1089\/153968503768330238","article-title":"Progressive swelling, hyperkeratosis, and fibrosis of distal limbs in Clydesdales, Shires, and Belgian draft horses, suggestive of primary lymphedema","volume":"1","author":"Affolter","year":"2003","journal-title":"Lymphat. Res. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1053\/jevs.2003.56","article-title":"Chronic progressive lymphedema in draft horses","volume":"23","author":"Ferraro","year":"2003","journal-title":"J. Equine Vet. Sci."},{"unstructured":"Wallraf, A. (2003). Populationsgenetische Untersuchung zum Auftreten von Mauke bei den Deutschen Kaltblutpferderassen. [Thesis, Doctor Medicinae Veterinariae, Tier\u00e4rztliche Hochschule Hannover].","key":"ref_10"},{"key":"ref_11","first-page":"148","article-title":"Analysis of the prevalence of pastern dermatitis in German Coldblood horse breeds","volume":"117","author":"Wallraf","year":"2004","journal-title":"Berl. Munch. Tierarztl. Wochenschr."},{"key":"ref_12","first-page":"246","article-title":"Populationsgenetische Untersuchung zum Auftreten von Mauke beim S\u00fcddeutschen Kaltblut","volume":"76","author":"Wallraf","year":"2004","journal-title":"Z\u00fcchtungskunde"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1111\/j.1365-3164.2005.00482.x","article-title":"Alterations of epidermal proliferation and cytokeratin expression in skin biopsies from heavy draught horses with chronic pastern dermatitis","volume":"16","author":"Geburek","year":"2005","journal-title":"Vet. Dermatol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1016\/j.cveq.2013.08.007","article-title":"Chronic progressive lymphedema in draft horses","volume":"29","author":"Affolter","year":"2013","journal-title":"Vet. Clin. N. Am. Equine Pract."},{"key":"ref_15","first-page":"119","article-title":"Chronic progressive lymphedema in the Belgian draft horse in Belgium: Clinical phenotyping, prevalence and risk factor analysis","volume":"83","author":"Janssens","year":"2014","journal-title":"Vlaams Diergeneeskd. Tijdschr."},{"key":"ref_16","first-page":"189","article-title":"Assessment of skinfold thickness as a factor related to chronic progressive lymphoedema in Belgian draught horses","volume":"76","author":"Peeters","year":"2010","journal-title":"Commun. Agric. Appl. Biol. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1111\/j.2042-3292.2011.00311.x","article-title":"Combined decongestive therapy including equine manual lymph drainage to assist management of chronic progressive lymphoedema in draught horses","volume":"24","author":"Powell","year":"2012","journal-title":"Equine Vet. Educ."},{"unstructured":"Bonnissent, C.R. (2012). \u00c9tiologie du Syndrome \u201cPattes \u00e0 Jus\u201d Chez le Baudet de Oitou et le Trait Mulassier Poitevin. [Ph.D. Thesis, \u00c9cole nationale v\u00e9t\u00e9rinaire d\u2019Alfort].","key":"ref_18"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1111\/evj.12256","article-title":"Chronic progressive lymphoedema in draught horses","volume":"47","author":"Janssens","year":"2015","journal-title":"Equine Vet. J."},{"doi-asserted-by":"crossref","unstructured":"Sievers, J., and Distl, O. (2023). Prevalence of Chronic Progressive Lymphedema in the Rhenish German Draught Horse. Animals, 13.","key":"ref_20","DOI":"10.3390\/ani13060999"},{"doi-asserted-by":"crossref","unstructured":"Brys, M., Claerebout, E., and Chiers, K. (2023). Chronic Progressive Lymphedema in Belgian Draft Horses: Understanding and Managing a Challenging Disease. Vet. Sci., 10.","key":"ref_21","DOI":"10.3390\/vetsci10050347"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1111\/jbg.12087","article-title":"Genetic parameters for chronic progressive lymphedema in Belgian Draught Horses","volume":"131","author":"Janssens","year":"2014","journal-title":"J. Anim. Breed. Genet."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"234-e51","DOI":"10.1111\/vde.12831","article-title":"Chronic progressive lymphoedema in Friesian horses: Suggestive phenotype of affected horses and genome-wide association study","volume":"31","author":"Affolter","year":"2020","journal-title":"Vet. Dermatol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/s00335-009-9244-z","article-title":"Whole-genome scan identifies quantitative trait loci for chronic pastern dermatitis in German draft horses","volume":"21","author":"Mittmann","year":"2010","journal-title":"Mamm. Genome"},{"key":"ref_25","first-page":"515","article-title":"Domestication of the horse: Results based on microsatellite and mitochondrial DNA markers","volume":"47","author":"Aberle","year":"2004","journal-title":"J. Arch. Anim. Breed."