{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:09:25Z","timestamp":1790143765100,"version":"4.0.1"},"reference-count":37,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T00:00:00Z","timestamp":1713916800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Science Foundation of China","award":["32273075"],"award-info":[{"award-number":["32273075"]}]},{"name":"the National Science Foundation of China","award":["31672616"],"award-info":[{"award-number":["31672616"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The average life of a dog is generally maintained at ten to fifteen years, and tumours are the predominant reason that leads to the death of dogs, especially canine mammary carcinoma. Therefore, early diagnosis of tumours is very important. In this study, tumor size, morphology, and texture could be seen through general clinical examination, tumor metastasis could be seen through imaging examination, inflammatory reactions could be seen through hematological examination, and abnormal cell morphology could be seen through cytological and histopathological examination. In the 269 malignant cases and 179 benign cases, we randomly selected 30 cases each, and an additional 30 healthy dogs were selected for the experiment (healthy dogs: dogs in good physical condition without any tumor or other diseases). We used RT-qPCR and ELISA to determine the relative expression of vascular endothelial growth factor (VEGF), tumor protein P53 (P53), serum ferritin (SF), and NOD-like receptor protein 3 (NLRP3) in 30 healthy dogs, 30 dogs with benign mammary tumours, and 30 dogs with malignant mammary tumours. In the results, the same expression trend was obtained both in serum and tissues, and the expression of the four markers was the highest in malignant mammary tumours, with highly significant differences compared with the benign and healthy\/paracancerous groups. By plotting the ROC curves, it was found that the results of combined tests were better than a single test and the combination of the four markers was the best for the early diagnosis. In conclusion, this can assist the clinical early diagnosis to a certain extent, and also provides some references and assistance for the development of tumor detection kits in clinical practice.<\/jats:p>","DOI":"10.3390\/ani14091272","type":"journal-article","created":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T07:38:51Z","timestamp":1713944331000},"page":"1272","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Diagnosis of Canine Tumours and the Value of Combined Detection of VEGF, P53, SF and NLRP3 for the Early Diagnosis of Canine Mammary Carcinoma"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-0131-9420","authenticated-orcid":false,"given":"Ning-Yu","family":"Yang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Jilin University, No. 5333 Xi\u2019an Road, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Hua","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jilin University, No. 5333 Xi\u2019an Road, Changchun 130062, China"},{"name":"College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jilin University, No. 5333 Xi\u2019an Road, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jilin University, No. 5333 Xi\u2019an Road, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang-Hong","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jilin University, No. 5333 Xi\u2019an Road, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1177\/0300985810393258","article-title":"Classification and grading of canine mammary tumors","volume":"48","author":"Goldschmidt","year":"2011","journal-title":"Vet. Pathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1177\/0300985816638721","article-title":"Cytologic Criteria for Mast Cell Tumor Grading in Dogs With Evaluation of Clinical Outcome","volume":"53","author":"Camus","year":"2016","journal-title":"Vet. Pathol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/j.cvsm.2019.04.002","article-title":"Diagnosis and Prognosis of Canine Cutaneous Mast Cell Tumors","volume":"49","author":"Kiupel","year":"2019","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1093\/jnci\/djy088","article-title":"Reporting Recommendations for Tumor Marker Prognostic Studies (REMARK): An Abridged Explanation and Elaboration","volume":"110","author":"Sauerbrei","year":"2018","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1159\/000074423","article-title":"Tumor marker workshops","volume":"24","author":"Rye","year":"2003","journal-title":"Tumour Biol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Faria, S.S., Costantini, S., de Lima, V.C.C., de Andrade, V.P., Rialland, M., Cedric, R., Budillon, A., and Magalh\u00e3es, K.G. (2021). NLRP3 inflammasome-mediated cytokine production and pyroptosis cell death in breast cancer. J. Biomed. Sci., 28.","DOI":"10.1186\/s12929-021-00724-8"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1038\/labinvest.2013.126","article-title":"Deregulation of the NLRP3 inflammasome in hepatic parenchymal cells during liver cancer progression","volume":"94","author":"Wei","year":"2014","journal-title":"Lab. Investig."