{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T02:46:57Z","timestamp":1791600417365,"version":"4.3.4"},"reference-count":103,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T00:00:00Z","timestamp":1713916800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University Grant Commission (UGC), Bangladesh","award":["37.01.0000.073.03.007.20.133"],"award-info":[{"award-number":["37.01.0000.073.03.007.20.133"]}]},{"name":"University Grant Commission (UGC), Bangladesh","award":["PJ010859012016"],"award-info":[{"award-number":["PJ010859012016"]}]},{"name":"Cooperative Research Program for Agricultural Science &amp; Technology Development","award":["37.01.0000.073.03.007.20.133"],"award-info":[{"award-number":["37.01.0000.073.03.007.20.133"]}]},{"name":"Cooperative Research Program for Agricultural Science &amp; Technology Development","award":["PJ010859012016"],"award-info":[{"award-number":["PJ010859012016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Lumpy skin disease (LSD), caused by a virus within the Poxviridae family and Capripoxvirus genus, induces nodular skin lesions in cattle. This spreads through direct contact and insect vectors, significantly affecting global cattle farming. Despite the availability of vaccines, their efficacy is limited by poor prophylaxis and adverse effects. Our study aimed to identify the potential inhibitors targeting the LSDV-encoded DNA polymerase protein (gene LSDV039) for further investigation through comprehensive analysis and computational methods. Virtual screening revealed rhein and taxifolin as being potent binders among 380 phytocompounds, with respective affinities of \u22128.97 and \u22127.20 kcal\/mol. Canagliflozin and tepotinib exhibited strong affinities (\u22129.86 and \u22128.86 kcal\/mol) among 718 FDA-approved antiviral drugs. Simulating the molecular dynamics of canagliflozin, tepotinib, rhein, and taxifolin highlighted taxifolin\u2019s superior stability and binding energy. Rhein displayed compactness in RMSD and RMSF, but fluctuated in Rg and SASA, while canagliflozin demonstrated stability compared to tepotinib. This study highlights the promising potential of using repurposed drugs and phytocompounds as potential LSD therapeutics. However, extensive validation through in vitro and in vivo testing and clinical trials is crucial for their practical application.<\/jats:p>","DOI":"10.3390\/ani14091283","type":"journal-article","created":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T05:26:13Z","timestamp":1714022773000},"page":"1283","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Potential Inhibitors of Lumpy Skin Disease\u2019s Viral Protein (DNA Polymerase): A Combination of Bioinformatics Approaches"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-5804-2400","authenticated-orcid":false,"given":"Sabbir","family":"Zia","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3307-2989","authenticated-orcid":false,"given":"Md-Mehedi","family":"Sumon","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md-Ashiqur","family":"Ashik","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0463-5252","authenticated-orcid":false,"given":"Abul","family":"Basar","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9108-3048","authenticated-orcid":false,"given":"Sangjin","family":"Lim","sequence":"additional","affiliation":[{"name":"College of Forest & Environmental Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5743-5396","authenticated-orcid":false,"given":"Yeonsu","family":"Oh","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yungchul","family":"Park","sequence":"additional","affiliation":[{"name":"College of Forest & Environmental Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4066-1957","authenticated-orcid":false,"given":"Md-Mafizur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"322","DOI":"10.5455\/jabet.2021.d133","article-title":"An updated review on lumpy skin disease: Perspective of Southeast Asian countries","volume":"4","author":"Das","year":"2021","journal-title":"J. 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