},{"key":"ref_26","first-page":"148","article-title":"Lymphoscintigraphy of draught horses with chronic progressive lymphoedema","volume":"38","author":"Affolter","year":"2006","journal-title":"Equine Vet. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1089\/lrb.2006.4.67","article-title":"Measurement of skin desmosine as an indicator of altered cutaneous elastin in draft horses with chronic progressive lymphedema","volume":"4","author":"Affolter","year":"2006","journal-title":"Lymphat. Res. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.jcpa.2008.10.009","article-title":"Quantitative and qualitative evaluation of dermal elastin of draught horses with chronic progressive lymphoedema","volume":"140","author":"Affolter","year":"2009","journal-title":"J. Comp. Pathol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"418","DOI":"10.2746\/042516407X205888","article-title":"Antibodies to elastin peptides in sera of Belgian Draught horses with chronic progressive lymphoedema","volume":"39","author":"Affolter","year":"2007","journal-title":"Equine Vet. J."},{"unstructured":"Kumpf, J. (1953). Beitrag zur Behandlung von Warzenmauke. [Thesis, Doctor Medicinae Veterinariae, Humboldt Universit\u00e4t zu Berlin].","key":"ref_30"},{"unstructured":"Groeneveld, E., Kovac, M., and Mielenz, N. (2008). VCE 6.0.2. Co-Variance Components Estimation Package, Institute of Farm Animal Genetics.","key":"ref_31"},{"unstructured":"Legarra, A., Varona, L., and L\u00f3pez de Maturana, E. (2023, September 01). TM Threshold Model. Available online: http:\/\/snp.toulouse.inrafr\/~alegarra\/manualtm.pdf.","key":"ref_32"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"s13742-015-0047-8","DOI":"10.1186\/s13742-015-0047-8","article-title":"Second-generation PLINK: Rising to the challenge of larger and richer datasets","volume":"4","author":"Chang","year":"2015","journal-title":"Gigascience"},{"unstructured":"Purcell, S., and Chang, C. (2023, September 01). PLINK 1.9. Available online: http:\/\/www.cog-genomics.org\/plink\/1.9\/.","key":"ref_34"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1016\/j.ajhg.2012.10.010","article-title":"Improved heritability estimation from genome-wide SNPs","volume":"91","author":"Speed","year":"2012","journal-title":"Am. J. Hum. Genet."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.2527\/jas1982.5451079x","article-title":"Theory and analysis of threshold characters","volume":"54","author":"Gianola","year":"1982","journal-title":"J. Anim. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1111\/jbg.12367","article-title":"Bias in heritability estimates from genomic restricted maximum likelihood methods under different genotyping strategies","volume":"136","author":"Cesarani","year":"2019","journal-title":"J. Anim. Breed. Genet."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1111\/j.1365-2052.2004.01166.x","article-title":"Genetic diversity in German draught horse breeds compared with a group of primitive, riding and wild horses by means of microsatellite DNA markers","volume":"35","author":"Aberle","year":"2004","journal-title":"Anim. Genet."},{"doi-asserted-by":"crossref","unstructured":"Kim, E.S., Cole, J.B., Huson, H., Wiggans, G.R., Tassel, C.P.V., Crooker, B.A., Liu, G., Da, Y., and Sonstegard, T.S. (2013). Effect of artificial selection on runs of homozygosity in U.S. Holstein cattle. PLoS ONE, 8.","key":"ref_39","DOI":"10.1371\/journal.pone.0080813"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4411","DOI":"10.3168\/jds.2007-0227","article-title":"Inbreeding effects on milk production, calving performance, fertility, and conformation in Irish Holstein-Friesians","volume":"90","author":"McParland","year":"2007","journal-title":"J. Dairy Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/8\/1214\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:29:49Z","timestamp":1760106589000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/8\/1214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,18]]},"references-count":40,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["ani14081214"],"URL":"https:\/\/doi.org\/10.3390\/ani14081214","relation":{},"ISSN":["2076-2615"],"issn-type":[{"type":"electronic","value":"2076-2615"}],"subject":[],"published":{"date-parts":[[2024,4,18]]}}}