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"724923","DOI":"10.3389\/fphar.2021.724923","article-title":"NLRP3 Overexpression Associated With Poor Prognosis and Presented as an Effective Therapeutic Target in Osteosarcoma","volume":"12","author":"Huang","year":"2021","journal-title":"Front. Pharmacol."},{"key":"ref_9","first-page":"1215","article-title":"Overexpression of the p53 gene product in canine mammary tumors","volume":"8","author":"Haga","year":"2001","journal-title":"Oncol. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"10617","DOI":"10.1007\/s11033-023-08863-x","article-title":"Research progress of good markers for canine mammary carcinoma","volume":"50","author":"Yang","year":"2023","journal-title":"Mol. Biol. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1177\/1040638717721730","article-title":"TP53 gene expression levels and tumor aggressiveness in canine mammary carcinomas","volume":"29","author":"Oliveira","year":"2017","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4168","DOI":"10.2741\/2378","article-title":"p53 in breast cancer: Mutation and countermeasures","volume":"12","author":"Kumar","year":"2007","journal-title":"Front. Biosci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"321","DOI":"10.4142\/jvs.2002.3.4.321","article-title":"Mutations of p53 tumor suppressor gene in spontaneous canine mammary tumors","volume":"3","author":"Lee","year":"2002","journal-title":"J. Vet. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Carvalho, M.I., Silva-Carvalho, R., Pires, I., Prada, J., Bianchini, R., Jensen-Jarolim, E., and Queiroga, F.L. (2016). A Comparative Approach of Tumor-Associated Inflammation in Mammary Cancer between Humans and Dogs. Biomed. Res. Int., 2016.","DOI":"10.1155\/2016\/4917387"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.jcpa.2009.06.004","article-title":"Expression of vascular endothelial growth factor in canine inflammatory and non-inflammatory mammary carcinoma","volume":"142","author":"Millanta","year":"2010","journal-title":"J. Comp. Pathol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.jcpa.2010.04.006","article-title":"Immunohistochemical expression of vascular endothelial growth factor in canine mammary tumours","volume":"143","author":"Santos","year":"2010","journal-title":"J. Comp. Pathol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.jcpa.2013.09.005","article-title":"Angiogenesis in canine mammary tumours: A morphometric and prognostic study","volume":"150","author":"Sleeckx","year":"2014","journal-title":"J. Comp. Pathol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1354\/vp.39-4-488","article-title":"Expression of vascular endothelial growth factor in canine mammary tumors","volume":"39","author":"Restucci","year":"2002","journal-title":"Vet. Pathol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Pimentel, P.A.B., Giuliano, A., B\u0119czkowski, P.M., and Horta, R.D.S. (2023). Molecular Profile of Canine Hemangiosarcoma and Potential Novel Therapeutic Targets. Vet. Sci., 10.","DOI":"10.20944\/preprints202305.1418.v1"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"6651","DOI":"10.1038\/s41598-021-85029-4","article-title":"Combined detection of CA15-3, CEA, and SF in serum and tissue of canine mammary gland tumor patients","volume":"11","author":"Fan","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1007\/s12032-009-9203-2","article-title":"Altered iron metabolism, inflammation, transferrin receptors, and ferritin expression in non-small-cell lung cancer","volume":"27","author":"Kukulj","year":"2010","journal-title":"Med. Oncol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Yu, C., Zheng, H., Liu, X., and Xie, G. (2022). The Analysis of E-Cadherin, N-Cadherin, Vimentin, HER-2, CEA, CA15-3 and SF Expression in the Diagnosis of Canine Mammary Tumors. Animals, 12.","DOI":"10.3390\/ani12213050"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Fredenberg, E., Willsher, P., Moa, E., Dance, D.R., Young, K.C., and Wallis, M.G. (2018). Measurement of breast-tissue x-ray attenuation by spectral imaging: Fresh and fixed normal and malignant tissue. Phys. Med. Biol., 63.","DOI":"10.1088\/1361-6560\/aaea83"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"106798","DOI":"10.1016\/j.ultras.2022.106798","article-title":"Ultrasonic attenuation of canine mammary tumours","volume":"125","author":"Filippou","year":"2022","journal-title":"Ultrasonics"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1111\/vru.12798","article-title":"CT imaging features of canine thymomas","volume":"60","author":"Randall","year":"2019","journal-title":"Vet. Radiol. Ultrasound"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1053\/j.semdp.2020.04.006","article-title":"Fine needle aspiration of thymic epithelial neoplasms and non-neoplastic lesions","volume":"37","author":"Illei","year":"2020","journal-title":"Semin. Diagn. Pathol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"843390","DOI":"10.3389\/fvets.2022.843390","article-title":"Epidemiological Investigation of Canine Mammary Tumors in Mainland China Between 2017 and 2021","volume":"9","author":"Zheng","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4223","DOI":"10.1200\/JCO.2012.42.6858","article-title":"Publication of tumor marker research results: The necessity for complete and transparent reporting","volume":"30","author":"McShane","year":"2012","journal-title":"J. Clin. Oncol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1556\/004.2017.036","article-title":"Expression of iNOS, COX-2 and VEGF in canine mammary tumours and non-neoplastic mammary glands: Association with clinicopathological features and tumour grade","volume":"65","author":"Anadol","year":"2017","journal-title":"Acta Vet. Hung."},{"key":"ref_30","first-page":"1109","article-title":"Immunohistochemical expression of vascular endothelial growth factor and vascular endothelial growth factor receptor-2 in canine simple mammary gland adenocarcinomas","volume":"51","author":"Haines","year":"2010","journal-title":"Can. Vet. J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"262","DOI":"10.3389\/fphar.2015.00262","article-title":"NLRP3 inflammasome and its inhibitors: A review","volume":"6","author":"Shao","year":"2015","journal-title":"Front. Pharmacol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1016\/j.bbagen.2010.03.011","article-title":"Serum ferritin: Past, present and future","volume":"1800","author":"Wang","year":"2010","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_33","first-page":"6473","article-title":"Prognostic role of pre-operative serum ferritin level in stage 2 colon cancer","volume":"25","author":"Demir","year":"2021","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1093\/jmcb\/mjaa040","article-title":"Gain-of-function mutant p53 in cancer progression and therapy","volume":"12","author":"Zhang","year":"2020","journal-title":"J. Mol. Cell Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1038\/s41418-018-0246-9","article-title":"Mutant p53 as a guardian of the cancer cell","volume":"26","author":"Mantovani","year":"2019","journal-title":"Cell Death Differ."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Ferreira, T., Gama, A., Seixas, F., Faustino-Rocha, A.I., Lopes, C., Gaspar, V.M., Mano, J.F., Medeiros, R., and Oliveira, P.A. (2023). Mammary Glands of Women, Female Dogs and Female Rats: Similarities and Differences to Be Considered in Breast Cancer Research. Vet. Sci., 10.","DOI":"10.3390\/vetsci10060379"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"563779","DOI":"10.3389\/fonc.2020.563779","article-title":"Triple-Negative Breast Cancer Comparison With Canine Mammary Tumors From Light Microscopy to Molecular Pathology","volume":"10","author":"Ciani","year":"2020","journal-title":"Front. Oncol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/9\/1272\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:33:08Z","timestamp":1760106788000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/14\/9\/1272"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,24]]},"references-count":37,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,5]]}},"alternative-id":["ani14091272"],"URL":"https:\/\/doi.org\/10.3390\/ani14091272","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,24]]}}}